Essential Cell Biology (3rd ed.)
... nearly all its myriad functions. Enzymes promote intracellular chemical reactions by providing intricate molecular surfaces, contoured with particular bumps and crevices, that can cradle or exclude specific molecules. Proteins embedded in the plasma membrane form the channels and pumps that control ...
... nearly all its myriad functions. Enzymes promote intracellular chemical reactions by providing intricate molecular surfaces, contoured with particular bumps and crevices, that can cradle or exclude specific molecules. Proteins embedded in the plasma membrane form the channels and pumps that control ...
Overview for Electrophoresis and Western Blotting
... glycine mobility is low enough to stack SDS-bound proteins ahead of the glycine zone even when the stacking gel is set up at the same pH as the resolving gel. Proteins as large as 70 kDa will remain stacked in a 4% gel at pH 8.6–8.8 just as well as in a 4% gel at 6.8–7.2. The larger proteins will al ...
... glycine mobility is low enough to stack SDS-bound proteins ahead of the glycine zone even when the stacking gel is set up at the same pH as the resolving gel. Proteins as large as 70 kDa will remain stacked in a 4% gel at pH 8.6–8.8 just as well as in a 4% gel at 6.8–7.2. The larger proteins will al ...
Isolation of protein spots from a silver-stained 2
... automatically. A small piece is precisely excised from the center ...
... automatically. A small piece is precisely excised from the center ...
as a PDF - CiteSeerX
... cultures were treated with EDTA and phenylmethylsulfonyl fluoride to final concentrations of 1 mM each. Samples of 1.0 ml were removed and spun at 15,000 x g for 5 min at 4°C, and the cell pellets were extracted with sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample buffe ...
... cultures were treated with EDTA and phenylmethylsulfonyl fluoride to final concentrations of 1 mM each. Samples of 1.0 ml were removed and spun at 15,000 x g for 5 min at 4°C, and the cell pellets were extracted with sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample buffe ...
Chapter 20. Proteins
... Ionic interactions or Salt Bridges are the attraction of opposite charges for one another. Negatively charged amino acid side groups, such as glutamate and aspartate are attracted to positively charged amino acid side groups( -COO- and -NH3+ side chains) such as lysine, arginine, and histidine. Fina ...
... Ionic interactions or Salt Bridges are the attraction of opposite charges for one another. Negatively charged amino acid side groups, such as glutamate and aspartate are attracted to positively charged amino acid side groups( -COO- and -NH3+ side chains) such as lysine, arginine, and histidine. Fina ...
Proteins
... reaction they catalyze (glutathione transferase (GST) transfers glutathione into the acceptor molecule, whereas glutathione reductase (GR) catalyses reduction of oxidized glutathione into reduced glutathione). The systematic names of enzymes come from the type and the participants of the reaction th ...
... reaction they catalyze (glutathione transferase (GST) transfers glutathione into the acceptor molecule, whereas glutathione reductase (GR) catalyses reduction of oxidized glutathione into reduced glutathione). The systematic names of enzymes come from the type and the participants of the reaction th ...
THE PROTEIN NON-FOLDING PROBLEM: AMINO ACID
... Use of homology provides a means to rapidly increase the amount of disorder data (Table 1) An argument against use of homology is that such sequences are correlated and so adding sequences by this method does not increase the information content very effectively. However, for many proteins with diso ...
... Use of homology provides a means to rapidly increase the amount of disorder data (Table 1) An argument against use of homology is that such sequences are correlated and so adding sequences by this method does not increase the information content very effectively. However, for many proteins with diso ...
Full-Text
... “elongation-phase” protofilaments are formed by further accumulation of prion proteins to the PrPTSE -nuclei. Once larger fibrils are formed they tend to break into smaller units during the “fragmentation phase”. Further cycles of elongation and fragmentation finally result in an exponential increas ...
... “elongation-phase” protofilaments are formed by further accumulation of prion proteins to the PrPTSE -nuclei. Once larger fibrils are formed they tend to break into smaller units during the “fragmentation phase”. Further cycles of elongation and fragmentation finally result in an exponential increas ...
Projection Structure of a Plant Vacuole Membrane Aquaporin by
... a-TIP was routinely obtained. Mature, ungerminated seeds were used so that the puri®ed a-TIP would be at a physiological ground state of phosphorylation (Johnson & Chrispeels, 1992). When obtained from dry seed, a-TIP eluted as a single broad peak by anion-exchange chromatography (Figure 1(c)). By S ...
