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... To create enough DNA from a small sample to create a DNA profile. ...
Introduction to DNA Computing
Introduction to DNA Computing

... Recombinant DNA Technology •Cleavage DNA at specific sites by restriction enzymes,which greatly facilitates the isolation and manipulation of individual DNA. •Rapid sequencing of all the nucleotides in a purified DNA fragment, which makes it possible to determine the boundaries of a gene and the am ...
No Slide Title
No Slide Title

... Early on - “Template” so molecules can line up in a specific order and be joined to create a new macromolecule 1940s - DNA = genetic material 1950s - structure identified how it could act as a template for replication and transmission of genetic info One strand is the complement of the other ...
DNA metabolism
DNA metabolism

... Early on - “Template” so molecules can line up in a specific order and be joined to create a new macromolecule 1940s - DNA = genetic material 1950s - structure identified how it could act as a template for replication and transmission of genetic info One strand is the complement of the other ...
Nucleic Acids
Nucleic Acids

... 3. Inheritance is based on replication of the DNA double helix • An RNA molecule is single polynucleotide chain. • DNA molecules have two polynucleotide strands that spiral around an imaginary axis to form a double helix. • The double helix was first proposed as the structure of DNA in 1953 by Jame ...
Poster
Poster

... which also makes it one of the largest molecules. Its function is to surround the DNA, unwind it, separate it into two strands, and use the DNA template strand to create a messenger RNA (mRNA) copy of a gene. These mRNA copies of genes are needed by the cell to make proteins to keep the cell healthy ...
PCR
PCR

... PCR (Polymerase Chain Reaction) ● PCR ○ 1983 first discovered by Kary Mullis ○ used to amplify a single copy or a few copies of DNA in order to create 1000s or millions of copies of a particular DNA sequence ○ OVERVIEW ■ relies on thermal cycling, consisting of repeated heated and cooling reactions ...
A Biology Primer for Computer Scientists
A Biology Primer for Computer Scientists

... DNA replication is the process by which a double-stranded DNA sequence produces two double-stranded sequences identical (in the absence of errors!) to the original one. The way this happens is that the original complementary strands unwind and for each of them a new complementary strand is synthesiz ...
Nylon/DNA: Single-Stranded DNA with a Covalently Stitched Nylon
Nylon/DNA: Single-Stranded DNA with a Covalently Stitched Nylon

... have expanded from genetic information carriers and messengers to include catalysis and regulation of a number of cellular processes.1 In addition, many nucleic acid-based structures have been developed with medicinal applications, catalytic properties, and prebiotic chemistry implications.1 Notable ...
11.2 Meiosis
11.2 Meiosis

... 2. Chromosomes do not separate at centromeres D. Telophase I 1. The two new cells contain half the number of chromosomes 2. Called reduction division III. Meiosis II A. Identical to stages of mitosis with a few exceptions 1. Chromosomes do not replicate before they divide 2. In the end 4 haploid cel ...
Genes: Structure, Replication, & Mutation
Genes: Structure, Replication, & Mutation

... Flat, planar, aromatic molecules that can fit between the stacked bases (intercalate) in the center of a DNA double helix and distort its geometry The distortion somehow induces single nucleotide insertions or deletions ...
Chapter 24: Genes and Chromosomes
Chapter 24: Genes and Chromosomes

... Ans: Introns are regions of genes (primarily eukaryotic) that in mRNA are transcribed but are not translated. They do not code for amino acid sequences within the protein that is coded by the gene. Thus they interrupt the colinearity between the nucleotide sequence of the gene and the amino acid seq ...
TRANSPOSABLE ELEMENTS IN BACTERIA Transposable
TRANSPOSABLE ELEMENTS IN BACTERIA Transposable

... The multiplicity of transpositional and recombinational events associated with TEs allows them to unlock the Pandora's box of genome plasticity for bacterial chromosomes and plasmids in which they are found. The K-12 laboratory strains of E. coli show considerable variability in the number and locat ...
Replication
Replication

... being truncated, the chromosomal DNAs carry special buffer regions at their termini, called telomeres. Telomeres are repeats, many thousand times, of a very simple motif. For all chromosomes in all humans (actually, in all vertebrates) the repeating sequence is: 5’TTAGGG3’. Mostly it is dsDNA but at ...
DNA
DNA

... open strands to form two new strands of DNA, aided by enzymes called DNA polymerese. DNA polymerese: an enzyme that helps new nucleotides bond onto open strand of DNA during replication. ...
Name
Name

... Estimate the length of the DNA strands in your sample. Write the three numbers below, starting with the longest strand. ...
Lecture 7 DNA REPLICATION
Lecture 7 DNA REPLICATION

... EM picture of two topoisomers (molecules differ in linking numbers) showing relaxed circular and negatively supercoiled DNA ...
AP Biology, Chapter 16 The Molecular Basis of Inheritance Life`s
AP Biology, Chapter 16 The Molecular Basis of Inheritance Life`s

... Strands run in opposite directions; 3'5' and 5'3' AT and GC pairs give a constant 2 nm width Also satisfies Chargaff's rule (equilvalencies) 6. Describe the structure of DNA. Explain the "base-pairing rule" and describe its significance. Double stranded helix Sugar-phosphate backbones AT and GC pa ...
DNA Replication
DNA Replication

... ■ The replication of DNA is semi-conservative and depends on complementary base pairing. ...
DNA Replication - Der Lernberater
DNA Replication - Der Lernberater

... Initiates the formation of bidirectional replication forks ...
Welcome to the Chapter 12 Test!
Welcome to the Chapter 12 Test!

... 8. Explain why DNA replication is considered a chemical reaction, and explain why this reaction will not occur without the help of enzymes. ...
MB207Jan2010
MB207Jan2010

... order for chiasmata to form where crossing-over occurs – DNA synapsis: base pairing between complementary strands from 2 DNA molecules – Chiasmata: regions where paired homologous chromosomes exchange genetic material during meiosis, a cross-shaped structure • Only occurs between homologous DNA mole ...
DNA replication
DNA replication

... • Cos sequences are single stranded sequences of DNA, which have been split from the parent molecule by a specific restriction enzyme in such a way that the ends have specific affinity for each other, and hence are known as cohesive ends. • Cosmids are packaged in phage structures consisting of prot ...
Replication 1
Replication 1

... 1. At a nick (free 3’ OH) in the DNA the DNA pol I binds and digests nucleotides in a 5’-3’ direction 2. The DNA polymerase activity synthesizes a new DNA strand 3. A nick remains as the DNA pol I dissociates from the ds DNA. 4. The nick is closed via DNA ligase ...
to Unit 10 Notes
to Unit 10 Notes

... Introns – sequences in the DNA that are NOT used to make mRNA or to make a protein. They are NOT transcribed *  Exons – sequences in the DNA that are expressed or used to make mRNA and and ultimately are used to make a protein ...
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Holliday junction



A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.
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