Adeno-Associated Virus Vectors for Gene Therapy - diss.fu
... Rep68 and Rep40, are translated from spliced mRNAs (Fig. 1). Since the site-specific DNA-binding and the endonuclease domains are located within the N-terminus of the Rep78/68 proteins these protein characteristics are absent from Rep52/40. However, all Rep proteins possess a 3’-to-5’ helicase activ ...
... Rep68 and Rep40, are translated from spliced mRNAs (Fig. 1). Since the site-specific DNA-binding and the endonuclease domains are located within the N-terminus of the Rep78/68 proteins these protein characteristics are absent from Rep52/40. However, all Rep proteins possess a 3’-to-5’ helicase activ ...
Ectopic Expression of AINTEGUMENTA in Arabidopsis Plants
... this construct was transformed into wild-type Arabidopsis plants. As shown in Figure 1E–H, 35S::ANT confers persistent ANT expression in developing floral organs. Approximately one-half of the transgenic plants (66 out of 127 plants) produced larger than normal flowers (Fig. 2A). Of the other transf ...
... this construct was transformed into wild-type Arabidopsis plants. As shown in Figure 1E–H, 35S::ANT confers persistent ANT expression in developing floral organs. Approximately one-half of the transgenic plants (66 out of 127 plants) produced larger than normal flowers (Fig. 2A). Of the other transf ...
The Role Of The Planar Cell Polarity Pathway In The Second Heart
... Outflow Tract (OFT) malformations underlie a majority of congenital heart defects (CHD) in humans and are a leading cause of childhood mortality. The OFT, which gives rise to the aorta and pulmonary artery of the heart, relies on the contribution of the Second Heart Field (SHF) progenitors in the ph ...
... Outflow Tract (OFT) malformations underlie a majority of congenital heart defects (CHD) in humans and are a leading cause of childhood mortality. The OFT, which gives rise to the aorta and pulmonary artery of the heart, relies on the contribution of the Second Heart Field (SHF) progenitors in the ph ...
Purification and Characterization of Extracellular and
... The agar diffusion method (Somers & Bevan, 1969) was used for the quantitative determination of killer toxin activity. The diameter (D) of the inhibition zone was proportional to the logarithm of the concentration (log C) of killer toxin for toxin concentrations within the range 0-001-7.0 pg ml-' ;b ...
... The agar diffusion method (Somers & Bevan, 1969) was used for the quantitative determination of killer toxin activity. The diameter (D) of the inhibition zone was proportional to the logarithm of the concentration (log C) of killer toxin for toxin concentrations within the range 0-001-7.0 pg ml-' ;b ...
Sucrose metabolism: regulatory mechanisms and pivotal roles in
... In addition to their production of metabolic substrates, each site and path of sucrose cleavage described above can initiate a distinctive profile of sugar signals, which in turn can have profound developmental effects (Figure 3). In general, hexoses favor cell division and expansion, whereas sucros ...
... In addition to their production of metabolic substrates, each site and path of sucrose cleavage described above can initiate a distinctive profile of sugar signals, which in turn can have profound developmental effects (Figure 3). In general, hexoses favor cell division and expansion, whereas sucros ...
PIN-Dependent Auxin Transport: Action, Regulation
... nondirectional stream in the phloem along with photosynthetic assimilates, and second, slow and directional cell-to-cell polar auxin transport (PAT). While phloem transport provides a general way to deliver auxin from the sites of its synthesis (mostly in young leaves) to recipient organs, PAT distr ...
... nondirectional stream in the phloem along with photosynthetic assimilates, and second, slow and directional cell-to-cell polar auxin transport (PAT). While phloem transport provides a general way to deliver auxin from the sites of its synthesis (mostly in young leaves) to recipient organs, PAT distr ...
Airway surface liquid pH in well-differentiated airway - AJP-Cell
... intratracheal instillation of 4.9 g of glibenclamide in perfluorocarbon. To create acute metabolic acidosis or alkalosis, HCl (0.5 meq H⫹) or NaHCO3 (0.3 meq) were injected intraperitoneally. ASL pH was measured after 20 min. To create respiratory acidosis or alkalosis, the upper trachea of anesthe ...
... intratracheal instillation of 4.9 g of glibenclamide in perfluorocarbon. To create acute metabolic acidosis or alkalosis, HCl (0.5 meq H⫹) or NaHCO3 (0.3 meq) were injected intraperitoneally. ASL pH was measured after 20 min. To create respiratory acidosis or alkalosis, the upper trachea of anesthe ...
Class II Histone Deacetylases Are Directly Recruited by BCL6
... genes has been identified by DNA microarray screening in lymphocytes. BCL6 was found to repress a number of genes involved in B cell activation and terminal differentiation, inflammation, and cell cycle regulation (12). Earlier studies have shaded light on the molecular mechanisms by which BCL6 nega ...
... genes has been identified by DNA microarray screening in lymphocytes. BCL6 was found to repress a number of genes involved in B cell activation and terminal differentiation, inflammation, and cell cycle regulation (12). Earlier studies have shaded light on the molecular mechanisms by which BCL6 nega ...
Vesicles versus Tubes: is ER-Golgi Transport in
... sciences. Indeed, and with few exceptions (e.g. Ito et al., 2014) there are few reviews ...
... sciences. Indeed, and with few exceptions (e.g. Ito et al., 2014) there are few reviews ...
NIH Public Access - Khademhosseini Lab
... will discuss the different types of nanocomposite hydrogels and highlight some of the emerging biomedical and biotechnological applications of these hydrogels. In particular, the emerging trends in designing complex hydrogels with multiple functionalities are discussed. We will also evaluate the nan ...
