Supplementary Figures 1–2 and Table (ppt 202K)
... We addressed how the drugs we used to block steps within the calcium flux machinery and which in turn inhibited engulfment correlated with calcium flux during engulfment. As above, we measured cellar calcium levels using the fluorimetry based assay that assesses flux in a whole population of cells i ...
... We addressed how the drugs we used to block steps within the calcium flux machinery and which in turn inhibited engulfment correlated with calcium flux during engulfment. As above, we measured cellar calcium levels using the fluorimetry based assay that assesses flux in a whole population of cells i ...
DISTINCT RECOGNITION PHENOTYPES EXIST FOR T CELL
... It has been difficult to choose between the two major possibilities that may account for this limitation. Strong arguments (6) have been advanced in favor of MHC-determined gaps in the available peripheral repertoire, as well as for determinant selection mechanisms of antigen presentation necessary ...
... It has been difficult to choose between the two major possibilities that may account for this limitation. Strong arguments (6) have been advanced in favor of MHC-determined gaps in the available peripheral repertoire, as well as for determinant selection mechanisms of antigen presentation necessary ...
CDK11p58 kinase activity is required to protect sister chromatid
... expressing an EGFP-CDK11p58 construct. The cells were transfected with luciferase siRNA (as a negative control) or a CDK11 siRNA (5’AGCGGCUGAAGAUGGAGAA-3’) that targets the endogenous CDK11 mRNA but not the GFP-CDK11 mRNA using Hiperfect (Qiagen) as a transfection reagent. Immunofluorescence was per ...
... expressing an EGFP-CDK11p58 construct. The cells were transfected with luciferase siRNA (as a negative control) or a CDK11 siRNA (5’AGCGGCUGAAGAUGGAGAA-3’) that targets the endogenous CDK11 mRNA but not the GFP-CDK11 mRNA using Hiperfect (Qiagen) as a transfection reagent. Immunofluorescence was per ...
3-4 Teacher
... Mice have been produced with human genes that make their immune systems act similarly to those of humans. This allows scientists to study the effects of diseases on the human immune system. ...
... Mice have been produced with human genes that make their immune systems act similarly to those of humans. This allows scientists to study the effects of diseases on the human immune system. ...
Kingdom Monera 1_1_
... The are all prokaryotic. This means that their cells lack a nucleus and other membrane bound organelles. They are all unicellular, though may form clumps, strands, chains or even layers and colonies. They are asexual. They reproduce by binary fission. ...
... The are all prokaryotic. This means that their cells lack a nucleus and other membrane bound organelles. They are all unicellular, though may form clumps, strands, chains or even layers and colonies. They are asexual. They reproduce by binary fission. ...
Cell Structure and Function
... Cell Size At 0.1–5.0 µm in diameter, prokaryotic cells are significantly smaller than eukaryotic cells, which have diameters ranging from 10–100 µm (Figure 3.6). The small size of prokaryotes allows ions and organic molecules that enter them to quickly spread to other parts of the cell. Similarly, a ...
... Cell Size At 0.1–5.0 µm in diameter, prokaryotic cells are significantly smaller than eukaryotic cells, which have diameters ranging from 10–100 µm (Figure 3.6). The small size of prokaryotes allows ions and organic molecules that enter them to quickly spread to other parts of the cell. Similarly, a ...
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... Bloom's Taxonomy: Application/Analysis Section: 6.6 53) The cell walls of bacteria, fungi, and plant cells and the extracellular matrix of animal cells are all external to the plasma membrane. Which of the following is a characteristic common to all of these extracellular structures? A) They must bl ...
... Bloom's Taxonomy: Application/Analysis Section: 6.6 53) The cell walls of bacteria, fungi, and plant cells and the extracellular matrix of animal cells are all external to the plasma membrane. Which of the following is a characteristic common to all of these extracellular structures? A) They must bl ...
Network structure determines patterns of network
... We create the initial networks prior to neurogenesis by placing 1000 excitatory neurons and either 200 (normal conditions) or 100 (reduced inhibition) inhibitory neurons at random on a two-dimensional square lattice with 40 lattice sites per side and periodic boundaries. A lattice site can hold at m ...
... We create the initial networks prior to neurogenesis by placing 1000 excitatory neurons and either 200 (normal conditions) or 100 (reduced inhibition) inhibitory neurons at random on a two-dimensional square lattice with 40 lattice sites per side and periodic boundaries. A lattice site can hold at m ...
