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Evolutionary history of the genus Capra
Evolutionary history of the genus Capra

... One thousand bp of the last intron of ZFY were ampliWed using primers speciWcally designed for the Y-chromosome copy of the zinc Wnger gene: ZF2F: 5⬘-AAG ACC TGA TTC CAG GCA GTA-3⬘, and ZFY: 5⬘-CTT CTT ATT GGT AGT GTA GTA ATC-3⬘ (Lawson and Hewitt, 2002). The PCR was conducted using 45 ampliWcation ...
Expression analysis of Arc in mouse brain Theresa Köhler
Expression analysis of Arc in mouse brain Theresa Köhler

... Arc, an immediate early gene, expressed in the brain of mice encodes the activity regulated cytoskeletal-associated protein (ARC) that is found on dendrites of neurons. It regulates cell development and plays an important role in neuronal plasticity and formation of long-term memory. Arc is induced ...
Special aspects of renal metabolism
Special aspects of renal metabolism

Practice Questions, Lectures 6-13 (259 KB pdf file)
Practice Questions, Lectures 6-13 (259 KB pdf file)

... (c) The chi-square value is 20.5, two genotype classes with expected values <5 were combined together (the expected value was then >5). (d) Describe what the P value means in each of the cases where the null hypothesis was rejected. Question 9 Perform a chi-square goodness of fit test of Hardy Weinb ...
AP Biology Unit 4: Genetics - Chapter 14
AP Biology Unit 4: Genetics - Chapter 14

... • The first concept is that alternative versions of genes account for variations in inherited characters • For example, the gene for flower color in pea plants exists in two versions, one for purple flowers and the other for white flowers • These alternative versions of a gene are now called alleles ...
Tombola, a tesmin/TSO1-family protein, regulates
Tombola, a tesmin/TSO1-family protein, regulates

... Tomb. The Tomb-Aly and Tomb-Comr interactions were specific, as yeast co-transformed with Tomb-AD and CG15031-DB were unable to grow under selective conditions. Co-expression and coimmunoprecipitation experiments in tissue culture cells confirmed that FLAG-Tomb can interact with HA-Comr (data not sh ...
Amino Acids
Amino Acids

... II- Classification according to polarity of side chain (R): A- Polar amino acids: in which R contains polar hydrophilic group so can forms hydrogen bond with H2O. In those amino acids, R may contain: 1- OH group : as in serine, threonine and tyrosine 2- SH group : as in cysteine 3- amide group: as ...
Identification of Genes Mediating Drosophila Follicle Cell Progenitor
Identification of Genes Mediating Drosophila Follicle Cell Progenitor

... the third chromosome The general strategy for identifying genomic regions that are dosage sensitive for GAL4::UAS variegation is shown in Figure 1, B and C. We used two collections of largely isogenic strains bearing balanced heterozygous molecularly characterized deficiencies (also known as deletion ...
Sample pages 1 PDF
Sample pages 1 PDF

High Frequency in Vivo Loss of Heterozygosity Is Primarily a
High Frequency in Vivo Loss of Heterozygosity Is Primarily a

... the same colony-forming efficiency in DAP medium as in drug-free medium, indicating optimal selection conditions for APRT' clones. The 4 heterozygotes showed frequencies ranging from 21 X l0_6 to 154 X lO_6, a difference of about 7-fold. The frequencies of HPRT clones in the same individuals (except ...
Polymorphism of genes encoding PmrAB in colistin
Polymorphism of genes encoding PmrAB in colistin

... 20776– 1:2006. The threshold of antimicrobial resistance adopted in this work was based on the epidemiological cut-off (ECOFF) values recommended by EUCAST (http://www.eucast.org). The coding sequences of the pmrA and pmrB genes were amplified from crude DNA samples prepared by boiling cells in wate ...
Sheltie Colors General
Sheltie Colors General

... the "sable" dog bred to a dog with the merling gene, *It is important to underwhereas he may or stand color genetics. Some breeders may mistake this may not appeared double merle for a colorbrilliantly merled, a headed white. tri- or bi- dog bred to a merle that produces a blue merle become easily i ...
The Effects of Whey Supplementation and Natural Diet on Protein
The Effects of Whey Supplementation and Natural Diet on Protein

