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Past iGEM Projects: Case Studies
Past iGEM Projects: Case Studies

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this resource 4.28 MB

... • I have reviewed this material in accordance with U.S. Copyright Law and have tried to maximize your ability to use, share, and adapt it. The citation key on the following slide provides information about how you may share and adapt this material. • Copyright holders of content included in this mat ...
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... substrates. We will use NorM from Erwinia amylovora as model transporter. In our previous studies, we could show that NorM is involved in the resistance of E. amylovora towards antibiotics produced by other bacteria found on plants. NorM belongs to the multidrug and toxin extrusion (MATE) family of ...
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Horizontal gene transfer



Horizontal gene transfer (HGT) refers to the transfer of genes between organisms in a manner other than traditional reproduction. Also termed lateral gene transfer (LGT), it contrasts with vertical transfer, the transmission of genes from the parental generation to offspring via sexual or asexual reproduction. HGT has been shown to be an important factor in the evolution of many organisms.Horizontal gene transfer is the primary reason for bacterial antibiotic resistance, and plays an important role in the evolution of bacteria that can degrade novel compounds such as human-created pesticides and in the evolution, maintenance, and transmission of virulence. This horizontal gene transfer often involves temperate bacteriophages and plasmids. Genes that are responsible for antibiotic resistance in one species of bacteria can be transferred to another species of bacteria through various mechanisms (e.g., via F-pilus), subsequently arming the antibiotic resistant genes' recipient against antibiotics, which is becoming a medical challenge to deal with.Most thinking in genetics has focused upon vertical transfer, but there is a growing awareness that horizontal gene transfer is a highly significant phenomenon and among single-celled organisms perhaps the dominant form of genetic transfer.Artificial horizontal gene transfer is a form of genetic engineering.
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