Proteomic sensitivity to dietary manipulations in rainbow trout
... fish. A proteomics approach was used to study the protein profiles of livers of rainbow trout that have been fed two diets containing different proportions of plant ingredients. Both diets control (C) and soy (S) contained fish meal and plant ingredients and synthetic amino acids, but diet S had a g ...
... fish. A proteomics approach was used to study the protein profiles of livers of rainbow trout that have been fed two diets containing different proportions of plant ingredients. Both diets control (C) and soy (S) contained fish meal and plant ingredients and synthetic amino acids, but diet S had a g ...
Structural and Thermal Stability Characterization of Protein
... 1.4.1. Agarose and SDS-PAGE Gel electrophoresis Agarose gel electrophoresis was run on the samples of protein in varying concentration of capric acid before and after the steam sterilization done. It was observed that the hemoglobin, catalase and lysozyme became more negative after steam sterilizati ...
... 1.4.1. Agarose and SDS-PAGE Gel electrophoresis Agarose gel electrophoresis was run on the samples of protein in varying concentration of capric acid before and after the steam sterilization done. It was observed that the hemoglobin, catalase and lysozyme became more negative after steam sterilizati ...
Calculating Nucleic Acid or Protein Concentration
... calculated for common microplates (Table 4). Because pathlength values are proportional to the volume of liquid used, a linear regression has been calculated and can be used to determine the pathlength of any volume between 50 and 250 μl where x = volume used and y = pathlength. Once the pathlength ...
... calculated for common microplates (Table 4). Because pathlength values are proportional to the volume of liquid used, a linear regression has been calculated and can be used to determine the pathlength of any volume between 50 and 250 μl where x = volume used and y = pathlength. Once the pathlength ...
Analysis of DNA transcription termination sequences of gene coding
... dissociation of TEC into RNA, DNA and an RNA polymerase, what means the ending of transcription. In this study, the sequences located between phaC1 and phaZ genes in Pseudomonas species were analysed to identify the bacteria for Rho-independent transcription terminator. Five of studied sequences (tw ...
... dissociation of TEC into RNA, DNA and an RNA polymerase, what means the ending of transcription. In this study, the sequences located between phaC1 and phaZ genes in Pseudomonas species were analysed to identify the bacteria for Rho-independent transcription terminator. Five of studied sequences (tw ...
NSC 108 - National Open University of Nigeria
... utilization of fuel molecules such as glucose. By studying and understanding these highly complex reactions, medical biochemists have found better ways to fight infections and diseases at the molecular level. Since an Engineer cannot repair a vehicle if he does not understand how it works, so a Nurs ...
... utilization of fuel molecules such as glucose. By studying and understanding these highly complex reactions, medical biochemists have found better ways to fight infections and diseases at the molecular level. Since an Engineer cannot repair a vehicle if he does not understand how it works, so a Nurs ...
Supplementary document Trehalose/2
... method (Falicia Goh et al 2011). Cell pellets were washed three times in a sterile isotonic ...
... method (Falicia Goh et al 2011). Cell pellets were washed three times in a sterile isotonic ...
Identification and removal of colanic acid from plasmid DNA
... used as a standard by the Food and Drug Administration to validate plasmid DNA purity for use in patients. However, HPLC cannot detect these contaminating polysaccharides. The MWs of the residual polysaccharides vary over too wide a range. Therefore, not enough polysaccharide at any given MW is pres ...
... used as a standard by the Food and Drug Administration to validate plasmid DNA purity for use in patients. However, HPLC cannot detect these contaminating polysaccharides. The MWs of the residual polysaccharides vary over too wide a range. Therefore, not enough polysaccharide at any given MW is pres ...
Engineering increased vitamin C levels in plants by overexpression
... A. thaliana lines ectopically expressing GalUR showed increased levAF039182) are a codeinone reductase from the opium poppy els of ascorbic acid. The ascorbic acid content of three lines (3, 10, (Papaver somniferum)16 and chalcone reductases from soybean and 17) was two to three times higher than th ...
