genes, pseudogenes, deletions, insertion elements and DNA islands
... genome projects for homologies with the opcA sequence. The < 4 kb region from aroG to comEA containing CopcB was also sequenced from chromosomal PCR products from strains Z2491, FA1090 and FAM18 (Fig. 2). The map locations of the opcA and CopcB regions were determined by hybridizing opcA and CopcB p ...
... genome projects for homologies with the opcA sequence. The < 4 kb region from aroG to comEA containing CopcB was also sequenced from chromosomal PCR products from strains Z2491, FA1090 and FAM18 (Fig. 2). The map locations of the opcA and CopcB regions were determined by hybridizing opcA and CopcB p ...
Putrescine oxidase of Micrococcus rubens : primary
... Hybridization was then done with the 32P-labelledprobes at 42 "C for 16 h in the same buffer. The nitrocellulose filters were washed three times in 2 x SSC (1 x SSC is 0.15 M-NaCI, 0.015 M-sodium citrate) containing 0.1 YO SDS. A distinct 4.4 kb band hybridizing to three probes was detected. From an ...
... Hybridization was then done with the 32P-labelledprobes at 42 "C for 16 h in the same buffer. The nitrocellulose filters were washed three times in 2 x SSC (1 x SSC is 0.15 M-NaCI, 0.015 M-sodium citrate) containing 0.1 YO SDS. A distinct 4.4 kb band hybridizing to three probes was detected. From an ...
Mechanism of DNA Alkylation by Duocarmycin SA
... The duocarmycin payload is released from the antibody through protease cleavage of the dipeptide linker, and is then converted from its pro-drug form to the cyclopropane via carboxylesterase cleavage of the carbamate. ...
... The duocarmycin payload is released from the antibody through protease cleavage of the dipeptide linker, and is then converted from its pro-drug form to the cyclopropane via carboxylesterase cleavage of the carbamate. ...
Automated genomic DNA purification of 6 different marine
... The PCR reaction conditions cannot be described in this Application Note because the data are not published yet. PCR was performed with an Eppendorf Mastercycler ® gradient pro S instrument with the Promega GoTaq® Flexi DNA polymerase kit and specifics primers for each species. Determination of yiel ...
... The PCR reaction conditions cannot be described in this Application Note because the data are not published yet. PCR was performed with an Eppendorf Mastercycler ® gradient pro S instrument with the Promega GoTaq® Flexi DNA polymerase kit and specifics primers for each species. Determination of yiel ...
Mutation, Repair, and Recombination
... reversion at the original mutant site may have occurred. Prototroph B: half the progeny are parental prototrophs, and the remaining prototrophs, 28 percent, are the result of the new mutation. Notice that 28 percent is approximately equal to the 22 percent auxotrophs. The suggestion is that an unlin ...
... reversion at the original mutant site may have occurred. Prototroph B: half the progeny are parental prototrophs, and the remaining prototrophs, 28 percent, are the result of the new mutation. Notice that 28 percent is approximately equal to the 22 percent auxotrophs. The suggestion is that an unlin ...
Data for two plasmid isolation techniques, the rapid alkaline extraction... Nucleic Acids Res. 7: 1513-1523) and the rapid boiling technique...
... Neurospora transformants were observed with both types of plasmid DNA minipreps (Table I). The alkaline extraction preparations proved to be the most effective for transformation. However, in all cases the number of transformants obtained was substantially lower (one-fifth to one-twentieth) than the ...
... Neurospora transformants were observed with both types of plasmid DNA minipreps (Table I). The alkaline extraction preparations proved to be the most effective for transformation. However, in all cases the number of transformants obtained was substantially lower (one-fifth to one-twentieth) than the ...
SafeView - NBS Biologicals
... This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse sperma ...
... This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse sperma ...
Biol120 Mock Final Examination
... 41. C. Botulinum is a rod-shaped bacterium that produces a toxin that causes botulism in humans. Most botulism cases occur when food that was improperly canned is eaten. These bacteria can only grow in the oxygen-free environment of a sealed can, and the moist food provides the bacteria with a ready ...
