Structure-Function Analysis of Mouse Interferon Alpha Species
... Antiviral properties of the MulFN-~IO protein To obtain information on the antiviral properties of the MulFN-~10 protein, the gene was inserted into the eukaryotic expression vector pSV328A (Van Heuvel et al., 1986) which contains the origin of replication and early promoter of simian virus 40 (SV40 ...
... Antiviral properties of the MulFN-~IO protein To obtain information on the antiviral properties of the MulFN-~10 protein, the gene was inserted into the eukaryotic expression vector pSV328A (Van Heuvel et al., 1986) which contains the origin of replication and early promoter of simian virus 40 (SV40 ...
Intracellular metal transport proteins
... in the proximal duodenum. Ferroportin1 was cloned from zebrafish, mouse, and human. This protein has 570 amino acids with 10 putative membrane domains and its mRNA was found to be expressed at the basolateral surface of the duodenal enterocyte. Thus, ferroportin1 was proposed to be involved in the tr ...
... in the proximal duodenum. Ferroportin1 was cloned from zebrafish, mouse, and human. This protein has 570 amino acids with 10 putative membrane domains and its mRNA was found to be expressed at the basolateral surface of the duodenal enterocyte. Thus, ferroportin1 was proposed to be involved in the tr ...
The Pentatricopeptide Repeat Protein OTP87 Is Essential for RNA
... Genome has been completely sequenced (transcriptomics and proteomics also well developed) ...
... Genome has been completely sequenced (transcriptomics and proteomics also well developed) ...
Heat shock Proteins (HSPs)
... Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat, high pressures, and toxic compounds. It is also one of the most abundant cellular proteins found under nonstress conditions ...
... Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat, high pressures, and toxic compounds. It is also one of the most abundant cellular proteins found under nonstress conditions ...
II. The selected examples
... 5. In fact, transcription of the late genes by the gene 1 product may help pull the DNA into the cell, causing sequential gene expression. 6. The specificity of phage RNA polymerase for their own promoter has been exploited in many applications in molecular genetics: (1) T7 phage based expression ve ...
... 5. In fact, transcription of the late genes by the gene 1 product may help pull the DNA into the cell, causing sequential gene expression. 6. The specificity of phage RNA polymerase for their own promoter has been exploited in many applications in molecular genetics: (1) T7 phage based expression ve ...
Institute for Plant Physiology and Microbiology - diss.fu
... Ismail A. M., Hall A. E., Close T. J. (1999) Purification and partial characterization of a dehydrin involved in chilling tolerance during seedling emergence of cowpea. Plant Physiol. 120:237-244 Jepson S. G. and Close T. J.(1995). “Purification of a Maize Dehydrin Protein Expressed in Escherichia-C ...
... Ismail A. M., Hall A. E., Close T. J. (1999) Purification and partial characterization of a dehydrin involved in chilling tolerance during seedling emergence of cowpea. Plant Physiol. 120:237-244 Jepson S. G. and Close T. J.(1995). “Purification of a Maize Dehydrin Protein Expressed in Escherichia-C ...
Molecular characterisation of RecQ homologues in Arabidopsis
... a powerful tool for identifying putative homologous proteins by database searches with sequence motives of genes of known function from different organisms. Such a database search using TBLASTN with the conserved helicase motives of the Bloom and the Werner syndrome protein sequences of H.sapiens re ...
... a powerful tool for identifying putative homologous proteins by database searches with sequence motives of genes of known function from different organisms. Such a database search using TBLASTN with the conserved helicase motives of the Bloom and the Werner syndrome protein sequences of H.sapiens re ...
Proteomics pathway Most common properties of proteins
... This procedure is very important and must: must: • Solubilise as many proteins as possible including hydrophobic species • Prevent protein aggregates and hydrophobic interactions. This includes denaturing the proteins to break non-covalent interactions, to break disulfide bonds and to disrupt non-co ...