... Nutrition plays an important role in the promotion of training adaptations. According to The Institute of Medicine, 10-35% of daily calories for an average adult should come from protein, so active adults should be between 25-35%. The amount of protein that an athlete needs depends on a few factors: ...
Controlling Gene Expression in Bacteria
Controlling Gene Expression in Bacteria

... cells all the time. These continually expressed genes are called constitutive genes.  Other genes are only needed by certain cells or at specific times. The expression of these inducible genes is tightly controlled.  For example, pancreas beta cells make the protein insulin by expressing the insul ...
Enzyme Redundancy and the Importance of 2
Enzyme Redundancy and the Importance of 2

... organs and tissues. Whether these enzymes play overlapping (redundant) or distinct (non-redundant) roles is an important question. Recent data have begun to shed some light on this problem. This is relevant for different GS/GOGAT cycle enzymes but also when considering the enzymes producing 2OG, the ...
The Diagnosis of Mitochondrial Diseases
The Diagnosis of Mitochondrial Diseases

... Mitochondrial genetics is unusual in many ways. First, mitochondria and mtDNAs are unique in that they are inherited only from the mother. Thus, most pathogenic errors in mtDNA are maternally-inherited: women will transmit the defect to all of their children (males and females), but only the daughte ...
HMG CoA reductase
HMG CoA reductase

... Decrease intake of carbohydrate, saturated fatty acids & cholesterol Increase intake of mono- & polyunsaturated fatty acids Increase intake of fibers-rich diet ...
Endosymbiosis Theory
Endosymbiosis Theory

... such as Giardia lamblia, have neither mitochondria nor chloroplasts. They are all anaerobic. These facts suggest that mitochondria and chloroplasts do not appear to be intrinsic components of eukarya cells. Rather, they could be integrated into the cells of eukarya at a later evolutionary stage. In ...
Mendel Quiz 1. Who was Gregor Mendel? a) He was Charles
Mendel Quiz 1. Who was Gregor Mendel? a) He was Charles

... a) Pea plants have a short life cycle b) It is easier to breed pea plants c) Pea plants could be cross-pollinated and self-pollinated d) Pea plants produce pretty flowers 10) Which of the following was not a conclusion Mendel came up with at the conclusion of his experiments on pea plants? a) The in ...
Respiratory terminal oxidases in the facultative chemoheterotrophic
Respiratory terminal oxidases in the facultative chemoheterotrophic

... LE392, and HB101 were used for cloning, phage propagation, and conjugation, respectively. E. coli strains were grown in LB medium for cloning purposes and conjugation, and in NZY medium for phage propagation and plaque lifting. 2.2. Cloning, sequencing, and mutant construction A list of plasmids and ...
LE 5-2 - MrBrock.com
LE 5-2 - MrBrock.com

... • Steroids are lipids characterized by a carbon skeleton consisting of four fused rings • Cholesterol, an important steroid, is a component in animal cell membranes • Although cholesterol is essential in animals, high levels in the blood may contribute to ...
Gene Section SRXN1 (sulfiredoxin 1) Atlas of Genetics and Cytogenetics
Gene Section SRXN1 (sulfiredoxin 1) Atlas of Genetics and Cytogenetics

... The primary biochemical function of Srx is to reduce the overoxidized cysteine residues of Prx I, Prx II, Prx III and Prx IV under severe oxidative stress (Biteau et al., 2003; Chang et al., 2004). The spectrum and specificity of its enzymatic function remains elusive. Srx may also cause the degluta ...
using the crispr/cas9 gene editing tool to develop a cure for
using the crispr/cas9 gene editing tool to develop a cure for

... How CRISPR Acts as Molecular Scissors Due to the incredibly fast rate at which the CRISPR technology was adopted, the scientific community has quickly improved the use of CRISPR, although there are some variations in how it is used. The most important component is the CRISPR RNA molecule, which incl ...
mutations
mutations

... Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. Mutations are often thought of as negative because they disrupt the normal function of genes. However, without mutations, organisms cannot evolve, because mutations are the source of gen ...
MCDB 1041 Activity 4 Complex Patterns and Errors in Meiosis
MCDB 1041 Activity 4 Complex Patterns and Errors in Meiosis

... Chromosomal separation errors (non-disjunction) during meiosis are a common cause of miscarriages (approximately 10-20% of all human fertilized eggs contain chromosome abnormalities!); however, this is still a relatively rare event. Usually, during meiosis, chromosomes separate to produce gametes w ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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