Image formation with broad bundles of rays
... The change in phase along different rays between points of intersection with two given wave surfaces is the same. The total change in phase between the points O and O’ is the same for the different rays. The optical path length y is the same for all these rays. ...
... The change in phase along different rays between points of intersection with two given wave surfaces is the same. The total change in phase between the points O and O’ is the same for the different rays. The optical path length y is the same for all these rays. ...
Chapter 3: Observing Microorganisms Through a Microscope
... u Special objective lenses and condenser with ring shaped diaphragm accentuate small differences in refractive indexes of internal structures. u Image: Direct rays and reflected light rays come together, forming an image with many shades of gray to black. ...
... u Special objective lenses and condenser with ring shaped diaphragm accentuate small differences in refractive indexes of internal structures. u Image: Direct rays and reflected light rays come together, forming an image with many shades of gray to black. ...
Selective plane illumination microscopy techniques in
... irradiation with short wavelengths (for example Kaede, Dendra, Dronpa and Eos). Single cells can be targeted and photo-converted from one color to another to facilitate their tracking. Photomultiplier. Glass vacuum tube in which a cascade of electrons is induced by the arrival of a single photon; fa ...
... irradiation with short wavelengths (for example Kaede, Dendra, Dronpa and Eos). Single cells can be targeted and photo-converted from one color to another to facilitate their tracking. Photomultiplier. Glass vacuum tube in which a cascade of electrons is induced by the arrival of a single photon; fa ...
Ocular Imaging for Toxicology Studies
... Ocular Imaging for Toxicology Studies: from Cornea to Retina Abstract ...
... Ocular Imaging for Toxicology Studies: from Cornea to Retina Abstract ...
Living specimen tomography by digital holographic
... RI through neuronal cells during a hypotonic stress, comparing absolute phase measurements obtained with digital holographic microscopy for two different perfusion solutions.4 However, these last two approaches allow to measure the 2D planar distribution of the RI integrated along the optical axis, ...
... RI through neuronal cells during a hypotonic stress, comparing absolute phase measurements obtained with digital holographic microscopy for two different perfusion solutions.4 However, these last two approaches allow to measure the 2D planar distribution of the RI integrated along the optical axis, ...
Lab 11 - Optical Ray Tracing
... to block off the perimetric region of the lens. Construct an optical system as follows: 1. A source with 9 rays, originating from Z=0 and slopes varying between -0.3 to 0.3. 2. An iris at Z=4.5 and Diameter D=4 3. A convex lens located from Z=5 to Z=6 with curvature of the two surfaces equal to -0.3 ...
... to block off the perimetric region of the lens. Construct an optical system as follows: 1. A source with 9 rays, originating from Z=0 and slopes varying between -0.3 to 0.3. 2. An iris at Z=4.5 and Diameter D=4 3. A convex lens located from Z=5 to Z=6 with curvature of the two surfaces equal to -0.3 ...
Correction of eye-motion artifacts in AO-OCT data sets
... measurement and complicate registration. This effect is magnified in high-resolution data sets acquired by adaptive optics (AO)-OCT instruments [1]. Recent advances in high-speed Fd-OCT acquisition [2] allow for reduction of volume acquisition time and therefore reduce eye motion artifacts; however, ...
... measurement and complicate registration. This effect is magnified in high-resolution data sets acquired by adaptive optics (AO)-OCT instruments [1]. Recent advances in high-speed Fd-OCT acquisition [2] allow for reduction of volume acquisition time and therefore reduce eye motion artifacts; however, ...
Nonconfocal Differential Interferometry Sensing Scheme for
... application of instruments for various samples to investigate the topography of specimens and the magnetic patterns. Previous efforts have confirmed that the use of a differential optical interferometer in scanning force microscope systems provides better DC stability and less optical path perturbation ...
... application of instruments for various samples to investigate the topography of specimens and the magnetic patterns. Previous efforts have confirmed that the use of a differential optical interferometer in scanning force microscope systems provides better DC stability and less optical path perturbation ...
The Pathologist`s Microscope
... The most important thing to know about your objective lenses is that they must be clean to function properly. Clean them and you will be amazed at the difference! Take them off the nosepiece and clean the end lens with a little glass cleaner on a cotton swab. In addition to magnification, objective ...
