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Improvement of GSH production by metabolic engineering the
Improvement of GSH production by metabolic engineering the

... fragment conjugating the S. cerevisiae phosphoglycerate kinase (PGK) promoter gene, S. cerevisiae γ-GC synthetase gene, and S. cerevisiae PGK terminator gene was obtained from pGK402GCS (Yoshida et al. 2011) by digestion with XhoI and NotI. The digested fragment was inserted into the SalI / NotI sit ...
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Evolution of mouse globin superfamily

... organisms appeared > 2 billion years after cellular evolution ...
Barbara McClintock
Barbara McClintock

... • Encode the enzyme Protein Transposase • This is required for excision (cut) and insertion (paste) • Move on their own (no intermediaries) ...
MUTATIONS TAKS QUESTIONS SPRING 2003 – 10: (22) The
MUTATIONS TAKS QUESTIONS SPRING 2003 – 10: (22) The

... (26) Ultraviolet radiation can cause mutations in the DNA of skin cells that have been overexposed to the sun. This mutated DNA has no effect on future offspring because — F changes in skin cell DNA are homozygous recessive G mutations must occur within the RNA codons H offspring reject parental ski ...
Mendelian Genetics
Mendelian Genetics

... sequence that occurs at a given locus. Alleles occur in pairs, one for each chromosome. • Dominant Alleles: an allele which masks the effect of the other allele. • Recessive Alleles: an allele which is masked by the effect of the other allele. ...
Outline Wprowadzenie do genetyki i zastosowa statystyki w
Outline Wprowadzenie do genetyki i zastosowa statystyki w

... due to time, place, reagents, personnel, … can be visible ♦ Reproducibility can easily be compromised ♦ Large amount of data, many measurements/sample (103 - 106) ♦ Highly structured/complex data (correlation, variability, etc.) ...
1 - TESTBANKcorner.EU
1 - TESTBANKcorner.EU

... recombinational analysis is that two genes that are far apart on a chromosome will have a higher frequency of recombination than two genes that are close together. Thus, if recombination between the gene of interest and a marker is very low, then the gene is likely located near that marker gene. ...
HOMEWORK #8 KEY 1. Draw a restriction map of the 20 kb DNA
HOMEWORK #8 KEY 1. Draw a restriction map of the 20 kb DNA

... sites. Either include a scale (in kb) or indicate the approximately distances between restriction sites. ...
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Genes and Variatoin

... Sources of Genetic Variation • Gene Shuffling: occurs during the production of gametes. • Independent Assortment: Chromosomes of a homologous pair moves independently during meiosis • Crossing over, also during meiosis. • Does not alter the relative frequencies of each type of allele in a populatio ...
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7.1 - DNA Structure

... 7.1.3 - State that nucleosomes help to supercoil chromosomes and help to regulate transcription During supercoiling, the DNA is condensed by a factor of x15000. The histones are responsible for the packaging of DNA at the different levels. The metaphase chromosome is an adaption for mitosis and mei ...
The Genetics of Bacteria and Their Viruses
The Genetics of Bacteria and Their Viruses

... jump from one position to another or from one DNA molecule to another • Bacteria contain a wide variety of transposable elements (as do all other organisms studied to date) • The smallest and simplest are insertion sequences, or IS elements, which are 1–3 kb in length and encode the transposase prot ...
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Chapter 01 Lecture PowerPoint

... • Genotype is the combination of alleles found in an organism • Phenotype is the visible expression of the genotype – Wild-type phenotype is the most common or generally accepted standard – Mutant alleles are usually recessive ...
Genetics of Cancer
Genetics of Cancer

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Biotechnology and Recombinant DNA
Biotechnology and Recombinant DNA

... interest from its genomic source and putting it in an expression vector. Steps: 1. Obtain the gene (PCR, restriction digest) 2. Ligate it into a vector (vector = carrier piece of DNA) 3. Transform the new recombinant DNA into bacteria/cells 4. Grow up a population of transformed cells that contain t ...
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PowerPoint slides

DNA - Ellis Benjamin
DNA - Ellis Benjamin

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Chapters 10 and 11 - Cellular Reproduction, Meiosis and Genetics
Chapters 10 and 11 - Cellular Reproduction, Meiosis and Genetics

... gene (blending of traits) are called? Incomplete dominance 8. A cross of a red cow (RR) with a white bull (WW) produces all roan offspring (RW). This type of inheritance is known as codominance 9. Variation in human skin color is a result of many genes = polygenic traits 10. If an organism’s diploid ...
Developmental Gene Expression Part I
Developmental Gene Expression Part I

... partners’ answers. ...
Created with Sketch. Genetics - true or false
Created with Sketch. Genetics - true or false

... While there are a few traits that are due to a single gene (for example, dimples and cleft chin), most traits are complex and are the result of the interactions between the protein products of several genes. All humans have almost exactly the same genes, in the same order, along our chromosomes. Our ...
Test 2 from 2012
Test 2 from 2012

... indicate the questions you want me to grade. If there is any uncertainty I will grade the first five. All students must complete Part 2 (25 pts). Part 3 will be team-based, and will be completed in class on Monday. Parts 1 and 2 together will make up 85% of your test grade, Part 3 will be worth 15%. ...
Sample Comprehensive Exam
Sample Comprehensive Exam

... C3. Creates mosaics in female mammals. C4. Cuts DNA at specific sequences. D1. Different forms of a gene. D2. Disease causing gene mutation. L. Looks for DNA damage. M. Makes copies of DNA. N. Not everyone with the genotype shows the phenotype. P1. People with the same genotype show different degree ...
Linking gene expression mentions to anatomical
Linking gene expression mentions to anatomical

... • Results range across: – 28,000 different genes (top: TNF) – 3,900 different anatomical locations (top: T cells). – 240,000 different gene/location combinations (60% mentioned once) ...
Chapter 13 Notes
Chapter 13 Notes

...  Offspring are homozygous for most traits  Pure breeds-is a selected group of organisms within a species that has been bred because of a specific characteristic they portrait Hybrids plants can increase productivity of food for humans because it is usually bigger in size and has more nutrients Gen ...
Genetics
Genetics

... Genes are a set of instructions encoded in the DNA sequence of each organism that specify the sequence of amino acids in proteins characteristic of that organism. As a basis for understanding this concept: ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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