Figure S1
... Figure S8. Effect of Smyd2 overexpression on the levels of p21 promoter-associated p53. a, Western blot analysis of Smyd2 in lysates from U2OS/Lenti6/TR cells inducibly expressing vector (Ctr.) (lanes 1 and 2) or FLAG-Smyd2 (f-Smyd2) (lanes 3 and 4) treated without (lanes 1 and 3) or with (lanes 2 a ...
... Figure S8. Effect of Smyd2 overexpression on the levels of p21 promoter-associated p53. a, Western blot analysis of Smyd2 in lysates from U2OS/Lenti6/TR cells inducibly expressing vector (Ctr.) (lanes 1 and 2) or FLAG-Smyd2 (f-Smyd2) (lanes 3 and 4) treated without (lanes 1 and 3) or with (lanes 2 a ...
Ten novel interaction partners for the histone H2A protein
... Among all the clones, the ones with the strongest Nub fusion protein interaction with Hta1Cub-RUra3p were S7 and S13, with an average relative score of 2.8. The weakest interaction occurred in S12, with a score of 1.0. Nub-Htb1 had the highest average score of 3.3. All the other clones had averages ...
... Among all the clones, the ones with the strongest Nub fusion protein interaction with Hta1Cub-RUra3p were S7 and S13, with an average relative score of 2.8. The weakest interaction occurred in S12, with a score of 1.0. Nub-Htb1 had the highest average score of 3.3. All the other clones had averages ...
Biochemistry Guided Notes
... 2. _________breaks down ______________(liver) into glucose 3. _______________(speeds up chemical reactions) What is the name of the process that makes proteins? _____________________ What is the name of the process that breaks down proteins? ________________ What is the name molecule formed from two ...
... 2. _________breaks down ______________(liver) into glucose 3. _______________(speeds up chemical reactions) What is the name of the process that makes proteins? _____________________ What is the name of the process that breaks down proteins? ________________ What is the name molecule formed from two ...
Inquiry into Life Twelfth Edition
... • Two families of 5S rRNA genes studied are oocyte and somatic genes – Oocyte genes are expressed only in oocytes – Somatic genes are expressed both in oocytes and somatic cells – Somatic genes form more stable complexes with transcription factors ...
... • Two families of 5S rRNA genes studied are oocyte and somatic genes – Oocyte genes are expressed only in oocytes – Somatic genes are expressed both in oocytes and somatic cells – Somatic genes form more stable complexes with transcription factors ...
Chapter 3 - Huntsville High School
... macromolecules because they can be very large • There are four types of macromolecules: 1. Proteins 2. Nucleic acids 3. Carbohydrates 4. Lipids • Large macromolecules are actually assembled from many similar small components, called monomers – the assembled chain of monomers is known as a polymer ...
... macromolecules because they can be very large • There are four types of macromolecules: 1. Proteins 2. Nucleic acids 3. Carbohydrates 4. Lipids • Large macromolecules are actually assembled from many similar small components, called monomers – the assembled chain of monomers is known as a polymer ...
Histone Deacetylase 4 Antibody
... Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past se ...
... Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past se ...
Cancer Lab p53 – Teacher Background
... The MDM2 gene is the target gene of the transcription factor p53 protein. The encoded MDM2 protein is a nuclear phosphoprotein that binds and inhibits transactivation by the p53 protein, as part of an auto-regulatory negative feedback loop. If MDM2 gene is overexpressed, it can result in the excessi ...
... The MDM2 gene is the target gene of the transcription factor p53 protein. The encoded MDM2 protein is a nuclear phosphoprotein that binds and inhibits transactivation by the p53 protein, as part of an auto-regulatory negative feedback loop. If MDM2 gene is overexpressed, it can result in the excessi ...
Analysis of hepatocyte nuclear factor
... development. Toward this goal, functional dissection of numerous hepatocyte-specific promoter and enhancer regions has revealed that they are structurally complex, consisting of multiple DNA binding sites recognized by distinct families of liverenriched transcription factors (1). The combinatorial a ...
... development. Toward this goal, functional dissection of numerous hepatocyte-specific promoter and enhancer regions has revealed that they are structurally complex, consisting of multiple DNA binding sites recognized by distinct families of liverenriched transcription factors (1). The combinatorial a ...
