Requirements for CPC localization during anaphase
... Bir1p (Survivin homolog) in yeast, are found in ...
... Bir1p (Survivin homolog) in yeast, are found in ...
Cellular function and pathological role of ATP13A2 and related P
... P-type ATPases are biological pumps omnipresent in all forms of life, which are recognized by several conserved signature motifs associated with their catalytic mechanism (Axelsen and Palmgren, 1998). The main characteristic of all P-type ATPases is the formation of an acid-stable aspartyl phosphate ...
... P-type ATPases are biological pumps omnipresent in all forms of life, which are recognized by several conserved signature motifs associated with their catalytic mechanism (Axelsen and Palmgren, 1998). The main characteristic of all P-type ATPases is the formation of an acid-stable aspartyl phosphate ...
The Multiple Personalities of the Regulatory Subunit of Protein
... role in the specific degradation of cyclin B at the end of mitosis [54, 55]. This motif, which was first recognized in CK2β by Allende and Allende, contains three highly conserved residues conforming to the general destruction box consensus RXXLXXXXN/D[38, 55]. Interestingly, this motif is located o ...
... role in the specific degradation of cyclin B at the end of mitosis [54, 55]. This motif, which was first recognized in CK2β by Allende and Allende, contains three highly conserved residues conforming to the general destruction box consensus RXXLXXXXN/D[38, 55]. Interestingly, this motif is located o ...
as Hotspot Ca 2+ Signaling Units
... evidence has been provided that the Na+/Ca2+ exchanger isoform NCLX is the longsought protein responsible for the mitochondrial Na+-dependent Ca2+ efflux [33]. Finally, the low conductance mode of the permeability transition pore (PTP), a channel of still debated nature localized in the IMM [34], ca ...
... evidence has been provided that the Na+/Ca2+ exchanger isoform NCLX is the longsought protein responsible for the mitochondrial Na+-dependent Ca2+ efflux [33]. Finally, the low conductance mode of the permeability transition pore (PTP), a channel of still debated nature localized in the IMM [34], ca ...
Mechanisms for the Carcinostatic Activity of Se
... mutations of deoxyribonucleic acid (DNA) that may lead to cancer. Selenite, selenomethionine, 1,4phenylene-bis(methylene)selenocyanate, (p-XSC), other aliphatic selenocyanates, (RSeCN), selenobetaine, Semethylselenocysteine (SeMC) as well as other selenium compounds have been shown to inhibit the in ...
... mutations of deoxyribonucleic acid (DNA) that may lead to cancer. Selenite, selenomethionine, 1,4phenylene-bis(methylene)selenocyanate, (p-XSC), other aliphatic selenocyanates, (RSeCN), selenobetaine, Semethylselenocysteine (SeMC) as well as other selenium compounds have been shown to inhibit the in ...
Functional Characterisation of Plant
... only dually located protein transporter. Furthermore this protein is predicted to contain a conserved PRAT domain and sterile alpha motif (SAM) domain. The latter of which is known to be involved in protein-protein interaction and RNA binding. Utilising T-DNA insertional knockout lines, the function ...
... only dually located protein transporter. Furthermore this protein is predicted to contain a conserved PRAT domain and sterile alpha motif (SAM) domain. The latter of which is known to be involved in protein-protein interaction and RNA binding. Utilising T-DNA insertional knockout lines, the function ...
Cytoskeleton: What Does GTP Do for Septins? Dispatch
... GTP hydrolysis have been key tools for elucidating cellular function, and the same will likely be true for septins. Septin sequences diverge from Ras at key residues implicated in catalyzing GTP hydrolysis, and so far, GTPase-deficient mutants have not been reported. Expression of septins with mutat ...
... GTP hydrolysis have been key tools for elucidating cellular function, and the same will likely be true for septins. Septin sequences diverge from Ras at key residues implicated in catalyzing GTP hydrolysis, and so far, GTPase-deficient mutants have not been reported. Expression of septins with mutat ...
