An In Vitro Model for Neuroscience: Differentiation of SH
... Neuroscience, including research on Alzheimer´s disease, is hampered by the lack of suitable in vitro models to study the human nervous system. To counteract this, many attempts to differentiate cell lines into more neuron-like cells have been performed, resulting in partial expression of neuronal f ...
... Neuroscience, including research on Alzheimer´s disease, is hampered by the lack of suitable in vitro models to study the human nervous system. To counteract this, many attempts to differentiate cell lines into more neuron-like cells have been performed, resulting in partial expression of neuronal f ...
Progressive lineage analysis by cell sorting and culture identifies
... formation of the embryoid body With the surface markers described above, we next investigated how far the specification of ES cells reaches in the absence of the embryoid body structure. In a series of preliminary experiments, we compared dishes coated with gelatin, fibronectin, type I collagen, or ...
... formation of the embryoid body With the surface markers described above, we next investigated how far the specification of ES cells reaches in the absence of the embryoid body structure. In a series of preliminary experiments, we compared dishes coated with gelatin, fibronectin, type I collagen, or ...
traffic jams affect plant development and signal transduction
... pathways overlap and share common components. The amino-acid sequences of protein families that mediate protein trafficking are conserved in plants, animals and yeast; however, it is not possible to predict their functions solely on the basis of sequence similarity. The secretory pathway has many un ...
... pathways overlap and share common components. The amino-acid sequences of protein families that mediate protein trafficking are conserved in plants, animals and yeast; however, it is not possible to predict their functions solely on the basis of sequence similarity. The secretory pathway has many un ...
PDF
... can give rise to mesodermal and neural progenitors of different posterior axial levels (Brown and Storey, 2000; Cambray and Wilson, 2002; Tzouanacou et al., 2009; Tsakiridis et al., 2014). These cells, named long-term axial neuromesodermal precursors (NMp), can be identified because they co-express ...
... can give rise to mesodermal and neural progenitors of different posterior axial levels (Brown and Storey, 2000; Cambray and Wilson, 2002; Tzouanacou et al., 2009; Tsakiridis et al., 2014). These cells, named long-term axial neuromesodermal precursors (NMp), can be identified because they co-express ...
Localization and characterization of major histocompatibility
... Purpose. To identify potential antigen-presenting cells in the choroid and retina of the normal rat eye, with a view to proposing a role for such cells in the induction and perpetuation of experimental autoimmune uveoretinitis, a model of human uveoretinal inflammation. Methods. Immunohistochcinical ...
... Purpose. To identify potential antigen-presenting cells in the choroid and retina of the normal rat eye, with a view to proposing a role for such cells in the induction and perpetuation of experimental autoimmune uveoretinitis, a model of human uveoretinal inflammation. Methods. Immunohistochcinical ...
Systematic profiling of cellular phenotypes with spotted cell
... affecting the pheromone-response pathway, either directly or indirectly, could be identified by examining shmoo phenotypes when the deletion collection was treated with alpha factor. We treated the entire mating type a haploid yeast deletion collection with alpha factor, then constructed and imaged ...
... affecting the pheromone-response pathway, either directly or indirectly, could be identified by examining shmoo phenotypes when the deletion collection was treated with alpha factor. We treated the entire mating type a haploid yeast deletion collection with alpha factor, then constructed and imaged ...
Compartments Target the Antigen to Distinct Intracellular B Cell
... ecules are present throughout the endocytic pathway (9). The addressing of neosynthesized class II molecules is monitored by their association with the invariant chain (Ii), which chaperons the newly formed complex to the endocytic pathway (10 –12). Once in the endocytic compartments, the invariant ...
... ecules are present throughout the endocytic pathway (9). The addressing of neosynthesized class II molecules is monitored by their association with the invariant chain (Ii), which chaperons the newly formed complex to the endocytic pathway (10 –12). Once in the endocytic compartments, the invariant ...