... a-TIP was routinely obtained. Mature, ungerminated seeds were used so that the puri®ed a-TIP would be at a physiological ground state of phosphorylation (Johnson & Chrispeels, 1992). When obtained from dry seed, a-TIP eluted as a single broad peak by anion-exchange chromatography (Figure 1(c)). By S ...
Engineering and Identifying Supercharged Proteins
... able to adopt large, stable three-dimensional conformations suitable for strong binding to targets even when they lack hydrophobic clefts commonly associated with small-molecule binding. Moreover, the strength of macromolecule-target binding can be sufficient to interfere with native protein–protein ...
... able to adopt large, stable three-dimensional conformations suitable for strong binding to targets even when they lack hydrophobic clefts commonly associated with small-molecule binding. Moreover, the strength of macromolecule-target binding can be sufficient to interfere with native protein–protein ...
doc - ray luo
... Proteins are crucial in transport, catalysis, regulation, and other functions of the human body. Constructed out of one-dimensional strings of amino acids, proteins are transformed into three-dimensional structures in chemically favorable steps in a process known as protein folding (Figure 1). The p ...
... Proteins are crucial in transport, catalysis, regulation, and other functions of the human body. Constructed out of one-dimensional strings of amino acids, proteins are transformed into three-dimensional structures in chemically favorable steps in a process known as protein folding (Figure 1). The p ...
Structural diversity of band 4.1 superfamily members
... of the conserved N-terminal domains of all the band 4.1 superfamily members so far identified. We selected six highly conserved amino acid sequences consisting of 6-8 amino acids, and synthesized the corresponding degenerate oligonucleotides (Fig. 1). PCR and reverse transcriptase-PCR (RT-PCR) were ...
... of the conserved N-terminal domains of all the band 4.1 superfamily members so far identified. We selected six highly conserved amino acid sequences consisting of 6-8 amino acids, and synthesized the corresponding degenerate oligonucleotides (Fig. 1). PCR and reverse transcriptase-PCR (RT-PCR) were ...
Macromolecules - WordPress.com
... Glucose is absorbed into the bloodstream through the intestinal wall. Some of this glucose goes straight to work in our brain cells and red blood cells, while the rest makes its way to the liver and muscles, where it is stored as glycogen (animal starch), and to fat cells, where it is stored as ...
... Glucose is absorbed into the bloodstream through the intestinal wall. Some of this glucose goes straight to work in our brain cells and red blood cells, while the rest makes its way to the liver and muscles, where it is stored as glycogen (animal starch), and to fat cells, where it is stored as ...
The Strategic Use of Ruminally Protected Amino Acids in Dairy
... Many nutritionists have the idea that supplying more AA will result in greater flow to the mammary gland and thus more milk protein. Unfortunately this may not be the case. Once absorbed into the cow, amino acids can contribute to protein, but the majority of the protein is deaminated and used for e ...
... Many nutritionists have the idea that supplying more AA will result in greater flow to the mammary gland and thus more milk protein. Unfortunately this may not be the case. Once absorbed into the cow, amino acids can contribute to protein, but the majority of the protein is deaminated and used for e ...
70-74 Research Article Molecular Docking Studies of Deacetylbisaco
... Docking technique is a method which predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex. Understanding the preferred orientation can be used to predict the strength of binding affinity between two molecules. As such, docking studies can be ...
... Docking technique is a method which predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex. Understanding the preferred orientation can be used to predict the strength of binding affinity between two molecules. As such, docking studies can be ...
Initiation of Innate Immune Responses in the
... The activation of the humoral immune system is contributed by several reactions such as proteolytic cascades leading to blood clotting, melanin formation, opsonization, and a transient synthesis of potent antibacterial peptides (Hoffmann et al., 1999) The clotting system is an important reaction in ...
... The activation of the humoral immune system is contributed by several reactions such as proteolytic cascades leading to blood clotting, melanin formation, opsonization, and a transient synthesis of potent antibacterial peptides (Hoffmann et al., 1999) The clotting system is an important reaction in ...
et al - The EMBO Journal
... wild-type RBR mRNA and protein are present in vegetative cells. In contrast, in meiocytes only a C-terminal truncated RBR protein is present, due to aberrant RBR mRNA splicing in these specialized cells. Accordingly, homozygous rbr-2 mutant plants displayed only some minor vegetative phenotypic aber ...
... wild-type RBR mRNA and protein are present in vegetative cells. In contrast, in meiocytes only a C-terminal truncated RBR protein is present, due to aberrant RBR mRNA splicing in these specialized cells. Accordingly, homozygous rbr-2 mutant plants displayed only some minor vegetative phenotypic aber ...
