FEBS Letters
... As a ¢rst step towards the identi¢cation of the gene encoding Dxr, the characteristic enzyme for the MEP pathway, a suitable gene probe was developed. The deduced dxr sequence of E. coli (P45568) was therefore compared to the hypothetical Dxr-like protein sequence of the sequenced genome of Synechoc ...
... As a ¢rst step towards the identi¢cation of the gene encoding Dxr, the characteristic enzyme for the MEP pathway, a suitable gene probe was developed. The deduced dxr sequence of E. coli (P45568) was therefore compared to the hypothetical Dxr-like protein sequence of the sequenced genome of Synechoc ...
PKC modulates NF-B and AP-1 via mitogen
... the p54/p46 c-Jun NH2-terminal kinases (JNKs), are important upstream regulators for the induction of various transcription factors (12, 37, 44). The role of each MAPK subfamily in the activation of transcription factors appears to be cell-type specific. In noncardiac cells, it has been shown that p ...
... the p54/p46 c-Jun NH2-terminal kinases (JNKs), are important upstream regulators for the induction of various transcription factors (12, 37, 44). The role of each MAPK subfamily in the activation of transcription factors appears to be cell-type specific. In noncardiac cells, it has been shown that p ...
Metabolism of Mollicutes: the Embden-Meyerhof
... where indicated. In assays where no enzyme and substrate concentrations ranged from 160-640 p ~ except activity was detected, 0.1-5.0 IU of the commercially available purified enzyme being studied was added directly to the apparently negative complete reaction mixture. Protein concentrations in the ...
... where indicated. In assays where no enzyme and substrate concentrations ranged from 160-640 p ~ except activity was detected, 0.1-5.0 IU of the commercially available purified enzyme being studied was added directly to the apparently negative complete reaction mixture. Protein concentrations in the ...
The rate of glycolysis quantitatively mediates specific
... leaving little room for biosynthesis [10]. Also, the rapid ATP produced by glycolysis can also be obtained by other mechanisms such as creatine kinase and adenylate kinase [11, 12]. Additionally, direct biochemical signaling functions of glucose metabolism are also possible [13, 14]. When the rate o ...
... leaving little room for biosynthesis [10]. Also, the rapid ATP produced by glycolysis can also be obtained by other mechanisms such as creatine kinase and adenylate kinase [11, 12]. Additionally, direct biochemical signaling functions of glucose metabolism are also possible [13, 14]. When the rate o ...
Environmentally Sensitive Fluorescent Sensors Based on Synthetic Peptides Linköping University Post Print
... Half of the reported protein biosensors are based on bacterial periplasmic binding proteins and target sugars, the reason being that these proteins are well described and that sugar binding induces a pronounced conformational change that may result in local changes in the chemical environment in man ...
... Half of the reported protein biosensors are based on bacterial periplasmic binding proteins and target sugars, the reason being that these proteins are well described and that sugar binding induces a pronounced conformational change that may result in local changes in the chemical environment in man ...
MAPK-mediated Phosphorylation of GATA-1 Promotes Expression and Cell Survival* Bcl-X □
... to extracellular stimulation, suppresses the suicidal reaction, and prevents cells from disintegration. We previously demonstrated that GATA-1 is a key regulator of the E4bp4 gene and is also an essential component of the antiapoptotic network of IL-3 in an IL-3-dependent pro-B cell line (17). In th ...
... to extracellular stimulation, suppresses the suicidal reaction, and prevents cells from disintegration. We previously demonstrated that GATA-1 is a key regulator of the E4bp4 gene and is also an essential component of the antiapoptotic network of IL-3 in an IL-3-dependent pro-B cell line (17). In th ...
Distinct Classes of Mitotic Cyclins Are Differentially
... during a short period in S phase, suggesting that it belongs to a nove1 class of plant cyclins. Sequence comparison of 18 plant mitotic cyclins cloned thus far showed that they can be divided into four distinct structural groups with different functions in cell cycle progression. ...
