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Ammonia, urea production and pH regulation
Ammonia, urea production and pH regulation

... control is transcriptionally effected by changes in enzyme concentrations. Substrate provision for the urea cycle depends on amino acid delivery, the activity of amino acid transport systems, and amino acid metabolizing enzymes, and these factors are therefore important determinants of urea synthesi ...
Metabolic processes of Methanococcus maripaludis and potential
Metabolic processes of Methanococcus maripaludis and potential

biosynthesis
biosynthesis

... - FA are released into the circulation (albumin bound NEFA) - transport through the cell membrane ...
Altered Fermentative Metabolism in
Altered Fermentative Metabolism in

... anaerobic induction was performed and compared with immunoblots for PFL1 in the various mutant strains (Figure 3D). Based on this analysis, <2% of the PFL1 protein accumulated in pfl1-2 and pfl1-3 relative to wild-type cells (Figure 3D). Additionally a double mutant was generated by crossing a singl ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... fragment. The DNA sequence of the 200 bp fragment was determined by a primer designed according to the IS50 sequence of Tn5-233 (5⬘-CGGGAAAG GTTCCGTTCAGG-3⬘). The DNA sequencing data showed that transposon Tn5-233 is inserted into the 714th nucleotide of an open reading frame designated SMb21144, wh ...
BMC Bioinformatics
BMC Bioinformatics

Gene Technology Regulation 2004
Gene Technology Regulation 2004

... The status of this republication appears on the bottom of each page. Editorial changes The Legislation Act 2001, part 11.3 authorises the Parliamentary Counsel to make editorial amendments and other changes of a formal nature when preparing a law for republication. Editorial changes do not change th ...
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in

... in A. aeolicus and Tc. ruber. Based either on the whole genome sequence (A. aeolicus) or carbon isotopic measurements (Tc. ruber) both organisms were previously suspected to use this pathway, but direct evidence had been lacking (Deckert et al., 1998; Jahnke et al., 2001). Isocitrate dehydrogenase, ...
Biochemistry Ch 33 597-624 [4-20
Biochemistry Ch 33 597-624 [4-20

... membranes and blood lipoproteins -sources of glycerol 3-phosphate, which provides the glycerol moiety for triacylglycerol synthesis is DIFFERENT for liver and adipose -in LIVER, glycerol-3-phosphate is produced from phosphorylation of glycerol by glycerol kinase or from reduction of DHAP from glycol ...
Gel electrophoresis - Caltech Particle Theory
Gel electrophoresis - Caltech Particle Theory

... relative to their size or, for cyclic fragments, their radius of gyration. Single-stranded DNA or RNA tend to fold up into molecules with complex shapes and migrate through the gel in a complicated manner based on their tertiary structure. Therefore, agents that disrupt the hydrogen bonds, such as s ...
amino acid 1
amino acid 1

... 1. In peripheral tissues,the a-amino groups of the amino acids are transferred to glutamate by a transamination reaction, as in the liver. 2. However, rather than oxidatively deaminating glutamate to form ammonium ion, the a-amino group is transferred to pyruvate to form alanine. 3. The liver takes ...
Identification of loci affecting teat number by
Identification of loci affecting teat number by

... QTLs have been identified for this trait on almost all pig chromosomes except for SSC13, suggesting that teat number is controlled by multiple genes with generally small effects. We have previously identified 11 genome-wide significant QTLs for teat number by a genome scan on the White Duroc×Erhuali ...
8 VARIATION IN CHROMOSOME STRUCTURE AND NUMBER
8 VARIATION IN CHROMOSOME STRUCTURE AND NUMBER

... Figure 8.1c shows a human karyotype. The procedure for making a karyotype is described in Chapter 3 (see Figure 3.2). A karyotype is a micrograph in which all of the chromosomes within a single cell have been arranged in a standard fashion. When preparing a karyotype, the chromosomes are aligned wit ...
Composite Beef Breeds - FSA3057 - University of Arkansas Division
Composite Beef Breeds - FSA3057 - University of Arkansas Division

... composites is influenced by inbreeding. If no inbreeding is practiced, the heterosis is retained in composites for several generations, as shown in research results from the Meat Animal Research Center data (Table 1). Table 2 below demonstrates the theory behind heterosis retention in composite line ...
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3

... Helicobacter spp. [16]. However, among Campylobacter spp. it has been detected in only a subset of C. jejuni strains [17], and has shown a strong association with only certain C. jejuni STs [18,19]. The presence of GGT in C. coli has not been described before and opens a question concerning the real ...
Oral Candidiasis
Oral Candidiasis

... Though C. albicans is the most common cause of yeast infections, the non-albicans Candida species9 are increasing in prevalence. C. glabrata is the second most common species in candidiasis or candidemia10, and the number one non-albicans Candida spp. in blood stream infections (18). In patients you ...
File Ref.No.7054/GA - IV - J1/2013/CU  UNIVERSITY OF CALICUT
File Ref.No.7054/GA - IV - J1/2013/CU UNIVERSITY OF CALICUT

... Qualitative analysis Quantitative estimation Spotters ...
Table S2
Table S2

... Ace2: Homolog of Swi5 (see below), mutation of phosphorylation sites leads to change in nuclear localization; in vivo phosphorylation characterized in detail as part of this thesis[50] Ask1: Member of the DASH complex that binds to microtubules and kinetochores and regulates their association[69] Cd ...
Fatty acid synthesis in liver and adipose tissue
Fatty acid synthesis in liver and adipose tissue

... & Vagelos, 1976). As a consequence of studies carried out principally during the 1950s and 1960s with laboratory rodents, the adipose tissue has come to be regarded as a more important lipogenic tissue than the liver in non-lactating animals; Masoro et al. (1949) reported that fatty acid biosynthesi ...
Gene Section NOTCH2 (Notch homolog 2 (Drosophila)) Atlas of Genetics and Cytogenetics
Gene Section NOTCH2 (Notch homolog 2 (Drosophila)) Atlas of Genetics and Cytogenetics

Hydrolases as Catalysts for Green Chemistry and
Hydrolases as Catalysts for Green Chemistry and

... synthesis of a chiral pharmaceutical compound, S-clopidogrel, by selective hydrolysis of the racemic precursor. Current production of the pure S- clopidogrel isomer involves the use of a resolving agent, L-camphorsulfonic acid, and organic solvents. Screening of different hydrolases revealed that cr ...
HL7 V2.5.1 Genetic Test Result Message - HL7 Wiki
HL7 V2.5.1 Genetic Test Result Message - HL7 Wiki

Document
Document

... Once the amide nitrogen in compounds 1 or 4 gets deprotonated (Scheme 2) its nucleophilicity seems to be high and the formed five-membered ring is energetically favoured. As the chlorine in acyl chlorides 4 represents a good leaving group the reaction demands conditions only slightly above room temp ...
Amino acids
Amino acids

... an enzyme produced in hepatocytes and is highly concentrated in the liver. ...
"Yeast". - ResearchGate
"Yeast". - ResearchGate

... artificial chromosomes that can be used to study various aspects of chromosome behavior, such as how chromosomes pair and segregate from each other during mitosis and meiosis. In addition, systems using artificial chromosomes have been designed that allow cloning of larger contiguous segments of DN ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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