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Cell Structure & Function http://koning.ecsu.ctstateu.edu/cell/cell.html Discovery of the Cell • Mid-1600s scientists began using microscopes to observe living things • 1665, ______________looked at cork with early compound microscope • Thousands of tiny empty chambers called ______________ • 1665(-ish) Anton van Leeuwenhoek used single-lens scope to look at ______________ • What did he see? The Cell Theory • More observations confirmed that CELLS were the ___________________ • 1838, Matthias Schleiden-all plants made of cells (pg. 170, Fig. 7-2) • 1839, Theodore Schwann, all animals made of cells • 1855, Rudolf Virchow, new cells produced only from division of existing cells Discoveries Summarized in The Cell Theory 1. All living things are made up of __________ 2. Cells are the smallest working units of all living things. 3. All cells come from ___________ through process of ________________. Definition of Cell A cell is the smallest unit that is capable of performing life functions. Exploring the Cell • Fluorescent labels • Light microscopy • Confocal light microscopy • High-resolution video technology • TEM • SEM • Scanning probe microscope **See page 172 for some nice images! Examples of Cells amoeba Plant Bacteria Nerve cell Red Blood cell Two Types of Cells Prokaryotic • Do not have structures surrounded by membranes • Few internal structures • One-celled organisms, EX: bacteria • No nucleus for DNA http://library.thinkquest.org/C004535/prokaryotic_cells.html Eukaryotic • Contain organelles surrounded by membranes • Most living organisms • DNA contained in nucleus Plant http://library.thinkquest.org/C004535/eukaryotic_cells.html Animal “Typical” Animal Cell http://web.jjay.cuny.edu/~acarpi/NSC/images/cell.gif “Typical” Plant Cell http://waynesword.palomar.edu/images/plant3.gif 7.2 Eukaryotic Cell Structure • The eukaryotic cell is like a factory • Many structures in the cell act like specialized organs…ORGANELLES • Eukaryotic cell divided into 2 parts: 1. nucleus 2. cytoplasm (outside the nucleus) They work together Nucleus • Control center of cell, “main office, boss” • Contains the cell’s DNA and instructions for making proteins and other important molecules • Surrounded by nuclear envelope or membrane (made of 2 membranes) • Separates it from cytoplasm • Nuclear pores so material can move in and out of nucleus (ex. Proteins, RNA) Nucleus (cont.) • Chromatin- granular material made of DNA bound to protein • Most of time it is spread throughout nucleus • During division it condenses and forms CHROMOSOMES (threadlike, contain genetic info that’s passed to next generation) Nucleolus • Inside nucleus • Contains RNA to build proteins • ribosome assembly begins here http://library.thinkquest.org/12413/structures.html Ribosomes • **PROTEINS are put together on the ribosomes (cell is “protein-making machine”) Protein synthesis • Small particles of RNA and protein found throughout the cytoplasm Endoplasmic Reticulum, ER • Extension of nucleus • Moves materials around in cell (transportation) • Rough type shown: ribosomes on surface • Site of protein synthesis ER (cont.) • Proteins and other materials exported from cell are assembled here • New proteins leave the ribosomes • Some are inserted into the rough ER and are chemically modified Smooth ER • No ribosomes on surface • Can hold collections of enzymes that carry out certain jobs: -synthesis of membrane lipids -detox of drugs **liver cells contain a lot of smooth ER Golgi Apparatus • Protein “packaging plant” • Material traveling on ER is modified, sorted, packaged and labeled for intra- and extracellular transportation Golgi Apparatus (cont.) “customization shop”, finishing touches are put on proteins before they are “shipped” to other places in or outside the cell Lysosomes are produced here Lysosomes • “Digestive plant” for proteins, lipids, and carbohydrates • Filled with enzymes • Transports undigested material to cell membrane for removal • Cell breaks down if lysosome explodes Vacuoles • Sac-like structures, storage area • Store water, salts, proteins, CHOs • Plants can have one large Central Vacuole filled with liquid • Allows plants to support leaves, flowers • Contractile vacuole in paramecium pumps water out of cell (homeostasis) Mitochondria • Convert the chemical energy stored in food into compounds that the cell can use “Powerhouse of the cell” -Enclosed by two membranes —outer and inner membrane • The inner membrane