Single-stranded DNA-binding Proteins
... The combination of electrostatic, hydrogen-bonding and stacking interactions from proteins to ssDNA forms the basis for ssDNA binding and specificity. Unfortunately, dsDNA, dsRNA and single-stranded RNA (ssRNA) share many of these properties. How does an ssDNA-binding protein exclude these competing ...
... The combination of electrostatic, hydrogen-bonding and stacking interactions from proteins to ssDNA forms the basis for ssDNA binding and specificity. Unfortunately, dsDNA, dsRNA and single-stranded RNA (ssRNA) share many of these properties. How does an ssDNA-binding protein exclude these competing ...
Genes can be switched on and off by the protein CTCF
... I examined the regulator functions of CTCF. How strongly CTCF can bind to DNA depends on how the DNA is modified. Therefore it is important to investigate the conditions under which CTCF can function properly. I injected DNA into mouse embryos, using a special reporting system to see if CTCF was act ...
... I examined the regulator functions of CTCF. How strongly CTCF can bind to DNA depends on how the DNA is modified. Therefore it is important to investigate the conditions under which CTCF can function properly. I injected DNA into mouse embryos, using a special reporting system to see if CTCF was act ...
Mitochondrial transfer: Ethical, legal and social implications in assisted reproduction
... mitochondria, or the introduction of healthy donor mitochondria into affected oocytes. Blastomere, ooplasmic, pronuclear and spindle transfer have been explored. Ethical concerns relate to (a) the alteration of germ line genetics and (b) the dilemma of children inheriting DNA material from three ins ...
... mitochondria, or the introduction of healthy donor mitochondria into affected oocytes. Blastomere, ooplasmic, pronuclear and spindle transfer have been explored. Ethical concerns relate to (a) the alteration of germ line genetics and (b) the dilemma of children inheriting DNA material from three ins ...
11 Molecular Diagnostics
... difference compared to a reference standard that is present in at least 1–2% of a population. ...
... difference compared to a reference standard that is present in at least 1–2% of a population. ...
Application of Genetic Engineering
... • No variety is currently available for human consumption. Although golden rice was developed as a humanitarian tool, it has met with significant opposition from environmental and anti-globalization activists ...
... • No variety is currently available for human consumption. Although golden rice was developed as a humanitarian tool, it has met with significant opposition from environmental and anti-globalization activists ...
Supplementary Material Legends
... indicating cleavage sites of restriction enzymes and target region of probe used in methylation analysis by Southern blots. DNA was derived from individual plants that were siblings of the plants represented in Fig. 4b. A basic fragment containing the pNOS and part of the NPTII gene was released by ...
... indicating cleavage sites of restriction enzymes and target region of probe used in methylation analysis by Southern blots. DNA was derived from individual plants that were siblings of the plants represented in Fig. 4b. A basic fragment containing the pNOS and part of the NPTII gene was released by ...
Methicillin-resistant Staphylococcus aureus (MRSA) Real
... infections in humans. It may also be referred to as multiple-resistant Staphylococcus aureus or oxacillin-resistant Staphylococcus aureus (ORSA). MRSA is especially troublesome in hospital-associated (nosocomial) infections. In hospitals, patients with open wounds, invasive devices, and weakened imm ...
... infections in humans. It may also be referred to as multiple-resistant Staphylococcus aureus or oxacillin-resistant Staphylococcus aureus (ORSA). MRSA is especially troublesome in hospital-associated (nosocomial) infections. In hospitals, patients with open wounds, invasive devices, and weakened imm ...
Transformation Lab
... aureas (MRSA) infections.] Other plasmids code for an enzyme, toxin, or other protein that gives bacteria with that plasmid some survival advantage. In nature, bacteria may swap these beneficial plasmids from time to time. This process increases the variation between bacteria — variation that natura ...
... aureas (MRSA) infections.] Other plasmids code for an enzyme, toxin, or other protein that gives bacteria with that plasmid some survival advantage. In nature, bacteria may swap these beneficial plasmids from time to time. This process increases the variation between bacteria — variation that natura ...
Slide 1
... S. pneumoniae with a central founder ST199 and 12 linked SLVs; two of the SLVs have diversified to produce DLVs. eBURST, unlike cluster diagrams, trees, or dendograms, uses a simple model of bacterial evolution in which an ancestral (or founding) genotype increases in frequency in the population and ...
... S. pneumoniae with a central founder ST199 and 12 linked SLVs; two of the SLVs have diversified to produce DLVs. eBURST, unlike cluster diagrams, trees, or dendograms, uses a simple model of bacterial evolution in which an ancestral (or founding) genotype increases in frequency in the population and ...
tools and techniques
... The greek letter “mu” is used to represent micro (μ) Let’s PRACTICE Work through the second page of the packet “DNA Fingerprinting – Practice Worksheet” ...
... The greek letter “mu” is used to represent micro (μ) Let’s PRACTICE Work through the second page of the packet “DNA Fingerprinting – Practice Worksheet” ...
