Download Movement Across Cell - Mrs. Rowland`s Science Classes

The Cell Membrane
AP Biology
2007-2008
Membrane is a collage of proteins & other molecules
embedded in the fluid matrix of the lipid bilayer
Glycoprotein
Extracellular fluid
Glycolipid
Phospholipids
Cholesterol
Peripheral
protein
AP Biology
Transmembrane
proteins
Cytoplasm
Filaments of
cytoskeleton
1972, S.J. Singer & G. Nicolson proposed Fluid Mosaic Model
Phospholipids
 Phosphate head

“attracted to water”
hydrophilic
 Fatty acid tails

Phosphate
hydrophobic
 Arranged as a bilayer
Fatty acid
“repelled by water”
Aaaah,
one of those
structure–function
examples
AP Biology
Cell membrane defines cell
 Cell membrane separates living cell from
aqueous environment

thin barrier = 8nm thick
 Controls traffic in & out of the cell

allows some substances to cross more
easily than others
 hydrophobic (nonpolar) vs. hydrophilic (polar)
AP Biology
Arranged as a Phospholipid bilayer
 Serves as a cellular barrier / border
sugar
H 2O
salt
polar
hydrophilic
heads
nonpolar
hydrophobic
tails
impermeable to polar molecules
polar
hydrophilic
heads
waste
AP Biology
lipids
Permeability to polar molecules?
 Membrane becomes semi-permeable via
protein channels

specific channels allow specific material
across cell membrane
inside cell
NH
AP
Biology
3
salt
H 2O
aa
sugar
outside cell
Cell membrane must be more than lipids…
 In 1972, S.J. Singer & G. Nicolson
proposed that membrane proteins are
inserted into the phospholipid bilayer
AP Biology
Membrane is a collage of proteins & other molecules
embedded in the fluid matrix of the lipid bilayer
Glycoprotein
Extracellular fluid
Glycolipid
Phospholipids
Cholesterol
Peripheral
protein
AP Biology
Transmembrane
proteins
Cytoplasm
Filaments of
cytoskeleton
1972, S.J. Singer & G. Nicolson proposed Fluid Mosaic Model
Cell membrane is more than lipids…
 Transmembrane proteins embedded in
phospholipid bilayer
Ideal because of multiple functional
groups within the same protein
 create semi-permeable channels

lipid bilayer
membrane
AP Biology
protein channels
in lipid bilyer membrane
Many Functions of Membrane Proteins
“Channel”
Outside
Plasma
membrane
Inside
Transporter
Enzyme
activity
Cell surface
receptor
Cell adhesion
Attachment to the
cytoskeleton
“Antigen”
AP Biology
Cell surface
identity marker
Membrane Proteins
 Proteins determine membrane’s specific functions

cell membrane & organelle membranes each have
unique collections of proteins
 Classes of membrane proteins:

peripheral proteins
 loosely bound to surface of membrane
 ex: cell surface identity marker (antigens)

integral proteins
 penetrate lipid bilayer, usually across whole membrane
 transmembrane protein
 ex: transport proteins
 channels, permeases (pumps)
AP Biology
Proteins domains anchor molecule
 Within membrane

Polar areas
of protein
nonpolar amino acids
 hydrophobic
 anchors protein
into membrane
 On outer surfaces of
membrane in fluid

polar amino acids
 hydrophilic
 extend into
AP Biology
extracellular fluid &
into cytosol
Nonpolar areas of protein
+
H
H+
Examples
Retinal
chromophore
NH2
aquaporin =
water channel in bacteria
Porin monomer
H 2O
b-pleated sheets
Bacterial
outer
membrane
Nonpolar
(hydrophobic)
a-helices in the
cell membrane
COOH
H++
H
Cytoplasm
proton pump channel
in photosynthetic bacteria
H O
AP Biology 2
function through
conformational change =
protein changes shape
Membrane carbohydrates
 Play a key role in cell-cell recognition

ability of a cell to distinguish one cell
from another
 antigens
important in organ &
tissue development
 basis for rejection of
foreign cells by
immune system

AP Biology
Movement across the
Cell Membrane
AP Biology
Diffusion
 2nd Law of Thermodynamics
governs biological systems

universe tends towards disorder (entropy)
 Diffusion

AP Biology
movement from HIGH  LOW concentration
Simple Diffusion
 Move from HIGH to LOW concentration
“passive transport”
 no energy needed

AP Biology
diffusion
movement of water
osmosis
Facilitated Diffusion
 Diffusion through protein channels


channels move specific molecules across
cell membrane
facilitated = with help
no energy needed
open channel = fast transport
HIGH
LOW
AP Biology
“The Bouncer”
Active Transport
 Cells may need to move molecules against
concentration gradient



conformational shape change transports solute
from one side of membrane to other
protein “pump”
“costs” energy = ATP LOW conformational change
ATP
HIGH
AP Biology
“The Doorman”
Active transport
 Many models & mechanisms
ATP
AP Biology
ATP
antiport
symport
Transport Summary
 Passive Transport

Simple diffusion
 diffusion of nonpolar, hydrophobic molecules
 lipids
 HIGH  LOW concentration gradient

