PPT - Purdue University Cytometry Laboratories
... Some Key issues with regard to light • When you use a very bright light source to excite molecules in a cell, you also produce a large amount of reflected light • This light is what we measure when we measure forward and side scatter • Typically it can be 105 or 106 times the fluorescence signal pr ...
... Some Key issues with regard to light • When you use a very bright light source to excite molecules in a cell, you also produce a large amount of reflected light • This light is what we measure when we measure forward and side scatter • Typically it can be 105 or 106 times the fluorescence signal pr ...
Slide 1
... Intensity in Single-Slit Diffraction, Qualitatively To obtain the locations of the minima, the slit was equally divided into N zones, each with width Dx. Each zone acts as a source of Huygens wavelets. Now these zones can be superimposed at the screen to obtain the intensity as function of q, the a ...
... Intensity in Single-Slit Diffraction, Qualitatively To obtain the locations of the minima, the slit was equally divided into N zones, each with width Dx. Each zone acts as a source of Huygens wavelets. Now these zones can be superimposed at the screen to obtain the intensity as function of q, the a ...
Deep Horizontal Atmospheric Turbulence Modeling and Simulation with a Liquid
... characterize the LC SLM7. A linearly polarized beam at 45º enters a polarizing beam splitter (PBS1) and the reflected beam is sent to another polarizing beam splitter (PBS2) which reflects light to the BNS LC SLM which rotates the orientation of the linearly polarized beam pixel-by-pixel. At each pi ...
... characterize the LC SLM7. A linearly polarized beam at 45º enters a polarizing beam splitter (PBS1) and the reflected beam is sent to another polarizing beam splitter (PBS2) which reflects light to the BNS LC SLM which rotates the orientation of the linearly polarized beam pixel-by-pixel. At each pi ...
holography - UCSB Physics
... shutter by momentarily depressing then releasing the push-button switch. f) Turn on green clock light. As you enter the wet lab, turn right. On the counter you will see a film safe (black plastic box) containing sheets of 4”x 5” film. You will also find two optically flat glass plates. The film is s ...
... shutter by momentarily depressing then releasing the push-button switch. f) Turn on green clock light. As you enter the wet lab, turn right. On the counter you will see a film safe (black plastic box) containing sheets of 4”x 5” film. You will also find two optically flat glass plates. The film is s ...
Nondiffracting Light Beams for Long Ranges
... 5 Application of LRNB optical system to imaging We have described about LRNB generation by light beam transmission showing some examples of generated LRNBs by an optical system, up to the 3rd section. The optical system is a Galilean telescope with an eyepiece that has a spherical aberration. If we ...
... 5 Application of LRNB optical system to imaging We have described about LRNB generation by light beam transmission showing some examples of generated LRNBs by an optical system, up to the 3rd section. The optical system is a Galilean telescope with an eyepiece that has a spherical aberration. If we ...
U17301 - 3B Scientific
... Reflection of light on one edge of glass prism E11b Reflection of light on two edges of glass prism E11c Reflection of light on two glass prisms E11d Reflection of light on two glass prisms E11e Reflection of light on two glass prisms E12 Reflection of light on air prisms E13a Light ray passing a co ...
... Reflection of light on one edge of glass prism E11b Reflection of light on two edges of glass prism E11c Reflection of light on two glass prisms E11d Reflection of light on two glass prisms E11e Reflection of light on two glass prisms E12 Reflection of light on air prisms E13a Light ray passing a co ...
Image Formation by Spherical Lenses
... Spherical Lens : It is the segment of a sphere, and it refracts rays of light equally in all meridians.. Concave Lens: A concave lens causes light to spread out or diverge in which the reflecting surface curves inward. Convex Lens : A convex lens is a lens that is curved outward. The ends ...
... Spherical Lens : It is the segment of a sphere, and it refracts rays of light equally in all meridians.. Concave Lens: A concave lens causes light to spread out or diverge in which the reflecting surface curves inward. Convex Lens : A convex lens is a lens that is curved outward. The ends ...
Microsoft Office PowerPoint 2003 Beta - poster#2
... (ca 7 nm for a 20-mer), and a fluorochrome molecule. The physical dimensions of the fluorochrome we use, Alexa 532, have not been determined but are likely to be on the order of several nm in diameter (Molecular Probes, Inc.). The practical upper limit to probe length is determined by the penetratio ...
