Does a backwardly read protein sequence have a unique native state?
... a stable three-helix bundle motif with helix I (Gin 10 to His 19) tilted with respect to the antiparallel hairpin formed by helices II (Glu26 to Asp37) and m (Ser42-Ala55). The N-terminal residues up to Glu9 and the C-terminal residues from Gln56 to the terminal lysine do not exhibit ordered structu ...
... a stable three-helix bundle motif with helix I (Gin 10 to His 19) tilted with respect to the antiparallel hairpin formed by helices II (Glu26 to Asp37) and m (Ser42-Ala55). The N-terminal residues up to Glu9 and the C-terminal residues from Gln56 to the terminal lysine do not exhibit ordered structu ...
FYVE-Dependent Endosomal Targeting of an Arrestin
... In this report, we chose to focus on the protein AdcA from D. discoideum (Figure 1A–B). Arrestin-domain containing proteins extended with a FYVE domain were also found in D. purpureum, Polysphondylium pallidum, Entamoeba histolytica and E. dispar, other members of the Conosea group within the Amoebo ...
... In this report, we chose to focus on the protein AdcA from D. discoideum (Figure 1A–B). Arrestin-domain containing proteins extended with a FYVE domain were also found in D. purpureum, Polysphondylium pallidum, Entamoeba histolytica and E. dispar, other members of the Conosea group within the Amoebo ...
Bioinformatics in Brief This week: DB for structures Structure
... Homologous Superfamily, HThis level groups together protein domains which are thought to share a common ancestor and can therefore be described as homologous. Similarities are identified first by sequence comparisons and subsequently by structure comparison using SSAP. the criteria: Sequence identit ...
... Homologous Superfamily, HThis level groups together protein domains which are thought to share a common ancestor and can therefore be described as homologous. Similarities are identified first by sequence comparisons and subsequently by structure comparison using SSAP. the criteria: Sequence identit ...
Gene Section STK4 (serine/threonine kinase 4) Atlas of Genetics and Cytogenetics
... STK4 plays a role in promoting apotosis, in particular, in chromosome condensation during programmed cell death. STK4 is cleaved by caspase 3 during apoptosis, releasing the highly active N-terminal kinase domain. This active protein promotes apoptosis by activating JNK and also by further caspase a ...
... STK4 plays a role in promoting apotosis, in particular, in chromosome condensation during programmed cell death. STK4 is cleaved by caspase 3 during apoptosis, releasing the highly active N-terminal kinase domain. This active protein promotes apoptosis by activating JNK and also by further caspase a ...
Function of Conserved Tryptophans in the Aspergillus niger
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmic folding of proteins has not been studied in any great det ...
... only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmic folding of proteins has not been studied in any great det ...
PPT - Bruce Blumberg
... • affinity purify antibody with phage fusion protein – western with original protein – advantages • best choice if only antibody is available – disadvantages • λgt11 and relatives are painful to work with • your antibody may not be suitable – sugar directed – structural epitope BioSci 203 blumberg l ...
... • affinity purify antibody with phage fusion protein – western with original protein – advantages • best choice if only antibody is available – disadvantages • λgt11 and relatives are painful to work with • your antibody may not be suitable – sugar directed – structural epitope BioSci 203 blumberg l ...
MONOCLONAL ANTIBODY TO HUMAN C3/C3a/C3a
... N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot Application IA has been tested by Hycult Biotech. ...
... N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot Application IA has been tested by Hycult Biotech. ...
Amino Acid Composition of Enzymatically Hydrolysed Potato Protein
... Poland). All chemicals used were of analytical grade. Preparation of potato protein isolates from potato juice. Potato protein isolates (PPI) were obtained by thermal coagulation in the presence or in the absence of such salts like calcium chloride and calcium lactate. Their concentrations were ca 0 ...
... Poland). All chemicals used were of analytical grade. Preparation of potato protein isolates from potato juice. Potato protein isolates (PPI) were obtained by thermal coagulation in the presence or in the absence of such salts like calcium chloride and calcium lactate. Their concentrations were ca 0 ...
domain alignments - Oxford Academic
... proteins in Entrez. CDART, which stores that information, can be invoked from within Entrez to visualize domain architectures (6). Proteins in Entrez can now be neighboured by similar domain architecture, in addition to sequence similarity as detected by BLAST (7). Conserved Domains are neighboured ...
... proteins in Entrez. CDART, which stores that information, can be invoked from within Entrez to visualize domain architectures (6). Proteins in Entrez can now be neighboured by similar domain architecture, in addition to sequence similarity as detected by BLAST (7). Conserved Domains are neighboured ...
Optimization of Programmed Suppression in a Cell
... in a reaction mixture may influence not only the suppression yield but also the extent of background suppression (misreading by the malfunction of ribosome, which is the adaptation of noncognate tRNA as a decoder). Figure 2 exhibits the effects of different Mg2+ concentrations on EPO mutein synthesi ...
... in a reaction mixture may influence not only the suppression yield but also the extent of background suppression (misreading by the malfunction of ribosome, which is the adaptation of noncognate tRNA as a decoder). Figure 2 exhibits the effects of different Mg2+ concentrations on EPO mutein synthesi ...
212_spring_2005_oxygen transport
... Allosteric proteins are typically multisubunit proteins Small molecules know as allosteric effectors bind to the protein at sites that are spatially distinct from the ligand binding site and exert either a positive or negative effect on ligand binding ...