... The most important thing to know about your objective lenses is that they must be clean to function properly. Clean them and you will be amazed at the difference! Take them off the nosepiece and clean the end lens with a little glass cleaner on a cotton swab. In addition to magnification, objective ...
The principles of statistical optics and image formation A Statistical
... The principles of statistical optics and image formation A Statistical Description of Optical Science Course Objective: This course aims to introduce the intrinsic nature of optical fields, their propagation, statistical properties (i.e., coherence), and imaging methods based on statistical properti ...
... The principles of statistical optics and image formation A Statistical Description of Optical Science Course Objective: This course aims to introduce the intrinsic nature of optical fields, their propagation, statistical properties (i.e., coherence), and imaging methods based on statistical properti ...
Chapter 1: The Microbial World and You
... refraction, the light will bend as it passes from one material to another. As light passes through a glass slide, air, and the objective lens, it bends each time, causing loss of light and a blurred image. Immersion oil has the same index of refraction as glass slide, preventing light loss from ...
... refraction, the light will bend as it passes from one material to another. As light passes through a glass slide, air, and the objective lens, it bends each time, causing loss of light and a blurred image. Immersion oil has the same index of refraction as glass slide, preventing light loss from ...
Single Pixel Cameras wall panels
... of the head, but interestingly, each image is spatially identical. In other words, even though the detectors are in different positions, they do not provide different perspectives. Instead, the location of the detectors determines the apparent illumination in each image, and the perspective of the i ...
... of the head, but interestingly, each image is spatially identical. In other words, even though the detectors are in different positions, they do not provide different perspectives. Instead, the location of the detectors determines the apparent illumination in each image, and the perspective of the i ...
PROGRAM NAUCZANIA
... Before laboratories the knowledge of PhD student regarding the subject is checked. After the laboratory he/she should present a report in which the skills of obtained results analysis, as well as conclusions formulation should be demonstrated. The quality of obtained result is a measure of the skill ...
... Before laboratories the knowledge of PhD student regarding the subject is checked. After the laboratory he/she should present a report in which the skills of obtained results analysis, as well as conclusions formulation should be demonstrated. The quality of obtained result is a measure of the skill ...
Diapositive 1
... 2.2.5: Imaging with Scanning Probes (AFM, STM, SNOM). Electron Microscopy 2.2.6: Spectroscopic and MR Imaging – Biomedical applications 2.2.7: Magnetic Nanoparticles for Bioapplications 2.2.8: Fluoresence and 3D imaging visualization using confocal microscope ...
... 2.2.5: Imaging with Scanning Probes (AFM, STM, SNOM). Electron Microscopy 2.2.6: Spectroscopic and MR Imaging – Biomedical applications 2.2.7: Magnetic Nanoparticles for Bioapplications 2.2.8: Fluoresence and 3D imaging visualization using confocal microscope ...
J. Spigulis. Side-emitting optical fibers brighten our world in new
... be kept relatively consistent by keeping scattering low; then most of the launched intensity will be transmitted instead of side-emitted and, consequently, the total side-emission efficiency will be low. • Coupling light sources to both fiber ends may appear more complicated technically, but this desig ...
... be kept relatively consistent by keeping scattering low; then most of the launched intensity will be transmitted instead of side-emitted and, consequently, the total side-emission efficiency will be low. • Coupling light sources to both fiber ends may appear more complicated technically, but this desig ...
File
... Look through the eyepiece. The specimen should be visible. However, it may need to be focused. Use only the fine-wheel adjustment to sharpen the focus. CAUTION: Never use the coarse-wheel adjustment for focusing with high power. Damage to the lens and slide may result if the coarse wheel adjustment ...
... Look through the eyepiece. The specimen should be visible. However, it may need to be focused. Use only the fine-wheel adjustment to sharpen the focus. CAUTION: Never use the coarse-wheel adjustment for focusing with high power. Damage to the lens and slide may result if the coarse wheel adjustment ...
laser1
... wavelengths of the laser light are in phase in space and time. Ordinary light can be a mixture of many wavelengths. These three properties of laser light are what can make it more hazardous than ordinary light. Laser light can deposit a lot of energy within a small area. ...