Epithelial cell adhesion molecule-targeted drug delivery for cancer
... aspects of EpCAM-targeted drug delivery for cancer therapy. The authors discuss seminal findings, which distinguish EpCAM as a target with oncogenic function and abundant expression in epithelial tumors. Moreover, recent trends in engineering improved anti-EpCAM antibodies, binding proteins that are ...
... aspects of EpCAM-targeted drug delivery for cancer therapy. The authors discuss seminal findings, which distinguish EpCAM as a target with oncogenic function and abundant expression in epithelial tumors. Moreover, recent trends in engineering improved anti-EpCAM antibodies, binding proteins that are ...
Comparison of cryofixation and aldehyde fixation for plant actin
... (E, F, I, J), and embedded in Steedman’s wax. Formaldehyde-fixed samples show identical actin staining patterns to those obtained in cryofixed samples. Shown are cells of cortex (A–F), epidermis (G, I), and stele (H, J). Photographs were taken using an epifluorescence microscope. Scale bar in J = 10 ...
... (E, F, I, J), and embedded in Steedman’s wax. Formaldehyde-fixed samples show identical actin staining patterns to those obtained in cryofixed samples. Shown are cells of cortex (A–F), epidermis (G, I), and stele (H, J). Photographs were taken using an epifluorescence microscope. Scale bar in J = 10 ...
Phenotypic Mixing of Retroviruses in Mitogen
... higher than those observed in BALB/c cultures (Table 1). Thus it appears that NFS/N cells also produce a defective retrovirus which can be distinguished from the 129 virus (Jongstra & Moroni, 198 l) by the presence of reverse transcriptase. BUdR treatment appeared to inhibit the production of this v ...
... higher than those observed in BALB/c cultures (Table 1). Thus it appears that NFS/N cells also produce a defective retrovirus which can be distinguished from the 129 virus (Jongstra & Moroni, 198 l) by the presence of reverse transcriptase. BUdR treatment appeared to inhibit the production of this v ...
bundle sheath defective, a mutation that disrupts cellular
... 1988b). In dark-grown tissues, both BS and M cells accumulate RuBPCase although the leaves are non-photosynthetic (Sheen and Bogorad, 1986; Langdale et al., 1988b). These results suggest that maize develops a C3 pattern of cell-type differentiation (RuBPCase in all photosynthetic cells) by default a ...
... 1988b). In dark-grown tissues, both BS and M cells accumulate RuBPCase although the leaves are non-photosynthetic (Sheen and Bogorad, 1986; Langdale et al., 1988b). These results suggest that maize develops a C3 pattern of cell-type differentiation (RuBPCase in all photosynthetic cells) by default a ...
Vacuole Membrane Protein 1 Is an Endoplasmic
... line Caki-2, and it was found to be essential for cell– cell contact (Sauermann et al., 2008). These results suggested a totally different function of Vmp1 in tumor cells. Therefore, the function of this protein remains controversial and seems to depend on the specific cell type studied. Vmp1 is a c ...
... line Caki-2, and it was found to be essential for cell– cell contact (Sauermann et al., 2008). These results suggested a totally different function of Vmp1 in tumor cells. Therefore, the function of this protein remains controversial and seems to depend on the specific cell type studied. Vmp1 is a c ...
Systems biology of virus-host signaling network
... infected and uninfected monocytes, HIV-1 infection significantly changed monocyte activation in response to granulocyte-macrophage colony-stimulating factor (GM-CSF) via down-regulation of Jak/STAT signaling but enhancement of MAPK signaling (22), resulting in defective antigen presentation. These s ...
... infected and uninfected monocytes, HIV-1 infection significantly changed monocyte activation in response to granulocyte-macrophage colony-stimulating factor (GM-CSF) via down-regulation of Jak/STAT signaling but enhancement of MAPK signaling (22), resulting in defective antigen presentation. These s ...
Inactivation of S-adenosylhomocysteine hydrolase intact cells
... pronounced in the hepatocytes (frcrn 50 to 3500 prol/l0 cells after 3 hours of exposure to ara-A). The cellular content of AdoMet increases slightly in liver cells, but remains lDlchanged in the cultured cells. Accumulation of intracellular MoHey leads to a marked egress of AdoHcy from the cells. Af ...
