Super-resolution Microscopy
... samples with a resolution similar to that of an electron microscope, yet it retains the advantages of an optical fluorescence microscope. This means it is possible to uniquely visualize desired molecular species in a cellular environment, even in three dimensions and now in live cells – all at a sca ...
... samples with a resolution similar to that of an electron microscope, yet it retains the advantages of an optical fluorescence microscope. This means it is possible to uniquely visualize desired molecular species in a cellular environment, even in three dimensions and now in live cells – all at a sca ...
Image Quality Criteria - University of Arizona
... the system is built, those metrics also need to be tested to verify that the imaging system will be able to meet its objectives and that the engineering practices used to design the system are valid and can be used again with confidence. Selection of metrics that can be accurately modeled and tested ...
... the system is built, those metrics also need to be tested to verify that the imaging system will be able to meet its objectives and that the engineering practices used to design the system are valid and can be used again with confidence. Selection of metrics that can be accurately modeled and tested ...
Imaging the Division Process in Living Tissue Culture Cells
... portion of the spectrum. This limitation will likely be overcome in the near future, as cameras are developed that are more homogeneously sensitive across the visible spectrum, but for now it remains an important practical consideration. In addition to choosing the correct wavelength, care should b ...
... portion of the spectrum. This limitation will likely be overcome in the near future, as cameras are developed that are more homogeneously sensitive across the visible spectrum, but for now it remains an important practical consideration. In addition to choosing the correct wavelength, care should b ...
Three-dimensional imaging by optical sectioning in the aberration
... to fully understand the three-dimensional structure of materials that determines their chemical, physical and electronic properties. At present, the most common technique for three-dimensional imaging is electron tomography (Midgley & Weyland 2003), and while the volume resolution of this technique ...
... to fully understand the three-dimensional structure of materials that determines their chemical, physical and electronic properties. At present, the most common technique for three-dimensional imaging is electron tomography (Midgley & Weyland 2003), and while the volume resolution of this technique ...
3D differential interference contrast microscopy using synthetic
... x-axis Opposite is the case for the structure indicated by a blue arrow. This shows the strength of aperture synthesis in post-processing. In addition to the contrast enhancement, we also implement depth-selective visualization by angular spectrum method (Chapter 3 in Goodman).10 Conventional hologr ...
... x-axis Opposite is the case for the structure indicated by a blue arrow. This shows the strength of aperture synthesis in post-processing. In addition to the contrast enhancement, we also implement depth-selective visualization by angular spectrum method (Chapter 3 in Goodman).10 Conventional hologr ...
Document
... displaying it in a visual form [8]. Examples of such physical properties include absorption (e.g. light imaging, xrays), reflectivity (e.g. ultrasound, photography), proton density (e.g. MRI), concentration of radionuclides (e.g. nuclear imaging). In microscopy, intrinsic specimen absorption, em ...
... displaying it in a visual form [8]. Examples of such physical properties include absorption (e.g. light imaging, xrays), reflectivity (e.g. ultrasound, photography), proton density (e.g. MRI), concentration of radionuclides (e.g. nuclear imaging). In microscopy, intrinsic specimen absorption, em ...
Single camera spectral domain polarization
... retardance and fast-axis orientation in birefringence samples. PSOCT has been successfully applied in a variety of biomedical applications such as burn depth estimation in skin [6,7] and ophthalmology [8–10]. The recent development of PSOCT has been shifted to spectral domain implementation [11–14,1 ...
... retardance and fast-axis orientation in birefringence samples. PSOCT has been successfully applied in a variety of biomedical applications such as burn depth estimation in skin [6,7] and ophthalmology [8–10]. The recent development of PSOCT has been shifted to spectral domain implementation [11–14,1 ...
Synchronized ti scattering microscopy
... GHz sinusoid is then amplified to drive the two EO phase modulators (2PMs, EOSPACE) that form the time-lens. The intensity modulator (MZ2) is driven by a 10-MHz square wave to modulate the optical pulses for lock-in detection of the SRS signal. MZ2 replaces the external free-space modulation used in ...
