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DNA and RNA
DNA and RNA

... If you unraveled all your chromosomes from all of your cells and laid out the DNA end to end, the strands would stretch from the Earth to the Moon about 6,000 times. ...
RNA - Gulf Coast State College
RNA - Gulf Coast State College

... attaches them to the growing protein chain 3) When protein production is complete, the ribosome releases the protein chain ...
RNA - Gulf Coast State College
RNA - Gulf Coast State College

... attaches them to the growing protein chain 3) When protein production is complete, the ribosome releases the protein chain ...
4.Genetechnology2
4.Genetechnology2

... Developing suitable methods for locating and isolating genes of interest is an important part of gene technology There are three main methods for obtaining genes • Synthesising the gene using an automated gene machine – this method can be used if the amino sequence of the protein gene product is kno ...
DNA Extraction Laboratory
DNA Extraction Laboratory

... the tube. The alcohol should form a layer on the surface of the solution in the tube. Add cold alcohol until the tube in half full. Do not shake or mix the tube. 6. A white precipitate should start to appear at the interface of the solution and the alcohol. This is DNA! 7. Use a wooden stick to pull ...
Slide 1
Slide 1

... Coding region – contains nucleotide sequence that encodes a specific protein product (this region will be translated) In eukaryotes: introns and exons Non-coding regions – contains nucleotide sequence that will get transcribed BUT not translated *Un-translated regions (UTR’s) Promoter regions – sequ ...
1. Name the two major divisions of metabolism, and
1. Name the two major divisions of metabolism, and

... Explain why an enzyme that catalyzes a step in glycolysis would not be required for a step in Beta-oxidation (i.e. fat metabolism) Because enzymes are specific for their substrates. 15. Construct a molecule of DNA. Be sure to label parts fully (if using abbreviations, make sure to provide a key). ...
Gene expression
Gene expression

... prevention to a person’s genotype. • Commercial tests for athletic potential are available, based on the R and X alleles of the ACTN3 gene. • XX genotype is unusually common in endurance athletes (24 percent), but rare in strength-sport athletes, who are more likely to be RR than all others. • Knock ...
AP review
AP review

... Sequence specific recognition of DNA by proteins. • Nitrogen and oxygen exposed in the grooves can make hydrogen bonds with proteins. • Different Watson/Crick base pairs have different patterns of donors and acceptors ...
Ch5hybridisationSNPRFLP
Ch5hybridisationSNPRFLP

... Methods of genetic manipulation are named: • Recombinant DNA technology • Genetic engineering • Gene cloning or gene technology Applications include: • Isolation of specific genes • Production of specific proteins ...
microbiology exam i - Medical Mastermind Community
microbiology exam i - Medical Mastermind Community

... the new bacterial species and infect the new bacterial species. 28. You would like to design a diagnostic test for the 3 base-pair deletion that causes cystic fibrosis. What would be a reasonable method and efficient method? A. Clone the entire CF gene from patients and determine the DNA sequence of ...
Unit 5: Hypercholesterolemia Section 1: Cholesterol A lipid that
Unit 5: Hypercholesterolemia Section 1: Cholesterol A lipid that

... an increased risk of heart attack & coronary heart disease, & that is inherited as an autosomal dominant trait. Having 2 different alleles for a given gene. Having 2 identical alleles for a given gene. Relating to a straight line or capable of being represented by a straight line. A rare change in t ...
Protein
Protein

... 4th phase of Mitosis  Chromosomes begin to uncoil back into chromatin state. – Cytokinesis • Cytoplasm divides • Two new daughter cells are now separate  2 nuclear membranes form around each set of chromosomes.  Nucleolus forms in each of the newly formed cells. ...
Gene and Genome Evolution
Gene and Genome Evolution

... generation. This is due to a direct correlation between copy number and age of onset. • There is a genetic test for the disease, but in the absence of effective treatment few actually take the test. • Function of the protein remains unknown, the excess glutamines cause it to aggregate and (probably) ...
Brainpop Genetics questions Weinmann ANSWERS
Brainpop Genetics questions Weinmann ANSWERS

... 2. Which of the following depicts (shows) a molecule of DNA? - B 3. What is the relationship between DNA and chromosomes? - A. chromosomes are made out of DNA 4. How many PAIRS of chromosomes exist in each of your cells? - D. 23 pairs (46 individual) 5. Why are your chromosomes arranged in pairs? - ...
03oligomicroarray
03oligomicroarray

... • In one approach, sequences are synthesized on a slide using ink-jet technology similar to that used in color printers. Separate cartridges for the four bases (A, C, G, T) are used to build nucleotides on a slide. • The company Affymetrix uses a photolithographic approach which we will describe bri ...
Outline Section 1
Outline Section 1

... a. The number of bases and the complexity of their organization vary far more than the number of genes b. The conservation of critical functions and the base sequence of the genes that code for them show that all cells are related evolutionarily c. These close structure and function relationships al ...
Transcription
Transcription

... • The copying of DNA-encoded genetic information into RNA is known as transcription (TC), with the further conversion into protein being termed translation (TL). This concept of information flow is known as the Central Dogma of molecular biology and is an underlying theme in all studies of gene expr ...
The Nature of Bacteria
The Nature of Bacteria

... 4. Conjugative plasmids contain the genes for transfer 5. Conjugation may cross species lines 6. Nonconjugative plasmids transferred by plasmid mobilization 7. Many plasmid genes promote survival and pathogenesis 8. In absence of selection pressure plasmids may be lost a. Conjugation in Gram-Negativ ...
DNA Sequence Analysis for Epilepsy and Seizure
DNA Sequence Analysis for Epilepsy and Seizure

DNA - Gulf Coast State College
DNA - Gulf Coast State College

... RNA is a copy of DNA that goes out into the cytoplasm to tell the cell what to do in order to stay alive  RNA: ribonucleic acid  You can always make more ____ so it’s ok if it gets destroyed (You can’t make more ____!!!) DNA RNA How many ____ ___ strands? Nucleotide subunit ...
Genome sequencing, assembly and annotation
Genome sequencing, assembly and annotation

... How many scaffolds do you end up with, and what is their total length? Optional: how many reads are there originally, and how long are they in total? What is the average read coverage of the BAC? Optional: try some different ‘error rates’ in each of the assembly steps to see how these influence the ...
Mutations in the code
Mutations in the code

... • (0.1% difference means 99.9% identical) • We have about 3 billion nucleotides in all, so that means there are about 3 million nucleotide differences between 2 people ...
Errors in Genes and Chromosomes
Errors in Genes and Chromosomes

... During DNA replication, an incorrect base may be added to the growing polynucleotide chain. DNA Polymerase I performs a proofreading function. When a mispairing of bases occur during the replication process, then the enzyme will remove the improperly placed base and try again. (Helicase, DNA ligase ...
Gene movement in bacteria Transformation Bacteria that undergo
Gene movement in bacteria Transformation Bacteria that undergo

... Specific 9-11 bp sequence in dsDNA required for binding to competent cell (5’ AAGTGCGGT 3’). ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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