... This procedure is very important and must: must: • Solubilise as many proteins as possible including hydrophobic species • Prevent protein aggregates and hydrophobic interactions. This includes denaturing the proteins to break non-covalent interactions, to break disulfide bonds and to disrupt non-co ...
E. coli
... Biological significance: • Many expressed genes in bacteria are transcribed only once per cell cycle • Some cells fail to produce an essential message during a cycle, and so must depend on existing messages and/or proteins for survival ...
... Biological significance: • Many expressed genes in bacteria are transcribed only once per cell cycle • Some cells fail to produce an essential message during a cycle, and so must depend on existing messages and/or proteins for survival ...
Big Biology meets Obvious
... Genomics group founded in 1998 to begin providing large-scale DNA sequencing services ...
... Genomics group founded in 1998 to begin providing large-scale DNA sequencing services ...
Supplementary information - Word file (31 KB )
... DNA. The “downstream” Xtwn promoter primer (8) was used with the primer 5’GTAAGcgaccttttgcaAGGTGTCATGTaccgag-3’to produce a 3’ fragment containing a mutation in Lef1 site 4 (Figure 1). Lowercase letters represent nucleotides changes that are different from the wild-type promoter. In a second reactio ...
... DNA. The “downstream” Xtwn promoter primer (8) was used with the primer 5’GTAAGcgaccttttgcaAGGTGTCATGTaccgag-3’to produce a 3’ fragment containing a mutation in Lef1 site 4 (Figure 1). Lowercase letters represent nucleotides changes that are different from the wild-type promoter. In a second reactio ...
Supplementary Material and Methods
... Optiwell microtiter plates (PerkinElmer). All components were diluted to the indicated concentrations in assay buffer (25 mM Tris pH7.4, 150 mM NaCl, 1 mM DTT, 0.1% (v/v) Tween-20 and 0.1% (w/v) Bovine Serum Albumin (BSA). Optimal binding concentrations for MLL-Menin, Menin-LEDGF/p75 and LEDGF/p75-M ...
... Optiwell microtiter plates (PerkinElmer). All components were diluted to the indicated concentrations in assay buffer (25 mM Tris pH7.4, 150 mM NaCl, 1 mM DTT, 0.1% (v/v) Tween-20 and 0.1% (w/v) Bovine Serum Albumin (BSA). Optimal binding concentrations for MLL-Menin, Menin-LEDGF/p75 and LEDGF/p75-M ...
Monomeric state and ligand binding of recombinant GABA transporter Xiao-Dan Li
... and detergents from Anatrace (Maumee, OH, USA). All other chemicals were from Sigma (St. Louis, MO, USA) and were of analytical grade or better. 2.2. PCR cloning The GabP gene was ampli¢ed by PCR, using chromosome DNA puri¢ed from E. coli DH5K cell lysate as the template. Forward and reverse primers ...
... and detergents from Anatrace (Maumee, OH, USA). All other chemicals were from Sigma (St. Louis, MO, USA) and were of analytical grade or better. 2.2. PCR cloning The GabP gene was ampli¢ed by PCR, using chromosome DNA puri¢ed from E. coli DH5K cell lysate as the template. Forward and reverse primers ...
Complete nucleotide sequence and genome organization of a
... The 3’-terminal non-translated region in the cr-TMV genome can be folded into seven potentially stable pseudoknots and one additonal pseudoknot at the 3’-end of the CP gene compared to only five pseudoknots found in the Ul-TMV sequence (Fig. 4). The strongest structural conse~ation between the cr-TM ...
... The 3’-terminal non-translated region in the cr-TMV genome can be folded into seven potentially stable pseudoknots and one additonal pseudoknot at the 3’-end of the CP gene compared to only five pseudoknots found in the Ul-TMV sequence (Fig. 4). The strongest structural conse~ation between the cr-TM ...
Varicella-Zoster Virus Specifies a Thymidylate Synthetase
... No information on the kinetic class and structure of the m R N A specified by VZV gene 13 is yet available, but the D N A sequence indicates that the protein-coding region of the gene contains no introns, and that the m R N A is polyadenylated about 70 nucleotides downstream from the translational s ...
