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Downstream analysis of transcriptomic data
Downstream analysis of transcriptomic data

Biology – Chapter 17 Assessment Answers 17.1 Assessment 1a. A
Biology – Chapter 17 Assessment Answers 17.1 Assessment 1a. A

... 1a. A gene pool consists of all the genes, including all the different alleles for each gene that are present in a population. The allele frequency is the number of time that the allele occurs in a gene pool, compared with the number of times other alleles for the same gene occur. 1b. Change in the ...
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... pregnancy of the problem was associated with endometriosis. Rat experiments demonstrated that proper fetal development required a certain level of oestrogen. However at high levels it caused abnormalities. Thus administration of DES is more likely to cause developmental problems which usually result ...
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... 21. Be able to discuss genomic imprinting and its effects when inherited from mom or dad. (To help you understand this phenomenon, study Figure 14.9) 22. What is methylation? How does it contribute to our understanding of genomic imprinting and X-inactivation? 23. Remember from the chemistry section ...
Gene Regulation in Prokaryotic Cells
Gene Regulation in Prokaryotic Cells

... allosteric transition so that the repressor cannot bind the operator DNA anymore. G. A RNA polymerase binds to the promoter and turns on transcription. III. Discovery of the lac system of negative control A. Jacob and Monod found that genes were controlled together. H. Genetic evidence for the prese ...
Data Mining in Ensembl with BioMart
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DNA Function - Grayslake Central High School
DNA Function - Grayslake Central High School

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... Translation Translation is the process where ribosomes decode mRNA to produce amino acids. mRNA is decoded in three-base sections called codons. The codons code for one of 20 amino acids. There are 64 different codons (43 ) so several different codons can specify the same amino acid, or none at all ...
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... D. a large number of alleles of each gene E. none of the above 2. Which technique would be best for screening a large number of genes at the same time for detection of mutant genes? A. southern blotting D. PCR B. northern blotting E. DNA microarray C. western blotting 3. Enzymes that recognize a spe ...
Explain the difference between the following types of genome maps
Explain the difference between the following types of genome maps

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Temporal control of Transcription in phage SPO

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Synthetic Life - Colin Mayfield

... • M. mycoides JCVI-syn1.0 was transformed with a vector containing a selectable tetracycline-resistance marker and a b-galactosidase gene for screening • PCR experiments and Southern blot analysis of isolated putative transplanted cells • Multiple specific antibody reactions were carried out to test ...
< 1 ... 1915 1916 1917 1918 1919 1920 1921 1922 1923 ... 2254 >

Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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