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Chapter 6 Photosynthesis and Respiration Living things need energy to survive. Where do living things get the energy they need? Energy comes in many forms including light, heat, and electricity. Energy can be stored in chemical compounds, too. Cells use Adenosine TriPhosphate to store and release energy ATP ATP is used by all types of cells as their basic energy source. 10 Producers (Autotrophs) Convert the Carbon in CO2 Into Sugars (C6H12O6) using the Energy in SunlightPhotosynthesis ALL CELLS Convert the Energy in the Bonds of Glucose into the Energy of ATP - Respiration • Autotroph • Producer • Heterotroph • Consumer • Chemotroph • Chemosynthesis CO2 C6H12O6 Using Energy of Inorganic Compounds ATP consists of: •adenine •ribose (a 5-carbon sugar) •3 phosphate groups Adenine ATP Ribose 3 Phosphate groups Storing Energy ADP has two phosphate groups instead of three. A cell can store small amounts of energy by adding a phosphate group to ADP. ATP ADP + Adenosine Diphosphate (ADP) + Phosphate Partially charged battery Energy Energy Fully charged battery Adenosine Triphosphate (ATP) Releasing Energy Energy stored in ATP is released by breaking the chemical bond between the second and third phosphates. 2 Phosphate groups P ADP What is the role of ATP in cellular activities? The energy from ATP is needed for many cellular activities, including active transport across cell membranes, protein synthesis and muscle contraction. ATP’s characteristics make it exceptionally useful as the basic energy source of all cells. Using Biochemical Energy Most cells have only a small amount of ATP It is not a good way to store large amounts of energy. Cells can regenerate ATP from ADP as needed by using the energy in foods like glucose. Quiz 6-1 Organisms that make their own food are called a. autotrophs. b. heterotrophs. c. decomposers. d. consumers. Most autotrophs obtain their energy from a. chemicals in the environment. b. sunlight. c. carbon dioxide in the air. d. other producers. How is energy released from ATP? a. A phosphate is added. b. An adenine is added. c. A phosphate is removed. d. A ribose is removed. How is it possible for most cells to function with only a small amount of ATP? a. Cells do not require ATP for energy. b. ATP can be quickly regenerated from ADP and P. c. Cells use very small amounts of energy. d. ATP stores large amounts of energy. Compared to the energy stored in a molecule of glucose, ATP stores a. much more energy. b. much less energy. c. about the same amount of energy. d. more energy sometimes and less at others. Photosynthesis Photosynthesizers use the energy of sunlight to convert water and carbon dioxide into high-energy carbohydrates and oxygen. H2O CO2 Light NADP+ ADP + P Lightdependent reactions Calvin Calvin cycle Cycle Chloroplast O2 Sugars Electron Carriers When electrons in chlorophyll absorb sunlight, the electrons gain a great deal of energy. Cells use electron carriers to transport these high-energy electrons from chlorophyll to other molecules. NADP+ Electron carriers, such as NADP+, transport electrons. NADP+ accepts and holds 2 highenergy electrons along with a hydrogen ion (H+). This converts the NADP+ into NADPH. The Photosynthesis Equation The equation for photosynthesis is: 6CO2 + 6H2O Light carbon dioxide + water C6H12O6 + 6O2 Light sugars + oxygen Light energy H2O Light-Dependent Reactions (thylakoids) ADP + NADP Sugar O2 ATP NADPH Calvin Cycle (stroma) CO2 + H20 Inside a Chloroplast In plants, photosynthesis takes place inside chloroplasts. Plant Chloroplast Plant cells Chloroplasts contain thylakoids—saclike photosynthetic membranes. Single thylakoid Thylakoids are arranged in stacks called grana, Or singular granum. Granum Photosystems, which are the lightcollecting units of the chloroplast are in the thylakoid membrane. Photosystems What is the role of light and chlorophyll in photosynthesis? Light and Pigments How do plants capture the energy of sunlight? In addition to water and carbon dioxide, photosynthesis requires light and chlorophyll. Plants gather the sun's energy with light-absorbing molecules called pigments. The main pigment in plants is chlorophyll. There are two main types of chlorophyll: –chlorophyll a –chlorophyll b Chlorophyll absorbs light well in the