PDF - Molecular Cytogenetics
... meiosis, translocations tend to cause errors on chromosome disjunction and the ones involving sex chromosomes have particular implications for the phenotype. Male carriers of balanced X-autosome translocations are almost invariably infertile due to interruption of the spermatogenesis, but the mechan ...
... meiosis, translocations tend to cause errors on chromosome disjunction and the ones involving sex chromosomes have particular implications for the phenotype. Male carriers of balanced X-autosome translocations are almost invariably infertile due to interruption of the spermatogenesis, but the mechan ...
Wnt Signaling and an APC-Related Gene Specify Endoderm in
... In a wild-type embryo, POP-1 appears to be present at a high level in the MS nucleus and at a lower level in the E nucleus. In a mutant lacking detectable POP-1 in both MS and E, both blastomeres adopt E-like fates and produce endoderm. POP-1 is an HMG-domain protein similar to the vertebrate Tcf-1 ...
... In a wild-type embryo, POP-1 appears to be present at a high level in the MS nucleus and at a lower level in the E nucleus. In a mutant lacking detectable POP-1 in both MS and E, both blastomeres adopt E-like fates and produce endoderm. POP-1 is an HMG-domain protein similar to the vertebrate Tcf-1 ...
phylogenetic analysis of the rompb genes of rickettsia felis and
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
Attachment 2 - Food Standards Australia New Zealand
... sequence. Polymerase chain reaction (PCR) analyses were performed to verify the sequences at the 5' and 3' ends of the insert. Insert and copy number Southern hybridisation was used to determine the number and nature of DNA insertions in line H7-1. The genomic DNA was digested and probed with an int ...
... sequence. Polymerase chain reaction (PCR) analyses were performed to verify the sequences at the 5' and 3' ends of the insert. Insert and copy number Southern hybridisation was used to determine the number and nature of DNA insertions in line H7-1. The genomic DNA was digested and probed with an int ...
Microbiology 146:
... The isolation and characterization of a Rhizobium etli glutamate auxotroph, TAD12, harbouring a single Tn5 insertion, is reported. This mutant produced no detectable glutamate synthase (GOGAT) activity. The cloning and physical characterization of a 72 kb fragment of R. etli DNA harbouring the stru ...
... The isolation and characterization of a Rhizobium etli glutamate auxotroph, TAD12, harbouring a single Tn5 insertion, is reported. This mutant produced no detectable glutamate synthase (GOGAT) activity. The cloning and physical characterization of a 72 kb fragment of R. etli DNA harbouring the stru ...
Biochemistry - Textbooks Online
... of chemical reactions that occur in living cells and organisms. Life is a chemical process involving thousands of different reactions occurring in an organised manner. These are called metabolic reactions. The term Biochemistry was first introduced by the German Chemist Carl Neuberg in 1903. It take ...
... of chemical reactions that occur in living cells and organisms. Life is a chemical process involving thousands of different reactions occurring in an organised manner. These are called metabolic reactions. The term Biochemistry was first introduced by the German Chemist Carl Neuberg in 1903. It take ...
8. Principles of Genetics and Cytogenetics
... Historical background of genetics, theories and hypothesis. Physical basis of heredity, cell reproduction, mitosis, meiosis and its significance. Gametogenesis and syngamy in plants. Mendelian genetics–Mendel’s principles of heredity, deviation from Mendelian inheritance, pleiotropy, threshold chara ...
... Historical background of genetics, theories and hypothesis. Physical basis of heredity, cell reproduction, mitosis, meiosis and its significance. Gametogenesis and syngamy in plants. Mendelian genetics–Mendel’s principles of heredity, deviation from Mendelian inheritance, pleiotropy, threshold chara ...
Different packing of external residues can explain differences in the
... folding rate constants for these proteins are identical, but the unfolding rate for the protein from the hyperthermophilic organism is by an order of magnitude slower than that for the protein from the thermophilic organism (Olofsson et al., 2007). Along with experimental works, theoretical studies ...
... folding rate constants for these proteins are identical, but the unfolding rate for the protein from the hyperthermophilic organism is by an order of magnitude slower than that for the protein from the thermophilic organism (Olofsson et al., 2007). Along with experimental works, theoretical studies ...