... 41. C. Botulinum is a rod-shaped bacterium that produces a toxin that causes botulism in humans. Most botulism cases occur when food that was improperly canned is eaten. These bacteria can only grow in the oxygen-free environment of a sealed can, and the moist food provides the bacteria with a ready ...
mRNA
... as a template upon which a complementary strand of RNA is assembled from nucleotides • In contrast with DNA replication, only part of one DNA strand, not the whole molecule, is used as a template for transcription © Cengage Learning 2015 ...
... as a template upon which a complementary strand of RNA is assembled from nucleotides • In contrast with DNA replication, only part of one DNA strand, not the whole molecule, is used as a template for transcription © Cengage Learning 2015 ...
Adenine - One of the four bases in DNA tha make up the letters
... Cellulose - A sugar-rich compound of cell walls of plants and some protoctists CentiMorgan (cM) - (1) A measure of genetic distance that tells how far apart two genes are. Generally one centimorgan equals about 1 million base pairs. (2) A unit used to express distances on a genetic map. In genetic m ...
... Cellulose - A sugar-rich compound of cell walls of plants and some protoctists CentiMorgan (cM) - (1) A measure of genetic distance that tells how far apart two genes are. Generally one centimorgan equals about 1 million base pairs. (2) A unit used to express distances on a genetic map. In genetic m ...
Chapter 14
... Ribosomes have two subunits, large and small, held together noncovalently. In eukaryotes, the large subunit has three different molecules of ribosomal RNA (rRNA) and 49 different proteins in a precise pattern. The small subunit has one rRNA and ...
... Ribosomes have two subunits, large and small, held together noncovalently. In eukaryotes, the large subunit has three different molecules of ribosomal RNA (rRNA) and 49 different proteins in a precise pattern. The small subunit has one rRNA and ...
Carcinomas with DNA Mismatch Repair Deficiency
... The DNA mismatch repair proteins are ubiquitously expressed in normal human tissues, particularly proliferating tissues, and nuclear expression in crypt epithelium and lymphocytes serves as an internal positive control for stain quality. In the setting of HNPCC, most hereditary and second-hit tumor ...
... The DNA mismatch repair proteins are ubiquitously expressed in normal human tissues, particularly proliferating tissues, and nuclear expression in crypt epithelium and lymphocytes serves as an internal positive control for stain quality. In the setting of HNPCC, most hereditary and second-hit tumor ...
Compiling DNA strand displacement reactions using a functional
... Previous work has shown that synthetic DNA circuits can be used to implement computational systems including digital logic circuits [1], neural networks [2] and gameplaying automata [3]. In this setting, DNA is used both as an information carrier and as an engineering material, simultaneously. Furth ...
... Previous work has shown that synthetic DNA circuits can be used to implement computational systems including digital logic circuits [1], neural networks [2] and gameplaying automata [3]. In this setting, DNA is used both as an information carrier and as an engineering material, simultaneously. Furth ...
Genetics - Brookwood High School
... A. When humans make a change in an organism’s DNA code. B. In recombinant DNA , genes from one species can be inserted into another. C. Is frequently used in many of the foods we eat. ...
... A. When humans make a change in an organism’s DNA code. B. In recombinant DNA , genes from one species can be inserted into another. C. Is frequently used in many of the foods we eat. ...
2010 HSC Exam Paper - Biology
... population? (A) It results in many genetically identical individuals. (B) It makes certain alleles more common in a population. (C) It decreases the number of chromosomes in some individuals. (D) It ensures that only the genetic composition of the males is altered. ...
... population? (A) It results in many genetically identical individuals. (B) It makes certain alleles more common in a population. (C) It decreases the number of chromosomes in some individuals. (D) It ensures that only the genetic composition of the males is altered. ...
Sterile, 24-well tissue culture plates are filled with melted minimal ... 1.0 ml per well using a repeating syringe. After the...
