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701KB - NZQA
701KB - NZQA

... phenotypes _of various traits in genetically identical organisms. Armadillos are ideal animals to use in such research, because they are born as quadmplets derived from a single fertilised egg. This means that all four armadillo pups share the same genetic sequence. In a number of experiments carrie ...
Reassembled Biosynthetic Pathway for Large
Reassembled Biosynthetic Pathway for Large

... biosynthetic cycles from one strain to another for use in subsequent reactions. Instead of the E. coli only being modified by introduction of the glycosyltransferase genes, the superbug technology also overexpressed related enzymes essential for the regeneration of the sugar ± nucleotide conjugate r ...
MY FAVORITE PROTEIN Activity - Center for Biophysics and
MY FAVORITE PROTEIN Activity - Center for Biophysics and

... ribosomes already in this class. Proteins perform widely different tasks in our cells, but they are all made of the same 20 amino acids listed in your textbook. How is this possible? Because a protein’s structure – its 3dimensional form – and its function – how it performs its specific job - are ver ...
IDENTIFICATION OF A BACTERIO
IDENTIFICATION OF A BACTERIO

... 2. Radioactively labelled lysine, tyrosine and valine could not be ~corporated into peptide IV; the known sequence data show this peptide to lack these amino acids (as well as Ser, Phe, Asx). No other BrCN peptide lacks this combination of amino acids. 3. The amino acid analysis of purified peptide ...
Product PDF for Catalog Number: 103-M452
Product PDF for Catalog Number: 103-M452

... contains a pentaxinlike domain at its carboxyterminus that shares 2328% amino acid (aa) sequence similarity to Creactive protein (CRP) and serum amyloid P component (SAP), which belong to the short pentraxin subfamily. However, the Nterminal sequence of TSG 14 does not share aa sequence homology wit ...
fillable MS-Word version of the In Silico Kinase Match
fillable MS-Word version of the In Silico Kinase Match

... Provide ID name of your choice for this order for your own reference ...
Extended Methods
Extended Methods

... Calculation of atrophy and hypertrophy values in children muscle biopsies This statistical approach is particularly useful in detecting the presence of atrophy and hypertrophy that may not be apparent on routine inspection of a muscle biopsy. The method in adult muscle is based on the fact that near ...
Proteins containing unusual amino acid sequences
Proteins containing unusual amino acid sequences

... be imperfect repetitive sequence elements in collagen, compatible with the repeat distances recognized by fibre diffraction. Nevertheless, in general, proteins appeared to consist of non-repetitive information that seemed to be very specific, and which was being carefully edited by natural selection ...
Recombinant Protein L
Recombinant Protein L

... Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, qua ...
BR22, a 26 kDa thyroid transcription factor-1 associated protein
BR22, a 26 kDa thyroid transcription factor-1 associated protein

... HL63525 to Y-S. Yang, the Will Rogers Institute and the James M. Collins Center for Biomedical Research to J.C. Weissler. ...
Nickel affinity chromatography in Protein purification
Nickel affinity chromatography in Protein purification

... Iminodiacetic acid (IDA) both could be used to purify proteins with histidine molecules. NTA coordinates the Ni2+ with four valences and two valences are available for interaction with imidazole rings of histidine. ...
file1
file1

... - search for remote homologs using HMMs or profiles ...
Lecture 5: Major Nutrient Groups
Lecture 5: Major Nutrient Groups

... experimental conditions allow cannibalism, extrinsic sources of EAA (bacteria)  difficult to formulate reasonable diet and vary only one EAA ...
Solid Tumour Section Carcinoma with t(15;19) translocation Atlas of Genetics and Cytogenetics
Solid Tumour Section Carcinoma with t(15;19) translocation Atlas of Genetics and Cytogenetics

... translocation. In:World Health Organization classification of tumours. Pathology and genetics of tumours of the lung, thymus, pleura and heart. Travis WD, Brambilla E, MullerHermelink K, Harris CC, editors. Oxford University Press 2004. ...
Gene Expression and Basic Transformation
Gene Expression and Basic Transformation

... Viral Coat Protein ...
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... Closed monomers may be opened by mildly denaturing conditions or by mutations that destabilize the closed monomer. Open monomers may dimerize by domain swapping. The domain-swapped dimer has two C-interfaces identical to those in the closed monomer, however, each is formed between a domain from one ...
Nutr-4-Prot
Nutr-4-Prot

... Trypotophane (Try) Valine (Val) Histidine (His for Infant) Known as 1983 ...
Cloning and characterization of CmGPD1, the Candida magnoliae
Cloning and characterization of CmGPD1, the Candida magnoliae

... the pGEM-T Easy vector (Promega) to facilitate DNA sequencing. Escherichia coli DH5a and BL21 cells were used for plasmid preparation and expression host, respectively. Bacterial cells were grown at 37 1C in Luria–Bertani (LB) medium (0.5% yeast extract, 1% tryptone and 1% NaCl) supplemented with 10 ...
Supplementary Information (doc 662K)
Supplementary Information (doc 662K)

... ampicillin resistant cassette as the selection marker. Following plasmid amplification in DH5α cells, we prepared and isolated the plasmid. Human Nek2 plasmid was a generous gift from Prof. Andrew M. Fry, University of Leicester. The supplied plasmid contained Nek2 cDNA in a pGEM-3ZF (-) vector wit ...
Protein Structure
Protein Structure

... • Hard boiling an egg • Wiping the skin with alcohol swab for injection • Cooking food to destroy E. coli. • Heat used to cauterize blood vessels • Autoclave sterilizes instruments • Milk is heated to make yogurt ...
Amino Acids, Proteins, and Enzymes
Amino Acids, Proteins, and Enzymes

... • Wiping the skin with alcohol swab for injection • Cooking food to destroy E. coli. • Heat used to cauterize blood vessels • Autoclave sterilizes instruments • Milk is heated to make yogurt ...
On the Importance of Amino Acid Sequence and Spatial Proximity of
On the Importance of Amino Acid Sequence and Spatial Proximity of

... number of Cα-Cα interactions over the range 0–60 Å for specific residue types and fit this to simple sigmoids. The fits show no clear residue dependence at the range of interaction distances typically known to be relevant to protein structure and folding (0–10 Å). This is not surprising since the fi ...
Amino Acids, Proteins, and Enzymes
Amino Acids, Proteins, and Enzymes

... • Wiping the skin with alcohol swab for injection • Cooking food to destroy E. coli. • Heat used to cauterize blood vessels • Autoclave sterilizes instruments • Milk is heated to make yogurt ...
Amino Acids, Proteins, and Enzymes
Amino Acids, Proteins, and Enzymes

... • Wiping the skin with alcohol swab for injection • Cooking food to destroy E. coli. • Heat used to cauterize blood vessels • Autoclave sterilizes instruments • Milk is heated to make yogurt ...
Slide 1
Slide 1

...  Post-translational modifications (PTMs)  Covalent processing events that change the properties of a protein  proteolytic cleavage  addition of a modifying group to one or more amino acids  Determine its activity state, localization, turnover, interactions with other proteins  Mass spectrometr ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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