BMP binding domains in the extracellular space
... The full-length Xenopus procollagen IIA cDNA was excised with EcoRI from a pUC18 vector (a kind gift of Dr Francesco Ramirez) and ligated to pCS2. For the type IIA procollagen CR construct a PCR fragment (440 bp) containing the CR domain in a SacI-XbaI fragment was cloned into the same pCS2 expressi ...
... The full-length Xenopus procollagen IIA cDNA was excised with EcoRI from a pUC18 vector (a kind gift of Dr Francesco Ramirez) and ligated to pCS2. For the type IIA procollagen CR construct a PCR fragment (440 bp) containing the CR domain in a SacI-XbaI fragment was cloned into the same pCS2 expressi ...
Replication of damaged DNA in vitro is blocked
... between p53 and the DNA replication machinery is provided by the observations that p53 is localized to foci of replication in the nuclei of herpes virus-infected cells (34) and that p53 is imported into the nucleus of Xenopus oocyte upon fertilization, although the ®rst 12 rapid cell cycles occur wi ...
... between p53 and the DNA replication machinery is provided by the observations that p53 is localized to foci of replication in the nuclei of herpes virus-infected cells (34) and that p53 is imported into the nucleus of Xenopus oocyte upon fertilization, although the ®rst 12 rapid cell cycles occur wi ...
Stepwise Activation of BAX and BAK by tBID, BIM, and PUMA
... apoptosis. The permeabilization of the MOM not only couples the mitochondria to the activation of caspases but also initiates caspase-independent mitochondrial dysfunction (Cheng et al., 2001). The BCL-2 family proteins control a crucial checkpoint of apoptosis at the mitochondria (Cory and Adams, 2 ...
... apoptosis. The permeabilization of the MOM not only couples the mitochondria to the activation of caspases but also initiates caspase-independent mitochondrial dysfunction (Cheng et al., 2001). The BCL-2 family proteins control a crucial checkpoint of apoptosis at the mitochondria (Cory and Adams, 2 ...
β-catenin: a key mediator of Wnt signaling Karl
... (Figure 2a). Several somewhat unrelated proteins also contain arm repeats, including the product of the tumor suppressor adenomatous polyposis coli (APC), the src substrate p120, and the nuclear import factor importin [18]. The amino terminus of β-catenin is known to be important for regulating the ...
... (Figure 2a). Several somewhat unrelated proteins also contain arm repeats, including the product of the tumor suppressor adenomatous polyposis coli (APC), the src substrate p120, and the nuclear import factor importin [18]. The amino terminus of β-catenin is known to be important for regulating the ...
Presequence-Independent Manner Transported by the TIM23
... Complementation of the yeast SYM1 deletion by expression of the human MPV17 gene defines these two proteins as functional orthologs and has established Sym1 as a model for MPV17 function (63). The observed reduction of steady-state protein levels of mutated MPV17 in patient cells has raised question ...
... Complementation of the yeast SYM1 deletion by expression of the human MPV17 gene defines these two proteins as functional orthologs and has established Sym1 as a model for MPV17 function (63). The observed reduction of steady-state protein levels of mutated MPV17 in patient cells has raised question ...
Increased and controlled expression of the Rickettsia
... bond reducing agents (but not thiol reagents), suggesting the presence of an intramolecular disulfide bond in the ATP/ ADP translocase that is essential for transport activity (Plano & Winkler, 1991). These data were used to propose an initial topological model of the R. prowaxekii ATP/ADP transloca ...
... bond reducing agents (but not thiol reagents), suggesting the presence of an intramolecular disulfide bond in the ATP/ ADP translocase that is essential for transport activity (Plano & Winkler, 1991). These data were used to propose an initial topological model of the R. prowaxekii ATP/ADP transloca ...
Protein kinase C- modulates mitochondrial function and active Na
... is a critical step in the phosphorylation of target proteins and PKC-⑀ signaling. The mitochondrion is thought to be an important target for PKC-⑀ during cardiac ischemia-reperfusion injury. Ischemic preconditioning or phorbol ester induces the translocation of PKC-⑀ to the mitochondria followed by ...
