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PH4035 - Principles of Optics
PH4035 - Principles of Optics

... understand more complicated subjects in the field of optics related to a future research project. describe the polarization properties of light with different theoretical concepts and understand most polarization phenomena understand the physics at interfaces of different refractive index; in partic ...
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... that I executed around the microscope, he might have had doubts about letting me proceed.... I was hooked because I thought the cells looked beautiful. I still do. “My original brief had been to look for changes in mitochondrial structure, with the hope of being able to relate such changes to my pre ...
Cell mechanics regulation by cytoskeleton dynamics: Combined
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... two of the priority thematic axes of the DOC2AMU program: nano-health and imaging. The supervisors are formed under an interdisciplinary perspective, as both use physical approaches to address biological questions. The biological experience is however different, Rico has a ...
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... ¡ a href=”http://www.edmundoptics.com/technical-resources-center/optics/compar of-optical-aberrations/”¿Edmunds Optical webpage¡/a¿ ¡ a href=”https://en.wikipedia.org/wiki/Optical aberration”¿WikiPedia articles aware of its weakness as first two are not.¡/a¿ ¡ a href=”https://micro.magnet.fsu.edu/pr ...
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Super-Resolution Fluorescence Microscopy by Structured Light

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... force microscopy (AFM) and scanning electron microscopy (SEM) images, and also removes all negative effects of nonuniform illumination of the sample or imperfect knowledge of the sample position as it is scanned [17]. The result is a general and extensible imaging technique that can provide a compre ...
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... have linear symmetry and are used for signal transmission over large distances, SGWs have spherical symmetry and are more useful for short-distance transmissions. Other than that, the physical principles are the same. The critical capability the SGW possesses to overcome the diffraction limit is som ...
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... NOT COMMONLY USED FOR BIOLOGICAL SYSTEMS and hence ignored B. Ray Optics Regime (D >> λ) In the ray optics regime, the size of the object is much larger than the wave lenght of the light, and a single beam can be tracked throughout the particle. (This situation is for example when whole cells are tr ...
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... Plug the laser into the upper socket on the laser bench and set up the components on the laser bench by reference to figure 2 The beam splitter is a semi transparent silvered piece of glass that both reflects and transmits light. The beam splitter needs to be set at 45 degrees to the laser and sits ...
The optical microscopy with virtual image breaks
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Young`s Double Slits
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... slide from the interference pattern of green light at a known wavelength.  Firstly, affix the green laser pen to the clamp stand, it may be useful to use a cable tie to keep the button depressed.  Place this at one end of the horizontal track and align the beam so that it is centred on the zero po ...
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Integrated optical trap holds particles for on-chip analysis
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... equal, and the particle can be moved by changing the relative power of the two laser beams. "We can also use this like an optical leaf blower to push all the particles in a sample to the same spot and increase the concentration," Schmidt said. "The goal is to control the position and movement of par ...
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Confocal microscopy



Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.
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