University of Groningen Methionine
... Impact Statement This study demonstrates methionine-mediated gene regulation in Streptococcus pneumoniae and identifies methionine transport and biosynthesis genes. S. pneumoniae is a human nasopharyngeal pathogen that is responsible for millions of deaths each year. Methionine is one of the importa ...
... Impact Statement This study demonstrates methionine-mediated gene regulation in Streptococcus pneumoniae and identifies methionine transport and biosynthesis genes. S. pneumoniae is a human nasopharyngeal pathogen that is responsible for millions of deaths each year. Methionine is one of the importa ...
The Chicken (Gallus gallus) Z Chromosome Contains at Least Three
... mechanisms for such segmental steps of sex chromosome divergence are inversions on Y (W) and recombination restriction without inversions. Neither of these alternatives can currently be tested, as the poor assembly of the repeat-rich chicken W chromosome precludes gene-order analysis, and no antagon ...
... mechanisms for such segmental steps of sex chromosome divergence are inversions on Y (W) and recombination restriction without inversions. Neither of these alternatives can currently be tested, as the poor assembly of the repeat-rich chicken W chromosome precludes gene-order analysis, and no antagon ...
Slide set - Mediterranean Group for the Study of Diabetes
... specific genetic polymorphisms (SNPs and VNTRs) related to glucose and lipid metabolism as well as inflammation in a high T2DM risk Central Mediterranean population. • To explore the applicability of an allele scoring approach in determining the risk of pGDM. ...
... specific genetic polymorphisms (SNPs and VNTRs) related to glucose and lipid metabolism as well as inflammation in a high T2DM risk Central Mediterranean population. • To explore the applicability of an allele scoring approach in determining the risk of pGDM. ...
מצגת של PowerPoint - Tel Aviv University
... the organism to produce double the amount of the derived protein. 3. The two copies can diverge and each one will specialize in a different function. ...
... the organism to produce double the amount of the derived protein. 3. The two copies can diverge and each one will specialize in a different function. ...
Targeting Cancer Metabolism
... by the ratio of oxidative phosphorylation and glycolysis that reflects metabolic activity and may correlate with response to chemotherapy (31). Some human carcinomas overexpress mitochondrial ATPase inhibitory factor 1(IF1), which blocks the activity of mitochondrial Hþ-ATP synthase and facilitates ...
... by the ratio of oxidative phosphorylation and glycolysis that reflects metabolic activity and may correlate with response to chemotherapy (31). Some human carcinomas overexpress mitochondrial ATPase inhibitory factor 1(IF1), which blocks the activity of mitochondrial Hþ-ATP synthase and facilitates ...
Yvonne Gicheru Presentation
... - Opdc binds to some target sequences and this less strongly than the WT • Transactivation of luciferase reporter gene under Pax2 target sequence using CMV constructs transfected into NIH3 fibroblast cells ...
... - Opdc binds to some target sequences and this less strongly than the WT • Transactivation of luciferase reporter gene under Pax2 target sequence using CMV constructs transfected into NIH3 fibroblast cells ...
EOBII Controls Flower Opening by Functioning as
... repeat) plants. These transgenic plants were generated in the MD genetic background by leaf-disc transformation (Jorgensen et al., 1996; Underwood et al., 2005; Dexter et al., 2007; Colquhoun et al., 2010a). A striking phenotype was visually observable when the ir-PhEOBII plants were grown to a matu ...
... repeat) plants. These transgenic plants were generated in the MD genetic background by leaf-disc transformation (Jorgensen et al., 1996; Underwood et al., 2005; Dexter et al., 2007; Colquhoun et al., 2010a). A striking phenotype was visually observable when the ir-PhEOBII plants were grown to a matu ...
a2 - Molecular and Cell Biology
... or of the genes in item 2, but active. Whichever genes are contained in copy #3 determines the mating type. ...
... or of the genes in item 2, but active. Whichever genes are contained in copy #3 determines the mating type. ...
A novel procedure for genotyping of single nucleotide polymorphisms in trisomy with genomic DNA and the invader assay.
... can accurately determine genotypes in trisomic DNA samples are expensive, require specialized equipment and complicated analyses. Here we report proof-of-concept results for an InvaderÕ assay-based genotyping procedure that can determine SNP genotypes in trisomic genomic DNA samples in a simple and ...