... GHz sinusoid is then amplified to drive the two EO phase modulators (2PMs, EOSPACE) that form the time-lens. The intensity modulator (MZ2) is driven by a 10-MHz square wave to modulate the optical pulses for lock-in detection of the SRS signal. MZ2 replaces the external free-space modulation used in ...
Experimental studies of far-field superlens for sub-diffractional optical imaging
... demands. Recent far-field optical superlens [24, 25] and optical hyperlens [26-28] has shown exciting advancement in this direction. By designing a metamaterial lens with hyperbolic dispersion, a hyperlens is able to magnify a sub-diffraction-limited object into a diffractionlimited image which allo ...
... demands. Recent far-field optical superlens [24, 25] and optical hyperlens [26-28] has shown exciting advancement in this direction. By designing a metamaterial lens with hyperbolic dispersion, a hyperlens is able to magnify a sub-diffraction-limited object into a diffractionlimited image which allo ...
Light Scattering Spectroscopy
... • Angle: small vs. large particles • Spectrum: size and refractive index • Advantages: • Strong signal - allows use of lower cost components components. • Sensitive to important chromophores that are not fluorescent: e.g., hemoglobin. • Sensitive to both tissue structure and biochemistry. • can dist ...
... • Angle: small vs. large particles • Spectrum: size and refractive index • Advantages: • Strong signal - allows use of lower cost components components. • Sensitive to important chromophores that are not fluorescent: e.g., hemoglobin. • Sensitive to both tissue structure and biochemistry. • can dist ...
High-speed addressable confocal microscopy for functional imaging
... particular pixel, is by simultaneously scanning several sufficiently spaced spots, as is done with Nipkow disk-based scanners.8,9 By scanning ⬃20,000 fixed-size pinholes, systems based on such scanners have reported frame rates of ⬃1 kHz.10–12 However, a limitation in placing multiple excitation spo ...
... particular pixel, is by simultaneously scanning several sufficiently spaced spots, as is done with Nipkow disk-based scanners.8,9 By scanning ⬃20,000 fixed-size pinholes, systems based on such scanners have reported frame rates of ⬃1 kHz.10–12 However, a limitation in placing multiple excitation spo ...
bright field microscopy
... • Most commonly used microscopy imaging technique is bright field microscopy, where light is either passed through or reflected off a specimen • Biologists and histologists have used counter staining for over one hundred years; and this helps to differentiate the various tissues and organelles that ...
... • Most commonly used microscopy imaging technique is bright field microscopy, where light is either passed through or reflected off a specimen • Biologists and histologists have used counter staining for over one hundred years; and this helps to differentiate the various tissues and organelles that ...
Soft x-ray laser holography with wavelength resolution *
... We have generated wavelength-resolution soft x-ray holograms using a tabletop setup. The images were numerically reconstructed from a hologram recorded in the surface of a photoresist and digitized with an AFM. This method allows recording of samples without any previous preparation, as required in ...
... We have generated wavelength-resolution soft x-ray holograms using a tabletop setup. The images were numerically reconstructed from a hologram recorded in the surface of a photoresist and digitized with an AFM. This method allows recording of samples without any previous preparation, as required in ...
Adaptive Optics and the cone mosaic
... The next generation of AO is AOSLO & AO-OCT. These two new methods aim to address the original problems Williams and colleagues faced in early retinal AO. Once the subject is properly placed, wavefront correction and imaging takes place. A laser is collimated and then reflected off of a beam-splittin ...
... The next generation of AO is AOSLO & AO-OCT. These two new methods aim to address the original problems Williams and colleagues faced in early retinal AO. Once the subject is properly placed, wavefront correction and imaging takes place. A laser is collimated and then reflected off of a beam-splittin ...
Clutter elimination for deep clinical optoacoustic imaging using
... centre frequency. As a result, an imaging depth of around one centimetre or even less is typically achieved when using probes that operate in the region of 7.5 MHz [17,19,20]. Clutter can emerge from strong OA transients that are generated at the site of tissue irradiation close to the ultrasound pr ...
... centre frequency. As a result, an imaging depth of around one centimetre or even less is typically achieved when using probes that operate in the region of 7.5 MHz [17,19,20]. Clutter can emerge from strong OA transients that are generated at the site of tissue irradiation close to the ultrasound pr ...
