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APBiology 12
APBiology 12

... Concept 20.2 DNA technology allows us to study the sequence, expression, and function of a gene.  Once scientists have prepared homogeneous samples of DNA, each containing a large number of identical segments, they can ask some interesting questions about specific genes and their functions. o Does ...
Specialized techniques for site-directed mutagenesis in cyanobacteria
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... reading frame can exert polar effects on downstream genes. Additionally, when several genes within the same strain are to be inactivated, the use of many different antibiotics to select for various insertions can have cumulative, detrimental effects on cell culture viability. In light of these compl ...
Campbell Chapter 23 - California Science Teacher
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... better or more common. There are, for example, quite a few human genetic diseases, some lethal, caused by dominant alleles. The terms dominant and recessive simply mean that if two alleles in this relationship are in a heterozygous genotype, the action of the dominant will be expressed and the actio ...
DNA Sequence Analysis
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Mitosis and Cell Cycle
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... • For
example,
in
mice
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many
other mammals,
coat
color
depends
on
two
genes – One
gene
determines
the
pigment
color
(with alleles
B
for
black
and
b
for
brown) – The
other
gene
(with
alleles
C
for
color
and
c
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color)
determines
whether
the
pigment
will be
deposited
in
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hair ...
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Population Genetics

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Title of Unit: DNA, Genetics and Biotechnology Course and Grade

... Cellular genetics, Describe the method and  steps in structure and function of Mendel's true breeding experiments DNA in cells, genetic    State the two laws of heredity developed mechanisms and from Mendel's work inheritance, mutation and c.    Describe how Mendel's work can now be modern genetics ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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