blue-violet and red regions of the visible spectrum. Chlorophyll does not absorb light in the green region of the spectrum. Green light is reflected by leaves, which is why plants look green. Light is a form of energy, so any compound that absorbs light also absorbs energy from that light. When chlorophyll absorbs light, much of the energy is transferred directly to electrons in the chlorophyll molecule, raising the energy levels of these electrons. These high-energy electrons are what make photosynthesis work. Photosynthesis Video Quiz 6-2 Most of the added mass of a tree comes from a. soil and carbon dioxide. b. oxygen and carbon dioxide. c. water and carbon dioxide. d. soil and oxygen. Plants use the sugars produced in photosynthesis to make a. oxygen. b. carbon dioxide. c. starches. d. protein. The raw materials required for plants to carry out photosynthesis are a. carbon dioxide and oxygen. b. carbon dioxide and water. c. oxygen and sugars. d. oxygen and water. The principal pigment in plants is a. chloroplast. b. carotene. c. chlorophyll. d. carbohydrate. The colors of light that are absorbed by chlorophylls are a. green and yellow. b. blue, violet, and red. c. green, blue, and violet. d. red and yellow. Light-Dependent Reactions The light-dependent reactions require light. The light-dependent reactions produce oxygen gas and convert ADP and NADP+ into the energy carriers ATP and NADPH. Inside the Thylokoid (Lumen) Outside the Thylokoid (Stroma) Photosynthesis begins when pigments in photosystem II absorb light, increasing their energy level. Photosystem II These high-energy electrons are passed on to the electron transport chain. Photosystem II High-energy electron Electron carriers Enzymes on the thylakoid membrane break water molecules into: Photosystem II 2H2O High-energy electron Electron carriers – hydrogen ions – oxygen atoms – energized electrons Photosystem II + O2 2H2O High-energy electron Electron carriers The energized electrons from water replace the high-energy electrons that chlorophyll lost to the electron transport chain. Photosystem II + 2H2O High-energy electron O2 As plants remove electrons from water, oxygen is left behind and is released into the air. Photosystem II + 2H2O High-energy electron O2 The hydrogen ions left behind when water is broken apart are released inside the thylakoid membrane. Photosystem II + 2H2O High-energy electron O2 Energy from the electrons is used to transport H+ ions from the stroma into the inner thylakoid space. Photosystem II + 2H2O O2 High-energy electrons move through the electron transport chain from photosystem II to photosystem I. Photosystem II + O2 2H2O Photosystem I Pigments in photosystem I use energy from light to re-energize the electrons. + O2 2H2O Photosystem I NADP+ then picks up these high-energy electrons, along with H+ ions, and becomes NADPH. + O2 2H2O 2 NADP+ 2 2 NADPH As electrons are passed from chlorophyll to NADP+, more H+ ions are pumped across the membrane. + O2 2H2O 2 NADP+ 2 2 NADPH Soon, the inside of the membrane fills up with positively charged hydrogen ions, which makes the outside of the membrane negatively charged. + O2 2H2O 2 NADP+ 2 2 NADPH The difference in charges across the membrane provides the energy to make ATP + O2 2H2O 2 NADP+ 2 2 NADPH H+ ions cannot cross the membrane directly. ATP synthase + O2 2H2O 2 NADP+ 2 2 NADPH The cell membrane contains a protein called ATP synthase that allows H+ ions to pass through it ATP synthase + O2 2H2O 2 NADP+ 2 2 NADPH As H+ ions pass through ATP synthase, the protein rotates. ATP synthase + O2 2H2O 2 NADP+ 2 2 NADPH As it rotates, ATP synthase binds ADP and a phosphate group together to produce ATP. ATP synthase + O2 2H2O ADP 2 NADP+ 2 2 NADPH Because of this system, light-dependent electron transport produces not only high-energy electrons but ATP as well. ATP synthase + O2 2H2O ADP 2 NADP+ 2 2 NADPH The Calvin Cycle uses ATP and NADPH from the light-dependent reactions to produce high-energy sugars. The Calvin cycle does not require light Light-Independent reactions. Six carbon dioxide molecules enter the cycle from the atmosphere and combine with six 5-carbon molecules. CO2 Enters the Cycle The result is twelve 3-carbon molecules, which are then converted into higher-energy forms. The energy for this conversion comes from ATP and high-energy electrons from NADPH. Energy Input 12 12 ADP 12 NADPH 12 NADP+ Two of twelve 3-carbon molecules are removed from the cycle. Energy Input 12 12 ADP 12 NADPH 12 NADP+ The molecules are used to produce sugars, lipids, amino acids and other compounds. 