Protein synthesis
... The ultimate cellular location of proteins is often determined by specific, relatively short amino acid sequence within the proteins themselves. These sequences can be responsible for proteins being secreted, imported into the nucleus or targeted to other organelles. ...
... The ultimate cellular location of proteins is often determined by specific, relatively short amino acid sequence within the proteins themselves. These sequences can be responsible for proteins being secreted, imported into the nucleus or targeted to other organelles. ...
Silica supported zinc chloride catalyzed acetylation of amines
... generally give higher yield of products and can be recycled for a number of times. The heterogeneous catalysts have been proven to offer similar levels of activity to their homogeneous counterparts. The majority of these novel heterogeneous catalysts are based primarily on silica, since silica displ ...
... generally give higher yield of products and can be recycled for a number of times. The heterogeneous catalysts have been proven to offer similar levels of activity to their homogeneous counterparts. The majority of these novel heterogeneous catalysts are based primarily on silica, since silica displ ...
trypsin inhibitor and castor-bean (Ricinus communis) storage protein
... minimum mutation distance value for this 73alignment (residues 1-74, with two gaps) is 76, giving a 'significance level' (matching probability) of 0.1886 x 10-6, a value that defines an alignment as 'highly significant' (Moore & Goodman, 1977; Vogel, 1978). This result strongly suggests a common anc ...
... minimum mutation distance value for this 73alignment (residues 1-74, with two gaps) is 76, giving a 'significance level' (matching probability) of 0.1886 x 10-6, a value that defines an alignment as 'highly significant' (Moore & Goodman, 1977; Vogel, 1978). This result strongly suggests a common anc ...
How oncoproteins regulate gene expression
... characterisations had shown, a down regulation of MiR-34 is enough to cause a significant decrease in p53 mediated apoptosis and cellular senescence (He et al., 2009). The two studies which I have chosen to highlight are prime examples of a new approach to studying oncoprotein regulation; although w ...
... characterisations had shown, a down regulation of MiR-34 is enough to cause a significant decrease in p53 mediated apoptosis and cellular senescence (He et al., 2009). The two studies which I have chosen to highlight are prime examples of a new approach to studying oncoprotein regulation; although w ...
Метод поиска SDP
... • A method for identification of amino acid residues that account for differences in protein functional specificity – Does not rely on the protein 3D structure – Automatically determines the number of significant positions – Considers substitutions according to the chemical properties of substituted ...
... • A method for identification of amino acid residues that account for differences in protein functional specificity – Does not rely on the protein 3D structure – Automatically determines the number of significant positions – Considers substitutions according to the chemical properties of substituted ...
Protein expression during exponential growth in 0.7 M NaCl medium
... 35S-methionine, followed by protein separation and quantification by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) combined with computerised image analysis. The electrophoretic separation resolved about 650 proteins of which 13 displayed significant and at least 2-fold changes in rat ...
... 35S-methionine, followed by protein separation and quantification by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) combined with computerised image analysis. The electrophoretic separation resolved about 650 proteins of which 13 displayed significant and at least 2-fold changes in rat ...
L3 Physicochemical properties of proteins - e
... protein do not need to interact with each other. ...
... protein do not need to interact with each other. ...
Robustness of the model
... exact test: 0.07, see Table S6). However, when we considered bottlenecks that are not hubs, coiled-coil proteins were most over-represented (25% vs. 13%, pvalue 0.11). To control for hubs regardless of their functional class, it is also possible the assign weights to the edges based on the normalize ...
... exact test: 0.07, see Table S6). However, when we considered bottlenecks that are not hubs, coiled-coil proteins were most over-represented (25% vs. 13%, pvalue 0.11). To control for hubs regardless of their functional class, it is also possible the assign weights to the edges based on the normalize ...
Morphologically distinct phenotypes of spermatozoa in infertile men
... RESULTS: A total of 1202 proteins were identified in the F1 fraction while 1140, 1025 and 890 proteins were recovered from the three other fractions, F2, F3 and F4 respectively. With respect to the differentially expressed proteins, F1 exhibited the highest number (522), followed by F2 (362) and low ...
