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in Power-Point Format
in Power-Point Format

... • Promoters recognized by pol II (class II promoters) are similar to prokaryotic promoters: • Considered to have two parts: – Core promoter of 4 elements: TATAAA, TBP, BRE (IIB), ...
DNA/RNA Set - Edgerton Center
DNA/RNA Set - Edgerton Center

... central dogma of molecular biology.................. P17 messenger RNA................................................. P17 transfer RNA .................................................... P17 translation......................................................... P17 amino acid....................... ...
Nucleic Acids and Proteins
Nucleic Acids and Proteins

... replaces it with DNA. A nick is left where two nucleotides are still unconnected. 8. DNA ligase seals up the nick by making another sugar-phosphate bond (joins up the bits of DNA to strand) Some important points to note are as follows:  During replication each new unit added to the growing DNA poly ...
49 fv protein synth.p65
49 fv protein synth.p65

... which bud off from the rough endoplasmic reticulum, migrate through the cytoplasm and fuse with the cisternae (cavities) of the Golgi body. Here (and also in the rough endoplasmic reticulum and its vesicles) the polypeptides couple by hydrogen bonding and sulphur bonding, between amino acid side cha ...
DNA/RNA Set - MIT Edgerton Center
DNA/RNA Set - MIT Edgerton Center

... central dogma of molecular biology.................. P17 messenger RNA................................................. P17 transfer RNA .................................................... P17 translation......................................................... P17 amino acid....................... ...
Polymerase Chain Reaction
Polymerase Chain Reaction

... Prokaryote: Cell or organism lacking a membrane-bound, structurally discrete nucleus and other subcellular compartments. Bacteria are prokaryotes. Compare eukaryote. See chromosomes. Promoter: A site on DNA to which RNA polymerase will bind and initiate transcription. Protein: A large molecule compo ...
chapter 13 section 2 notes
chapter 13 section 2 notes

... peptide bond between the first and second amino acids— methionine and phenylalanine. At the same time, the bond holding the first tRNA molecule to its amino acid is broken. ...
Glossary Excerpted with modification from the Glossary in Genes V
Glossary Excerpted with modification from the Glossary in Genes V

... tested in pairwise combinations in trans; defines a genetic unit (the cistron) that might better be called a noncomplementation group. Conditional lethal mutations kill a cell or virus under certain (nonpermissive) conditions, but allow it to survive under other (permissive) conditions. Conjugation ...
A Survey of Intron Research in Genetics
A Survey of Intron Research in Genetics

... in gure 3. Initiation sites contain zero or more regulator regions and a promoter region. Regulator regions inhibit or allow the expression of a gene. The promoter regions are recognized by an enzyme called RNA polymerase as starting points for transcription. Transcription of the gene continues unt ...
MICROBIAL GENETICS
MICROBIAL GENETICS

... Protein Synthesis How is the information in DNA used to make the proteins that control cell activities? In the process of transcription, genetic information in DNA is copied, or transcribed, into a complementary base sequence of RNA. The cell then uses the information encoded in this RNA to synthes ...
4a - digbio
4a - digbio

...  Amount of material available (mRNA, slides, dyes). ...
Isr J Chem (2010) - Weizmann Institute of Science
Isr J Chem (2010) - Weizmann Institute of Science

... dynamic tunnel and in eubacteria they emerge into a shelter formed by ribosome-bound trigger-factor which acts as a “chaperone” to prevent aggregation and misfolding. The peptidyl transferase center (PTC) is the primary catalytic core of the ribosome. It is responsible for promoting the chemical cat ...
The Genetics of Viruses and Prokaryotes The Genetics of Viruses
The Genetics of Viruses and Prokaryotes The Genetics of Viruses

... • The first to describe viruses was Beijerinck (1898), a Dutch microbial ecologist who showed that they were not killed by alcohol, did not grow on any media, and only reproduced inside a host. ...
ACADEMIC BIOLOGY MIDTERM REVIEW GUIDE
ACADEMIC BIOLOGY MIDTERM REVIEW GUIDE

... 10. What are sex-linked traits? 11. Who shows more sex linked traits? 12. What is a carrier? Can a male be a carrier? 13. Be able to solve sex-linked punnett squares and give genotypic and phenotypic ratio of offspring. 14. Name two sex linked traits 15. What can be learned by looking at a karyotype ...
Chapter Four - people.iup.edu
Chapter Four - people.iup.edu

