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Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

Chapter 8 DNA and RNA
Chapter 8 DNA and RNA

OCR Biology AS and A2 GCE specifications for
OCR Biology AS and A2 GCE specifications for

... Describe, with the aid of diagrams, the way in which a nucleotide sequence codes for the amino acid sequence in a polypeptide; Describe, with the aid of diagrams, how the sequence of nucleotides within a gene is used to construct a polypeptide, including the roles of messenger RNA, transfer RNA and ...
Molecular Biology -
Molecular Biology -

...  amino acid sequence in a polypeptide which folds into a ____________ translation  structure and function of the protein (e.g. normal hemoglobin vs. sickle cell hemoglobin)  person's characteristics or traits (e.g. normal health vs. sickle cell anemia) 2. The double helix structure of DNA, transc ...
Part 5 Intro to Genetics:
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... replaced by a normal gene. Scientists have attempted gene therapy with the use of viruses because of their ability to enter a cell’s DNA. First the virus particles are modified so that they cannot cause disease. Then a fragment of DNA containing the replacement gene is spliced onto the viral DNA. Th ...
English Version
English Version

... (2) Regulation of transcriptional initiation is the key step in regulation of gene expression. (3) Induction and repression are the basic models of prokaryotic gene expression. 2. Bacterial operons. (1) Concept of operon. (2) Lac operon. (3) Other important operons. 3. Regulation of gene expression ...
DNA: the Molecule of Heredity
DNA: the Molecule of Heredity

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Bacterial culture Microbiological cultures can be grown in petri
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Nucleic Acids and Protein Synthesis
Nucleic Acids and Protein Synthesis

Genetic Engineering - Valhalla High School
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Molecular Genetics
Molecular Genetics

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... DNA Shearing on Covaris M220 with regular PCR tubes Introduction The Covaris microTUBE, such as the Screw-Cap 6x16mm (#520096, left in the figure below), is specifically designed and engineered for compatibility with Focusedultrasonicator utilizing AFA technology. The microTUBE are made of a special ...
Bacteria & Archaea Prokaryote
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... • Prokaryote: no nucleus or organelles – Chromosome & plasmids float freely in cytoplasm • Ribosomes: create proteins • Flagella: used in movement • Pili: act as anchors • Capsule: outer coating • Endospore: “cocoon” that forms around DNA to protect in harsh times ...
Biogenetic Engineering & Manipulating Genes
Biogenetic Engineering & Manipulating Genes

... Manipulating genes and sequencing DNA has become one of the greatest achievements in the last 50 years -First complete genome sequenced was a bacteria (1995) -Human Genome project (1990-2003) ...
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DNA damage and repair

... Cells cannot function if DNA damage corrupts the integrity and accessibility of essential information in the genome (but cells remain superficially functional when so-called "non-essential" genes are missing or damaged). ...
Lab #7 Exoenzymes, Differential and Selective Media
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... hydrolyzing starch produce the enzyme amylase. This enzymatic hydrolysis forms shorter polysaccharides called dextrins, which ultimately can be hydrolyzed to yield disaccharides and monosaccharides. These end products are transported into the cell and used in biosynthetic reactions (e.g., formation ...
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... Making Copies In order to study genes, biologists often need to make many copies of a particular gene. Like a photocopy machine stuck on "print," a technique known as polymerase chain reaction (PCR) allows biologists to do exactly that. The figure below shows how PCR works. The idea behind PCR is su ...
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... Making Copies In order to study genes, biologists often need to make many copies of a particular gene. Like a photocopy machine stuck on "print," a technique known as polymerase chain reaction (PCR) allows biologists to do exactly that. The figure below shows how PCR works. The idea behind PCR is su ...
Extraction of DNA from an Onion
Extraction of DNA from an Onion

... Molecular biologists and biochemists are involved with research in finding out as much as possible about the DNA in plants and animals. Although DNA was discovered in the 1950’s, there still remains a lot to be known about it, especially how it is used to determine the physical traits that we all ha ...
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... bacteria expressing the GFP gene? E. coli with the pGLO plasmid could grow on all of these, but would only glow on the plate with arabinose added because this sugar turns on the ara gene which also results in the expression of the “glowing” gene. 3.A.1.e. Genetic engineering techniques can manipulat ...
Principles of BIOCHEMISTRY
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... • Cloning vectors can be: plasmids, bacteriophages, viruses, small artificial chromosomes • Some vectors can be replicated autonomously in a host cell, other vectors can be integrated into the host chromosome • Vectors have at least one unique cloning site: a sequence cut by a restriction endonuclea ...
DNA - Madison Public Schools
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... The sex of a DNA contributor can be determined by looking at the amelogenin gene. The gene is 6 bases shorter on the X chromosome than it is on the Y chromosome.  Y-STR’s – STR’s found on the Y chromosome; useful when there is a mix of DNA from more than one ...
E. coli
E. coli

Biology - secondary
Biology - secondary

... • What is the most important function of Chlorophyll? • Which phase of photosynthesis takes place in either the presence or absence of darkness • Which is the most directly involved in photosynthesis-Chlorophyll a or Chlorophyll b? • ________- The process of obtaining energy from inorganic materials ...
Transformation after ligation
Transformation after ligation

... In this laboratory, you will use bacteria to learn how to perform a transformation which is the process of introducing DNA into bacterial cells. We will be introducing a plasmid as the foreign DNA. You will be given a plasmid that has a gene for Ampicillin resistance. If the bacteria take up the pla ...
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Transformation (genetics)



In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".
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