BIO 402 - National Open University of Nigeria
... In 1928 and 1929 Heitz was the first to distinguish two types of chromatin, which he named euchromatin and heterochromatin. Euchromatin stains lightly or not at all in interphase and prophase, while heterochromatin stains darkly in these stages. Heterochromatin is an extremely helpful marker for chr ...
... In 1928 and 1929 Heitz was the first to distinguish two types of chromatin, which he named euchromatin and heterochromatin. Euchromatin stains lightly or not at all in interphase and prophase, while heterochromatin stains darkly in these stages. Heterochromatin is an extremely helpful marker for chr ...
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
... Background: Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni is scattered into several lineages, with no apparent linkage, whereas C. coli clusters into three distinct phylogenetic ...
... Background: Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni is scattered into several lineages, with no apparent linkage, whereas C. coli clusters into three distinct phylogenetic ...
Myr 8, A Novel Unconventional Myosin Expressed during Brain
... 4 are described above. Probe 5 represents nucleotides 4110 – 4771, which correspond to the sequence within the tail domain that is unique to the myr 8b isoform. PCR amplification of myr 8 isoforms. PCR amplification of cerebellar granule neuron cDNA was performed using Taq polymerase with a common s ...
... 4 are described above. Probe 5 represents nucleotides 4110 – 4771, which correspond to the sequence within the tail domain that is unique to the myr 8b isoform. PCR amplification of myr 8 isoforms. PCR amplification of cerebellar granule neuron cDNA was performed using Taq polymerase with a common s ...
Stamina pistilloida, the Pea Ortholog of Fim and
... Secondary flowers also often possessed their own ectopic (tertiary) flowers (not shown). By this stage, the whorled arrangement of organs was completely abandoned. Outer organs of tertiary flowers were generally sepaloid; inner organs were variable and could be sepal-, carpel-, petal-, or stamen-lik ...
... Secondary flowers also often possessed their own ectopic (tertiary) flowers (not shown). By this stage, the whorled arrangement of organs was completely abandoned. Outer organs of tertiary flowers were generally sepaloid; inner organs were variable and could be sepal-, carpel-, petal-, or stamen-lik ...
The Major Component of the Paraflagellar Rod of Trypanosoma
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
The role of the C8 proton of ATP in the catalysis of shikimate kinase
... making up the “push” mechanism (Figure 1) [2]. Shown are the protein backbone carbonyl associated with the C6-NH2, the Thr associated with the proton transfer from C8-H to the α-PO4 (SK, Thr17; AK1, Thr23), the Arg associated with C8 protonation (SK, Arg110: AK1; Arg128), the Arg coordinated to the ...
... making up the “push” mechanism (Figure 1) [2]. Shown are the protein backbone carbonyl associated with the C6-NH2, the Thr associated with the proton transfer from C8-H to the α-PO4 (SK, Thr17; AK1, Thr23), the Arg associated with C8 protonation (SK, Arg110: AK1; Arg128), the Arg coordinated to the ...
Recycling of vitamin B12 and NAD+ within the Pdu
... Purified PduQ enzyme was identified as an oxygen-sensitive iron-dependent alcohol dehydrogenase (ADH) that catalyzes the interconversion between propionaldehyde and 1-propanol in vitro. The propionaldehyde reduction activity of PduQ was about 45 times higher than that of the 1-propanol oxidation at ...
... Purified PduQ enzyme was identified as an oxygen-sensitive iron-dependent alcohol dehydrogenase (ADH) that catalyzes the interconversion between propionaldehyde and 1-propanol in vitro. The propionaldehyde reduction activity of PduQ was about 45 times higher than that of the 1-propanol oxidation at ...
Quality control in tRNA charging — editing of homocysteine
... Met-tRNAs increase up to 10-fold under stress conditions, such as oxidative stress, exposure to toll-like receptor ligands, or viral infection, and the Met-misacylated tRNAs are used in protein synthesis. Although the data appear to be convincing, such extraordinarily high tRNA misacylation rates ar ...
... Met-tRNAs increase up to 10-fold under stress conditions, such as oxidative stress, exposure to toll-like receptor ligands, or viral infection, and the Met-misacylated tRNAs are used in protein synthesis. Although the data appear to be convincing, such extraordinarily high tRNA misacylation rates ar ...
Archive Microbiology
... was used for the assays of lipoic acid. In the beginning it was grown in exactly the same medium as described by Gunsalus a n d Razzell (1957). However, even after repeated passage into fresh medium, these cultures did not respond significantly to the addition of lipoic acid. Since vitamin free case ...
... was used for the assays of lipoic acid. In the beginning it was grown in exactly the same medium as described by Gunsalus a n d Razzell (1957). However, even after repeated passage into fresh medium, these cultures did not respond significantly to the addition of lipoic acid. Since vitamin free case ...
Pseudouridine at position 55 in tRNA controls the contents of other
... chaperone activity of TruB, which was previously hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these m ...
... chaperone activity of TruB, which was previously hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these m ...
An organism containing a normal chromosome complement and
... Other aneuploids (i.e. primary trisomics, tetrasomics, multiple trisomics, secondary trisomics, tertiary trisomics, compensating trisomics) ...
