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Microbial Cause of Calcium Lactate Defect in Cheddar Cheese
Microbial Cause of Calcium Lactate Defect in Cheddar Cheese

... Kochhar, S., H. Hottinger, N. Chuard, P. G. Taylor, T. Atkinson, D. Scawen, and D. J. Nicholls. 1992b. Cloning and Overexpression of Lactobacillus helveticus D-Lactate Dehydrogenase Gene in Escherichia coli. European Journal of Biochemistry. 208:799-805. Marshall, R. T. 1992. Standard Methods for th ...
Chapter 15 ppt - Bremen High School District 228
Chapter 15 ppt - Bremen High School District 228

Epidemiology and control of fungally transmitted cereal viruses
Epidemiology and control of fungally transmitted cereal viruses

... barley mild mosaic virus (BaMMV) and barley yellow mosaic virus (BaYMV) and their fungus vector, Polymyxa graminis. This includes studies of vector biology, variability and control; the viruses and their variability; methods for diagnosis of virus in soil and the nature of cultivar resistance. Studi ...
Inheriting Genetic Conditions Handbook
Inheriting Genetic Conditions Handbook

... by mutations in a single gene, such as cystic fibrosis and sickle cell anemia. While a family medical history provides information about the risk of specific health concerns, having relatives with a medical condition does not mean that an individual will definitely develop that condition. On the oth ...
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... The National Center for Biotechnology Information (NCBI) is a division of the National Library of Medicine (NLM) at the National Institutes of Health (NIH). The NCBI maintains many enormous databases, including GenBank, which stores all known public DNA sequences, submitted from individual scientist ...
An essential cell cycle regulation gene causes hybrid inviability in
An essential cell cycle regulation gene causes hybrid inviability in

... We recovered 32 viable hybrid F1 males from these crosses (compared with >300,000 hybrid F1 females). Of these, 26 males were the result of a nondisjunction event that led to their inheriting a D. simulans X-chromosome (11); these males were viable, as shown previously (2). Further tests confirmed t ...
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Regulation of Arabidopsis 14-3

... Abscisic acid (ABA) and ethylene signalling are important in responses to stress and in the regulation of primary metabolism in Arabidopsis seedlings (Gazzarrini & McCourt 2001). Since GABA might be involved in responses to stress and/or nutrients, we were interested to test whether these signalling ...
Evolutionary dynamics of autosomal-heterosomal rearrangements in
Evolutionary dynamics of autosomal-heterosomal rearrangements in

... rearrangements involving a sex chromosome and an autosomal pair, producing chiasmatic sex chromosomes. For example, a fusion of the X chromosome with an autosome will produce an expanded X chromosome while the remaining sister chromosome will become an additional sex chromosome. In XY systems, this ...
PPT - Altogen Biosystems
PPT - Altogen Biosystems

... 3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
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...  Allele Segregation Activity ...
Genes involved in asexual sporophyte development in Ceratopteris
Genes involved in asexual sporophyte development in Ceratopteris

... The life cycle of land plants involves cycling between two multicellular phases, the haploid gametophyte and the diploid sporophtye. Asexual reproductive strategies, that bypass meiosis and fertilization, have evolved in many plants representing diverse taxa among the land plant phylogeny. Apogamy i ...
A modular approach to sphingolipid analogs mediated by aziridines: Synthesis
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... Sphingolipid  structures  are  defined  by  their  eighteen  carbon  backbones  with  a  2‐amino‐1,3‐ diol functionality (usually 2S, 3R), which are called sphingoid bases. These organic bases can be  N‐acylated  by  fatty  acids  of  different  length  giving  ceramides.  Modification  of  this  ge ...
Pr-P-105
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... then decreased, especially for TBG. Many studies confirmed that enhanced UV-B radiation exerted an influence on physiological and biochemical process of plants and plants were injured by excessive dosage of UV-B, during which expression of genes relating to plant self protections was accelerated, re ...
An Analysis of the Arabidopsis Pollen
An Analysis of the Arabidopsis Pollen

... Becker et al. (Becker et al., 2003) that used ATH8 microarrays covering about one-third of the Arabidopsis genome. In both studies, viable, hydrated pollen grains were sorted from other pollen stages and cell debris with flow cytometry. Using ATH1 microarrays, representing 22,750 annotated genes, Pi ...
Familial subepithelial corneal amyloidosis
Familial subepithelial corneal amyloidosis

... analysis. The protein was identified by amino acid sequencing, Western blotting, and immunohistochemistry. Results. A protein was identified in two corneas with FSCA that was not present in normal corneas or in corneas with other disorders. The amino acid sequences of two peptides derived from this ...
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The biased nucleotide composition of the HIV genome: a constant

... composition has implications for the amino acids that are encoded by the open reading frames (ORFs), both in the requirement of specific tRNA species and in the preference for amino acids encoded by e.g. A-rich codons. Nucleotide composition does obviously affect the secondary and tertiary structure ...
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Biomereology_Montrea.. - Buffalo Ontology Site

... Each (clinical, pathological, genetic, proteomic, pharmacological …) information system uses its own classification system How can we overcome the incompatibilities which become apparent when data from distinct sources needs to be combined? ...
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1 Synthesis, Characterization, and Biological Reactivities of Metal

... V(saphen) (green, 95% yield), Cr(saphen) ( dark yellow, 50% yield), Mn(saphen) (brown, 70% yield), Fe(saphen) (black-brown, 80% yield), Co(saphen) (light brown, 75% yield), Ni(saphen) (orange, 70% yield), Cu(saphen) (green, 90% yield), Zn(saphen) (yellow, 50% yield). Al(saphen) can also be produced ...
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Genes Critical for Muscle Development and Function in

... membrane protein B-integrin (Gettner, S., C. Kenyon, L. Reichardt, J. Plenefisch, M. B. Buchner, and E. Hedgecock. 1992. Mol. Biol. Cell. 3:1088a), indicating the importance of the ECM and cell membrane in lattice assembly. Among the latter are genes which may code for tropomyosin and troponin C. Th ...
manualE6901
manualE6901

... the NdeI and SapI sites. The target protein is fused at its C-terminus to a selfcleavable intein tag (~28 kDa) that contains the chitin binding domain (CBD, 6 kDa) allowing for affinity purification of the fusion precursor on a chitin column. The pTYB21 (NEB #N6701) vector utilizes an intein from th ...
Wright, Sewall Evolution in Mendelian populations. Genetics, 16:97
Wright, Sewall Evolution in Mendelian populations. Genetics, 16:97

... There may be equilibrium here, if s, and sb are alike in sign, and s,b is either opposite in sign or of the same sign and smaller, but it is an unstable equilibrium. Of more general importance, perhaps, is the equilibrium reached by a deleterious mutant gene. For mutation opposed by genic selection ...
Microbiology
Microbiology

... digested and cloned in plasmid pSUP102. After ligation, D N A was transformed in E. coli DH5a (Bethesda Research Laboratories). Km-resistant colonies were selected and analysed for plasmid content. Plasmid clones with the T n 5 insertion were designated pIS14 and pIS50. DNA sequencing. The left junc ...
Open the publication - UEF Electronic Publications
Open the publication - UEF Electronic Publications

... detected from the genes showing the largest differences. This, however, proved to have technical and biological limitations, resulting in false biological interpretations at its worst1–3. Gene sets were introduced to overcome the challenges that focusing to a few genes brought up. Gene sets are grou ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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