... (ca 7 nm for a 20-mer), and a fluorochrome molecule. The physical dimensions of the fluorochrome we use, Alexa 532, have not been determined but are likely to be on the order of several nm in diameter (Molecular Probes, Inc.). The practical upper limit to probe length is determined by the penetratio ...
D12 in docx
... lithography. A red laser focus exposes the photoresist, while a green laser depletes (or de-excites or “erases”) from all sides using a bottle beam. (b) Foci measured by scanning a gold bead through the focus in three dimensions. Taken from Ref.[D12:5]. ...
... lithography. A red laser focus exposes the photoresist, while a green laser depletes (or de-excites or “erases”) from all sides using a bottle beam. (b) Foci measured by scanning a gold bead through the focus in three dimensions. Taken from Ref.[D12:5]. ...
RESOLUTION OF ICCD CAMERAS
... system are in common use: the Modulation Transfer Function (MTF), the Point Spread Function (PSF), the Line Spread Function (LSF) and the Edge Spread Function (ESF)1. These methods are all linked together and rely on the characterization of the imaging system as a linear filter, which can be approxi ...
... system are in common use: the Modulation Transfer Function (MTF), the Point Spread Function (PSF), the Line Spread Function (LSF) and the Edge Spread Function (ESF)1. These methods are all linked together and rely on the characterization of the imaging system as a linear filter, which can be approxi ...
TS2
... twofold symmetric, for example by introducmode must have /k ⬍ 1, so the relevant opering capillaries with different wall thicknesses ating region in Fig. 2 is to the left of the vacuum above and below the core (Fig. 3C). Extremeline, inside one of the fingers. These conditions ly high values of bir ...
... twofold symmetric, for example by introducmode must have /k ⬍ 1, so the relevant opering capillaries with different wall thicknesses ating region in Fig. 2 is to the left of the vacuum above and below the core (Fig. 3C). Extremeline, inside one of the fingers. These conditions ly high values of bir ...
Subwavelength-diameter silica wires for low
... were studied, but their optical properties and applications remained uninvestigated8,9. It was not until a century later that researchers began to investigate the optical applications of silica wires made by drawing high-purity glass fibres from a laser-heated melt10–14. With a diameter of more than ...
... were studied, but their optical properties and applications remained uninvestigated8,9. It was not until a century later that researchers began to investigate the optical applications of silica wires made by drawing high-purity glass fibres from a laser-heated melt10–14. With a diameter of more than ...
Active imaging lens with real-time variable resolution and constant
... The proposed system has similarities with many existing ones. First, it shares some properties of a zoom system since it can increase the resolution in a given region by changing the magnification. However, it is also very different since it keeps the total field of view constant. Instead of increas ...
... The proposed system has similarities with many existing ones. First, it shares some properties of a zoom system since it can increase the resolution in a given region by changing the magnification. However, it is also very different since it keeps the total field of view constant. Instead of increas ...
10 fs ultrafast all-optical switching in polystyrene nonlinear photonic
... All-optical switching has received much attention for a long time due to its potential applications in integrated optics and ultrahigh-speed information processing. Photonic crystal all-optical switching is a promising scheme to realize switching with ultrafast response time.1–6 The refractive index ...
... All-optical switching has received much attention for a long time due to its potential applications in integrated optics and ultrahigh-speed information processing. Photonic crystal all-optical switching is a promising scheme to realize switching with ultrafast response time.1–6 The refractive index ...
Plasmonic Nearfield Scanning Probe with High Transmission
... probes will be reduced when working in air condition, because the refraction index of the optical fiber on which plasmonic lenses are fabricated is higher (usually 1.47-1.52) than air and then SPPs cannot be generated on the air side of the lens. As we have mentioned before, this problem can be solv ...
... probes will be reduced when working in air condition, because the refraction index of the optical fiber on which plasmonic lenses are fabricated is higher (usually 1.47-1.52) than air and then SPPs cannot be generated on the air side of the lens. As we have mentioned before, this problem can be solv ...
