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Fibrous Proteins
Fibrous Proteins

... peptidase)-In most cases, the step of collagen synthesis which is disturbed in Ehlers-Danlos is the formation of cross-links  Mutation in the amino acid sequence of Collagen I,III or V  The replacement of glycine with another amino acid may lead to EDS  Glycine is used because it has the smallest ...
BACILLUS SPHAERICUS TOXINS: Molecular Biology and Mode of
BACILLUS SPHAERICUS TOXINS: Molecular Biology and Mode of

Serotype IX, a Proposed New Streptococcus agalactiae
Serotype IX, a Proposed New Streptococcus agalactiae

... Received 17 January 2007/Returned for modification 30 March 2007/Accepted 6 July 2007 ...
Glycerol is a major substrate for glucose, glycogen, and
Glycerol is a major substrate for glucose, glycogen, and

... embryos from small (56.6 ± 0.88 g) and large eggs (71.7 ± 1.09 g) were repeatedly dosed with either [13C3]glycerol (14 mg/d for 4 d) or [13C6]glucose (15 mg/d for 3 d) into the chorio-allantoic fluid before blood and tissue collection. 13C-Mass isotopomer enrichments in blood glucose, liver, and mus ...
Shigella boydii Involved in O-antigen Synthesis By
Shigella boydii Involved in O-antigen Synthesis By

... that the Glu101 residue within a DxD motif is essential for activity, possibly by forming the catalytic nucleophile. The Lys211 residue within a cluster of positively charged amino acids was also found to be required for activity. This latter residue may be involved in binding the negatively charge ...
Metabolism of acyl‐lipids in Chlamydomonas reinhardtii
Metabolism of acyl‐lipids in Chlamydomonas reinhardtii

... understanding of acyl-lipid metabolism in model microalgal species (Merchant et al., 2012; Liu and Benning, 2013). Historically, the green model microalga Chlamydomonas reinhardtii has not been the focus of many studies on fatty acid metabolism because it does not synthesize VLC-PUFAs and was consid ...
Is myeloma an inherited cancer?
Is myeloma an inherited cancer?

... Other information available from Myeloma UK Myeloma UK has a range of Essential Guides, Infoguides and Infosheets available covering many areas of myeloma, its treatment and management. To order your free copies or to talk to one of our Myeloma Information Specialists about any aspect of myeloma, c ...
Color Coat Genetics
Color Coat Genetics

... It is possible for the BROWN to express the ROAN gene. Usually this is considered as BLUE ROAN which is recognized as a separate coat color with AQHA. (See BLUE ROAN) A BROWN may have white hair scattered throughout the body or have white hair concentrated in specific areas. These areas are usually ...
Human mitochondrial transfer RNAs: Role of pathogenic
Human mitochondrial transfer RNAs: Role of pathogenic

... single-fiber polymerase chain reaction (PCR), and (5) cause defects of mitochondrial protein synthesis and respiratory chain deficiencies in single or multiple affected tissues of patients and demonstrable in cybrid cell lines. Cybrid cell lines are generated by transferring mitochondria isolated from ...
A New Cell Secreting Insulin
A New Cell Secreting Insulin

... enzyme. Oligo(deoxythymidine) primer (5⬘-GGAAGCTTTTTTTTTTTTTTTTTTT-3⬘), 10 ␮m dithiothreitol, 0.5 mm of each deoxynucleoside 5⬘-triphosphate, and the buffer supplied with the enzyme [containing 50 mm Tris-HCl (pH 8.3), 75 mm KCl, and 3 mm MgCl2] were added to the RT reaction mixture (final volume, 2 ...
A Mutation in LTBP2 Causes Congenital Glaucoma in
A Mutation in LTBP2 Causes Congenital Glaucoma in

... A Mutation in LTBP2 Causes Congenital Glaucoma in Domestic Cats (Felis catus). PLoS ONE 11(5): e0154412. doi:10.1371/journal.pone.0154412 Editor: Glyn Chidlow, Hanson Institute, AUSTRALIA Received: October 29, 2015 Accepted: March 31, 2016 Published: May 5, 2016 Copyright: This is an open access art ...
ETD Program - OhioLINK Electronic Theses and Dissertations Center
ETD Program - OhioLINK Electronic Theses and Dissertations Center