... wavelengths of the laser light are in phase in space and time. Ordinary light can be a mixture of many wavelengths. These three properties of laser light are what can make it more hazardous than ordinary light. Laser light can deposit a lot of energy within a small area. ...
PHE-09 (2007
... ii) An atomic system consisting of two energy levels with population of higher energy level less than that of the lower level, is in thermal equilibrium. Show that the absorption of radiation dominates stimulated emission if radiation of appropriate frequency is introduced into the system. Comment o ...
... ii) An atomic system consisting of two energy levels with population of higher energy level less than that of the lower level, is in thermal equilibrium. Show that the absorption of radiation dominates stimulated emission if radiation of appropriate frequency is introduced into the system. Comment o ...
Total internal reflection fluorescence spectroscopy and microscopy
... Whereas confocal and multiphoton microscopy were developed during the past two decades, the first applications of Total internal reflection fluorescence microscopy (TIRFM) have been reported more than ten years earlier (Axelrod, 1981). Although its applicability is limited to cell compartments in cl ...
... Whereas confocal and multiphoton microscopy were developed during the past two decades, the first applications of Total internal reflection fluorescence microscopy (TIRFM) have been reported more than ten years earlier (Axelrod, 1981). Although its applicability is limited to cell compartments in cl ...
Machine Vision Systems as Shop Floor Metrology Tool
... seen along that particular angle, and potentially decide on which signals are the correct ones. Reflections that do not go along the view axis are not seen at all. The limitations of this approach can be more time consumed in seeking each point, and low light collection to maintain high angle separa ...
... seen along that particular angle, and potentially decide on which signals are the correct ones. Reflections that do not go along the view axis are not seen at all. The limitations of this approach can be more time consumed in seeking each point, and low light collection to maintain high angle separa ...
Towards High-Throughput Flim for Protein
... TMA sections containing 84 1.5-mm diameter tissues cores were co-stained with two fluorophore-labelled immunoglobulins: anti-2H3 labelled with Cy2 which recognises the protein ezrin and anti-T(P)250 labelled with Cy3, which recognises activated PKCD. When in close proximity, Cy2 will transfer energy ...
... TMA sections containing 84 1.5-mm diameter tissues cores were co-stained with two fluorophore-labelled immunoglobulins: anti-2H3 labelled with Cy2 which recognises the protein ezrin and anti-T(P)250 labelled with Cy3, which recognises activated PKCD. When in close proximity, Cy2 will transfer energy ...
Analysis of 258 Different Lesions of the Central Nervous System for
... CystoFlex™ UHD; Mauna Kea Technologies, Paris, France). They can be passed down the accessory channel of any standard endoscope. In this study the Gastroflex™ probe, which is 3 m long and has an internal diameter of > 2.8 mm, was used for examination. The confocal image field of view is Ø 240 µm wit ...
... CystoFlex™ UHD; Mauna Kea Technologies, Paris, France). They can be passed down the accessory channel of any standard endoscope. In this study the Gastroflex™ probe, which is 3 m long and has an internal diameter of > 2.8 mm, was used for examination. The confocal image field of view is Ø 240 µm wit ...
Photoacoustic microscopy with 2
... diode-pumped Nd: YVO4 laser 共SPOT-100-532, Elforlight兲. For optimal operation of the imaging system, the pulse repetition rate is controlled between 1 and 5 kHz. The imaging probe 共Fig. 1兲, which integrates components for delivering and focusing light, sensing photoacoustic waves, and holding the ob ...
... diode-pumped Nd: YVO4 laser 共SPOT-100-532, Elforlight兲. For optimal operation of the imaging system, the pulse repetition rate is controlled between 1 and 5 kHz. The imaging probe 共Fig. 1兲, which integrates components for delivering and focusing light, sensing photoacoustic waves, and holding the ob ...
PowerPoint 演示文稿
... image. (B) The final result of an electronic process that greatly enhances contrast and reduces "noise." (Courtesy of Bruce Schnapp.) ...
... image. (B) The final result of an electronic process that greatly enhances contrast and reduces "noise." (Courtesy of Bruce Schnapp.) ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.