Two Different Functions of Connexin43 Confer
... the stp gene. Surprisingly, we isolated a mutation in the connexin43 gene, which is known as the sof (short-of-fin) gene in zebrafish (21). sof is a well studied type 2 zebrafish mutant, in which fin segments are short in the caudal fin (21). Four sof mutant alleles were isolated by mutagenesis scre ...
... the stp gene. Surprisingly, we isolated a mutation in the connexin43 gene, which is known as the sof (short-of-fin) gene in zebrafish (21). sof is a well studied type 2 zebrafish mutant, in which fin segments are short in the caudal fin (21). Four sof mutant alleles were isolated by mutagenesis scre ...
Carnitine-acylcarnitine translocase deficiency: metabolic
... Glucose is the major source of energy for the fetus [1]. Immediately after birth free fatty acids are mobilized from adipose tissue stores. A rapid increase in the activity of carnitine palmitoyltransferase I and II and a rise in the capacity to oxidize fatty acids is found in liver [2] and in heart ...
... Glucose is the major source of energy for the fetus [1]. Immediately after birth free fatty acids are mobilized from adipose tissue stores. A rapid increase in the activity of carnitine palmitoyltransferase I and II and a rise in the capacity to oxidize fatty acids is found in liver [2] and in heart ...
Application title: Cloning genes for expression in AAV vectors for
... Wildtype AAV can site-specifically integrate into a defined site, AAVS1, on human chromosome 19. Recombinant AAV has lost this ability, as vectors do not express the rep gene products required for integration: recombinant AAV vectors only contain the 145 bp inverted terminal repeat (ITR) sequence fr ...
... Wildtype AAV can site-specifically integrate into a defined site, AAVS1, on human chromosome 19. Recombinant AAV has lost this ability, as vectors do not express the rep gene products required for integration: recombinant AAV vectors only contain the 145 bp inverted terminal repeat (ITR) sequence fr ...
variation in the strength and softness of selection on
... values that were most likely to have produced the observed numbers of surviving wild type and mutant individuals from demes of varying genetic composition. Various levels of uncertainty were inherent to the experimental design and these were incorporated into the model as described below. The framew ...
... values that were most likely to have produced the observed numbers of surviving wild type and mutant individuals from demes of varying genetic composition. Various levels of uncertainty were inherent to the experimental design and these were incorporated into the model as described below. The framew ...
Chapter 4: EXTENSIONS OF MENDELIAN INHERITANCE
... that obey two laws: the law of segregation and the law of independent assortment. Until now, we have mainly considered traits that are affected by a single gene that is found in two different alleles. In these cases, one allele is dominant over the other. This type of inheritance is sometimes called ...
... that obey two laws: the law of segregation and the law of independent assortment. Until now, we have mainly considered traits that are affected by a single gene that is found in two different alleles. In these cases, one allele is dominant over the other. This type of inheritance is sometimes called ...
Identification of fungal oxaloacetate hydrolyase within the
... oxidation of glyoxylate7 and glycolaldehyde.8 The acetoacetate/OAH route predominates in oxalate producers examined to date1,9–14 and therefore the oah gene might be used for the classification of fungi as potential oxalate producers. Recently, the genomes of a large number of oxalate and nonoxalate ...
... oxidation of glyoxylate7 and glycolaldehyde.8 The acetoacetate/OAH route predominates in oxalate producers examined to date1,9–14 and therefore the oah gene might be used for the classification of fungi as potential oxalate producers. Recently, the genomes of a large number of oxalate and nonoxalate ...
Physiological characterization of natural transformation in
... electrophoresis on a 0.8 YOagarose gel with single-stranded pAVA213-8 DNA as a marker. To concentrate and to get rid of RNA and some of the chromosomal DNA, fragments, between 2.5 and 11 kb in size, were isolated via electro-elution and after ethanol precipitation, resuspended in 20 p1 T50E20 (singl ...
... electrophoresis on a 0.8 YOagarose gel with single-stranded pAVA213-8 DNA as a marker. To concentrate and to get rid of RNA and some of the chromosomal DNA, fragments, between 2.5 and 11 kb in size, were isolated via electro-elution and after ethanol precipitation, resuspended in 20 p1 T50E20 (singl ...