... is a critical step in the phosphorylation of target proteins and PKC-⑀ signaling. The mitochondrion is thought to be an important target for PKC-⑀ during cardiac ischemia-reperfusion injury. Ischemic preconditioning or phorbol ester induces the translocation of PKC-⑀ to the mitochondria followed by ...
Direct Interaction between Survivin and Smac/DIABLO Is Essential
... of cytochrome c into the cytosol from the mitochondrial intermembrane space (33). To further investigate whether the mitochondria are involved in this event, Taxol-treated HeLa cells were first lysed and divided into membrane pellet and cytosolic fraction. The pellet was then treated with 0.5% bile ...
... of cytochrome c into the cytosol from the mitochondrial intermembrane space (33). To further investigate whether the mitochondria are involved in this event, Taxol-treated HeLa cells were first lysed and divided into membrane pellet and cytosolic fraction. The pellet was then treated with 0.5% bile ...
Protein stabilization: a common consequence of mutations
... Myc is overexpressed in many cancers as a result of gene rearrangement or ampli®cation, but coding sequence changes which cluster in the N-terminal transactivation domain also appear to play a role in tumour progression. The prototypic v-Myc gene of MC29 virus diers from avian c-Myc by a series of ...
... Myc is overexpressed in many cancers as a result of gene rearrangement or ampli®cation, but coding sequence changes which cluster in the N-terminal transactivation domain also appear to play a role in tumour progression. The prototypic v-Myc gene of MC29 virus diers from avian c-Myc by a series of ...
Human Cardiac Troponin Complex. Structure and Functions
... on artificial troponin complex composed of different animal troponins, by means of site-directed spin labeling (SDSL-EPR) it was shown that the region limited by residues 138-145 does not possess a stable secondary structure. In the work by Takeda et al. [67], they were not able to crystallize the i ...
... on artificial troponin complex composed of different animal troponins, by means of site-directed spin labeling (SDSL-EPR) it was shown that the region limited by residues 138-145 does not possess a stable secondary structure. In the work by Takeda et al. [67], they were not able to crystallize the i ...
Chicken Acidic Leucine-rich EGF-like Domain Containing Brain
... CALEB-encoding cDNAs were obtained by screening an adult chicken eye cDNA library constructed in lgt11 with mAb 4/1. Additional overlapping clones were found in the same library using the 1-kb insert of the initial cDNA clone as a probe. The amino acid sequence deduced from these overlapping cDNA cl ...
... CALEB-encoding cDNAs were obtained by screening an adult chicken eye cDNA library constructed in lgt11 with mAb 4/1. Additional overlapping clones were found in the same library using the 1-kb insert of the initial cDNA clone as a probe. The amino acid sequence deduced from these overlapping cDNA cl ...
Molecular Intercommunication between the Complement and
... remain unknown. The current study reports multiple links for various factors of the coagulation and fibrinolysis cascades with the central complement components C3 and C5 in vitro and ex vivo. Thrombin, human coagulation factors (F) XIa, Xa, and IXa, and plasmin were all found to effectively cleave ...
... remain unknown. The current study reports multiple links for various factors of the coagulation and fibrinolysis cascades with the central complement components C3 and C5 in vitro and ex vivo. Thrombin, human coagulation factors (F) XIa, Xa, and IXa, and plasmin were all found to effectively cleave ...
The role of c-di-GMP signaling in an Aeromonas veronii biovar
... niches (hospital sewage water, lagoon where duckweed is grown for sewage water purification, fish culture pond where sewage-grown duckweed was used as fish feed and control environmental pond). MT-G was not only detected 2007 Federation of European Microbiological Societies Published by Blackwell Pu ...
... niches (hospital sewage water, lagoon where duckweed is grown for sewage water purification, fish culture pond where sewage-grown duckweed was used as fish feed and control environmental pond). MT-G was not only detected 2007 Federation of European Microbiological Societies Published by Blackwell Pu ...