Stimulated release of fluorescently labeled IgE fragments that
... DNP-BSA in the presence of 3 µM cytochalasin D for 1 hour at 37°C. The cells were maintained in suspension by continual rotation, and an identical cell sample with 3 µM cytochalasin D in the absence of antigen was rotated in parallel as a control. The cells were sedimented by centrifuging for 10 min ...
... DNP-BSA in the presence of 3 µM cytochalasin D for 1 hour at 37°C. The cells were maintained in suspension by continual rotation, and an identical cell sample with 3 µM cytochalasin D in the absence of antigen was rotated in parallel as a control. The cells were sedimented by centrifuging for 10 min ...
Alleati per un`antropologia positiva Scienza e Vita in dialogo con AIOM
... tumor angiogenesis are logical targets for pharmacological manipulation given the important role they play in the growth and development of cancers • A lack of validated biomarkers and patient screening restricts our ability to tailor specific drugs to patient cohorts, and might be seen as one of th ...
... tumor angiogenesis are logical targets for pharmacological manipulation given the important role they play in the growth and development of cancers • A lack of validated biomarkers and patient screening restricts our ability to tailor specific drugs to patient cohorts, and might be seen as one of th ...
Biology of Tooth Movement
... of Arachidonic acid, were originally demonstrated in leukocytes and were called leukotrienes. It is possible then, since prostaglandins are not fully responsible for bone remodeling associated with tooth movement, lipoxygenase products may also be involved. A rat orthodontic model has been used to d ...
... of Arachidonic acid, were originally demonstrated in leukocytes and were called leukotrienes. It is possible then, since prostaglandins are not fully responsible for bone remodeling associated with tooth movement, lipoxygenase products may also be involved. A rat orthodontic model has been used to d ...
Head segmentation
... mandibular arch and more anterior structures, cells from rhombomeres 3-5 go into the hyoid arch, and cells from more posterior rhombomeres go into the gill arches (figure from Kuratani et al. 2001). ...
... mandibular arch and more anterior structures, cells from rhombomeres 3-5 go into the hyoid arch, and cells from more posterior rhombomeres go into the gill arches (figure from Kuratani et al. 2001). ...
Plasmacytoid Dendritic Cells Induce Plasma Cell Differentiation
... 3A, the addition of 5000 pDCs to CD40-activated B cells cultured with Flu virus resulted in a 10-fold increase of IgG production (12 replicates of six independent experiments, p ⬍ 0.0001). As few as 1000 pDCs suffice for significant enhancement of IgG secretion (Figure 3B). However, in the absence o ...
... 3A, the addition of 5000 pDCs to CD40-activated B cells cultured with Flu virus resulted in a 10-fold increase of IgG production (12 replicates of six independent experiments, p ⬍ 0.0001). As few as 1000 pDCs suffice for significant enhancement of IgG secretion (Figure 3B). However, in the absence o ...
Organization of Yeast Communities Cell Signals, Cell
... pathogenic (17, 45), but the relationship between mature biofilms and virulence may not be a simple one, since biofilms formed by several other pathogenic Candida species may also lack the mature structures formed by C. albicans (30). Visibly organized communities. One striking type of colony organi ...
... pathogenic (17, 45), but the relationship between mature biofilms and virulence may not be a simple one, since biofilms formed by several other pathogenic Candida species may also lack the mature structures formed by C. albicans (30). Visibly organized communities. One striking type of colony organi ...
The role of vacuole in plant cell death
... Non-Destructive versus Destructive Vacuole-mediated Cell Death As plants do not have mobile immune cells, they have evolved unique immune systems with different defense strategies for different pathogens.18 One of these strategies is the hypersensitive response (HR), which confers broad-spectrum dis ...
... Non-Destructive versus Destructive Vacuole-mediated Cell Death As plants do not have mobile immune cells, they have evolved unique immune systems with different defense strategies for different pathogens.18 One of these strategies is the hypersensitive response (HR), which confers broad-spectrum dis ...
d-glucose-sensitive neurosecretory cells of the crab cancer borealis
... to glucose. This response was always hyperpolarizing in normal saline, highly selective for D-glucose in the concentration range found in the hemolymph of living animals and was carried by K+. However, the glucose-regulated K+ conductance does not appear to be an ATP-sensitive K+ channel and thus ma ...