... RESULTS: A total of 1202 proteins were identified in the F1 fraction while 1140, 1025 and 890 proteins were recovered from the three other fractions, F2, F3 and F4 respectively. With respect to the differentially expressed proteins, F1 exhibited the highest number (522), followed by F2 (362) and low ...
Introduction to Biochemistry The Sun provides Energy for Life
... not to ES. (Substrate cannot bind. Km increases.) • Noncompetitive inhibition: Inhibitor (I) binds either to E and/or to ES. (V max decreases and Km can increase, decrease or remain constant.) ...
... not to ES. (Substrate cannot bind. Km increases.) • Noncompetitive inhibition: Inhibitor (I) binds either to E and/or to ES. (V max decreases and Km can increase, decrease or remain constant.) ...
IOSR Journal of Applied Chemistry (IOSR-JAC)
... transformation [1]. Diverse reagents and reaction conditions have been developed for this purpose. Conversion of aromatic amines to corresponding acetamides is also well documented [2]. Reduction of nitro group and acetylation of the resulting amine are often carried out in succession to attenuate t ...
... transformation [1]. Diverse reagents and reaction conditions have been developed for this purpose. Conversion of aromatic amines to corresponding acetamides is also well documented [2]. Reduction of nitro group and acetylation of the resulting amine are often carried out in succession to attenuate t ...
The Exocytic/Lysosomal Transport Pathway
... • COPI vesicles retrieve ER proteins and return them • KDEL signal on lumenal proteins • KKxx-COOH on TM proteins (dilysine motif) ...
... • COPI vesicles retrieve ER proteins and return them • KDEL signal on lumenal proteins • KKxx-COOH on TM proteins (dilysine motif) ...
Tiffany Hough Presentation
... Identify NPHP complexes and proteins with which they interact during proper vision: Molecular pathway examined in a tissue specific manner Identify other proteins that interact specifically with CEP290 to help explain rescue by Nterminus ...
... Identify NPHP complexes and proteins with which they interact during proper vision: Molecular pathway examined in a tissue specific manner Identify other proteins that interact specifically with CEP290 to help explain rescue by Nterminus ...
Seminar 7 – Theoretical part is in the exercise 4. Seminar 7
... 4. Name two uncommon amino acids that occur in proteins. By what route do they get into proteins? ...
... 4. Name two uncommon amino acids that occur in proteins. By what route do they get into proteins? ...
Catalysts 1
... also been successfully used. In 2004, Phukan used iodine as a catalyst for the acetylation of alcohols under solvent-free conditions [34]. There have also been reports on the acylation of alcohols using acetic anhydride, catalyzed by silica gel supported Ce(SO4)2, Ti(SO4)2, Fe2(SO4)3, and NaHSO4 [35 ...
... also been successfully used. In 2004, Phukan used iodine as a catalyst for the acetylation of alcohols under solvent-free conditions [34]. There have also been reports on the acylation of alcohols using acetic anhydride, catalyzed by silica gel supported Ce(SO4)2, Ti(SO4)2, Fe2(SO4)3, and NaHSO4 [35 ...
Supplementary Information
... MS/MS spectrum used for identification of Cyp18, control CoIP experiments, a further experiment for identification of the interaction of purified recombinant p53 and Cyp18 proteins by MS, p53 basic activation after temperature shift (37°C 32°C), p53 activation and p53-target gene expression after ...
... MS/MS spectrum used for identification of Cyp18, control CoIP experiments, a further experiment for identification of the interaction of purified recombinant p53 and Cyp18 proteins by MS, p53 basic activation after temperature shift (37°C 32°C), p53 activation and p53-target gene expression after ...
Acetylation
Acetylation (or in IUPAC nomenclature ethanoylation) describes a reaction that introduces an acetyl functional group into a chemical compound. (Deacetylation is the removal of the acetyl group.)Acetylation refers to the process of introducing an acetyl group (resulting in an acetoxy group) into a compound, namely the substitution of an acetyl group for an active hydrogen atom. A reaction involving the replacement of the hydrogen atom of a hydroxyl group with an acetyl group (CH3 CO) yields a specific ester, the acetate. Acetic anhydride is commonly used as an acetylating agent reacting with free hydroxyl groups. For example, it is used in the synthesis of aspirin and heroin.