Sequence Analysis of the y-Globin Gene Locus from
... conversion of the sequences of a normal y-globin gene to that The sequence of the '7-globin gene from -383 to +2,209 of another normal y-globin gene cannot explain the phenobp (relative to the cap site) was identical to 'y-globin from the previously reported chromosome A sequence f r a m e ~ o r k , ...
... conversion of the sequences of a normal y-globin gene to that The sequence of the '7-globin gene from -383 to +2,209 of another normal y-globin gene cannot explain the phenobp (relative to the cap site) was identical to 'y-globin from the previously reported chromosome A sequence f r a m e ~ o r k , ...
letters Solution structure of the DNA-binding domain of MafG
... DNA-binding motifs, is found in many eukaryotic and prokaryotic transcriptional regulatory proteins. The second helix of the motif usually binds to the major groove of DNA and, therefore, is mainly involved in recognition and interaction with specific DNA sequences10. MafG(1–76) contains a similar H ...
... DNA-binding motifs, is found in many eukaryotic and prokaryotic transcriptional regulatory proteins. The second helix of the motif usually binds to the major groove of DNA and, therefore, is mainly involved in recognition and interaction with specific DNA sequences10. MafG(1–76) contains a similar H ...
biology syllabus - prakashamarasooriya
... Outline the role of condensation and hydrolysis in the relationships between monosaccharides, disaccharides and polysaccharides; between fatty acids, glycerol and triglycerides; and between amino acids and polypeptides. ...
... Outline the role of condensation and hydrolysis in the relationships between monosaccharides, disaccharides and polysaccharides; between fatty acids, glycerol and triglycerides; and between amino acids and polypeptides. ...
Structural insights into the first incision reaction
... Tyr 19, Tyr 29, Lys 32, Arg 39, Glu 76 and Asn 88. These strictly conserved amino acids are surrounded by a number of highly conserved residues: Tyr 43, Phe 73 and Ile 80. The conserved residues form a shallow, concave surface with dimensions of 16 Å 15 Å and 5 Å deep, which could easily accomm ...
... Tyr 19, Tyr 29, Lys 32, Arg 39, Glu 76 and Asn 88. These strictly conserved amino acids are surrounded by a number of highly conserved residues: Tyr 43, Phe 73 and Ile 80. The conserved residues form a shallow, concave surface with dimensions of 16 Å 15 Å and 5 Å deep, which could easily accomm ...
Large Scale SNP Scanning on Human Chromosome Y and DNA
... Many genetic diseases have yet to be located on the human genome for reasons that include their multiple loci and incomplete penetration. To pinpoint these loci in terms of particular regions of the chromosomes, association studies, which compare allele frequency between affected individuals (proban ...
... Many genetic diseases have yet to be located on the human genome for reasons that include their multiple loci and incomplete penetration. To pinpoint these loci in terms of particular regions of the chromosomes, association studies, which compare allele frequency between affected individuals (proban ...
FANCE Antibody
... (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersen ...
... (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersen ...
Biotechnology
... a. For identification of a specific gene in a DNA extraction by hybridization to a DNA probe. b. For identification of a specific gene by hybridization to a DNA probe within live cells that have had their DNA denatured by heat. c. For identification of an mRNA within an RNA extraction by hybridizat ...
... a. For identification of a specific gene in a DNA extraction by hybridization to a DNA probe. b. For identification of a specific gene by hybridization to a DNA probe within live cells that have had their DNA denatured by heat. c. For identification of an mRNA within an RNA extraction by hybridizat ...
Different physical delivery systems: An important approach for
... cytotoxicity. Compared to electroporation, microinjection requires low protein amounts. This is efficient for transferring recombinant proteins and synthesized peptides with high cost. In contrast with chemical transfection (e.g., liposomes) and viral infection, microinjection of the cDNAs into cell ...
... cytotoxicity. Compared to electroporation, microinjection requires low protein amounts. This is efficient for transferring recombinant proteins and synthesized peptides with high cost. In contrast with chemical transfection (e.g., liposomes) and viral infection, microinjection of the cDNAs into cell ...
Chromosome mapping of the sweet potato little leaf
... Northern Territory University, Faculty of Science, Darwin, Northern Territory 0909, Australia ...
... Northern Territory University, Faculty of Science, Darwin, Northern Territory 0909, Australia ...
RiboPrinter® microbial characterization system
... consistency. The RiboPrinter system provides the speed, accuracy, reproducibility and confidence never before possible. From start to finish, the RiboPrinter system process is automated, simplifying operator training and minimizing errors due to technique. Loading and operating the characterization ...
... consistency. The RiboPrinter system provides the speed, accuracy, reproducibility and confidence never before possible. From start to finish, the RiboPrinter system process is automated, simplifying operator training and minimizing errors due to technique. Loading and operating the characterization ...
Bacterial
... you have prepared these plates they should ideally be used within 48 hours. To achieve good results, it is essential that students scrape a reasonable mass of cells from the stock plate, taking care not to scrape any agar from the plate. [If you keep the cultures for five days, you will still obtain ...
... you have prepared these plates they should ideally be used within 48 hours. To achieve good results, it is essential that students scrape a reasonable mass of cells from the stock plate, taking care not to scrape any agar from the plate. [If you keep the cultures for five days, you will still obtain ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.