• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Slide 1
Slide 1

...  Post-translational modifications (PTMs)  Covalent processing events that change the properties of a protein  proteolytic cleavage  addition of a modifying group to one or more amino acids  Determine its activity state, localization, turnover, interactions with other proteins  Mass spectrometr ...
Computation in Biology
Computation in Biology

... ligands generated using RACHEL that are predicted to be active. Hydrogen bonds between the ligands and residues are indicated by dashed yellow lines. The surface was rendered using MOLCAD . Dark purple regions contain a greater Acceptor/donor density and light purple regions indicate areas where hyd ...
Analytical Questions
Analytical Questions

... 5. Archibald Garrod is the physician who was credited with noting the hereditary nature of the disease, Alcaptonuria. Being familiar with Mendel’s work, he proposed that a single recessive gene caused the disease. He also hypothesized that the disorder was caused by a defect in a metabolic pathway a ...
Clean Lean Protein Powde
Clean Lean Protein Powde

... It’s the best choice for everyone Clean Lean Protein is great for kids and adults alike. It has something for everyone. It has The Alkaline Advantage and high protein content for athletes. Its low allergenic profile is perfect for allergy sufferers and its low fat profile is great for those watching ...
No Slide Title
No Slide Title

... +Hospital-acquired infections ...
File
File

... As we know that large number of proteins/enzymes found in nature, which fold into a variety of different structures and carry out a huge diversity of functions. Protein engineering attempts to design protein/enzyme structures, including those having particular functions. Therefore targets for enzyme ...
Fig. 8.1. Amino acid structure
Fig. 8.1. Amino acid structure

... interactions between amino acids folding to give 3-D structure domains ...
Plant Molecular Biology
Plant Molecular Biology

... Finally, one gene (DRP6) is classified in the subfamily of unknown dynamin-related genes. It encodes a polypeptide of 251 amino acids which is much smaller than other DRPs. It is not known if DRP6 gene is transcribed, and if its encoded protein is a GTPase. We hope that these changes will make it ea ...
Constitutive expression of Vitis vinifera thaumatin
Constitutive expression of Vitis vinifera thaumatin

... Spores were collected from a culture plate of E. ampelina and suspended in 1 mL Czapek-Dox broth. After estimating the spore density, a stock suspension of 500 000 spores per mL in sterile distilled water was made. Spore germination was tested in 96-well microwell plates by diluting 100 µL of spore ...
L5 Food proteins - e
L5 Food proteins - e

... high quality because they contain all the essential amino acids in proportions similar to those required for synthesis of human tissue proteins [Note: Gelatin prepared from animal collagen is an exception; it has a low biological value as a result of deficiencies in several essential amino acids.] ...
Lecture 5: Major Nutrient Groups
Lecture 5: Major Nutrient Groups

... used CAAs  CAAs produced by bacteria  can help reduce formulation cost of feeds because they are 99% digestible  for most aquatics, no more than 12.5% of AA-N  problems: reduced palatability, leaching, rapid uptake ...
PowerPoint Presentation - AGRI-MIS
PowerPoint Presentation - AGRI-MIS

... C20-GA and C19-GA GA derivatives by modification of 4 rings * C20 oxidation: CH3 CH2OH CHO COOH * Hydroxylation at C2 C3 and C13: ...
An abundant TIP expressed in mature highly vacuolated cells
An abundant TIP expressed in mature highly vacuolated cells

... plasma membrane. One aquaporin, NOD26, has been localized to the peribacteroid membrane of soybean nodules (Rivers et al., 1997). In Arabidopsis, there are at least 30 expressed members of the major intrinsic protein (MIP) family (U. Johanson et al., unpublished data). According to amino acid sequen ...
Hormones in intermediary metabolism
Hormones in intermediary metabolism

... • ↓ lipids and cholesterol in the blood (↓ cholesterol synthesis and ↑ secretion of bile) ...
Surface expression of the conserved ribosomal protein P0 on
Surface expression of the conserved ribosomal protein P0 on