... can accurately determine genotypes in trisomic DNA samples are expensive, require specialized equipment and complicated analyses. Here we report proof-of-concept results for an InvaderÕ assay-based genotyping procedure that can determine SNP genotypes in trisomic genomic DNA samples in a simple and ...
The Mouse Immunoglobulin Heavy Chain V
... using the RNeasy kit (Qiagen). DNA was removed using the RQ1 DNase I kit (Promega). The RNA was repurified using the RNeasy kit and RNA cleanup protocol (Qiagen). 1 g of RNA was reverse transcribed using 100 ng of random hexamers (Amersham Biosciences) and Superscript III (Invitrogen) at 50 °C for ...
... using the RNeasy kit (Qiagen). DNA was removed using the RQ1 DNase I kit (Promega). The RNA was repurified using the RNeasy kit and RNA cleanup protocol (Qiagen). 1 g of RNA was reverse transcribed using 100 ng of random hexamers (Amersham Biosciences) and Superscript III (Invitrogen) at 50 °C for ...
Mutation screening of phenylketonuria in the Far East of
... region (Charikova et al. 1993) was comparable to that found in the present investigation. They found that the R158Q and R261Q mutations were at low frequencies; however, the IVS12nt-1 mutation was more prevalent. This may be the effect of gene flow from the Northern European population into the Mosc ...
... region (Charikova et al. 1993) was comparable to that found in the present investigation. They found that the R158Q and R261Q mutations were at low frequencies; however, the IVS12nt-1 mutation was more prevalent. This may be the effect of gene flow from the Northern European population into the Mosc ...
human tRNA(m22G26)dimethyltransferase: functional expression
... mediates the formation of one specific modification, but does so at more than one site (16–19). The yeast enzyme tRNA(m22G)dimethyltransferase, encoded by a single nuclear TRM1 gene (20,21) modifies G26 to m22G in both nuclear encoded and mitochondrial yeast tRNAs (21,22). TRM1 homologs from several ...
... mediates the formation of one specific modification, but does so at more than one site (16–19). The yeast enzyme tRNA(m22G)dimethyltransferase, encoded by a single nuclear TRM1 gene (20,21) modifies G26 to m22G in both nuclear encoded and mitochondrial yeast tRNAs (21,22). TRM1 homologs from several ...
Tiie Need for Bioinformatics in Evo-Devo
... Modularity has been argued to be the most critical aspect of order in living organisms and their ontogenies, and the attribute that most strongly facilitates evolution (Raff 1996). Modularity brings together development and evolution in several respects (Schlosser and Wagner 2004). First, we know th ...
... Modularity has been argued to be the most critical aspect of order in living organisms and their ontogenies, and the attribute that most strongly facilitates evolution (Raff 1996). Modularity brings together development and evolution in several respects (Schlosser and Wagner 2004). First, we know th ...
Biogeochemical gradients and genomics of denitrifying microbial
... the results were complicated by amplification in the negative control sample, which contained RNA that had undergone the cDNA synthesis reaction without reverse transcriptase. The source of this amplification could not be determined; a PCR reaction with the nirK primer pair and bacterial RNA samples ...
... the results were complicated by amplification in the negative control sample, which contained RNA that had undergone the cDNA synthesis reaction without reverse transcriptase. The source of this amplification could not be determined; a PCR reaction with the nirK primer pair and bacterial RNA samples ...
Genomic imprinting and human disease
... Mammalian genomic imprinting is an epigenetic marking phenomenon leading to mono-allelic expression of a subset of genes [1]. Mono-allelic expression depends entirely on the parental origin of the gene. Thus some imprinted genes are expressed only from the maternally inherited allele, whereas others ...
... Mammalian genomic imprinting is an epigenetic marking phenomenon leading to mono-allelic expression of a subset of genes [1]. Mono-allelic expression depends entirely on the parental origin of the gene. Thus some imprinted genes are expressed only from the maternally inherited allele, whereas others ...
Induction of XIST expression from the human active
... the chromosome. To address this, the transcriptional status of seven genes subject to X chromosome inactivation (PGK1, CCG1, TIMP1, T-plastin, AR, IDSX and G6PD) and one gene which escapes X chromosome inactivation (RPS4X) was analysed by RT–PCR in RNA isolated from the various clones (Fig. 4). The ...