New insight into plant intramembrane proteases
... proteins were described as inactive rhomboid-like homologs and 3 sequences (At2g41160, At3g07950 and At3g56740) were not analyzed in terms of clade membership (Lemberg and Freeman 2007). The second class of intramembrane proteases comprises site-2-proteases (S2P), zinc metalloproteases occurring wid ...
... proteins were described as inactive rhomboid-like homologs and 3 sequences (At2g41160, At3g07950 and At3g56740) were not analyzed in terms of clade membership (Lemberg and Freeman 2007). The second class of intramembrane proteases comprises site-2-proteases (S2P), zinc metalloproteases occurring wid ...
A Histone H3.3-like Gene Specifically Expressed in the Vegetative
... transcripts reached a maximum in mature pollen and were distributed uniformly in the cytoplasm of both the generative and vegetative cells. This observation is contrary to the common belief that histone H3 genes are specifically up-regulated during S phase of the cell cycle because neither type of c ...
... transcripts reached a maximum in mature pollen and were distributed uniformly in the cytoplasm of both the generative and vegetative cells. This observation is contrary to the common belief that histone H3 genes are specifically up-regulated during S phase of the cell cycle because neither type of c ...
The Diversity of Self-Incompatibility Systems in Flowering Plants
... putative pollen ligand in a concerted manner and were therefore likely to have co-evolved (Kusaba et al., 1997). However, subsequent studies showed that this close within-haplotype sequence similarity between SRK and SLG did not hold for all S haplotypes. The amino acid sequences of some SLGs and SR ...
... putative pollen ligand in a concerted manner and were therefore likely to have co-evolved (Kusaba et al., 1997). However, subsequent studies showed that this close within-haplotype sequence similarity between SRK and SLG did not hold for all S haplotypes. The amino acid sequences of some SLGs and SR ...
Lesson 1 – Introduction to Biotechnology
... Print the following sentence on the board: THE OLD RED DOG WAS TOO BIG FOR HIS BED. Each word consists of three letters like the triplet code of DNA. If a mutation were to cause a deletion of the D in OLD, the sentence would now read: THE OLR EDD OGW AST OOB IGF ORH ISB ED. Obviously this is nonsens ...
... Print the following sentence on the board: THE OLD RED DOG WAS TOO BIG FOR HIS BED. Each word consists of three letters like the triplet code of DNA. If a mutation were to cause a deletion of the D in OLD, the sentence would now read: THE OLR EDD OGW AST OOB IGF ORH ISB ED. Obviously this is nonsens ...
Contribution of defined amino acid residues to the immunogenicity
... STh have been mapped to a highly conserved domain including six cysteine residues forming three intramolecular disul¢de bonds that are absolutely necessary for toxicity of the molecule [5]. Because STa is non-immunogenic in its native form, several di¡erent approaches have been explored to obtain no ...
... STh have been mapped to a highly conserved domain including six cysteine residues forming three intramolecular disul¢de bonds that are absolutely necessary for toxicity of the molecule [5]. Because STa is non-immunogenic in its native form, several di¡erent approaches have been explored to obtain no ...
A Diurnal Component to the Variation in Sieve Tube Amino Acid
... significant evaporation would reduce the observed diameter of the exuding sap droplet, resulting in an overestimation of in vivo concentrations, a problem previously recognized by Fisher and Cash-Clarke (2000). Evaporation would be greater for more slowly exuding samples, as these require a longer p ...
... significant evaporation would reduce the observed diameter of the exuding sap droplet, resulting in an overestimation of in vivo concentrations, a problem previously recognized by Fisher and Cash-Clarke (2000). Evaporation would be greater for more slowly exuding samples, as these require a longer p ...
Misregulation of pre-mRNA splicing that causes human diseases
... F i g u r e 1 . cis and trans factors involved in pre-mRNA splicing. A) Elements involved in alternative splicing of pre-mRNA. Exons are indicated as boxes, introns as thin lines. Splicing regulator elements (enhancers or silencers) are shown as gray boxes in exons or as thin boxes in introns. The 5 ...
... F i g u r e 1 . cis and trans factors involved in pre-mRNA splicing. A) Elements involved in alternative splicing of pre-mRNA. Exons are indicated as boxes, introns as thin lines. Splicing regulator elements (enhancers or silencers) are shown as gray boxes in exons or as thin boxes in introns. The 5 ...