12 12 ADP 12 NADPH 12 NADP+ 6-Carbon sugar produced Sugars and other compounds The 10 remaining 3-carbon molecules are converted back into six 5-carbon molecules, which are used to begin the next cycle. 12 12 ADP 6 ADP 12 NADPH 6 12 NADP+ 5-Carbon Molecules Regenerated Sugars and other compounds The two sets of photosynthetic reactions work together. –The Light-Dependent reactions trap sunlight energy in chemical form. Light & H2O NADPH & ATP –The Calvin Cycle uses that chemical energy to produce stable, high-energy sugars from carbon dioxide and water. NADPH, ATP, & CO2 Glucose Quiz 6-3 In plants, photosynthesis takes place inside the a. thylakoids. b. chlorophyll. c. chloroplasts. d. photosystems. Energy to make ATP in the chloroplast comes most directly from a. electrons transferred directly from NADPH. b. transfer of a phosphate from ADP. c. hydrogen ions flowing through an enzyme in the thylakoid membrane. d. electrons moving through the electron transport chain. NADPH is produced in light-dependent reactions and carries energy in the form of a. ATP. b. ADP. c. high-energy electrons. d. low-energy electrons. What is another name for the Calvin cycle? a. light-dependent reactions b. light-independent reactions c. electron transport chain d. photosynthesis Which of the following factors does NOT directly affect photosynthesis? a. light intensity b. water supply c. wind d. temperature Cellular Respiration Chemical Energy and Food One gram of glucose (C6H12O6), when burned, releases 3811 calories of heat energy. Both plant and animal cells carry out the final stages of cellular respiration in the mitochondria. Mitochondrion Animal Cells Outer membrane Inner membrane Matrix Plant Cells Intermembrane space Electrons carried in NADH Pyruvic acid Glucose Electrons carried in NADH and FADH2 Glycolysis Cytoplasm Mitochondrion Cellular respiration releases energy by breaking down glucose and other food molecules in the presence of oxygen. A Cellular Respiration Video Another Cellular Respiration Video The equation for cellular respiration is: 6O2 + C6H12O6 → 6CO2 + 6H2O + Energy oxygen + glucose → carbon dioxide + water + Energy Glycolysis takes place in the cytoplasm. The Krebs cycle and electron transport take place in the mitochondria. Glycolysis Cytoplasm Mitochondrion ATP and NADH Production in Glycolysis 2 ATP 2 ADP 4 ADP 2NAD+ 4 ATP 2 2 Pyruvic acid To the electron transport chain The Advantages of Glycolysis The process of glycolysis is so fast that cells can produce thousands of ATP molecules in a few milliseconds. Glycolysis does not require oxygen. Quiz 6-4 Aerobic respiration produces ATP in … a. eukaryotic animal cells. b. eukaryotic plant cells. c. all eukaryotic cells. d. all prokaryotic and eukaryotic cells. The raw materials required for cellular respiration are a. glucose and oxygen. b. glucose and water. c. carbon dioxide and oxygen. d. carbon dioxide and water. Glycolysis occurs in the a. cytoplasm. b. mitochondria. c. nucleus. d. chloroplasts. The net gain of ATP molecules after glycolysis is a. 2 ATP molecules. b. 3 ATP molecules. c. 3 pyruvic acid molecules. d. 4 pyruvic acid molecules The carbon product of glycolysis is … a. pyruvic acid. b. pyruvate. c. two, three carbon molecules. d. All of the above. Oxygen is required for the final steps of cellular respiration. Because the pathways of cellular respiration require oxygen, they are aerobic. During the Krebs Cycle, pyruvic acid is broken down into carbon dioxide in a series of energy-extracting reactions. The Krebs cycle begins when pyruvic acid produced by glycolysis enters the mitochondrion. One carbon molecule is removed, forming CO2, and electrons are removed, changing NAD+ to NADH. Coenzyme A joins the 2-carbon molecule, forming acetyl-CoA. Acetyl-CoA then adds the 2-carbon acetyl group to a 4carbon compound, forming citric acid. Citric acid Citric acid is broken down into a 5-carbon compound, then into a 4-carbon compound. Two more molecules of CO2 are released and electrons join NAD+ and FADH, forming NADH and FADH2 In