... India [1]. Purified IgG from rabbit sera, raised against different domains of the PfP0 protein, show no effect on the intraerythrocytic stages of Plasmodium falciparum in culture, but inhibit the invasion of erythrocytes by merozoites in a concentration dependent manner [2,3]. The same antibodies ag ...
Protein structure - Primary
Protein structure - Primary

... Protein + Lipid = Lipoprotein (lecithin) Protein + Phosphate = Phosphoprotein (caesin) Protein + nucleic acid = Nucleoprotein (DNA) Protein + Colour Pigment = Chromoprotein (Haemoglobin) ...
Intragenomic Spread of Plastid-Targeting
Intragenomic Spread of Plastid-Targeting

... FIG. 1. (A) N-terminal sequences consisting of a bipartite plastid targeting signal (signal peptide and transit peptide) and a highly conserved domain among six non-homologous proteins and three recent paralogs (FBA I, PK, and CcP). The arrowhead indicates the predicted signal peptide cleavage sites ...
MSG
MSG

... and has no distinct flavor, although some describe a salty/sweet flavor. Its chemical formulation has been modified and it is technically known as D-glutamic acid. It also contains some L-glutamic acid, pyroglutamic acid, and other contaminants This factory made version causes sensitive individuals ...
PEPCK: a model of eukaryotic gene expression
PEPCK: a model of eukaryotic gene expression

... In the normal fed state PEPCK mRNA in the liver is rapidly turned over. This is typical for a sequence which is regulated at the level of gene expression. Why? In the fed state insulin is released by the pancreas and this suppresses transcription of PEPCK, the mRNA is unstable so very little PEPCK p ...
2O2 - + 2H+ ------> H2O2 + O2 M3+ + O2 - ------> M2+ + O2 i
2O2 - + 2H+ ------> H2O2 + O2 M3+ + O2 - ------> M2+ + O2 i

... E.coli
MnSOD
and
FeSOD

 E.coli contains both MnSOD and FeSOD. As well as having a high degree of homology between themselves (in the left figure, FeSOD is yellow, MnSOD is blue), all mononuclear SODs like these are all very similar on a structural level, though some are active as dimers (as in E.co ...
Protein Function
Protein Function

... Lysozyme lowers the activation energy by straining the bond and by stabilizing the transition state (by allowing a temporary covalent bond between the sugar and the enzyme molecule). Also, in the microenvironment on the reaction site, note that glutamic acid is in the –COOH form and aspartic acid is ...
lecture2-Proteins2014-08
lecture2-Proteins2014-08

... – Heat: disrupts hydrogen bonding – Change in pH: alters ionization states of aa – Detergents: interfere with hydrophobic interactions – Chaotropic agents: ions or small organic molecules that disrupt hydrophobic interactions ...
Identification and Characterization of the Potato Leafroll Virus
Identification and Characterization of the Potato Leafroll Virus

... The start codons used for the 23K ORF and 17K ORF are the first A U G codons following a stop codon ('k) in the same frame. ...
A new phagemid vector for positive selection of recombinants based
A new phagemid vector for positive selection of recombinants based

... of the Val-36 codon, a site which does not alter the lethal e¡ects of barnase [1]. Hence, this pMT440 contained the barnase-barstar cassette, the entire pUC19 polylinker under the control of the tac promoter and a vector fragment containing the ori and the ampicillin-resistance gene of the pUC19 pla ...
04b AP Bio The Structure and Function of Proteins and Nucleic
04b AP Bio The Structure and Function of Proteins and Nucleic

... • At first, all we have is a string of AA’s bound with peptide bonds. • Once the string of AA’s interacts with itself and its environment (often aqueous), then we have a functional protein that consists of one or more polypeptides precisely twisted, folded, and coiled into a unique shape • The seque ...
< 1 ... 145 146 147 148 149 150 151 152 153 ... 221 >

Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report