... the chromosome. To address this, the transcriptional status of seven genes subject to X chromosome inactivation (PGK1, CCG1, TIMP1, T-plastin, AR, IDSX and G6PD) and one gene which escapes X chromosome inactivation (RPS4X) was analysed by RT–PCR in RNA isolated from the various clones (Fig. 4). The ...
video slide - Mrs. H-D
... Figure 18.4a, b (a) Tobacco mosaic virus (b) Adenoviruses Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
... Figure 18.4a, b (a) Tobacco mosaic virus (b) Adenoviruses Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
Lecture9_10_extra2 - Welcome to people.pharmacy.purdue.edu!
... HLA-DQ, HLA-DP, HLA-DR Each MHC II locus encodes a gene for the chain and a gene for the chain: e.g. HLA-DQA, HLA-DQB => MHC II isoforms HLA-DPA, HLA-DPB => MHC II isoforms HLA-DRA, HLA-DRB => MHC II isoforms ...
... HLA-DQ, HLA-DP, HLA-DR Each MHC II locus encodes a gene for the chain and a gene for the chain: e.g. HLA-DQA, HLA-DQB => MHC II isoforms HLA-DPA, HLA-DPB => MHC II isoforms HLA-DRA, HLA-DRB => MHC II isoforms ...
Optimizing Data Acquisition for Automated de novo Sequencing
... Objective), flow rate 300 nL/min. A gradient of 5 - 40% acetonitrile in 40 minutes was used. The tandem mass spectrometric analysis was performed on the LTQ Orbitrap (Thermo Fisher Scientific). The so called ‘Top3’ acquisition routine was used, comprising a full MS scan followed by 3 Data Dependent™ ...
... Objective), flow rate 300 nL/min. A gradient of 5 - 40% acetonitrile in 40 minutes was used. The tandem mass spectrometric analysis was performed on the LTQ Orbitrap (Thermo Fisher Scientific). The so called ‘Top3’ acquisition routine was used, comprising a full MS scan followed by 3 Data Dependent™ ...
General background text Pharmacogenetics
... The process of genotyping is used to determine the genotype. It indicates which alleles of the gene for TPMT are present in the tested individual. Each allele has a name that consists of a star (*) and a number, an example of a possible TPMT genotype is TPMT*1/*3A. Many variations exist for TPMT, mo ...
... The process of genotyping is used to determine the genotype. It indicates which alleles of the gene for TPMT are present in the tested individual. Each allele has a name that consists of a star (*) and a number, an example of a possible TPMT genotype is TPMT*1/*3A. Many variations exist for TPMT, mo ...
Genetics lectures 1
... (The offspring from a cross of two true breeding lines is known as the F1 or first filial generation). The F1 flies appear like wild type therefore shi– is recessive (not expressed in heterozygote) Say we have isolated a new paralyzed mutant that we call par par. We start with a true breeding par– s ...
... (The offspring from a cross of two true breeding lines is known as the F1 or first filial generation). The F1 flies appear like wild type therefore shi– is recessive (not expressed in heterozygote) Say we have isolated a new paralyzed mutant that we call par par. We start with a true breeding par– s ...
Forward to the special issue on Hox/Tale transcription factors in
... findings are reviewed, although continuing work in the field will determine whether this represents another general mechanism. Although there are many features of Hox proteins that make them particularly difficult to study, many in the field would agree that there are two main impediments toward mak ...
... findings are reviewed, although continuing work in the field will determine whether this represents another general mechanism. Although there are many features of Hox proteins that make them particularly difficult to study, many in the field would agree that there are two main impediments toward mak ...
PureYield™ RNA Midiprep System Technical Manual
... Note: This system has not been developed to isolate RNA from chemically preserved samples (e.g., formalin-fixed or paraffin-embedded tissues). The PureYield™ RNA Midiprep System provides many unique features to purify total RNA without using DNase treatment, phenol:chloroform extractions, protease d ...
... Note: This system has not been developed to isolate RNA from chemically preserved samples (e.g., formalin-fixed or paraffin-embedded tissues). The PureYield™ RNA Midiprep System provides many unique features to purify total RNA without using DNase treatment, phenol:chloroform extractions, protease d ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.