... during a short period in S phase, suggesting that it belongs to a nove1 class of plant cyclins. Sequence comparison of 18 plant mitotic cyclins cloned thus far showed that they can be divided into four distinct structural groups with different functions in cell cycle progression. ...
Two Genes with Similarity to Bacterial Response Regulators Are
... response regulator receiver domain at the C terminus. These proteins have been referred to as hybrid kinases. One exception is ERS (for ethylene response sensor), a member of the Arabidopsis ETR gene family, which is comprised solely of a histidine kinase and a putative ethylene binding domain. Seve ...
... response regulator receiver domain at the C terminus. These proteins have been referred to as hybrid kinases. One exception is ERS (for ethylene response sensor), a member of the Arabidopsis ETR gene family, which is comprised solely of a histidine kinase and a putative ethylene binding domain. Seve ...
Chapter 7: Cellular Pathways That Harvest Chemical Energy
... Energy from Glucose • Pyruvate oxidation and the citric acid cycle produce CO2 and hydrogen atoms carried by NADH and FADH2. • The respiratory chain combines the hydrogens with O2, releasing enough energy for ATP synthesis. Review Figure 7.5a ...
... Energy from Glucose • Pyruvate oxidation and the citric acid cycle produce CO2 and hydrogen atoms carried by NADH and FADH2. • The respiratory chain combines the hydrogens with O2, releasing enough energy for ATP synthesis. Review Figure 7.5a ...
The following Text was taken from the Student Lab Manual for
... For the purpose of studying cell metabolism, as well as for medical treatment and other applications, we can use molecules as enzyme inhibitors to artificially regulate enzyme activity. Some of these inhibitors can bind to enzymes in a reversible or an irreversible fashion. The two primary classes o ...
... For the purpose of studying cell metabolism, as well as for medical treatment and other applications, we can use molecules as enzyme inhibitors to artificially regulate enzyme activity. Some of these inhibitors can bind to enzymes in a reversible or an irreversible fashion. The two primary classes o ...
ADVANCES IN AGRICULTURAL SCIENCES XIV (1–2)
... have broad immunostimulating properties, while their elevated level as a result of stress causes immunosuppressive action (Webster-Marketon and Glaser 2008, Aguilera 2010). Stress is one of the factors activating sympathetic nervous system, as it was evidenced that its activation results in release ...
... have broad immunostimulating properties, while their elevated level as a result of stress causes immunosuppressive action (Webster-Marketon and Glaser 2008, Aguilera 2010). Stress is one of the factors activating sympathetic nervous system, as it was evidenced that its activation results in release ...
Metabolismo dos aminoácidos e proteínas. II. Anabolismo
... Both the GDH and GS-GOGAT pathways produce 1 mole of glutamate from 1 mole each of NH3, 2-oxoglutarate and NADPH. But note that the GS-GOGAT pathway is energetically more costly than the GDH pathway, consuming 1 ATP. Escherichia coli is now known to have two primary pathways for glutamate synthesis. ...
... Both the GDH and GS-GOGAT pathways produce 1 mole of glutamate from 1 mole each of NH3, 2-oxoglutarate and NADPH. But note that the GS-GOGAT pathway is energetically more costly than the GDH pathway, consuming 1 ATP. Escherichia coli is now known to have two primary pathways for glutamate synthesis. ...
Foxp3 drives oxidative phosphorylation and protection
... Foxp3 programs alterations in metabolic flux in Tregs. We first asked whether Foxp3 was sufficient to alter the capacity for OXPHOS and glycolysis in Tregs (10, 13, 14, 19, 20). We compared iTregs with Tact and Foxp3+ (TGFβ Foxp3+) CD4+ T cells from iTreg cultures with Foxp3– (TGFβ Foxp3–) cells fro ...
... Foxp3 programs alterations in metabolic flux in Tregs. We first asked whether Foxp3 was sufficient to alter the capacity for OXPHOS and glycolysis in Tregs (10, 13, 14, 19, 20). We compared iTregs with Tact and Foxp3+ (TGFβ Foxp3+) CD4+ T cells from iTreg cultures with Foxp3– (TGFβ Foxp3–) cells fro ...
