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The role of humans in facilitating and sustaining coat
The role of humans in facilitating and sustaining coat

... characteristic. In 1959 he began testing his hypothesis by breeding silver foxes, an animal never previously domesticated. He selected against aggression and only allowed the tamest 10% of the population to breed. Belyaev expected that by selecting a specific behavioural trait he would influence the p ...
Evolutionary Potential and Requirements for Minimal Protocells
Evolutionary Potential and Requirements for Minimal Protocells

... organisms consist of units that are living systems even if the multicellular organism is killed. Conversely, the death of many of its cells does not necessarily kill the organism. If we are interested in the origin of life, our ultimate target must be the explanation of the origin of the prokaryotic ...
CitA (citrate) and DcuS (C 4 -dicarboxylate) sensor kinases in
CitA (citrate) and DcuS (C 4 -dicarboxylate) sensor kinases in

A disproportionate role for mtDNA in DobzhanskyMuller
A disproportionate role for mtDNA in DobzhanskyMuller

... ancient endosymbiosis, in this case between a heterotrophic cell and a photosynthetic symbiont. As in the mitochondrial case, most of the proto-chloroplast genome has been transferred to the host nuclear genome; approximately 90% of the proteins required for chloroplast function are imported nuclear ...
Bacteriophage MS2 RNA
Bacteriophage MS2 RNA

... particular, at least in E. coli, in that the tRNAArg contains an inosine instead of a G in the wobble position. It seems, therefore, that in the case of intrinsically weak codon-anticodon interaction, involving A and/or U in position I and 2, it is the strong, non-wobble codon which is strongly favo ...
Interrogating the Function of Metazoan Histones using
Interrogating the Function of Metazoan Histones using

... are 7-fold more abundant in wild-type flies than 24x-Rescue flies (Figure 2B). These experiments indicate that the haploid Drosophila genome contains approximately 100 histone repeats. Second, we calculated the histone gene copy number using high-throughput sequencing analysis. We reasoned that the ...
PDF
PDF

... pCR2.1-ACF1-flag using the Site-directed Mutagenesis Kit (Stratagene). The following pairs of oligonucleotides were used to delete the PHD1 or PHD2, respectively: PHD1F, 5⬘-ACCAATAAGTCATTAGTCGACGTAAAGAGTCTGGGTCTCAGC-3⬘; PHD1R, 5⬘-ACCCAGACTCTTTACGTCGACTAATGACTTATTGGTGGAACGC-3⬘; PHD2F, 5⬘-GATGAGGAAAAG ...
Product Information FluoroVueTM Nucleic Acid Gel Stain (10,000X
Product Information FluoroVueTM Nucleic Acid Gel Stain (10,000X

... The staining dye can be added into the electrophoresis buffer at a 1:10000 dilution for gel staining during electrophoresis. During/after electrophoresis the gel should be protected from light. The sensitivity of this method is slightly lower than the In-Gel Staining method. 3. Staining after electr ...
Metabolomics of Genetically Modified Crops
Metabolomics of Genetically Modified Crops

... Current MS-based approaches yield higher sensitivity than NMR when analyzing complex plant metabolite mixtures. The use of high and ultra-high resolution mass spectrometers greatly improves analytical performance and offers the best combination of selectivity and sensitivity [20,21]. However, to ach ...
The complete nucleotide sequence of apple mosaic virus (ApMV
The complete nucleotide sequence of apple mosaic virus (ApMV

... bromoviruses and cucumoviruses have been available for some time (Brunt et al., 1996), it was only recently that any complete ilarvirus sequences were published (Scott & Ge, 1995 ; Ge et al., 1997 ; Scott et al., 1998). This is probably due in part to the difficulty in handling and manipulating ilar ...
PDF
PDF

... of a focused light beam [12, 13]. Soon thereafter, magnetic tweezers were invented to allow the manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their o ...
Meiosis
Meiosis

... and thicken ...
Close - Journal of Integrated OMICS
Close - Journal of Integrated OMICS

