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Hamster Placental Lactogen-ll Contains a Structural Feature Unique
Hamster Placental Lactogen-ll Contains a Structural Feature Unique

... To obtain cDNAs for haPL-ll, a A expression library was constructed using cDNA synthesized from RNA of day16 pregnant hamster placenta. Five positive clones were obtained from immunoscreening of 1 x 105 clones using a polyclonal antiserum to haPL-ll (6). Two clones with cDNAs of approximately 800 ba ...
Two Arabidopsis Genes (IPMS1 and IPMS2
Two Arabidopsis Genes (IPMS1 and IPMS2

... structure (dimer and tetramer, respectively). A knockout insertion mutant for IPMS1 showed an increase in valine content but no changes in Leu content; two insertion mutants for IPMS2 did not show any changes in soluble amino acid content. Apparently, in planta each gene can adequately compensate fo ...
Excess of charged tRNA maintains low levels of peptidyl
Excess of charged tRNA maintains low levels of peptidyl

... from the restored capacity of the cells to grow at 41 C that the presence of Pth(Ts) protein at levels compatible with viability should continue indefinitely. In AA7852 cells incubated at 32 C, neither the level (Figure 1B) nor the halflife of Pth(Ts) changed with tRNALys overproduction (data not ...
Scientists Complete Genome Sequence of Castor Bean Beans also
Scientists Complete Genome Sequence of Castor Bean Beans also

... A team of researchers from the Institute for Genome Sciences (IGS) at the University of Maryland School of Medicine and the J. Craig Venter Institute (JCVI) have sequenced and analyzed the genome of the Castor bean (Ricinus communis). The castor bean is the source of the highly valuable castor oil, ...
paper describing HSSP
paper describing HSSP

... sequence pattern characteristic of a protein family. Statistical methods are less successful but more general and are based on the derivation of structural preference values for single residues, pairs of residues, short oligopeptides or short sequence patterns. Both kinds of methods are severely lim ...
Page 4 of 4 - Thermo Fisher Scientific
Page 4 of 4 - Thermo Fisher Scientific

... components for commercial purposes. The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any commercial purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any ...
Meiosis Notes
Meiosis Notes

... frequency of crossing-over between genes during meiosis might be a clue to the genes’ locations. Sturtevant reasoned that the farther apart two genes were on a chromosome, the more likely it would be that a crossover event would occur between them. If two genes are close together, then crossovers be ...
Complex Signatures of Natural Selection at the Duffy Blood Group
Complex Signatures of Natural Selection at the Duffy Blood Group

The Promises and Pitfalls of Genoeconomics
The Promises and Pitfalls of Genoeconomics

Plasma membrane - HCC Learning Web
Plasma membrane - HCC Learning Web

... • Contents of the Nucleus • DNA ...
Phylogenetic Motif Detection by Expectation
Phylogenetic Motif Detection by Expectation

... In order to test the generality of the results achieved for the S. cerevisiae S. mikatae alignments, we calculated the log ratio of the likelihood of the evolutionary mixture using the real Gcn4p matrix to the polyT matrix over a range of evolutionary distances and rates of evolution (figure 2, fill ...
development of bioresponsive devic
development of bioresponsive devic

... based on the use of a self-foldable duplex or quadruplex DNA architecture. Briefly, for this strategy is placed heavily folded single stranded DNA duplexes in the pore vicinity, which are able to block the diffusion through the pores. Hence, the exposure of those duplexes to an adequate stimulus indu ...
Genetic mapping of aphicarus – a sex-linked locus
Genetic mapping of aphicarus – a sex-linked locus

... represent well-studied examples of alternative phenotypes (Harrison, 1980; Roff, 1986; Zera and Denno, 1997). Insects across a wide range of taxa have evolved the capacity to develop flight-capable (fully winged) phenotypes or flight-incapable (wingless or short-winged) phenotypes. The winged phenot ...
THE EFFECT OF INBREEDING ON THE VARIATION DUE TO
THE EFFECT OF INBREEDING ON THE VARIATION DUE TO