... Neurospora crassa is a fungus of the phylum Ascomycota, and was originally described as an orange mold that infested French bakeries in the 1800’s. The fungus was used in the early 1900’s by FAFC Went, a Dutch plant physiologist working in Indonesia and Surinam. This was because it was common to use ...
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human

... the nomenclature adopted at the 3rd Meeting of the European Study Group on Molecular Biology of Picornaviruses (Urbino, Italy, 1983) (Rueckert & Wimmer, 1984) has been used in this paper. Where reference is made to papers that used the earlier nomenclature, the former names of the respective protein ...
Microplate-Based Pathlength Correction Method for Photometric
Microplate-Based Pathlength Correction Method for Photometric

... Determining the liquid pathlength The liquid pathlength on a microplate well can be measured from water absorption at a near-infrared (near-IR) wavelength area. Figure 2 shows the absorption spectrum of water measured with either a 10 mm polyacrylic disposable cuvette or a 10 mm quartz cuvette. The ...
Benzimidazole resistance survey for Haemonchus - diss.fu
Benzimidazole resistance survey for Haemonchus - diss.fu

... assay using Caenorhabdithis elegans. All mutants successfully developing in the presence of the BZ benomyl were lacking functional ben-1 which encodes a b-tubulin in C. elegans (Driscoll et al., 1989). The first single nucleotide polymorphism (SNP) identified in a parasitic nematode correlating with B ...
From RNA to protein
From RNA to protein

... Genes to Phenotypes "A set of genes represents the individual components of the biological system under scrutiny" Modifications of the "3:1 F2 monohybrid ratio" and gene interactions are the rules rather than the exceptions" ...
calculating the structure-based phylogenetic relationship
calculating the structure-based phylogenetic relationship

... Dendrograms establish the evolutionary relationships and homology of species, proteins, or genes. The importance of dendrograms is demonstrated in numerous biological disciplines: They are utilized in homology modeling to ascertain which sequences are the most evolutionarily homologous to the query ...
Intestinal absorption of triglycerols
Intestinal absorption of triglycerols

... derives from many sources (diet, blood, digestive secretions, new synthesis in the enterocytes) and the triglycerides contain both endogenous and exogenous fatty acids. The endogenous lipids are derived from blood circulating through the intestinal mucosa, bile, desquamated intestinal epithelium cel ...
PDF
PDF

... The physiological activities of organs are underpinned by an interplay between the distinct cell types they contain. However, little is known about the genetic control of patterned cell differentiation during organ development. We show that the conserved Teashirt transcription factors are decisive f ...
Slide 1
Slide 1

... During sexual reproduction, male and female reproductive cells join in a process known as fertilization to produce a new cell. In peas, this new cell develops into a tiny embryo encased within a seed. ...
Attachment 2.2 Sequencing results
Attachment 2.2 Sequencing results

... fecundity and are defective in development of both liver and immune system. In these mice fetal vascular structures are found in the eyes, kidneys and liver. [1] Objective: to study the conservation of the AHR gene and investigating whether there is more than just one transcript of the AHR gene in t ...
Repression by RAZ of Epstein-Barr virus bZIP transcription factor
Repression by RAZ of Epstein-Barr virus bZIP transcription factor

... increasing amounts, the amount of FLAG-RAZ protein was far less than the amount of EB1 protein, even in the transfections where the amount of EB1 was reduced (Fig. 2c, compare mAbZ125 with mAbM2, lane 5). It should be stressed that 5,1 of total cell protein extract was used to visualize the amount o ...
invited review
invited review

... and the transcription factor vitamin D receptor (VDR), a direct effect on gene regulation. For efficiently applying the therapeutic and disease-preventing potential of 1,25(OH)2D3 and its synthetic analogs, the key steps in vitamin D signaling need to be understood. These are the different types of m ...
CD40/CD40L Dyad in the Inflammatory and Immune Responses in
CD40/CD40L Dyad in the Inflammatory and Immune Responses in

... critical role in the up-regulation of CD40 gene by IFN-γ in microglia (25), since co-treatment of microglia with IFN-γ and neutralizing antibodies to TNF-α attenuates the capacity of IFN-γ to induce CD40 mRNA expression. Further evidence for the involvement of TNF-α in IFN-γ induction of CD40 expres ...
Datasheet - Santa Cruz Biotechnology, Inc.
Datasheet - Santa Cruz Biotechnology, Inc.

... SANTA CRUZ BIOTECHNOLOGY, INC. ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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