Biology: semester one: course outline
... State and describe the two types of cell signaling that are used to pass information through the plasma membrane (without passing chemicals through the membrane). Relating to chemical signaling: o State the three steps of chemical signaling: reception, transduction, response. o Describe the relation ...
... State and describe the two types of cell signaling that are used to pass information through the plasma membrane (without passing chemicals through the membrane). Relating to chemical signaling: o State the three steps of chemical signaling: reception, transduction, response. o Describe the relation ...
Identification of the tRNA-binding Protein Arc1p as a Novel Target of
... both arc1⌬ and arc1⌬los1⌬ mutants, indicating that biotinylation of Arc1p is not essential for activity. ...
... both arc1⌬ and arc1⌬los1⌬ mutants, indicating that biotinylation of Arc1p is not essential for activity. ...
Vaccinia virus protein A46R targets multiple Toll-like
... it has been recognized that Toll-like receptors (TLRs) have a role in innate defense against viruses. Here, we define the function of the vaccinia virus (VV) protein A46R and show it inhibits intracellular signalling by a range of TLRs. TLR signalling is triggered by homotypic interactions between t ...
... it has been recognized that Toll-like receptors (TLRs) have a role in innate defense against viruses. Here, we define the function of the vaccinia virus (VV) protein A46R and show it inhibits intracellular signalling by a range of TLRs. TLR signalling is triggered by homotypic interactions between t ...
Metabolic flux profiling of recombinant protein secreting Pichia
... Several studies have reported on the impact of recombinant protein over expression on different growth parameters of yeast, such as maximum growth rate, biomass yield or substrate specific consumption rate [11–14], suggesting a potential impact on the cell’s central metabolism. Nevertheless, the ...
... Several studies have reported on the impact of recombinant protein over expression on different growth parameters of yeast, such as maximum growth rate, biomass yield or substrate specific consumption rate [11–14], suggesting a potential impact on the cell’s central metabolism. Nevertheless, the ...
Lecture_5_Control_of_glycolysis
... subunit consists of four domains. The biotin carboxylase domain (BC) catalyzes the formation of carboxyphosphate and the subsequent attachment of CO2 to the second domain, the biotin carboxyl carrier protein (BCCP), the site of the covalently attached biotin. Once bound to CO2, BCCP leaves the bioti ...
... subunit consists of four domains. The biotin carboxylase domain (BC) catalyzes the formation of carboxyphosphate and the subsequent attachment of CO2 to the second domain, the biotin carboxyl carrier protein (BCCP), the site of the covalently attached biotin. Once bound to CO2, BCCP leaves the bioti ...
The Ethylene Signal Transduction Pathway in Plants
... ACC oxidase is constitutively present in most tissues, but its synthesis is increased during fruit ripening in tomato. The genes that encode A C C synthase and A C C oxidase have been cloned and characterized from many plant species (5, 6). A C C synthase is encoded by multigene families in all spec ...
... ACC oxidase is constitutively present in most tissues, but its synthesis is increased during fruit ripening in tomato. The genes that encode A C C synthase and A C C oxidase have been cloned and characterized from many plant species (5, 6). A C C synthase is encoded by multigene families in all spec ...
(De)regulation of key enzyme steps in the shikimate pathway and
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
ASPECTS OF ANAEROBIC METABOLISM IN
... participation of glutamate is rather small, and there seems to be no stoichiometric relation to the accumulation of the organic acids. As isotachophoretic analysis did not reveal accumulations of metabolites other than acetate, succinate and propionate, we may conclude that glutamate has been conver ...
... participation of glutamate is rather small, and there seems to be no stoichiometric relation to the accumulation of the organic acids. As isotachophoretic analysis did not reveal accumulations of metabolites other than acetate, succinate and propionate, we may conclude that glutamate has been conver ...