... ciprofloxacin; streptomycin; gentamicin; and kanamycin) was tested. Enterococcus faecalis ATCC 29212 and Staphylococcus aureus ATCC 25923 strains were used for quality control. Similarly, susceptibility of the E. coli isolates was performed to 16 antimicrobial agents [ampicillin, amoxicillin plus cl ...
Characterization of lysine decarboxylase
Characterization of lysine decarboxylase

... locus in Escherichia coli. Inactivation of cadC by an insertion sequence has been reported to also cause LDC negativity in E. coli (Casalino et al., 2003). PCR was used to detect the cadBA operon and the cadC gene of the LDC-negative strains. The results showed that bands with the expected sizes wer ...
co-existence more politics than science
co-existence more politics than science

...  only breeding based on recombinant DNA generates risk to biosafety; all others are safe and need no regulation. ...
Variation in the size of the oqhcontaining linear
Variation in the size of the oqhcontaining linear

... We have found that the three recently defined Borrelia species associated with Lyme disease have differences in the sizes of their ospA-containing plasmids but not their linear chromosomes. The ospA-containing plasmid from B. burgdorferi was statistically smaller than the corresponding DNA molecules ...
Unit F215 - Control, genomes and environment - Medium band
Unit F215 - Control, genomes and environment - Medium band

... OCR has produced these candidate style answers to support teachers in interpreting the assessment criteria for the new GCE specifications and to bridge the gap between new specification release and availability of exemplar candidate work. This content has been produced by senior OCR examiners, with ...
Supplementary Material
Supplementary Material

... containing hlh-17 and hlh-31, nor a 9 kb subclone of this cosmid containing both genes, was able to rescue the larval lethality (data not shown). Next, we examined animals heterozygous for the ok487 allele and a deficiency, sDf23, which deletes the hlh-17/hlh-31 locus (data not shown). We found that ...
Statistical Analysis of Amino Acid Patterns in Transmembrane
Statistical Analysis of Amino Acid Patterns in Transmembrane

... To ®nd motifs that mediate helix-helix interactions in membrane proteins, we have analyzed frequently occurring combinations of residues in a database of transmembrane domains. Our analysis was performed with a novel formalism, which we call TMSTAT, for exactly calculating the expectancies of all pa ...
Rotavirus future surveillance activities and nested studies:
Rotavirus future surveillance activities and nested studies:

... These may be identified as vaccine failures in countries with high vaccination coverage, and may be identified simply by the addition of data relating to vaccination collected through EuroRotaNet strain surveillance: Currently data collection forms include fields for this information, but systems ar ...
Mendels Genetics
Mendels Genetics

... 1. Gene located on either the X or Y chromosomes 2. Females have 2 X chromosomes so rarely show the recessive phenotype; males have just 1 X chromosome so will show the trait for a single recessive allele for genes on the X chromosome 3. If find a trait that is more common in males than females it i ...
Genome Visualisation and Annotation Tools: Artemis and ACT
Genome Visualisation and Annotation Tools: Artemis and ACT

... Feature. A window will appear containing all the annotation that is associated with that CDS. The format for this information is constrained by that which can be submitted to the EMBL database where it is stored within ‘keys’ and ‘Qualifiers’ see Appendix II. Viewing amino acid or protein sequence C ...
Ch 11 Mendelian Patterns of Inheritance
Ch 11 Mendelian Patterns of Inheritance

... Rabbits have a gene that codes for darker pigments - this gene is more active at low temperatures. Parts of the body that are colder will develop the darker pigmentation ears and feet ...
Set - people.vcu.edu
Set - people.vcu.edu

... Anab7120:alr1407 nifV1 homocitrate synthase Anab7120:asr1408 nifZ ...
Analysis of Genetic Toggle Switch Systems Encoded on Plasmids
Analysis of Genetic Toggle Switch Systems Encoded on Plasmids

... ing time , namely, the average time between spontaneous transitions. Two variants, the general switch and the exclusive switch, were studied and compared in Refs. [27]. The difference between these two variants is that in the exclusive switch there is an overlap between the two promoter sites, so t ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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