Sequence Information Encoded in DNA that May Influence Long
... continuous chromatin organizing signals than in control regions. We also found a relationship between the meiotic recombination frequency and the presence of strong VWG chromatin organizing signals. Large ($300 kb) genomic DNA regions having low average recombination frequency are enriched in chroma ...
... continuous chromatin organizing signals than in control regions. We also found a relationship between the meiotic recombination frequency and the presence of strong VWG chromatin organizing signals. Large ($300 kb) genomic DNA regions having low average recombination frequency are enriched in chroma ...
The promiscuous primase
... with Pol b. It is a template-independent DNA and RNA polymerase that has a key role in the generation of antibody diversity by the V(D)J recombination process. In pre-T-cell extracts, TdT associates with Ku, a heterodimeric protein required for V(D)J recombination and for nonhomologous end joining ( ...
... with Pol b. It is a template-independent DNA and RNA polymerase that has a key role in the generation of antibody diversity by the V(D)J recombination process. In pre-T-cell extracts, TdT associates with Ku, a heterodimeric protein required for V(D)J recombination and for nonhomologous end joining ( ...
Fatty Acid Biosynthesis
... • FA longer than palmitic acid are synthesized by an elongation enzyme system. Additional carbons are added in 2-carbon units using malonyl CoA as the donor. • Unsaturated fatty acids are synthesized by the action of specific enzymes called as fatty acid CoA desaturases. The desaturases are specific ...
... • FA longer than palmitic acid are synthesized by an elongation enzyme system. Additional carbons are added in 2-carbon units using malonyl CoA as the donor. • Unsaturated fatty acids are synthesized by the action of specific enzymes called as fatty acid CoA desaturases. The desaturases are specific ...
Correlation of an Immobilized Digestive Enzyme Assay with Poultry
... The cost of feed represents >65% of the bird arriving at a US processing plant (July 2005 Agri Stats); therefore a more precise knowledge of the nutritional value of feed ingredients would enable the formulation of diets that more closely meet amino acid requirements. In vitro and in vivo techniques ...
... The cost of feed represents >65% of the bird arriving at a US processing plant (July 2005 Agri Stats); therefore a more precise knowledge of the nutritional value of feed ingredients would enable the formulation of diets that more closely meet amino acid requirements. In vitro and in vivo techniques ...
Molecular Evidence for Vector Implication of Onchocerca lupi in Los
... associated with ungulates, including horses and cattle. The causative agent of human “river blindness” also resides within the same genus (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). Historically, canids were not thought to be natural hosts for any Onchocerca parasite (Eberhard et al., 2000) unt ...
... associated with ungulates, including horses and cattle. The causative agent of human “river blindness” also resides within the same genus (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). Historically, canids were not thought to be natural hosts for any Onchocerca parasite (Eberhard et al., 2000) unt ...
Preference for and learning of amino acids in larval
... previous access to food. The preference scores differ across amino acids (Fig. 1A; for the underlying ratios of the number of animals in the inner circle versus total, see Fig. S2A). At the concentration used, significant preference was observed for half of the tested amino acids, including histidin ...
... previous access to food. The preference scores differ across amino acids (Fig. 1A; for the underlying ratios of the number of animals in the inner circle versus total, see Fig. S2A). At the concentration used, significant preference was observed for half of the tested amino acids, including histidin ...
secondary school improvement programme - Sci
... individual expresses both traits, example: blood groups. Protein A and protein B are coded by alleles A and B. If no protein A or B is present, then these cells will be coded by the allele O. This means that there are three possible alleles for this one gene locus in humans. When there are more than ...
... individual expresses both traits, example: blood groups. Protein A and protein B are coded by alleles A and B. If no protein A or B is present, then these cells will be coded by the allele O. This means that there are three possible alleles for this one gene locus in humans. When there are more than ...
champion petfoods white paper
... and cats – essential to basic body functions, including cellular regeneration, tissue maintenance, hormone and enzyme production, and the provision of energy. Although protein is essential, not all proteins function equally, with protein qualities varying enormously between various sources. Three fa ...
... and cats – essential to basic body functions, including cellular regeneration, tissue maintenance, hormone and enzyme production, and the provision of energy. Although protein is essential, not all proteins function equally, with protein qualities varying enormously between various sources. Three fa ...