addition, one molecule of ATP is generated. The energy tally from 1 molecule of pyruvic acid is – 4 NADH – 1 FADH2 – 1 ATP Electron Transport The electron transport chain uses the high-energy electrons from the Krebs cycle to convert ADP into ATP. High-energy electrons from NADH and FADH2 are passed along the electron transport chain from one carrier protein to the next. At the end of the chain, an enzyme combines these electrons with hydrogen ions and oxygen to form water. As the final electron acceptor of the electron transport chain, oxygen gets rid of the low-energy electrons and hydrogen ions. When 2 high-energy electrons move down the electron transport chain, their energy is used to move hydrogen ions (H+) across the membrane. During electron transport, H+ ions build up in the intermembrane space, so it is positively charged. The other side of the membrane, from which those H+ ions are taken, is now negatively charged. The inner membranes of the mitochondria contain protein spheres called ATP synthases. ATP synthase As H+ ions escape through channels into these proteins, the ATP synthase spins. Channel ATP synthase As it rotates, the enzyme grabs a low-energy ADP, attaching a phosphate, forming high-energy ATP. Channel ATP synthase ADP The Totals Glycolysis produces just 2 ATP molecules per molecule of glucose. The complete breakdown of glucose through cellular respiration, including glycolysis, results in the production of ~36 molecules of ATP. Comparing Photosynthesis and Cellular Respiration The energy flows in photosynthesis and cellular respiration take place in opposite directions. Fermentation When oxygen is not present, glycolysis is followed by a different pathway. The combined process of this pathway and glycolysis is called fermentation. Fermentation releases energy from food molecules by producing ATP in the absence of oxygen. During fermentation, cells convert NADH to NAD+ by passing high-energy electrons back to pyruvic acid. This action converts NADH back into NAD+, and allows glycolysis to continue producing a steady supply of ATP. Fermentation does not require oxygen—it is an anaerobic process. What are the two main types of fermentation? Alcoholic Fermentation Yeasts and a few other microorganisms use alcoholic fermentation, forming ethyl alcohol and carbon dioxide as wastes. The equation for alcoholic fermentation after glycolysis is: pyruvic acid + NADH → alcohol + CO2 + NAD+ Lactic Acid Fermentation In many cells, pyruvic acid that accumulates as a result of glycolysis can be converted to lactic acid. This type of fermentation is called lactic acid fermentation. It regenerates NAD+ so that glycolysis can continue. The equation for lactic acid fermentation after glycolysis is: pyruvic acid + NADH → lactic acid + NAD+ The first part of the equation is glycolysis. The second part shows the conversion of pyruvic acid to lactic acid. On a global level, photosynthesis and cellular respiration are also opposites. – Photosynthesis removes carbon dioxide from the atmosphere and cellular respiration puts it back. – Photosynthesis releases oxygen into the atmosphere and cellular respiration uses that oxygen to release energy from food. Quiz 6-5 The Krebs cycle breaks pyruvic acid down into a. oxygen. b. NADH and FADH2. c. alcohol. d. carbon dioxide. What role does the Krebs cycle play in the cell? a. It transfers energy from molecules formed during glycolysis. b. It breaks down glucose and releases its stored energy. c. It combines carbon dioxide and water into high-energy molecules. d. It breaks down ATP and NADH, releasing stored energy. In respiration, the electron transport chain is located in the a. cell membrane. b. cytoplasm. c. inner mitochondrial membrane. d. outer mitochondrial membrane. To generate energy over long periods, the body must use a. cellular respiration. b. lactic acid fermentation. c. stored ATP. d. glycolysis. Which statement correctly describes photosynthesis and cellular respiration? a. Photosynthesis releases energy, while cellular respiration stores energy. b. Photosynthesis and cellular respiration use the same raw materials. c. Cellular respiration and photosynthesis produce the same products. d. Cellular respiration releases energy, while photosynthesis stores energy. Homework!!! Chapter 6 Standardized Test Prep