... will discuss the different types of nanocomposite hydrogels and highlight some of the emerging biomedical and biotechnological applications of these hydrogels. In particular, the emerging trends in designing complex hydrogels with multiple functionalities are discussed. We will also evaluate the nan ...
Auxin coordinates cell division and cell fate specification during
... when and where lateral roots are initiated have a high degree of plasticity, integrating both intrinsic and extrinsic levels of control. Environmental factors, such as water, nutrients, and temperature have significant influences on the root architecture (Dubrovsky and Rost, 2003). The most importan ...
... when and where lateral roots are initiated have a high degree of plasticity, integrating both intrinsic and extrinsic levels of control. Environmental factors, such as water, nutrients, and temperature have significant influences on the root architecture (Dubrovsky and Rost, 2003). The most importan ...
Physiology and molecular biology of petal senescence
... both of some ethylene-sensitive plants (Ipomoea; Petunia) and of an ethylene-insensitive plant (Argyranthemum). Other organelles also disappear and the volume of cytoplasm becomes very low, leaving only a small strip next to the cell wall. At least some mitochondria, and in carnation some plastids, ...
... both of some ethylene-sensitive plants (Ipomoea; Petunia) and of an ethylene-insensitive plant (Argyranthemum). Other organelles also disappear and the volume of cytoplasm becomes very low, leaving only a small strip next to the cell wall. At least some mitochondria, and in carnation some plastids, ...
Downloaded - The Journal of Cell Biology
... deleterious effects on muscle repair, thus precluding direct inactivation of TGF-1 as a therapeutic option for combating fibrosis in DMD. Therefore, it becomes clinically relevant to identify more specific targets within the TGF-1 profibrotic pathway in dystrophic muscle. TGF-1 is secreted as a l ...
... deleterious effects on muscle repair, thus precluding direct inactivation of TGF-1 as a therapeutic option for combating fibrosis in DMD. Therefore, it becomes clinically relevant to identify more specific targets within the TGF-1 profibrotic pathway in dystrophic muscle. TGF-1 is secreted as a l ...
NUBBIN and JAGGED define stamen and carpel
... Cell layer number and cell width measurements were determined from the same transverse cross-sections of stage-13 gynoecia. For each genotype, 16 sections were imaged from four gynoecia. Sections were taken from the approximate middle of the gynoecium and were separated by at least 30 m from each o ...
... Cell layer number and cell width measurements were determined from the same transverse cross-sections of stage-13 gynoecia. For each genotype, 16 sections were imaged from four gynoecia. Sections were taken from the approximate middle of the gynoecium and were separated by at least 30 m from each o ...
Formation of a highly peptide-receptive state of class II MHC.
... of Sadegh-Nasseri et al. (1994), our measurements used a lower peptide concentration, high performance size exclusion chromatography instead of spin columns, and HA peptide labeled with carboxyfluorescein on the N terminus instead of with 125I on the P1 tyrosine residue. This tyrosine is the most im ...
... of Sadegh-Nasseri et al. (1994), our measurements used a lower peptide concentration, high performance size exclusion chromatography instead of spin columns, and HA peptide labeled with carboxyfluorescein on the N terminus instead of with 125I on the P1 tyrosine residue. This tyrosine is the most im ...
Altered morphology produced by ftsZ expression in
... tuberculosis (Dziadek et al., 2002), among others. In N. gonorrhoeae, the expression of FtsZ resulted in abnormal cell division in some cells, characterized by the presence of multiple and atypically arranged cell division sites. H. salinarium transformed with a plasmid carrying its own ftsZ gene yi ...
... tuberculosis (Dziadek et al., 2002), among others. In N. gonorrhoeae, the expression of FtsZ resulted in abnormal cell division in some cells, characterized by the presence of multiple and atypically arranged cell division sites. H. salinarium transformed with a plasmid carrying its own ftsZ gene yi ...
Self-Incompatibility-induced Programmed Cell
... Dangl, 1996; van Doorn, 2011; van Doorn et al., 2011). There is considerable biochemical evidence for the involvement of caspase-like activities in plant PCD (van Doorn and Woltering, 2005). For example, Vacuolar Processing Enzyme (VPE) has YVADase (caspase-1-like) activity (Hatsugai et al., 2004; R ...
... Dangl, 1996; van Doorn, 2011; van Doorn et al., 2011). There is considerable biochemical evidence for the involvement of caspase-like activities in plant PCD (van Doorn and Woltering, 2005). For example, Vacuolar Processing Enzyme (VPE) has YVADase (caspase-1-like) activity (Hatsugai et al., 2004; R ...
Biology - Cellular, Molecular, Professional Emphasis (sample 4 Year Plan) 2011.doc
... Bio 341 Immunology (F) (3 cr) (if not used to fill preceding requirement) Bio 350 Electron Microscopy (F) (4 cr) (if not used to fill preceding requirement) Bio 354 Parasitology (S) (3 cr) Bio 374 Cell Immunology (3cr) (if not used to fill preceding requirement) Bio 389 Biotechnology (S) (3 cr) (if ...
... Bio 341 Immunology (F) (3 cr) (if not used to fill preceding requirement) Bio 350 Electron Microscopy (F) (4 cr) (if not used to fill preceding requirement) Bio 354 Parasitology (S) (3 cr) Bio 374 Cell Immunology (3cr) (if not used to fill preceding requirement) Bio 389 Biotechnology (S) (3 cr) (if ...
Extracellular matrix
In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).