siRNA expression vector pRNAT-H1
... This vector with attB1 and attB2 sequences flanking the gene of interest is compatible with Invitrogen Gateway Technology and can be used to move DNA sequence (any genes) into multiple vector systems for ...
... This vector with attB1 and attB2 sequences flanking the gene of interest is compatible with Invitrogen Gateway Technology and can be used to move DNA sequence (any genes) into multiple vector systems for ...
Chapter 20.
... Restriction sites for splicing in gene of interest Selectable marker Plasmid has both “added” gene & antibiotic resistance gene If bacteria don’t pick up plasmid then die on antibiotic plates If bacteria pick up plasmid then survive on antibiotic plates selecting for successful AP Biology ...
... Restriction sites for splicing in gene of interest Selectable marker Plasmid has both “added” gene & antibiotic resistance gene If bacteria don’t pick up plasmid then die on antibiotic plates If bacteria pick up plasmid then survive on antibiotic plates selecting for successful AP Biology ...
Gene regulation - Napa Valley College
... repressor protein, which turns the operon off The repressor is active only in the presence of its corepressor tryptophan; thus the trp operon is turned off (repressed) if tryptophan levels are ...
... repressor protein, which turns the operon off The repressor is active only in the presence of its corepressor tryptophan; thus the trp operon is turned off (repressed) if tryptophan levels are ...
AP Biology: Unit 3B Homework
... 4. How does the lac operon regulate the digestion of lactose in bacteria? 5. Describe how the lac operon is an inducible operon. 6. How does the presence and absence of glucose influence the lac operon? 7. Identify four locations where regulation of gene expression in eukaryotic cells is possible. 8 ...
... 4. How does the lac operon regulate the digestion of lactose in bacteria? 5. Describe how the lac operon is an inducible operon. 6. How does the presence and absence of glucose influence the lac operon? 7. Identify four locations where regulation of gene expression in eukaryotic cells is possible. 8 ...
Control of Gene Expression
... galactosidase if there is lactose in the environment to digest There is no point in making the enzyme if there is no lactose sugar to break down It is the combination of the promoter and the DNA that regulate when a gene will be transcribed ...
... galactosidase if there is lactose in the environment to digest There is no point in making the enzyme if there is no lactose sugar to break down It is the combination of the promoter and the DNA that regulate when a gene will be transcribed ...
Inquiry into Life Twelfth Edition
... •The AraC, ara control protein, acts as both a positive and negative regulator •There are 3 binding sites • Far upstream site, araO2 • araO1 located between -106 and -144 • araI is really 2 half-sites – araI1 between -56 and -78 – araI2 -35 to -51 – Each half-site can bind one monomer of AraC ...
... •The AraC, ara control protein, acts as both a positive and negative regulator •There are 3 binding sites • Far upstream site, araO2 • araO1 located between -106 and -144 • araI is really 2 half-sites – araI1 between -56 and -78 – araI2 -35 to -51 – Each half-site can bind one monomer of AraC ...
Overview of Carbohydrate Digestion and Metabolism
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
Overview of Carbohydrate Digestion and Metabolism
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
Overview of Carbohydrate Digestion and Metabolism
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
Overview of Carbohydrate Digestion and Metabolism
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
... Glucose acetyl CoA Glucose to glycerol for triglyceride synthesis Liver releases glucose for other tissues ...
Molecules of Life
... What is the structure of nucleic acids? They are polymers of nucleotides What do the nucleotides contain? ...
... What is the structure of nucleic acids? They are polymers of nucleotides What do the nucleotides contain? ...
The methanol oxidation genes mxaFJGIR(S)ACKLD in
... third (non-essential) subunit of MDH [6] or a molecular chaperone [5]. The genes mxaFJGI are transcribed from a promoter upstream of mxaF ; this is the only promoter so far de¢nitively identi¢ed in a methylotroph [4,7]. About 2 kb downstream from mxaI in Methylobacterium extorquens is another cluste ...
... third (non-essential) subunit of MDH [6] or a molecular chaperone [5]. The genes mxaFJGI are transcribed from a promoter upstream of mxaF ; this is the only promoter so far de¢nitively identi¢ed in a methylotroph [4,7]. About 2 kb downstream from mxaI in Methylobacterium extorquens is another cluste ...
The Co-Evolution of Genes and Culture Pedigrees and the
... disaccharide. Infant mammals produce the enzyme lactase in their small intestines. Lactase breaks down lactose into glucose and galactose. These two simpler sugars, or monosaccharides, are easily absorbed through the small intestine and into the bloodstream. The blood delivers the sugars throughout ...
... disaccharide. Infant mammals produce the enzyme lactase in their small intestines. Lactase breaks down lactose into glucose and galactose. These two simpler sugars, or monosaccharides, are easily absorbed through the small intestine and into the bloodstream. The blood delivers the sugars throughout ...
- City Research Online
... lactase. Such conditions include Crohns disease, gastroenteritis and surgery, and it can also occur as a complication of anti cancer drugs. In these cases the lactose deficiency may be temporary, but it can also be permanent. Congenital lactase deficiency is present from birth. It is extremely rare; ...
