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BIOLOGY 12 Single Trait Inheritance and Test Cross Single Trait Inheritance  solving problems that involve just one gene with two alleles  use a 2x2 Punnett Square  have up to 4 possible outcomes Test Cross  used to determine the genotype of an individual  either homozygous dominant or heterozygous Test Cross  example:    a black sheep is not preferred because the wool is brittle and difficult to dye black wool is recessive to ensure you will have no black sheep, you must have at least one homozygous dominant parent Test Cross  use a test cross to determine the genotype of an individual with a dominant phenotype  cross homozygous recessive with the unknown individual Test Cross Test Cross  if any of the offspring show the recessive trait, then the parent must be heterozygous Practice  for Labrador retrievers, black fur colour is dominant over yellow  what would be the genotype of a homozygous black dog? a heterozygous black dog?  a yellow female mates with a male with an unknown genotype, if the pups all have black fur, what is the genotype and phenotype of the male? explain. Practice  for Dalmatian dogs, the spotted condition is dominant over non-spotted  using a punnett square, show the results of a cross between two heterozygous parents  a heterozygous spotted female Dalmatian dog mates with an unknown father. from the appearance of the pups, the owner concludes the male was a Dalmatian. the owner notes that the female has 3 spotted and 3 non-spotted pups. what are the genotype and phenotype of the unknown mate?