... • Transcription (DNA to RNA) is carried out by RNA polymerase (Rpol) using DNA as template with RNA chain growth in 5’ to 3’ direction. • Transciption begins at a DNA sequence called a promoter. Also requires sigma factor or start factor. • Transcription stops at specific sites called ...
Polyamines and other charged amines bind to RNA by hydrogen
Polyamines and other charged amines bind to RNA by hydrogen

... vivo» and they may be important in regulating the biological function of some nucleic acids1"1*. The studies of Gosule and Schellman 5 and of Flink and Pettijohn6 indicate that spermine, NH 2 (CH 2 ) 3 NH(CH 2 ) 1) NH(CH 2 ) NH 2 , may play an important role in packaging DNA in bacterial cells and i ...
Pseudogene function: regulation of gene expression
Pseudogene function: regulation of gene expression

... A second functional (pseudo)gene, occurring in tandem with the first one (see Figure 2) is now known to exist: ‘Note that in antiNOS-1 the antisense region is located at the 5’ end of the molecule, whereas in antiNOS-2 it is located at the 3’ end. Another important difference is that although antiNOS ...
Supplementary figure legends
Supplementary figure legends

... fluorescence. The simulated period of time was longer than 60 days. The results obtained with long half-lived mRNA and proteins are shown on the left side (panels A, C, E and G) and those with short half-lived proteins and mRNA on the right side (panels B, D, F and H). A and B show the state of the ...
Expression Profiling of Fixed and Unfixed Tissue - Sigma
Expression Profiling of Fixed and Unfixed Tissue - Sigma

... OmniPlex library synthesis followed by library amplification. To make the library, isolated RNA from fixed or unfixed tissue is first annealed to a nonself complementary primer comprised of a quasi random 3' end and a universal 5' end. The primer is extended by polymerase, displacing a single strand ...
Translation
Translation

... •tRNA looks for the codon that pairs with its anticodon ...
DNA and RNA - davis.k12.ut.us
DNA and RNA - davis.k12.ut.us

... you now have exposed nitrogen bases. Attach six mRNA nucleotides to your original DNA strand. Remember T (thymine) is replaced by U (uracil) when making RNA. Because mRNA is single stranded, it is only formed on one half of your DNA. Remove your mRNA strand from the DNA strand and put your DNA stran ...
Chapter 21
Chapter 21

... – May allow scientists to find cause-and-effect relationships between genetic profiles and disorders caused by multifactorial genes ...
современные проблемы молекулярной биологии
современные проблемы молекулярной биологии

... A Promoter, CAP, leader, Coding region, stop codon, trailer, poly(A) tail B CAP, Promoter, leader, Coding region, stop codon, trailer, poly(A) tail C Promoter, CAP, leader, Coding region, stop codon, poly(A) tail, trailer, D Promoter, leader, CAP, Coding region, stop codon, trailer, poly(A) tail E P ...
MicroarraysExp
MicroarraysExp

... The supercoiling of the DNA may also control how the coding regions are used. As well, there is a lot of DNA which appears to be "junk" - i.e. to date no function is known. But we keep making new discoveries e.g. some of the "junk" codes for small RNA pieces that are functional. ...
Chapter 15: Translation of mRNA
Chapter 15: Translation of mRNA

... After introducing the genetic code, tRNAs and the ribosome, we are prepared to discuss the process of translation. Note that translation involves three stages – initiation, elongation, and termination. Although this is the same terminology as was used in transcription, the processes are very differe ...
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Non-coding RNA



A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA (npcRNA), non-messenger RNA (nmRNA) and functional RNA (fRNA). The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene.Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNAs and the long ncRNAs that include examples such as Xist and HOTAIR (see here for a more complete list of ncRNAs). The number of ncRNAs encoded within the human genome is unknown; however, recent transcriptomic and bioinformatic studies suggest the existence of thousands of ncRNAs., but see Since many of the newly identified ncRNAs have not been validated for their function, it is possible that many are non-functional. It is also likely that many ncRNAs are non functional (sometimes referred to as Junk RNA), and are the product of spurious transcription.
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