... were recessive to their respective wild type alleles, but complementation of the amino acid analogue sensitive phenotype was not observed in heterocaryons carrying mutant alleles simultaneously. These findings suggest that cpc-1, mts(MN9) as well as mts(MNl) belong to the same complementation group. ...
... were recessive to their respective wild type alleles, but complementation of the amino acid analogue sensitive phenotype was not observed in heterocaryons carrying mutant alleles simultaneously. These findings suggest that cpc-1, mts(MN9) as well as mts(MNl) belong to the same complementation group. ...
Multiple Sequence Alignment
... Multiple Sequence Alignment: Background The purpose of this tutorial is to describe several commonly encountered Multiple Sequence Alignment (MSA) format types, namely the (1) Clustal, (2) FASTA, and (3) Phylip MSA formats. MSAs are alignments of three or more DNA, RNA or protein sequences. Usually ...
... Multiple Sequence Alignment: Background The purpose of this tutorial is to describe several commonly encountered Multiple Sequence Alignment (MSA) format types, namely the (1) Clustal, (2) FASTA, and (3) Phylip MSA formats. MSAs are alignments of three or more DNA, RNA or protein sequences. Usually ...
Characterization of the soil microbial community associated with the
... bacterial counts for each sampling event was determined using the pour plate method. This method included diluting 1 g of soil from each homogenized sample in 99 ml of sterile saline solution (0.9% (w v1) NaCl) and mixed thoroughly. Standard serial dilutions followed and a 1-ml aliquot of each dilu ...
... bacterial counts for each sampling event was determined using the pour plate method. This method included diluting 1 g of soil from each homogenized sample in 99 ml of sterile saline solution (0.9% (w v1) NaCl) and mixed thoroughly. Standard serial dilutions followed and a 1-ml aliquot of each dilu ...
Chapter 2. Structures of Nucleic Acids
... DNA and RNA are both nucleic acids, which are the polymeric acids isolated from the nucleus of cells. DNA and RNA can be represented as simple strings of letters, where each letter corresponds to a particular nucleotide, the monomeric component of the nucleic acid polymers. Although this conveys alm ...
... DNA and RNA are both nucleic acids, which are the polymeric acids isolated from the nucleus of cells. DNA and RNA can be represented as simple strings of letters, where each letter corresponds to a particular nucleotide, the monomeric component of the nucleic acid polymers. Although this conveys alm ...
HIGH FREQUENCY GENE TARGETING USING INSERTIONAL
... clones for introduction of specific mutations by the ‘hit and run’ procedure (14). The vector pHRNTF508 (Fig. 1B) was used to target the Cftr gene and 2 out of 53 (3.8%) clones obtained were identified as correctly targeted by Southern blot analysis. This frequency of homologous recombination repre ...
... clones for introduction of specific mutations by the ‘hit and run’ procedure (14). The vector pHRNTF508 (Fig. 1B) was used to target the Cftr gene and 2 out of 53 (3.8%) clones obtained were identified as correctly targeted by Southern blot analysis. This frequency of homologous recombination repre ...
Isolation of a gene encoding a novel chloroplast protein by T
... 156 offspring tested were hygromycin resistant but split to 79 green and 77 pale. The 1:1 segregation ratio of green and pale Fl progenies confirmed (P = 0.86) the allelism and the absence of complementation between cs and ch-42 mutations, permitting cs to be assigned to position 39.4-4 of ch-42 on ...
... 156 offspring tested were hygromycin resistant but split to 79 green and 77 pale. The 1:1 segregation ratio of green and pale Fl progenies confirmed (P = 0.86) the allelism and the absence of complementation between cs and ch-42 mutations, permitting cs to be assigned to position 39.4-4 of ch-42 on ...
- Wiley Online Library
... of the perturbations in the population(s) must be determined following physical separation of cells into two or more populations. Previously, this was often performed using hybridization techniques such as microarrays, but, more recently, massively parallel sequencing has become the method of choice ...
... of the perturbations in the population(s) must be determined following physical separation of cells into two or more populations. Previously, this was often performed using hybridization techniques such as microarrays, but, more recently, massively parallel sequencing has become the method of choice ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.