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[pdf]
... tomography [25-28] extended to the near-field [29-43]. This scheme is particularly well suited for planar source-detection geometries that arise for example, in breast imaging. The methodology has attracted attention [29-43] because the diffusive wave modes derived using the angular-spectrum represe ...
... tomography [25-28] extended to the near-field [29-43]. This scheme is particularly well suited for planar source-detection geometries that arise for example, in breast imaging. The methodology has attracted attention [29-43] because the diffusive wave modes derived using the angular-spectrum represe ...
Get PDF - OSA Publishing
... imaging technique, dark-field (DF) microscopy is a spectacular method to form high-contrast images of unstained transparent specimens. In order to form a bright specimen image on a dark background, oblique rays from every azimuth are allowed to strike the samples, but only light scattered from the s ...
... imaging technique, dark-field (DF) microscopy is a spectacular method to form high-contrast images of unstained transparent specimens. In order to form a bright specimen image on a dark background, oblique rays from every azimuth are allowed to strike the samples, but only light scattered from the s ...
4F system 4F system with defocused input 4F system with defocused
... – Transfer function goes to zero outsize the system pass-band – Inverse transfer function will multiply the FT of the noise as well as the FT of the original signal ...
... – Transfer function goes to zero outsize the system pass-band – Inverse transfer function will multiply the FT of the noise as well as the FT of the original signal ...
Optical Microscopy Beyond the Diffraction Limit
... in glass/silica buried waveguides. We have shown that measurements of the period of the observed standing modes provides an accurate and direct measure of the effective index, which combined with the measured transverse modal shape and decay constants, determines the values of all spatial components ...
... in glass/silica buried waveguides. We have shown that measurements of the period of the observed standing modes provides an accurate and direct measure of the effective index, which combined with the measured transverse modal shape and decay constants, determines the values of all spatial components ...
Microscopy
... Turn on the fluorescence source, and rotate the reflector cube to match the stain used in your sample, typically FITC or DAPI. The source may take a few minutes to “warm up”. You should see a blue, purple or other vivid colored excitation beam. Turn off the white light halogen illumination and open ...
... Turn on the fluorescence source, and rotate the reflector cube to match the stain used in your sample, typically FITC or DAPI. The source may take a few minutes to “warm up”. You should see a blue, purple or other vivid colored excitation beam. Turn off the white light halogen illumination and open ...
Get PDF - OSA Publishing
... enhancement however is quite modest due to the low refractive index n of natural materials [4, 5]. To achieve higher resolution, a material that can support the propagation of light with higher k-vectors is needed. Metamaterials, which are artificially engineered nanocomposites possessing extraordin ...
... enhancement however is quite modest due to the low refractive index n of natural materials [4, 5]. To achieve higher resolution, a material that can support the propagation of light with higher k-vectors is needed. Metamaterials, which are artificially engineered nanocomposites possessing extraordin ...
The Ultrasound Machine
... The pulse generator produces the electrical pulses that are applied to the transducer. For conventional ultrasound imaging the pulses are produced at a rate of approximately 1,000 pulses per second. This is the pulse rate (pulses per second) and not the frequency which is the number of cycles or vib ...
... The pulse generator produces the electrical pulses that are applied to the transducer. For conventional ultrasound imaging the pulses are produced at a rate of approximately 1,000 pulses per second. This is the pulse rate (pulses per second) and not the frequency which is the number of cycles or vib ...
White-light diffraction tomography of unlabelled live cells
... Here, we report on a new approach for label-free tomography of live cells and other transparent specimens, which we refer to as white-light diffraction tomography (WDT). WDT offers a high-performance, simple design, as well as suitability for operation in a conventional microscopy setting. Its main ...
... Here, we report on a new approach for label-free tomography of live cells and other transparent specimens, which we refer to as white-light diffraction tomography (WDT). WDT offers a high-performance, simple design, as well as suitability for operation in a conventional microscopy setting. Its main ...
Inleiding Optica 2010
... -If you can take it apart you should be able to put it back together -Do unto others as you would have them do to you ...
... -If you can take it apart you should be able to put it back together -Do unto others as you would have them do to you ...