Facts about the mini-Tn7 transposon system as a tool for
... sequences located on the delivery plasmid between the transposon ends, Tn7L and Tn7R (see Fig. 2). The delivery plasmid is some times also named the carrier plasmid. Delivery plasmids The delivery plasmids presented here are all pUC19 derivatives, which can replicate in E. coli and other Enterics, b ...
... sequences located on the delivery plasmid between the transposon ends, Tn7L and Tn7R (see Fig. 2). The delivery plasmid is some times also named the carrier plasmid. Delivery plasmids The delivery plasmids presented here are all pUC19 derivatives, which can replicate in E. coli and other Enterics, b ...
SEARCH_16S: A new algorithm for identifying 16S
... peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
... peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
Two Pedigrees of Familial Advanced Sleep Phase Syndrome in Japan
... time (which could be fictitiously modified) generally varied more than did the wake time (which depends more on the circadian system) in the affected subjects, resulting in sleeping less on weekdays than on weekends. As Jones suggested,6 we also observed that affected members tended to fall asleep a ...
... time (which could be fictitiously modified) generally varied more than did the wake time (which depends more on the circadian system) in the affected subjects, resulting in sleeping less on weekdays than on weekends. As Jones suggested,6 we also observed that affected members tended to fall asleep a ...
Ecological and molecular investigations of cyanotoxin production
... from observations of environmental e¡ects on toxin production. As most research in this ¢eld has focused on microcystin the discussion of putative intracellular functions is limited to this toxin. Toxin production as a mechanism for shunting excess metabolites during periods of environmental stress ...
... from observations of environmental e¡ects on toxin production. As most research in this ¢eld has focused on microcystin the discussion of putative intracellular functions is limited to this toxin. Toxin production as a mechanism for shunting excess metabolites during periods of environmental stress ...
The importance of alternative splicing in the drug discovery process
... common to both protein isoforms are beige; isoform-specific regions are red and green.The black also important when designing knockantibody binds the common region, and will therefore bind both variants.The blue and yellow antibodies are isoform-specific. out mice experiments. For example, the mouse ...
... common to both protein isoforms are beige; isoform-specific regions are red and green.The black also important when designing knockantibody binds the common region, and will therefore bind both variants.The blue and yellow antibodies are isoform-specific. out mice experiments. For example, the mouse ...
Genetic or epigenetic difference causing discordance between
... identify endophenotypes associated with the disease. The former strategy was used, for example, to identify the role of birth complications in the etiology of schizophrenia.1,2 Using the latter approach, decreased hippocampal volume in schizophrenia3 was established as an intermediate phenotype.4 Th ...
... identify endophenotypes associated with the disease. The former strategy was used, for example, to identify the role of birth complications in the etiology of schizophrenia.1,2 Using the latter approach, decreased hippocampal volume in schizophrenia3 was established as an intermediate phenotype.4 Th ...
Rapid generation of nested chromosomal
... targeting vectors have to be built, and three transfection steps are required. Also germ-line transmission was tested for all intermediates. The efficiency of this approach has been improved by the development of libraries of premade insertion targeting vectors and the repair of a point mutation in ...
... targeting vectors have to be built, and three transfection steps are required. Also germ-line transmission was tested for all intermediates. The efficiency of this approach has been improved by the development of libraries of premade insertion targeting vectors and the repair of a point mutation in ...
Y chromosome polymorphisms in medicine
... represents a unique peculiarity of males. Since its ®rst description in 1921 (1), the scienti®c community has been faced with divergent opinions about its role and importance in humans. Due to the abundance of tandemly repeated satellite DNA and the apparent paucity of gene content, the Y chromosome ...
... represents a unique peculiarity of males. Since its ®rst description in 1921 (1), the scienti®c community has been faced with divergent opinions about its role and importance in humans. Due to the abundance of tandemly repeated satellite DNA and the apparent paucity of gene content, the Y chromosome ...