... A. thaliana lines ectopically expressing GalUR showed increased levAF039182) are a codeinone reductase from the opium poppy els of ascorbic acid. The ascorbic acid content of three lines (3, 10, (Papaver somniferum)16 and chalcone reductases from soybean and 17) was two to three times higher than th ...
Two groups of human herpesvirus 6 identified by sequence
... (Martin et al., 1991; Lindquester & Pellett, 1991). In this study we investigated the molecular basis for differences between HH¥-6 strain groups by PCR and sequence analysis of 15 variants, including laboratory reference strains from both groups. We also studied, by PCR, strains in two potential pa ...
... (Martin et al., 1991; Lindquester & Pellett, 1991). In this study we investigated the molecular basis for differences between HH¥-6 strain groups by PCR and sequence analysis of 15 variants, including laboratory reference strains from both groups. We also studied, by PCR, strains in two potential pa ...
Structure–function relationships of the variable - IMGT
... module (association of the VH and VL, here V-KAPPA, domains). In constrast to schematic, symmetric and fixed Y-shaped representations usually presented in textbooks, this crystal structure illustrates the asymmetry of the antibody, which results from the high degree of freedom of its three arms arou ...
... module (association of the VH and VL, here V-KAPPA, domains). In constrast to schematic, symmetric and fixed Y-shaped representations usually presented in textbooks, this crystal structure illustrates the asymmetry of the antibody, which results from the high degree of freedom of its three arms arou ...
ppt - Vanderbilt University
... 27.8: Introduction to Peptide Structure Determination. Protein Structure: primary (1°) structure: the amino acid sequence secondary (2°): frequently occurring substructures or folds tertiary (3°): three-dimensional arrangement of all atoms in a single polypeptide chain quaternary (4°): overall orga ...
... 27.8: Introduction to Peptide Structure Determination. Protein Structure: primary (1°) structure: the amino acid sequence secondary (2°): frequently occurring substructures or folds tertiary (3°): three-dimensional arrangement of all atoms in a single polypeptide chain quaternary (4°): overall orga ...
Rare genomic changes and mitochondrial sequences
... have also proven to be phylogenetically informative within some groups, such as ticks (Murrell et al., 2003) and spiders (Masta and Boore, 2008). Besides potentially providing new types of genome structure characters for making phylogenetic inferences, mitochondrial genomes provide a rich source of ...
... have also proven to be phylogenetically informative within some groups, such as ticks (Murrell et al., 2003) and spiders (Masta and Boore, 2008). Besides potentially providing new types of genome structure characters for making phylogenetic inferences, mitochondrial genomes provide a rich source of ...
Ypr140wp, `the yeast tafazzin`, displays a mitochondrial
... phase) or ∼ 15–17 (stationary phase). The resulting pellets were then washed twice with 40 ml of water and resuspended in 2 ml of water. To extract yeast lipids from whole cells, 500 µl of the cell suspensions were vigorously shaken for 10 min with glass beads in the presence of 2 ml of chloroform/m ...
... phase) or ∼ 15–17 (stationary phase). The resulting pellets were then washed twice with 40 ml of water and resuspended in 2 ml of water. To extract yeast lipids from whole cells, 500 µl of the cell suspensions were vigorously shaken for 10 min with glass beads in the presence of 2 ml of chloroform/m ...
Fragile x syndrome
... • Mutations in the FMR1 gene cause fragile X syndrome. The FMR1 gene provides instructions for making a protein called fragile X mental retardation 1 protein, or FMRP. ...
... • Mutations in the FMR1 gene cause fragile X syndrome. The FMR1 gene provides instructions for making a protein called fragile X mental retardation 1 protein, or FMRP. ...
a hint of the same genetic defect as in Fechtner syndrome
... Our work sheds more light on the genetics of giant platelet syndromes that are actually a part of the Alport-like syndromes. An updated comparison between the Alport-like families—the X-linked and recessive forms of Alport syndrome—is illustrated in Table 2. The fact that all giant platelet syndrome ...