... pronounced in the hepatocytes (frcrn 50 to 3500 prol/l0 cells after 3 hours of exposure to ara-A). The cellular content of AdoMet increases slightly in liver cells, but remains lDlchanged in the cultured cells. Accumulation of intracellular MoHey leads to a marked egress of AdoHcy from the cells. Af ...
chapter07_section02_JKedit
... Cells that are active in protein synthesis are often packed with ribosomes. ...
... Cells that are active in protein synthesis are often packed with ribosomes. ...
Induction of Mac-2BP by nerve growth factor is
... pathways. This also results in the activation of several transcriptional factors (18, 19) including NF-κB (20). NGF activates NF-κB and AP-1 in sympathetic neurons, and NGF-inducible NF-κB is required for neuronal survival (21). We report here that two NF-κB binding sites on the Mac-2BP promoter reg ...
... pathways. This also results in the activation of several transcriptional factors (18, 19) including NF-κB (20). NGF activates NF-κB and AP-1 in sympathetic neurons, and NGF-inducible NF-κB is required for neuronal survival (21). We report here that two NF-κB binding sites on the Mac-2BP promoter reg ...
emboj2011361-sup
... 2 hours. For induction of the crg-promoter in strains AB33Mcs1G3_rKin1rigor AB33 G3Dyn2_ Kin1 rigor, AB33 G3Myo5_ Kin1 rigor and AB33Mcs1G3_rMyo5rigor, cells were grown in CM-glucose medium to an OD600 ≈ 0.5 and transferred into CM-medium containing 1% (w/v) arabinose as sole carbon source (CMarabin ...
... 2 hours. For induction of the crg-promoter in strains AB33Mcs1G3_rKin1rigor AB33 G3Dyn2_ Kin1 rigor, AB33 G3Myo5_ Kin1 rigor and AB33Mcs1G3_rMyo5rigor, cells were grown in CM-glucose medium to an OD600 ≈ 0.5 and transferred into CM-medium containing 1% (w/v) arabinose as sole carbon source (CMarabin ...
CELL
... • Protists - the ancestors of plantsanimals and fungi. • They may have been around as long as two billion years. • A protist is an organism made of a single cell - yet it can live- eatand reproduce like other living things. • One of the most fascinating protists is the amoeba. • Amoebas- like a ...
... • Protists - the ancestors of plantsanimals and fungi. • They may have been around as long as two billion years. • A protist is an organism made of a single cell - yet it can live- eatand reproduce like other living things. • One of the most fascinating protists is the amoeba. • Amoebas- like a ...
Collagen Binding Proteins Derived from the Embryonic Fibroblast
... surface-ECM binding was a major advance in the understanding of substrate adhesion (Pierschbacher and Ruoslahti, 1984). The RGD receptors comprise a superfamily of translation products termed the Integrins (Hynes, 1987; Buck and Horwitz, 1987; Ruoslahti and Pierschbacher, 1987). In some instances, r ...
... surface-ECM binding was a major advance in the understanding of substrate adhesion (Pierschbacher and Ruoslahti, 1984). The RGD receptors comprise a superfamily of translation products termed the Integrins (Hynes, 1987; Buck and Horwitz, 1987; Ruoslahti and Pierschbacher, 1987). In some instances, r ...
Gene expression analysis uncovers similarity and differences
... mediastinal B-cell lymphomas, 13 HIV-related DLBCL, and 34 marginal zone lymphomas (MZLs). In addition, we analyzed 20 samples of normal B-cell subpopulations, including GC (n ⫽ 10), naive (n ⫽ 5), and memory (n ⫽ 5) cells. Details on such cases are provided in supplemental File 1 (available on the ...
... mediastinal B-cell lymphomas, 13 HIV-related DLBCL, and 34 marginal zone lymphomas (MZLs). In addition, we analyzed 20 samples of normal B-cell subpopulations, including GC (n ⫽ 10), naive (n ⫽ 5), and memory (n ⫽ 5) cells. Details on such cases are provided in supplemental File 1 (available on the ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.