... Allosteric proteins are typically multisubunit proteins Small molecules know as allosteric effectors bind to the protein at sites that are spatially distinct from the ligand binding site and exert either a positive or negative effect on ligand binding ...
The protein import apparatus of chloroplasts
... (precursor form of the small subunit of ribulose-1.5bisphosphate carboxylase-oxygenase. Rubisco) was without influence on the translocation efficiency. These data indicate that a tightly folded polypeptide which is "arrested" in its conformation can not be converted by the translocation machinery in ...
... (precursor form of the small subunit of ribulose-1.5bisphosphate carboxylase-oxygenase. Rubisco) was without influence on the translocation efficiency. These data indicate that a tightly folded polypeptide which is "arrested" in its conformation can not be converted by the translocation machinery in ...
Granny Guide
... significantly as they get older, as the body only requires a very small amount of phenylalanine to function properly. ...
... significantly as they get older, as the body only requires a very small amount of phenylalanine to function properly. ...
Supplemental Text
... GSSG levels similar to or even higher than APAP alone. This indicated that the delayed GSH treatment did not prevent the mitochondrial oxidant stress, which is presumably initiated by binding of NAPQI to mitochondrial proteins.15 These data provided strong evidence that the delayed treatment with G ...
... GSSG levels similar to or even higher than APAP alone. This indicated that the delayed GSH treatment did not prevent the mitochondrial oxidant stress, which is presumably initiated by binding of NAPQI to mitochondrial proteins.15 These data provided strong evidence that the delayed treatment with G ...
Mass Spectrometry-Based Analysis Of Membrane Proteins Derived
... proteins not yet reported to be involved in CLL or MM have been found with this novel approach, e.g. Proline-, glutamic acid- and leucine-rich protein 1 or N-acetyltransferase 10. This underlines the importance of density gradient ultracentrifugation or other membrane enrichment strategies when anal ...
... proteins not yet reported to be involved in CLL or MM have been found with this novel approach, e.g. Proline-, glutamic acid- and leucine-rich protein 1 or N-acetyltransferase 10. This underlines the importance of density gradient ultracentrifugation or other membrane enrichment strategies when anal ...
Troubleshooting SDS-PAGE-0410
... A more rapid polymerization can be accomplished by degassing the acrylamide solution. TEMED and ammonium persulfate are left out of the gel mixture. Increase ammonium persulfate or TEMED. Use fresh ammonium persulfate and new TEMED. The temperature is too low, cast at room temperature. Quality of th ...
... A more rapid polymerization can be accomplished by degassing the acrylamide solution. TEMED and ammonium persulfate are left out of the gel mixture. Increase ammonium persulfate or TEMED. Use fresh ammonium persulfate and new TEMED. The temperature is too low, cast at room temperature. Quality of th ...
Poster for RCPSC mee.. - University of Alberta
... (GlcCer) is a metabolite of ceramide produced by the glycosylation of the 1-hydroxyl group of ceramide by the enzyme Glucosylceramide Synthase (GCS) (Figure 1). Given the similarities in structure between the natural product inhibitors of PP1, the clavosines, and the sphingolipid GlcCer, we hypothes ...
... (GlcCer) is a metabolite of ceramide produced by the glycosylation of the 1-hydroxyl group of ceramide by the enzyme Glucosylceramide Synthase (GCS) (Figure 1). Given the similarities in structure between the natural product inhibitors of PP1, the clavosines, and the sphingolipid GlcCer, we hypothes ...
Trends in Plant Science
... such as the large-scale generation of T-DNA insertional mutant lines have been initiated [2], complementary approaches will probably be needed until the function of every gene product of Arabidopsis and other model plants is known. Interestingly, in addition to the holistic or ‘top-down’ approach th ...
... such as the large-scale generation of T-DNA insertional mutant lines have been initiated [2], complementary approaches will probably be needed until the function of every gene product of Arabidopsis and other model plants is known. Interestingly, in addition to the holistic or ‘top-down’ approach th ...
Amino acid sequence of an immunogenic corneal stromal protein.
... CO-Ag and the S-100 proteins ranges from 25% (S100L) to 81% (calgraulin C). The S-100 are a group of low molecular weight (approximately 10 kDa) acidic Ca2+-binding proteins. These proteins are expressed in a cell lineage-specific or tissue-specific manner." To date, at least 14 proteins of the S-10 ...
... CO-Ag and the S-100 proteins ranges from 25% (S100L) to 81% (calgraulin C). The S-100 are a group of low molecular weight (approximately 10 kDa) acidic Ca2+-binding proteins. These proteins are expressed in a cell lineage-specific or tissue-specific manner." To date, at least 14 proteins of the S-10 ...
Homology between the DNA-binding domain of the GCN4
... thought to be mediated through a helix-turn-helix structure (13-16). Schemes for the prediction of secondary structure also reveal a strong preference for a-helical conformation in the carboxyl-terminal region of jun, which is homologous to the GCN4 DNA-binding domain. We suggest that the jun protei ...
... thought to be mediated through a helix-turn-helix structure (13-16). Schemes for the prediction of secondary structure also reveal a strong preference for a-helical conformation in the carboxyl-terminal region of jun, which is homologous to the GCN4 DNA-binding domain. We suggest that the jun protei ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.