folds over many times (cristae) Where chem. Rxns. Happen • In humans, mitochondria come from Mom Chloroplasts • Found in plant cells • Capture the energy from sunlight and convert it into chemical energy during photosynthesis • “Solar power plants” • Surrounded by two membranes • Inside are large stacks of other membranes, which contain the green pigment chlorophyll. Organelle DNA Chloroplasts and mitochondria contain their own small DNA molecules • Lynn Margulis, an American biologist, suggested mitochondria and chloroplasts are the descendants of ancient prokaryotes. • Cytoskeleton • Network of protein filaments • Structure , support, maintain shape of cell • Involved in movement Microfilaments Microtubules Microfilaments • Threadlike • Made of actin • Tough, flexible framework that gives support • movement Microtubules Hollow, made of proteins called tubulins Help build cilia and flagella allowing cells to move Form centrioles Centrioles • Found only in animal cells • Set up the framework that moves the chromosomes to opposite sides of the cell during cell division. Surrounding the Cell Cell Membrane • Outer membrane of cell that controls movement in and out of cell • nearly all cell membranes made of double-layered sheet called a lipid bilayer • flexible structure and strong barrier between the cell and its surroundings. Cell Membrane (cont.) • two layers of lipids • protein molecules embedded in the lipid bilayer • Carbohydrate molecules attached to many of the proteins • Structure of cell membrane Diffusion • Particles move from an area of high concentration to an area of low concentration • Diffusion depends on random particle movements so substances diffuse across membranes without using energy • Equilibrium- when the concentration of the solute is the same throughout a system Functions of proteins and CHOs • Some of the proteins form channels and pumps that help to move material across the cell membrane • Many of the CHOs act like chemical identification cards and allow individual cells to identify each another Cell Wall • provide support and protection for the cell. • plants, algae, fungi, and many prokaryotes • outside the cell membrane • porous enough to allow water, oxygen, carbon dioxide, and certain other substances to pass through easily. http://library.thinkquest.org/12413/structures.html Cell Wall (cont.) • made from fibers of carbohydrate and protein • Plant cell walls are composed mostly of cellulose (a tough CHO fiber) • Wood and paper (cellulose) living cells exist in a liquid environment One of the most important functions of the cell membrane- regulate the movement of dissolved molecules from the liquid on one side of the membrane to the liquid on the other side • Cytoplasm contains a solution of many different substances in water • A solution is a mixture of two or more substances • Solute dissolved in solvent • The concentration of a solution is the mass of solute in a given volume of solution, or mass/volume • Ex. 12 g of salt dissolved in 3 liters of water Osmosis • If a substance can diffuse across a membrane, the membrane is permeable • Impermeable to substances that cannot cross it • Most biological membranes are selectively permeable • Osmosis is the diffusion of water through a selectively permeable membrane Osmotic Pressure • Cells need a way to balance the intake and loss of water • Osmosis exerts a pressure (osmotic pressure) on the hypertonic side of a selectively permeable membrane • Osmotic pressure can cause serious problems for a cell. • cell contains salts, sugars, proteins, and other molecules making it hypertonic to fresh water • Osmotic pressure should produce a net movement of water into a typical cell that is surrounded by fresh water • If that happens, volume of a cell will increase until the cell becomes swollen • Eventually, the cell may burst like an overinflated balloon • Most cells in large organisms do not come in contact with fresh water • Cells are in fluids like blood that are isotonic • Isotonic fluids have concentrations of dissolved materials roughly equal to those in the cells themselves • plant cells and bacteria do come into contact with fresh water but have tough cell walls • Cell walls prevent the cells from expanding • Increased osmotic pressure makes the cells more likely to experience injuries to their cell walls Facilitated Diffusion • cell membranes have protein channels • make it easy for certain molecules to cross the membrane • Red blood cells have a cell membrane