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Genetics
Genetics

... • Transposons are small pieces of DNA that move readily from one site on bacterial chromosome to another or from bacterial chromosome to plasmid. • They carry antibiotic resistance genes. • Transfer of transposons on plasmids to other bacteria by conjugation contributes to antibiotic resistance. ...
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gene_expression_info

... 2. The first exposed mRNA codon is always AUG (start codon) 3. A tRNA molecule (with its aa -met) with an anticodon complimentary to the 1st codon lines up in position P 4. Complimentary base pairs form H bonds between the codon and anticodon (UAC with the AUG) 5. Another tRNA (Pro) complimentary ba ...
Chapter 2- Genetics
Chapter 2- Genetics

... • Each parent strand acts as a template to which ________________________ in cell can attach • Nucleotides attach themselves at their __________: adenine (A) with thymine (T), and cytosine (C) with guanine (G). • Enzymes,_____________, fuse free nucleotides together in complementary chain of DNA  F ...
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Topic 2 & 3: Genetics Review
Topic 2 & 3: Genetics Review

... 2.6.1 Compare the structure of RNA and DNA 2.6.2 Outline DNA transcription in terms of the formation of an RNA strand complementary to the DNA strand by RNA polymerase. 2.6.3 Describe the genetic code in terms of codons composed of triplets of bases. 2.6.4 Explain the process of translation, leading ...
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... By leveraging the same technologies used to design computer chips, Orchid is designing microchemical platform technologies capable of performing high-throughput chemical synthesis, biochemical assays, and DNA analysis for applications in drug discovery and diagnostics. Analogues/variants are availa ...
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DNA Technology

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< 1 ... 1977 1978 1979 1980 1981 1982 1983 1984 1985 ... 2254 >

Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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