The Biology of Extracellular Molecular Chaperones. Novartis Foundation
... was later related to the appearance of novel proteins within stressed cells, and the key signal stimulating this appearance was identified as the presence of unfolded proteins within the cell. It is now known that this is a key mechanism enabling cells to survive a multitude of physical, chemical an ...
... was later related to the appearance of novel proteins within stressed cells, and the key signal stimulating this appearance was identified as the presence of unfolded proteins within the cell. It is now known that this is a key mechanism enabling cells to survive a multitude of physical, chemical an ...
C. Flow Chart
... many attempts predict loops, few of them are successful to some extent. These existing methods lack specificity and accuracy, even though the loops are functionally not significant, but their presence brings significant change in overall 3D structure of protein. So accuracy and specificity in loop p ...
... many attempts predict loops, few of them are successful to some extent. These existing methods lack specificity and accuracy, even though the loops are functionally not significant, but their presence brings significant change in overall 3D structure of protein. So accuracy and specificity in loop p ...
MSc in Biochemistry Dissertation Project – 2nd Cycle Student´s
... repertoire of GalNAc-Ts therefore determine which and where proteins are O-glycosylated (Trends in Cell Biology 2011, 21, 149-158). In this context, mucin-type proteins are the main templates catalysed by GalNAc-Ts. In addition, aberrant glycosylation is a universal feature of cancer (Figure 1B) and ...
... repertoire of GalNAc-Ts therefore determine which and where proteins are O-glycosylated (Trends in Cell Biology 2011, 21, 149-158). In this context, mucin-type proteins are the main templates catalysed by GalNAc-Ts. In addition, aberrant glycosylation is a universal feature of cancer (Figure 1B) and ...
Identification of Antigenic Regions of Duck Hepatitis B Virus Core
... (Boehringer Ingelheim, Ingelheim am Rhein, Germany). The test was repeated at least two times for each serum and, between each test, the bound antibodies were removed from the pins according to the manufacturer’s protocol (Chiron) by sonication for 10 min in disruption buffer at 65°C (0.1 M phosphat ...
... (Boehringer Ingelheim, Ingelheim am Rhein, Germany). The test was repeated at least two times for each serum and, between each test, the bound antibodies were removed from the pins according to the manufacturer’s protocol (Chiron) by sonication for 10 min in disruption buffer at 65°C (0.1 M phosphat ...
Table S1.
... Table S1. Functional activity of SOD1 variants. The enzymatic activity of all proteins was compared to the activity of wtSOD1 and a commercial recombinant SOD1 protein (hucSOD1). Of the mutant SOD1 purified, three have mutations located away from the metal binding/catalytic region, thus having prope ...
... Table S1. Functional activity of SOD1 variants. The enzymatic activity of all proteins was compared to the activity of wtSOD1 and a commercial recombinant SOD1 protein (hucSOD1). Of the mutant SOD1 purified, three have mutations located away from the metal binding/catalytic region, thus having prope ...
Dr. John Perozich's Full CV
... Wymore. IX European Symposium of the Protein Society. May 2011. Stockholm, Sweden. Initial analysis of an alignment of 1310 aldehyde dehydrogenases (ALDHs). A few selected families such as succinic semialdehdye dehydrogenases were examined. DNA Gyrase B and HSP90 N-Terminal Domain and Their Role in ...
... Wymore. IX European Symposium of the Protein Society. May 2011. Stockholm, Sweden. Initial analysis of an alignment of 1310 aldehyde dehydrogenases (ALDHs). A few selected families such as succinic semialdehdye dehydrogenases were examined. DNA Gyrase B and HSP90 N-Terminal Domain and Their Role in ...
lecture 7
... - saw GFP fluorescence (green) in the cytosol (i.e., it wasn’t degraded) - has implications for proteins that aggregate in cell and cause diseases ...
... - saw GFP fluorescence (green) in the cytosol (i.e., it wasn’t degraded) - has implications for proteins that aggregate in cell and cause diseases ...
GRA Proteins of Toxoplasma gondii: Maintenance of Host
... tiantigenic vaccine using SAG1 and GRA4 selected on the basis of previous immunological and immunization studies protects well against the infection in mouse [46]. Mortality of susceptible C57BL/6 mice reduced upon oral challenge with cysts of the 76K type II strain by 62% survival and the protectio ...
... tiantigenic vaccine using SAG1 and GRA4 selected on the basis of previous immunological and immunization studies protects well against the infection in mouse [46]. Mortality of susceptible C57BL/6 mice reduced upon oral challenge with cysts of the 76K type II strain by 62% survival and the protectio ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.