Molecular diversity of thermophilic bacteria isolated from Pasinler
... asidi, BOX PCR profilleme metodları ve 16S rDNA dizileme verileri kullanılmıştır. Morfolojik, fizyolojik ve biyokimyasal ...
... asidi, BOX PCR profilleme metodları ve 16S rDNA dizileme verileri kullanılmıştır. Morfolojik, fizyolojik ve biyokimyasal ...
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... which are effective against the most important Gram-positive organisms. Oxazolidinones inhibit initiation of the synthesis of bacterial proteins by preventing the formation of the ternary complex at the 70S ribosomal subunit[13]. Pharmacokinetic parameters for 600 mg oral doses of linezolid after m ...
... which are effective against the most important Gram-positive organisms. Oxazolidinones inhibit initiation of the synthesis of bacterial proteins by preventing the formation of the ternary complex at the 70S ribosomal subunit[13]. Pharmacokinetic parameters for 600 mg oral doses of linezolid after m ...
PDF - Oxford Academic - Oxford University Press
... set of prokaryotic RFs differing in stop codon specificity, RF1 for UAG and UAA, and RF2 for UGA and UAA, was analyzed by means of a recently developed computational method allowing identification of the specificity-determining positions (SDPs) in families composed of proteins with similar but not i ...
... set of prokaryotic RFs differing in stop codon specificity, RF1 for UAG and UAA, and RF2 for UGA and UAA, was analyzed by means of a recently developed computational method allowing identification of the specificity-determining positions (SDPs) in families composed of proteins with similar but not i ...
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... and the nucleotide sequence responsible for each terminal restriction fragment (T-RF) was determined by 16S rRNA gene cloning and sequencing [18]. Two major populations, one assigned to the genus Thauera, and another related to the genera Ralstonia and Limnobacter, were associated with 4-chlorobenzo ...
... and the nucleotide sequence responsible for each terminal restriction fragment (T-RF) was determined by 16S rRNA gene cloning and sequencing [18]. Two major populations, one assigned to the genus Thauera, and another related to the genera Ralstonia and Limnobacter, were associated with 4-chlorobenzo ...
The gene responsible for Clouston hidrotic
... inductive ectoderm–mesoderm reciprocal interactions (31) and because hearing loss has been reported in a few cases of HED (9), it is possible that these three diseases are caused by different mutations in the same gene or in related genes found in a cluster. The candidate region for the HED gene con ...
... inductive ectoderm–mesoderm reciprocal interactions (31) and because hearing loss has been reported in a few cases of HED (9), it is possible that these three diseases are caused by different mutations in the same gene or in related genes found in a cluster. The candidate region for the HED gene con ...
Diagnostic and Prognostic Significance of Gene Expression
... • Proto-oncogene located at 18q21 that promotes B-cell survival via inhibition of apoptosis and confers chemotherapy resistance • BCL-2 expression is normally down-regulated in the GC where apoptosis plays a critical role in negative B-cell selection • t(14;18) fusion gene leading to transcription ...
... • Proto-oncogene located at 18q21 that promotes B-cell survival via inhibition of apoptosis and confers chemotherapy resistance • BCL-2 expression is normally down-regulated in the GC where apoptosis plays a critical role in negative B-cell selection • t(14;18) fusion gene leading to transcription ...
Characterization of the binding properties of the Avian Coronavirus
... Schematic drawing of the IBV spike protein. Detection of soluble chimeric proteins derived from coronaviruses IBV-B1648 and SARS-CoV Binding of IBVS1Fc to chicken tiss ...
... Schematic drawing of the IBV spike protein. Detection of soluble chimeric proteins derived from coronaviruses IBV-B1648 and SARS-CoV Binding of IBVS1Fc to chicken tiss ...
Article On the Complexity of Chloroplast RNA
... For each mutant, we compared the growth properties of at least three lines rescued with the WT copy of the nuclear gene with those of two lines rescued with the intron-less psaA-i gene in the chloroplast (fig. 2A, supplementary figs. S2 and S3, Supplementary Material online). All the transformants ...
... For each mutant, we compared the growth properties of at least three lines rescued with the WT copy of the nuclear gene with those of two lines rescued with the intron-less psaA-i gene in the chloroplast (fig. 2A, supplementary figs. S2 and S3, Supplementary Material online). All the transformants ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.