... Other aneuploids (i.e. primary trisomics, tetrasomics, multiple trisomics, secondary trisomics, tertiary trisomics, compensating trisomics) ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... antibody, mitochondrial protein from yeast expressing wild-type rat CrAT (Section 3.1.) was separated by SDS-PAGE and subjected to immunoblotting using different dilutions of the anti-rat CrAT antibody, ranging from 1:3,000 to 1:10,000. Goat anti-rabbit secondary antibody was applied at a 1:6,000 di ...
... antibody, mitochondrial protein from yeast expressing wild-type rat CrAT (Section 3.1.) was separated by SDS-PAGE and subjected to immunoblotting using different dilutions of the anti-rat CrAT antibody, ranging from 1:3,000 to 1:10,000. Goat anti-rabbit secondary antibody was applied at a 1:6,000 di ...
Selecting Degenerate Multiplex PCR Primers
... primers ACACA, ACACC, ACCCA, ACCCC . The number of primers that a degenerate primer represents is referred to as its degeneracy. Degenerate primers are as easy to produce as regular primers, and therefore save the molecular biologist time during the primer design phase of the experiment. The use o ...
... primers ACACA, ACACC, ACCCA, ACCCC . The number of primers that a degenerate primer represents is referred to as its degeneracy. Degenerate primers are as easy to produce as regular primers, and therefore save the molecular biologist time during the primer design phase of the experiment. The use o ...
Perspectives in Diabetes Glucokinase Gene Structure
... isoform generated in the liver ( L l ) differs from those gen- generate the glucokinase activity measured in insulin-proerated in insulin-producing cells (B1 and 82) by sequences ducing cells. It would be surprising if activity of the B1 isoform at the NH,-terminal. This difference accounts for no m ...
... isoform generated in the liver ( L l ) differs from those gen- generate the glucokinase activity measured in insulin-proerated in insulin-producing cells (B1 and 82) by sequences ducing cells. It would be surprising if activity of the B1 isoform at the NH,-terminal. This difference accounts for no m ...
Distribution and structural variation of the she pathogenicity island in
... coli (EHEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), Yersinia enterocolitica and Salmonella enterica serovar Typhimurium. The study showed that the she PAI has undergone a variety of structural changes, de®ned by the presence or absence of speci®c marker genes in the PAI. The ...
... coli (EHEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), Yersinia enterocolitica and Salmonella enterica serovar Typhimurium. The study showed that the she PAI has undergone a variety of structural changes, de®ned by the presence or absence of speci®c marker genes in the PAI. The ...
NAD+-dependent formate dehydrogenase. From a model enzyme to
... Available structures Several high resolution structures of PseFDH are available to date: the apo-enzyme (resolution 1.80 Å) [17], the ternary complex of enzyme with NAD+ and azide mimicking putative transition state (2.05 Å) [17], and a complex with ADPR (1.50 Å) [23]. Several other binary complexes ...
... Available structures Several high resolution structures of PseFDH are available to date: the apo-enzyme (resolution 1.80 Å) [17], the ternary complex of enzyme with NAD+ and azide mimicking putative transition state (2.05 Å) [17], and a complex with ADPR (1.50 Å) [23]. Several other binary complexes ...
Loss of heterozygosity at D8S262: an early genetic event of
... cancer progression. Single-nucleotide polymorphism (SNP) arrays have been used to detect genome-wide abnormalities, such as copy number changes that include loss of heterozygosity (LOH), deletions, and gene amplification events in various types of cancer, and localization of the regions of many onco ...
... cancer progression. Single-nucleotide polymorphism (SNP) arrays have been used to detect genome-wide abnormalities, such as copy number changes that include loss of heterozygosity (LOH), deletions, and gene amplification events in various types of cancer, and localization of the regions of many onco ...
Environmental Microbiology
... consistent with previous findings: Dosselaere (2000) reported expression studies of an ipdC–gusA promoter fusion (Vande Broek et al., 1999), which also contained the whole promoter region of Sp245. They examined changes in expression level due to growth on MMAB medium supplemented with the amino aci ...
... consistent with previous findings: Dosselaere (2000) reported expression studies of an ipdC–gusA promoter fusion (Vande Broek et al., 1999), which also contained the whole promoter region of Sp245. They examined changes in expression level due to growth on MMAB medium supplemented with the amino aci ...
Fitness landscapes of gene regulation in variable - UvA-DARE
... with the optimal Pgal concentrations as obtained directly from the growth data in figure 6.1. Although the optimal level shows a very sharp Pgal dependence, this does not mean that the growth difference for optimal and non-optimal expression are necessarily large. We can see from the landscape in fig. ...
... with the optimal Pgal concentrations as obtained directly from the growth data in figure 6.1. Although the optimal level shows a very sharp Pgal dependence, this does not mean that the growth difference for optimal and non-optimal expression are necessarily large. We can see from the landscape in fig. ...
Biosynthesis of Lipids and Hydrocarbons in Algae
... the process of binding of CoA (Joyard et al., 2010). Oppositely, fatty acid is necessary to be activated by the binding of ACP for passing through the cell membrane in the cyanobacteria Synechocystis species. In any step, a synthesized carbon chain can be metabolized into various products including ...
... the process of binding of CoA (Joyard et al., 2010). Oppositely, fatty acid is necessary to be activated by the binding of ACP for passing through the cell membrane in the cyanobacteria Synechocystis species. In any step, a synthesized carbon chain can be metabolized into various products including ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.