... genes which do not act additively, there is not the same correspondence lietween heterozygosity and variance and the above relationships do not hold. A s we know little about the dominance relationships of the genes controlling continuous variation, it seemed desirable to investigate theoretically t ...
Genome organization of Magnaporthe grisea
Genome organization of Magnaporthe grisea

... a mortar and pestle in the presence of sand. The mycelial powder was mixed with 3 ml of hot (65°C) lysis buffer (0.5 M NaCl; 10 mM Tris-HCl, pH 7.5; 10 mM EDTA; 1% SDS) and 0.3 ml of 10% CTAB in 0.7 M NaCl to form a slurry, and then 3.0 ml of phenol/ chloroform/isoamylalcohol (25 : 24 : 1, vol) was ...
Regional Differences in Protein Synthesis within the Lens of
Regional Differences in Protein Synthesis within the Lens of

... .he ocular lens is unique among mammalian organs in that it is wholly composed of a single, epithelial cell type. Despite continued production of new cells, older cells are not shed. Instead, they are incorporated directly into the body of the lens itself. Thus, it is a population of cells character ...
17. Prof. K. P. Bhatia: Paroxysmal Movement Disorders
17. Prof. K. P. Bhatia: Paroxysmal Movement Disorders

... attacks usually unilateral, frequently manifest as dystonia and are induced by a sudden change in position, classically from a sitting to standing. However, even startle, continuous exercise or a sudden change in velocity can trigger them and, rarely, they can occur in sleep. A preceding ‘aura-like’ ...
Hexose Monophosphate Shunt (HMP Shunt)
Hexose Monophosphate Shunt (HMP Shunt)

... HMP shunt (PPP) is less active in skeletal muscle & non-lactating mammary glands Site:- ...
Chapter Five - DORAS
Chapter Five - DORAS

... The entire foxB gene was amplified from P. aeruginosa PAO1 genomic DNA using the forward primer foxBF1 and reverse primer foxBR1 (figure 5.4). The forward and reverse primers were designed to incorporate an EcoRІ site and a PstІ site respectively into the PCR product. The reverse primer was designed ...
Comparative Genomics
Comparative Genomics

... We can better understand evolution/ speciation We can find important, functional regions of the sequence (codons, promoters, regulatory regions) It can help us locate genes in other species that are missing or not welldefined (also through comparison and alignments). Quality control! ...
Chapter 24 Translation
Chapter 24 Translation

... 24.15 Termination Codons Are Recognized by Protein Factors • Termination codons are recognized by protein release factors, not by aminoacyltRNAs. • RF1 – The bacterial release factor that recognizes UAA and UAG as signals to terminate polypeptide translation. • RF2 – The bacterial release factor th ...
cell culture mycoplasmas
cell culture mycoplasmas

... 33258 stain). This assay is relatively easy and rapid to perform (8). But the results are sometimes difficult to interprete and some experience is definitely necessary. Especially when the cell culture is not in a good condition, mis-interpretations are frequent. The sensitivity and specificity of t ...
draft safety assessment report - Food Standards Australia New
draft safety assessment report - Food Standards Australia New

... FSANZ has prepared a Draft Assessment Report of Application A509; and prepared a draft variation to the Australia New Zealand Food Standards Code (the Code). FSANZ invites public comment on this Draft Assessment Report based on regulation impact principles and the draft variation to the Code for the ...
Gene Section BAX (BCL2-associated X protein) Atlas of Genetics and Cytogenetics
Gene Section BAX (BCL2-associated X protein) Atlas of Genetics and Cytogenetics

... pancreatic cancer cells to apoptosis induced by chemotherapeutic agents. In the case of stage II colon cancer, treated only with surgery, BAX protein expression may be a predictor for prognosis. In ovarian cancer, BAX protein may have a predictive potential in taxane-platinum-treated patients. Moreo ...
Understanding fatty acid synthesis in developing - Shachar
Understanding fatty acid synthesis in developing - Shachar

... Smith et al., 1992; Kleppinger-Sparace et al., 1992; Neuhaus et al., 1993; Kang and Rawsthorne, 1996). The ATP necessary for fatty acid synthesis in non-photosynthetic tissues can be produced inside the plastid during the synthesis of acetyl-CoA from glucose 6-P and PEP (Boyle et al., 1990; Klepping ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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