Selection for TnlO Tet Repressor Binding to tet Operator
... is represented by a linear bar with both the N- and C-terminal ends indicated. The solid portion defines the potential a-helix-turn-ahelix motif, which is thought to be involved in DNA binding (amino acid residues 26 to 47; ISACKSON and BERTRAND1985). The region of the protein for which mutants have ...
... is represented by a linear bar with both the N- and C-terminal ends indicated. The solid portion defines the potential a-helix-turn-ahelix motif, which is thought to be involved in DNA binding (amino acid residues 26 to 47; ISACKSON and BERTRAND1985). The region of the protein for which mutants have ...
Resistance to Antibiotics Mediated by Target Alterations
... and penicillin-resistant gonococci and meningococci provided the first evidence that low-affinity PBPs arise by recombination rather than by mutation (18). The PBP 2 genes of penicillin-susceptible isolates of each species are very uniform in sequence, whereas those of resistant isolates have a mosa ...
... and penicillin-resistant gonococci and meningococci provided the first evidence that low-affinity PBPs arise by recombination rather than by mutation (18). The PBP 2 genes of penicillin-susceptible isolates of each species are very uniform in sequence, whereas those of resistant isolates have a mosa ...
(S) tet Resistance Determinant Element Containing the Tetracycline
... Southern blotting and hybridization were carried out with an ECL Direct Nucleic Acid Labeling and Detection system (Amersham Biosciences, Little Chalfont, United Kingdom). Southern blots were probed with pAM120 (Table 1) and PCR products derived from tet(S), tet(M), and the int and xis genes of Tn91 ...
... Southern blotting and hybridization were carried out with an ECL Direct Nucleic Acid Labeling and Detection system (Amersham Biosciences, Little Chalfont, United Kingdom). Southern blots were probed with pAM120 (Table 1) and PCR products derived from tet(S), tet(M), and the int and xis genes of Tn91 ...
Accelerated Evolution of Sex Chromosomes in
... set of autosomes and one of their X chromosomes to the progeny during sexual reproduction, we predict similar effective population sizes for X chromosome and autosomes (i.e., NeA 5 NeX), and so an X:A diversity ratio equal to one (this hypothesis is validated by simulations in the present study). He ...
... set of autosomes and one of their X chromosomes to the progeny during sexual reproduction, we predict similar effective population sizes for X chromosome and autosomes (i.e., NeA 5 NeX), and so an X:A diversity ratio equal to one (this hypothesis is validated by simulations in the present study). He ...
Future Dog Breeding for Genetic Soundness
... Every breeder is a keeper of the "genetic flame" for his or her breed. Every breeding decision an individual breeder makes has the potential to affect the future of the breed. For conscientious breeders, each attempt to produce quality purebred dogs includes a strong commitment to reduce inherited d ...
... Every breeder is a keeper of the "genetic flame" for his or her breed. Every breeding decision an individual breeder makes has the potential to affect the future of the breed. For conscientious breeders, each attempt to produce quality purebred dogs includes a strong commitment to reduce inherited d ...
The Arabidopsis WAVY GROWTH 2 Protein Modulates Root
... of the wild type. These results indicate that the mutation at the WAV2 locus causes an abnormality in the root bending in response to the touch stimulus at the root tip but that it does not cause severe defects in the root growth. Takahashi et al. (2002) reported that hydrotropic curvature was incre ...
... of the wild type. These results indicate that the mutation at the WAV2 locus causes an abnormality in the root bending in response to the touch stimulus at the root tip but that it does not cause severe defects in the root growth. Takahashi et al. (2002) reported that hydrotropic curvature was incre ...
Sexual Life Cycle and Meiosis
... Three events are unique to meiosis, and all three occur in meiosis l – Synapsis and crossing over in prophase I: Homologous chromosomes physically connect and exchange genetic information – At the metaphase plate, there are paired homologous chromosomes (tetrads), instead of individual replicated ch ...
... Three events are unique to meiosis, and all three occur in meiosis l – Synapsis and crossing over in prophase I: Homologous chromosomes physically connect and exchange genetic information – At the metaphase plate, there are paired homologous chromosomes (tetrads), instead of individual replicated ch ...