... to glucose. This response was always hyperpolarizing in normal saline, highly selective for D-glucose in the concentration range found in the hemolymph of living animals and was carried by K+. However, the glucose-regulated K+ conductance does not appear to be an ATP-sensitive K+ channel and thus ma ...
Mutations in a novel gene, myoblast city, provide evidence
... resembles the pattern of wild-type muscle precursors, with a To explore this idea, we stained mbc mutant embryos using segmentally repeated arrangement of ventral, lateral and dorsal antibodies against S59 and VG, the products of two genes that cell clusters (Fig. 1B). Strikingly, with only a few ex ...
... resembles the pattern of wild-type muscle precursors, with a To explore this idea, we stained mbc mutant embryos using segmentally repeated arrangement of ventral, lateral and dorsal antibodies against S59 and VG, the products of two genes that cell clusters (Fig. 1B). Strikingly, with only a few ex ...
PHAGOCYTOSIS AND INTRACELLULAR KILLING OF SERUM
... Intracellular killing of S. aureus by FcγRIIaexpressing cells was determined as described (21) with minor modifications. In short, equal volumes of 1x107 cells/ml and 5x107 opsonized bacteria/ml of HBSS-gel were mixed and then incubated for 90 minutes at 37°C under slow rotation. Phagocytosis was st ...
... Intracellular killing of S. aureus by FcγRIIaexpressing cells was determined as described (21) with minor modifications. In short, equal volumes of 1x107 cells/ml and 5x107 opsonized bacteria/ml of HBSS-gel were mixed and then incubated for 90 minutes at 37°C under slow rotation. Phagocytosis was st ...
MOLECULAR PROFILE AND CELL CYCLE IN MCF-7 AND MCF
... form of doxorubicin in sensitive MCF-7 cells. According to the data from numerous clinical observations [10], the absence of steroid receptor expression found in ⅓ of BC patients correlates with low sensitivity of tumors to anti-tumor therapy. Earlier we have shown that in MCF-7 cells resistant to ...
... form of doxorubicin in sensitive MCF-7 cells. According to the data from numerous clinical observations [10], the absence of steroid receptor expression found in ⅓ of BC patients correlates with low sensitivity of tumors to anti-tumor therapy. Earlier we have shown that in MCF-7 cells resistant to ...
Altered Behaviour of Erythrocytes in Scleroderma
... t (A) Ascending, (D) descending boundaries. ...
... t (A) Ascending, (D) descending boundaries. ...
Fulltext PDF - Indian Academy of Sciences
... exist at all levels of biology, ranging from gene expression to complex behaviours. They are controlled by a cellular clock that has been observed in organisms from all phyla. The underlying molecular mechanism has been described using genetic strategies that identified a set of clock genes that fun ...
... exist at all levels of biology, ranging from gene expression to complex behaviours. They are controlled by a cellular clock that has been observed in organisms from all phyla. The underlying molecular mechanism has been described using genetic strategies that identified a set of clock genes that fun ...
Inflammation and fever
... In addition, the body has the capacity to respond to both minor injuries such as bruising, scratching, cuts, and abrasions, as well as to major injuries such as severe burns and amputation of limbs. Depending on the severity of the tissue damage resulting from an injury, the integrity of the skin or ...
... In addition, the body has the capacity to respond to both minor injuries such as bruising, scratching, cuts, and abrasions, as well as to major injuries such as severe burns and amputation of limbs. Depending on the severity of the tissue damage resulting from an injury, the integrity of the skin or ...
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... to ensure that the observed effect was not due to the fungistatic actions of the metal complex. When cells are pre-grown in sub-MIC90 levels of the silver or copper complexes and then assessed for their response to miconazole or amphotericin B there is evidence of alterations in susceptibility. Pre- ...
... to ensure that the observed effect was not due to the fungistatic actions of the metal complex. When cells are pre-grown in sub-MIC90 levels of the silver or copper complexes and then assessed for their response to miconazole or amphotericin B there is evidence of alterations in susceptibility. Pre- ...
Loosening of plant cell walls by expansins
... ‘wall loosening’, a general term that does not specify the exact mechanism for induction of cell wall extension. Expansins, proteins with relative molecular mass of about 26,000, were isolated first from young cucumber seedlings and subsequently from other plant tissues, where their activity was ass ...
... ‘wall loosening’, a general term that does not specify the exact mechanism for induction of cell wall extension. Expansins, proteins with relative molecular mass of about 26,000, were isolated first from young cucumber seedlings and subsequently from other plant tissues, where their activity was ass ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.