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Intermediate Inheritance or Incomplete Dominance
Intermediate Inheritance or Incomplete Dominance

... • Avery and other scientists discovered that DNA is the nucleic acid that stores and transmits the genetic information from one generation of an organism to the next ...
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... 3. Gel electrophoresis separates DNA on the basis of size. 4. DNAs can be synthesized (up to ~100 bases commercially). (N) 5. PCR amplifies any target DNA sequence. (N) 6. Genes and genomes can be sequenced by chain termination. (N) 7. Oligonucleotides can be used to change bases by “site- directed ...
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Chapter 18 Reading Guide

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DNA and Protein Synthesis Concept Questions

... 8. If human DNA contains approximately 3x109 base pairs, and DNA polymerase can work at the rate of about 50 nucleotides per second, how can our DNA be replicated so quickly? 9. A certain chemical is known to fuse thymine with adenine in DNA. Comment on the possible effects of exposure to this chemi ...
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Unit 8.3: Biotechnology

... The polymerase chain reaction (PCR) makes many copies of a gene or other DNA segment. This might be done in order to make large quantities of a gene for genetic testing. PCR involves three steps: denaturing, annealing, and extension. The three steps are illustrated in Figure below. They are repeated ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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