... No information on the kinetic class and structure of the m R N A specified by VZV gene 13 is yet available, but the D N A sequence indicates that the protein-coding region of the gene contains no introns, and that the m R N A is polyadenylated about 70 nucleotides downstream from the translational s ...
Supplementary Information (docx 4776K)
... Mn(III) was also monitored during Mn(II) oxidation by the co-culture of strain Arthrobacter and strain Sphingopyxis. Ligand-binding complex P2O74- (PP) was selected to complex Mn(III) by forming stable Mn(III)-PP complex, which has max absorbance at 258 nm (ε = 6,750 M-1). The co-culture was first c ...
... Mn(III) was also monitored during Mn(II) oxidation by the co-culture of strain Arthrobacter and strain Sphingopyxis. Ligand-binding complex P2O74- (PP) was selected to complex Mn(III) by forming stable Mn(III)-PP complex, which has max absorbance at 258 nm (ε = 6,750 M-1). The co-culture was first c ...
Constructing and Screening a Recombinant DNA Library
... a) When you construct the yeast genomic library in E. coli, what type of yeast would you choose as the donor for the genomic DNA? A wildtype yeast strain or a lysine prototroph. b) Once the DNA is isolated from the yeast cells, what would be your next step in preparing the DNA for use in constructin ...
... a) When you construct the yeast genomic library in E. coli, what type of yeast would you choose as the donor for the genomic DNA? A wildtype yeast strain or a lysine prototroph. b) Once the DNA is isolated from the yeast cells, what would be your next step in preparing the DNA for use in constructin ...
Aligning Sequences…. - School of Biotechnology, Devi Ahilya
... -PROSITE/InterPro-protein families characterized by presence of single most conserved motif (domains) by multiple sequence alignment -PRINTS-protein families are characterized by several conserved motifs to develop a fingerprint or signature for a particular family. BLOCKS and Pfam Profiles-variable ...
... -PROSITE/InterPro-protein families characterized by presence of single most conserved motif (domains) by multiple sequence alignment -PRINTS-protein families are characterized by several conserved motifs to develop a fingerprint or signature for a particular family. BLOCKS and Pfam Profiles-variable ...
PHS 398 (Rev. 9/04), Biographical Sketch Format Page
... identifying new interacting proteins and post-translational modifications will require technical and computational expertise, and biological insight. My graduate and post-graduate work in each of these areas helped advance the nascent proteomics field to its current state: pioneering investigations ...
... identifying new interacting proteins and post-translational modifications will require technical and computational expertise, and biological insight. My graduate and post-graduate work in each of these areas helped advance the nascent proteomics field to its current state: pioneering investigations ...
Effect of sol-gel encapsulation on the spectroscopic and
... using sol-gel processes has attracted the attention of several researchers over the past few years. These new composite materials incorporate the chemically selective functionality and reactivity of different biomolecules in solid state form and have led to the perspective of developing a new class ...
... using sol-gel processes has attracted the attention of several researchers over the past few years. These new composite materials incorporate the chemically selective functionality and reactivity of different biomolecules in solid state form and have led to the perspective of developing a new class ...
Proteins - Structure, folding and domains
... in order to study the folding pathway one needs to look at kinetics (e.g. trpfluorescence by stopped-flow rapid mixing) e.g. phi-value analysis of mutants (Ferhst & co-workers) Range from 0 to 1 (effect of mutation on denatured or folded state). ...
... in order to study the folding pathway one needs to look at kinetics (e.g. trpfluorescence by stopped-flow rapid mixing) e.g. phi-value analysis of mutants (Ferhst & co-workers) Range from 0 to 1 (effect of mutation on denatured or folded state). ...
Document
... to any organism • GO terms can be used to annotate gene products from any species, allowing comparison of information across species ...
... to any organism • GO terms can be used to annotate gene products from any species, allowing comparison of information across species ...