protein with an internal channel that allows only glucose to pass through it • It facilitates, or helps, the diffusion of glucose across the membrane -facilitated diffusion Active Transport • Requires energy to move cells against a concentration gradient • Small molecules, ions can be moved using transport proteins or pumps in the membrane • Larger molecules can be moved across membrane by endocytosis and exocytosis Molecular Transport • Ca, K, Na moved across membrane by proteins that act like pumps Endo/Exocytosis • Larger molecules, solid clumps of material • Endocytosis-materials taken into the cell by infoldings, pockets, of cell membrane Phagocytosis • “cell eating” • Extensions of cytoplasm surround particle and package it in a food vacuole • Cell engulfs it • Ex. Amoebas • Uses a lot of energy Pinocytosis • Cells take up liquid from environment • Pockets form along cell membrane and fill with liquid • Then pinch off and form vacuoles in the cell Exocytosis • Release material from the cell • Membrane of vacuole surrounding the material fuses with the cell membrane and forces contents out of cell • Ex. Contractile vacuole removes water 7.4 Diversity of Cellular Life • Multicellular-organisms made up of many cells • all multicellular organisms depend on communication and cooperation among specialized cells • Cells within an organism can develop in different ways to perform different tasks in a process called cell specialization Specialized Animal Cells • Red blood cells-specialized to transport oxygen • contain a protein that binds to oxygen in the lungs and transports the oxygen throughout the body where it is released • Cells specialized to produce proteins are found in the pancreas • Pancreas- a gland that produces enzymes that make it possible to digest food • Pancreatic cells are packed with ribosomes and rough ER • Pancreatic cells also have a well-developed Golgi apparatus and clusters of storage vacuoles loaded with enzymes • human ability to move is due to specialized structures of muscle cells • They generate force by using an overdeveloped cytoskeleton • Skeletal muscle cells are packed with fibers arranged in a tight, regular pattern • The fibers are actin microfilaments and a cytoskeletal protein called myosin Specialized Plant Cells • exchange carbon dioxide, oxygen, water vapor, and other gases through tiny openingsstomata (on the undersides of leaves) • Specialized cells called guard cells regulate the exchange • Guard cells monitor the plant's internal conditions and change their shape according to the conditions • When the plant can benefit from gas exchange, the stomata open • The stomata close tightly when the plant's internal conditions change Levels of Organization • in a multicellular organism individual cells tissues organs organ systems. • Similar cells are grouped into units called tissues • Tissue is a group of similar cells that perform a certain function • The collection of cells that produce digestive enzymes in the pancreas makes up one kind of tissue • Most animals have four main types of tissue: muscle, epithelial, nervous, and connective tissue • Many groups of tissues work together as an organ • Each muscle in your body is an individual organ • Within a muscle there are nerve tissues and connective tissues • Each type of tissue performs an important task to help the organ function • A group of organs that work together to perform a specific function is called an organ system • Example: Digestive system • Muscle cells make up smooth muscle tissue and along with other tissues makes up the stomach (organ). The stomach is part of digestive system (organ system) pg. 193 Inside the Cell Chromosomes • In nucleus • Made of DNA • Contain instructions for traits & characteristics http://library.thinkquest.org/12413/structures.html Cytoplasm • Gel-like mixture • Surrounded by cell membrane • Contains hereditary material Mitochondria • Produces energy through chemical reactions – breaking down fats & carbohydrates • Controls level of water and other materials in cell • Recycles and decomposes proteins, fats, and carbohydrates http://library.thinkquest.org/12413/structures.html Vacuoles • Membrane-bound sacs for storage, digestion, and waste removal • Contains water solution • Help plants maintain shape http://library.thinkquest.org/12413/structures.html Chloroplast • Usually found in plant cells • Contains green chlorophyll • Where photosynthesis takes place http://library.thinkquest.org/12413/structures.html