... Our work sheds more light on the genetics of giant platelet syndromes that are actually a part of the Alport-like syndromes. An updated comparison between the Alport-like families—the X-linked and recessive forms of Alport syndrome—is illustrated in Table 2. The fact that all giant platelet syndrome ...
Obtaining genetic testing in pediatric epilepsy
... NGS technology as applied to WES. Triplet repeats, as in fragile X, abnormal methylation, as in Angelman syndrome, and some large insertions, deletions, and duplications can be missed by WES. In general turn-around time for NGS is also much longer (up to 4 months) than for single gene sequencing due ...
... NGS technology as applied to WES. Triplet repeats, as in fragile X, abnormal methylation, as in Angelman syndrome, and some large insertions, deletions, and duplications can be missed by WES. In general turn-around time for NGS is also much longer (up to 4 months) than for single gene sequencing due ...
Determination and changes of free amino acids in royal
... 3.2. Gas chromatography (GC) and mass fragmentation of the derivatized free amino acids Although performing ion exchange chromatography, the eluate was rich in short chain free fatty acids, which represented 80% of the lipid fraction of RJ. The efficiency of ion exchange chromatography was evaluated ...
... 3.2. Gas chromatography (GC) and mass fragmentation of the derivatized free amino acids Although performing ion exchange chromatography, the eluate was rich in short chain free fatty acids, which represented 80% of the lipid fraction of RJ. The efficiency of ion exchange chromatography was evaluated ...
Molecular Characterization of a Hamster Oviduct
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
Molecular Evolution of the CMT1A-REP Region: A Human
... The CMT1A-REP repeat consists of two copies of a 24-kb sequence on human chromosome 17p11.2–12 that flank a 1.5-Mb region containing a dosage-sensitive gene, peripheral nerve protein-22 (PMP22). Unequal meiotic crossover mediated by misalignment of proximal and distal copies of the CMT1A-REP in huma ...
... The CMT1A-REP repeat consists of two copies of a 24-kb sequence on human chromosome 17p11.2–12 that flank a 1.5-Mb region containing a dosage-sensitive gene, peripheral nerve protein-22 (PMP22). Unequal meiotic crossover mediated by misalignment of proximal and distal copies of the CMT1A-REP in huma ...
Projection Structure of a Plant Vacuole Membrane Aquaporin by
... ®tness and survival (Sanhewe & Ellis, 1996a,b). Immediately after germination, a-TIP may regulate the rehydration of PSVs, osmoregulate the cytoplasm during nutrient export from the PSVs, or adjust vacuolar volume as the central vacuole reforms (reviewed by Maurel et al., 1997). The major intrinsic ...
... ®tness and survival (Sanhewe & Ellis, 1996a,b). Immediately after germination, a-TIP may regulate the rehydration of PSVs, osmoregulate the cytoplasm during nutrient export from the PSVs, or adjust vacuolar volume as the central vacuole reforms (reviewed by Maurel et al., 1997). The major intrinsic ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... locations, the requirement of a TTTV PAM may limit the availability of suitable target sites, reducing the practical utility of Cpf1. To address this limitation, we aimed to engineer variants of Cpf1 that can recognize alternative PAM sequences in order to increase its targeting range. Previous work ...
... locations, the requirement of a TTTV PAM may limit the availability of suitable target sites, reducing the practical utility of Cpf1. To address this limitation, we aimed to engineer variants of Cpf1 that can recognize alternative PAM sequences in order to increase its targeting range. Previous work ...
... The Atlas of Genetics and Cytogenetics in Oncology and Haematology is a peer reviewed on-line journal in open access, devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases. It presents structured review articles (“cards”) on genes, leukaemias, solid tumours, canc ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.