Supplementary Data - Institute of Cancer Research
... necessary and sufficient to resolve IMD activation in the presence of PGN (see also Supplementary Fig. 3c). After TCT monomer injections, the heterodimeric LCx/LCa module was required to relay signal, in agreement with previous work. However, no significant increase was observed when either rLCx or ...
... necessary and sufficient to resolve IMD activation in the presence of PGN (see also Supplementary Fig. 3c). After TCT monomer injections, the heterodimeric LCx/LCa module was required to relay signal, in agreement with previous work. However, no significant increase was observed when either rLCx or ...
Cell regulation by the Apc protein Apc as master regulator of epithelia
... The first recognised function of Apc was its role in Wnt signalling [4,5]. This function is one of the driving forces for how mutations in Apc ensure that cells remain proliferative. Many of the molecular details of this pathway have been described extensively in many reviews [6]. Apc negatively ...
... The first recognised function of Apc was its role in Wnt signalling [4,5]. This function is one of the driving forces for how mutations in Apc ensure that cells remain proliferative. Many of the molecular details of this pathway have been described extensively in many reviews [6]. Apc negatively ...
The EMBO Journal (2002) 21, 1704–1712
... the MYST family of HATs, a family that is conserved from yeast to mammals (Sterner and Berger, 2000). Members of this family are characterized by a conserved catalytic domain, the MYST domain. In addition to the MYST domain, Tip60 contains a chromodomain at its N-terminus. Chromodomains are found in ...
... the MYST family of HATs, a family that is conserved from yeast to mammals (Sterner and Berger, 2000). Members of this family are characterized by a conserved catalytic domain, the MYST domain. In addition to the MYST domain, Tip60 contains a chromodomain at its N-terminus. Chromodomains are found in ...
Meiotic and Mitotic Recombination in Meiosis
... a four-stranded intermediate now known as the Holliday junction. Holliday proposed that these junctions are cleaved by DNA repair enzymes, now known as resolvases, to reestablish two separate duplexes. Depending on which strands are nicked, this process, now called resolution, could result in crosso ...
... a four-stranded intermediate now known as the Holliday junction. Holliday proposed that these junctions are cleaved by DNA repair enzymes, now known as resolvases, to reestablish two separate duplexes. Depending on which strands are nicked, this process, now called resolution, could result in crosso ...
Apoptosome
The apoptosome is a large quaternary protein structure formed in the process of apoptosis. Its formation is triggered by the release of cytochrome c from the mitochondria in response to an internal (intrinsic) or external (extrinsic) cell death stimulus. Stimuli can vary from DNA damage and viral infection to developmental cues such as those leading to the degradation of a tadpole's tail.In mammalian cells, once cytochrome c is released, it binds to the cytosolic protein Apaf-1 to facilitate the formation of apoptosome. An early biochemical study suggests a two-to-one ratio of cytochrome c to apaf-1 for apoptosome formation. However, recent structural studies suggest the cytochrome c to apaf-1 ratio is one-to-one. It has also been shown that the nucleotide dATP as third component binds to apaf-1, however its exact role is still debated. The mammalian apoptosome had never been crystallized, but a human APAF-1/cytochrome-c apoptosome has been imaged at lower (2 nm) resolution by cryogenic transmission electron microscopy 10 years ago, revealing a wheel-like particle with 7-fold symmetry. Recently, a medium resolution (9.5 Ångström) structure of human apoptosome was also solved by cryo-electron microscopy, which allows unambiguous inference for positions of all the APAF-1 domains (CARD, NBARC and WD40) and cytochrome c. There is also now a crystal structure of the monomeric, inactive Apaf-1 subunit (PDB 3SFZ). Once formed, the apoptosome can then recruit and activate the inactive pro-caspase-9. Once activated, this initiator caspase can then activate effector caspases and trigger a cascade of events leading to apoptosis.