APC5 Antibody
... promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take ...
... promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take ...
View as PDF
... Silent information regulator 3 is an essential component of the Saccharomyces cerevisiae silencing complex that functions at telomeres and the silent mating-type loci, HMR and HML. We show that expression of the Nand C-terminal-encoding halves of SIR3 in trans partially complements the mating defect ...
... Silent information regulator 3 is an essential component of the Saccharomyces cerevisiae silencing complex that functions at telomeres and the silent mating-type loci, HMR and HML. We show that expression of the Nand C-terminal-encoding halves of SIR3 in trans partially complements the mating defect ...
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics
... LPP is a member of the zyxin family of proteins, which contains five members: ajuba, LIMD1, LPP, TRIP6 and zyxin. The family hallmark of these proteins are three clustered LIM domains at the carboxy-terminus, which are protein interaction domains. All family members are present at sites of cell adhe ...
... LPP is a member of the zyxin family of proteins, which contains five members: ajuba, LIMD1, LPP, TRIP6 and zyxin. The family hallmark of these proteins are three clustered LIM domains at the carboxy-terminus, which are protein interaction domains. All family members are present at sites of cell adhe ...
Determination of Protein Molecular Weight
... Proteins are a highly diversified class of biomolecules. Differences in their chemical properties, such as charge, functional groups, shape, size and solubility enable them to perform many biological functions. These functions include enzyme catalysis, metabolic regulation, binding and transport of ...
... Proteins are a highly diversified class of biomolecules. Differences in their chemical properties, such as charge, functional groups, shape, size and solubility enable them to perform many biological functions. These functions include enzyme catalysis, metabolic regulation, binding and transport of ...
Attanasio et al.
... may depend on its zinc fingers, which are highly similar in protein sequence to the zinc fingers of Gli2 and Gli3, which are also known to localize to cilia11. To gain insight into the physiologic and pathophysiological roles of the GLIS2 transcription factor, we analyzed mice with a targeted disrup ...
... may depend on its zinc fingers, which are highly similar in protein sequence to the zinc fingers of Gli2 and Gli3, which are also known to localize to cilia11. To gain insight into the physiologic and pathophysiological roles of the GLIS2 transcription factor, we analyzed mice with a targeted disrup ...
Cloning and characterization of an eukaryotic initiation factor
... kinase assay buffer (25 mM HEPES, pH 7.4, 25 mM h-glycerophosphate, 25 mM MgCl2, 2 mM dithiothreitol, 0.1 mM sodium orthovanadate) in the presence of g-32P-ATP (3000 Ci/mmol) at 30 jC for 30 min. Proteins were separated by SDS-PAGE and visualized by autoradiography. (B) Expression of BeK wildtype (B ...
... kinase assay buffer (25 mM HEPES, pH 7.4, 25 mM h-glycerophosphate, 25 mM MgCl2, 2 mM dithiothreitol, 0.1 mM sodium orthovanadate) in the presence of g-32P-ATP (3000 Ci/mmol) at 30 jC for 30 min. Proteins were separated by SDS-PAGE and visualized by autoradiography. (B) Expression of BeK wildtype (B ...
The Transition from Stiff to Compliant Materials in Squid Beaks
... enough to allow cutout specimens showing incremental differences in pigmentation (Fig. 1E), we performed coupled chemical and mechanical analyses to explore the consequences of pigmentation (figs. S1 and S2). We carried out chemical analyses of consecutive cutouts after degradation by two treatments ...
... enough to allow cutout specimens showing incremental differences in pigmentation (Fig. 1E), we performed coupled chemical and mechanical analyses to explore the consequences of pigmentation (figs. S1 and S2). We carried out chemical analyses of consecutive cutouts after degradation by two treatments ...
Transcription
... in DNA sequencing. It is used to identify the specific DNA sequences that are bound by a particular protein. ...
... in DNA sequencing. It is used to identify the specific DNA sequences that are bound by a particular protein. ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.