Distinct Classes of Mitotic Cyclins Are Differentially
... Degenerated oligonucleotide primers were designed for a highly conserved region of published amino acid sequences of mitotic cyclins (Hata et al., 1991). Polymerase chain reaction (PCR) using these primers with the cDNAs from young developing nodules or root tips resulted in amplification of cDNA fr ...
... Degenerated oligonucleotide primers were designed for a highly conserved region of published amino acid sequences of mitotic cyclins (Hata et al., 1991). Polymerase chain reaction (PCR) using these primers with the cDNAs from young developing nodules or root tips resulted in amplification of cDNA fr ...
Quantification of Protein Levels in Single Living
... Accurate protein level measurement is important for biologists since many cellular phenotypes and molecular processes are sensitive to protein levels. However, the current assays for protein quantification have remained unchanged for several decades, and suffer from several limitations such as being ...
... Accurate protein level measurement is important for biologists since many cellular phenotypes and molecular processes are sensitive to protein levels. However, the current assays for protein quantification have remained unchanged for several decades, and suffer from several limitations such as being ...
The proton-linked monocarboxylate transporter (MCT) family
... velopment and in the adult chicken RPE [38]. These data imply regulation of MCT3 expression during development through the control of different promoters. Most recently, by searching the dbEST for fragments of MCTlike sequences, we identified four new potential members of the MCT family which were s ...
... velopment and in the adult chicken RPE [38]. These data imply regulation of MCT3 expression during development through the control of different promoters. Most recently, by searching the dbEST for fragments of MCTlike sequences, we identified four new potential members of the MCT family which were s ...
PDF - Hormones.gr
... may also be translated into eight receptor β isoforms through the same molecular mechanisms. The classic hGRα is a modular protein that consists of four functional domains: i) the amino-terminal or immunogenic domain (NTD), which is the largest domain of the receptor and consists of amino acids that ...
... may also be translated into eight receptor β isoforms through the same molecular mechanisms. The classic hGRα is a modular protein that consists of four functional domains: i) the amino-terminal or immunogenic domain (NTD), which is the largest domain of the receptor and consists of amino acids that ...
PLANT PROTEIN PHOSPHATASES
... Multiple PP1 isogenes are present in most eukaryotes, with the exception of S. cerevisiae which contains a single PP1, GLC7 (118). The physiological advantage of having multiple PP1 isogenes remains unknown. Disruption of the two S. pombe PP1 genes is lethal, but deletion of either gene, singly, has ...
... Multiple PP1 isogenes are present in most eukaryotes, with the exception of S. cerevisiae which contains a single PP1, GLC7 (118). The physiological advantage of having multiple PP1 isogenes remains unknown. Disruption of the two S. pombe PP1 genes is lethal, but deletion of either gene, singly, has ...
Complementary DNA Cloning, Messenger RNA
... has been tentatively identified, although relatively little is known about this protein's structure and function (5, 6). Each class consists of several closely related gene products which are capable of forming dimeric proteins with other gene products of the same class, but not with protein subunit ...
... has been tentatively identified, although relatively little is known about this protein's structure and function (5, 6). Each class consists of several closely related gene products which are capable of forming dimeric proteins with other gene products of the same class, but not with protein subunit ...
Updated slides on gene prediction
... Long vs. Short ORFs • Long open reading frames may be a gene – At random, we should expect one stop codon in every (64/3) ~= 21 codons – However, genes are usually much longer than this • A basic approach is to scan for ORFs whose length exceeds certain threshold – This is naïve because some genes ( ...
... Long vs. Short ORFs • Long open reading frames may be a gene – At random, we should expect one stop codon in every (64/3) ~= 21 codons – However, genes are usually much longer than this • A basic approach is to scan for ORFs whose length exceeds certain threshold – This is naïve because some genes ( ...
Effect of duodenal infusions of leucine on milk yield and plasma
... hay and supplemental mixture. Infusions of amino acids in Leucine consisted of methionine (12.6 g/day), lysine (20.7 g/day), histidine (10.7 g/day) and leucine (19.3 g/day). The composition of amino acid infusate in Control was the same except for leucine that was replaced with monosodium L-glutamat ...
... hay and supplemental mixture. Infusions of amino acids in Leucine consisted of methionine (12.6 g/day), lysine (20.7 g/day), histidine (10.7 g/day) and leucine (19.3 g/day). The composition of amino acid infusate in Control was the same except for leucine that was replaced with monosodium L-glutamat ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.