Review and Comparison of High
... The advantage of these techniques is that, besides some postprocessing for the captured raw fringe images, there is no need to make too many changes based on the original technique. Adjusting the exposure time would appear to be the best option. However, the exposure time cannot be arbitrarily chose ...
... The advantage of these techniques is that, besides some postprocessing for the captured raw fringe images, there is no need to make too many changes based on the original technique. Adjusting the exposure time would appear to be the best option. However, the exposure time cannot be arbitrarily chose ...
Student Study Guide
... and consist of a positively charged nucleus of protons and neutrons orbited by a cloud of negatively charged electrons. Heat, light, and electricity are among the things that can excite atoms. Once excited, the atom’s electrons absorb energy and briefly move from their normal orbit level to one slig ...
... and consist of a positively charged nucleus of protons and neutrons orbited by a cloud of negatively charged electrons. Heat, light, and electricity are among the things that can excite atoms. Once excited, the atom’s electrons absorb energy and briefly move from their normal orbit level to one slig ...
spatial filtering
... where “f” is the focal length of the “Fourier transform” lens. The magnification (the ratio of the size of the final image to the original) will be i/o, and you will get an image of equal size to the original when o=I=2f. Of course, you will need to have the distance between the aperture and the “F ...
... where “f” is the focal length of the “Fourier transform” lens. The magnification (the ratio of the size of the final image to the original) will be i/o, and you will get an image of equal size to the original when o=I=2f. Of course, you will need to have the distance between the aperture and the “F ...
1 L2: Reflection and Refraction c3.L2 REFLECTION AND
... Colours of objects can be explained by supposing that their surfaces reflect different proportions of the various frequency (or wavelength) components of the incident light. Different mixes of these components produce the different visual sensations that we call colour. It is worth noting in passing ...
... Colours of objects can be explained by supposing that their surfaces reflect different proportions of the various frequency (or wavelength) components of the incident light. Different mixes of these components produce the different visual sensations that we call colour. It is worth noting in passing ...
L2 REFLECTION AND REFRACTION
... Colours of objects can be explained by supposing that their surfaces reflect different proportions of the various frequency (or wavelength) components of the incident light. Different mixes of these components produce the different visual sensations that we call colour. It is worth noting in passing ...
... Colours of objects can be explained by supposing that their surfaces reflect different proportions of the various frequency (or wavelength) components of the incident light. Different mixes of these components produce the different visual sensations that we call colour. It is worth noting in passing ...
Nanostructured Holograms for Broadband Manipulation of Vector
... was captured by a CCD camera. By rotating the linear polarizer in front of the beam with the characteristic doughnut intensity profile (m = +1 diffraction order), we demonstrated that its polarization is oriented radially, in agreement with Figure 2. As expected from the design, radially polarized bea ...
... was captured by a CCD camera. By rotating the linear polarizer in front of the beam with the characteristic doughnut intensity profile (m = +1 diffraction order), we demonstrated that its polarization is oriented radially, in agreement with Figure 2. As expected from the design, radially polarized bea ...
6.1 Characteristics Because VCSELs emit from the top surface of the
... to the interaction between the laser frequency chirp and fiber dispersion. This is not an issue for enterprise networks which are short distance, but could be a serious limiting factor for telecommunications applications. To overcome this limitation, the laser diode is operated in a CW mode, and out ...
... to the interaction between the laser frequency chirp and fiber dispersion. This is not an issue for enterprise networks which are short distance, but could be a serious limiting factor for telecommunications applications. To overcome this limitation, the laser diode is operated in a CW mode, and out ...
6.0 Mb - Todd Satogata
... Polarization and the Brewster Angle § When the angle of incidence is equal to the polarizing angle, n2 tan ✓P = n1 also called the Brewster angle, the reflected beam has no polarization in the plane defined by the incident and reflected rays. – The polarizing angle occurs when the reflected and ...
... Polarization and the Brewster Angle § When the angle of incidence is equal to the polarizing angle, n2 tan ✓P = n1 also called the Brewster angle, the reflected beam has no polarization in the plane defined by the incident and reflected rays. – The polarizing angle occurs when the reflected and ...
Microscopy
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy.Optical and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning of a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object of interest. The development of microscopy revolutionized biology and remains an essential technique in the life and physical sciences.