... lactase. Such conditions include Crohns disease, gastroenteritis and surgery, and it can also occur as a complication of anti cancer drugs. In these cases the lactose deficiency may be temporary, but it can also be permanent. Congenital lactase deficiency is present from birth. It is extremely rare; ...
chapter 3 - Fullfrontalanatomy.com
... 7. Many analogies relate to students the diversity of proteins that can be made from just 20 amino acids. The authors note that our language uses combinations of 26 letters to form words. Proteins are much longer “words,” creating even more diversity. Another analogy is to trains. This builds on the ...
... 7. Many analogies relate to students the diversity of proteins that can be made from just 20 amino acids. The authors note that our language uses combinations of 26 letters to form words. Proteins are much longer “words,” creating even more diversity. Another analogy is to trains. This builds on the ...
Oxidative Metabolism - Plant Energy Biology
... Control of mitochondrial gene expression Nuclear encoded mitochondrial gene expression ...
... Control of mitochondrial gene expression Nuclear encoded mitochondrial gene expression ...
Project : Operon Prediction - Bioinformatics at School of Informatics
... The biological functions of some genes are still unknown. There is only a few promoter detection algorithms, but they are not fully satisfactory. In many cases, genomic data files do not provide full information of genes and their products. ( e.g. gene name, COG, PID.) Operon tends to undergo multip ...
... The biological functions of some genes are still unknown. There is only a few promoter detection algorithms, but they are not fully satisfactory. In many cases, genomic data files do not provide full information of genes and their products. ( e.g. gene name, COG, PID.) Operon tends to undergo multip ...
DNA structure and protein synthesis
... Protein Processing and Degradation • After translation, various types of protein processing, including cleavage and the addition of chemical groups, are subject to control • Proteasomes are giant protein complexes that bind protein molecules and degrade them ...
... Protein Processing and Degradation • After translation, various types of protein processing, including cleavage and the addition of chemical groups, are subject to control • Proteasomes are giant protein complexes that bind protein molecules and degrade them ...
Molecular Genetics - Ursuline High School
... particular protein. The first operon discovered is known as the “lac-operon”. ...
... particular protein. The first operon discovered is known as the “lac-operon”. ...
Slide ()
... Transport of glucose, fructose, and galactose across the intestinal epithelium. The SGLT1 transporter is coupled to the Na+-K+ pump, allowing glucose and galactose to be transported against their concentration gradients. The GLUT5 Na+-independent facilitative transporter allows fructose, as well as ...
... Transport of glucose, fructose, and galactose across the intestinal epithelium. The SGLT1 transporter is coupled to the Na+-K+ pump, allowing glucose and galactose to be transported against their concentration gradients. The GLUT5 Na+-independent facilitative transporter allows fructose, as well as ...
methods - Nature
... the ratios of interactor to target necessary for a complete structural titration. This method can be combined with in vitro methodology, for example, by exogenously adding a small interacting molecule to cell cultures expressing an endogenously labeled protein. The most comprehensive experimental pr ...
... the ratios of interactor to target necessary for a complete structural titration. This method can be combined with in vitro methodology, for example, by exogenously adding a small interacting molecule to cell cultures expressing an endogenously labeled protein. The most comprehensive experimental pr ...
Study of the arginine repressor in different organisms
... The arginine repressor (ArgR) regulates transcription of the arginine biosynthetic genes in bacteria. ArgR proteins play a multifunctional role in the bacterial cell. They inhibit biosynthetic promoters and are involved in activation of several catabolic pathways. The arginine repressor of Streptomy ...
... The arginine repressor (ArgR) regulates transcription of the arginine biosynthetic genes in bacteria. ArgR proteins play a multifunctional role in the bacterial cell. They inhibit biosynthetic promoters and are involved in activation of several catabolic pathways. The arginine repressor of Streptomy ...
11.7 Repressor binds cooperatively at each operator using a helix
... phage of the same type from infecting a cell. It results from the synthesis of phage repressor by the prophage genome. Induction refers to the ability of bacteria (or yeast) to synthesize certain enzymes only when their substrates are present; applied to gene expression, refers to switching on trans ...
... phage of the same type from infecting a cell. It results from the synthesis of phage repressor by the prophage genome. Induction refers to the ability of bacteria (or yeast) to synthesize certain enzymes only when their substrates are present; applied to gene expression, refers to switching on trans ...
Lac operon
lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes at universities for this reason.Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. LacY encodes lactose permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes galactoside O-acetyltransferase. Layout of the lac operon.It would be wasteful to produce the enzymes when there is no lactose available or if there is a more preferable energy source available, such as glucose. The lac operon uses a two-part control mechanism to ensure that the cell expends energy producing the enzymes encoded by the lac operon only when necessary. In the absence of lactose, the lac repressor halts production of the enzymes encoded by the lac operon. In the presence of glucose, the catabolite activator protein (CAP), required for production of the enzymes, remains inactive, and EIIAGlc shuts down lactose permease to prevent transport of lactose into the cell. This dual control mechanism causes the sequential utilization of glucose and lactose in two distinct growth phases, known as diauxie.