Facilitated transport
 diffusion of polar, hydrophilic molecules
 through a protein channel
 HIGH  LOW concentration gradient
 Active transport

diffusion against concentration gradient
 LOW  HIGH


AP Biology
uses a protein pump
requires ATP
ATP
Transport summary
simple
diffusion
facilitated
diffusion
active
transport
AP Biology
ATP
How about large molecules?
 Moving large molecules into & out of cell
through vesicles & vacuoles
 endocytosis

 phagocytosis = “cellular eating”
 pinocytosis = “cellular drinking”

AP Biology
exocytosis
exocytosis
Endocytosis
phagocytosis
fuse with
lysosome for
digestion
pinocytosis
non-specific
process
receptor-mediated
endocytosis
triggered by
molecular
signal
AP Biology
The Special Case of Water
Movement of water across
the cell membrane
AP Biology
2007-2008
Osmosis is just diffusion of water
 Diffusion of water from
HIGH concentration of water to
LOW concentration of water

AP Biology
across a
semi-permeable
membrane
Concentration of water
 Direction of osmosis is determined by
comparing total solute concentrations

Hypertonic - more solute, less water

Hypotonic - less solute, more water

Isotonic - equal solute, equal water
water
AP Biology
hypotonic
hypertonic
net movement of water
Managing water balance
 Cell survival depends on balancing
water uptake & loss
AP Biology
freshwater
balanced
saltwater
1
Managing water balance
 Hypotonic

a cell in fresh water

high concentration of water around cell
 problem: cell gains water,
swells & can burst
KABOOM!
 example: Paramecium
 ex: water continually enters
Paramecium cell
 solution: contractile vacuole
 pumps water out of cell
ATP
 ATP

plant cells
No problem,
here
 turgid = full
 cell wall protects from bursting
AP Biology
freshwater
Pumping water out
 Contractile vacuole in Paramecium
ATP
AP Biology
2
Managing water balance
 Hypertonic
I’m shrinking,
a cell in salt water I’m shrinking!
 low concentration of water
around cell

 problem: cell loses water &
can die
 example: shellfish
 solution: take up water or
pump out salt
I

plant cells
will
survive!
 plasmolysis = wilt
 can recover
AP Biology
saltwater
3
Managing water balance
 Isotonic
That’s
perfect!

animal cell immersed in
mild salt solution

no difference in concentration of
water between cell & environment
 problem: none
 no net movement of water
flows across membrane equally, in
both directions
I could
 cell in equilibrium
be better…

 volume of cell is stable
 example:
blood cells in blood plasma
 slightly salty IV solution in hospital
AP Biology
balanced
Animation
1991 | 2003
Aquaporins
 Water moves rapidly into & out of cells

evidence that there were water channels
 protein channels allowing flow of water
across cell membrane
AP Biology
Peter Agre
Roderick MacKinnon
John Hopkins
Rockefeller
Do you understand Osmosis…
.05 M
.03 M
Cell (compared to beaker)  hypertonic or hypotonic
Beaker (compared to cell)  hypertonic or hypotonic
Which way does the water flow?  in or out of cell
AP Biology
 Used to describe the movement of water into & out of a cell
 Water potential = pressure potential + solute potential
 Pressure Potential



In plant cell, pressure exerted by rigid cell wall that prevents
further water uptake
Effect of solute concentration
Pure water at atmospheric pressure has solute potential of 0
 Solute Potential



As solute is added, value for solute potential becomes more
negative
Causes water potential to decrease
As solute is added, water potential drops and water tends to
move into solution
AP Biology
•
•
•
•
𝝋s = -iCRT
i = ionization constant
C=Molar concentration
R=Pressure constant
T=Temp in Kelvin
AP Biology
 The molar concentration of a sugar
solution in an open beaker has been
determined to be 0.3M. Calculate the solute
potential at 27°C. Round your answer to the
nearest hundredth
 𝝋s = -iCRT
 𝝋s = -(1) (0.3M) (0.0831) (27 + 273)
 𝝋s = -7.479
AP Biology
 The molar concentration of a sugar
solution in an open beaker has been
determined to be 0.3M. Calculate the solute
potential at 27°C. Round your answer to the
nearest hundredth
 𝝋s = -iCRT
 𝝋s = -(1) (0.3M) (0.0831) (27 + 273)
 𝝋s = -7.479
AP Biology
 The pressure potential of a solution open to

the air is zero. Since you know the solute
potential of the solution, you can now
calculate the water potential.
What is the water potential for this
example? Round your answer to the
nearest hundredth.
 𝝋 = 𝝋𝒑 + 𝝋𝒔
 𝝋 = 𝟎 + (−𝟕. 𝟒𝟕𝟗)
 𝝋 = −𝟕. 𝟒𝟕𝟗
AP Biology
 The pressure potential of a solution open to

the air is zero. Since you know the solute
potential of the solution, you can now
calculate the water potential.
What is the water potential for this
example? Round your answer to the
nearest hundredth.
 𝝋 = 𝝋𝒑 + 𝝋𝒔
 𝝋 = 𝟎 + (−𝟕. 𝟒𝟕𝟗)
 𝝋 = −𝟕. 𝟒𝟕𝟗
AP Biology
 Calculate water potential for each
 Describe what will need to occur for
equilibrium to be reached.
AP Biology
Water Potential
 water potential of pure water in an open
container is zero

no solute and pressure in container is zero
 Adding solute lowers water potential.
 When a solution is enclosed by rigid cell wall,
movement of water into cell will exert
pressure on cell wall.

increases in pressure within cell will raise the
water potential.
AP Biology
Any Questions??
AP Biology