Topic guide 1.2: Enzymes
... that allow reactions to occur many times faster than uncatalysed reactions. Enzymes act on specific molecules called substrates. These substrate molecules bind to a region of the enzyme known as the active site. The specificity of the enzyme is due to the fact that the substrate and active site have ...
... that allow reactions to occur many times faster than uncatalysed reactions. Enzymes act on specific molecules called substrates. These substrate molecules bind to a region of the enzyme known as the active site. The specificity of the enzyme is due to the fact that the substrate and active site have ...
Fredrik Lysholm Bioinformatic methods for characterization of viral pathogens in metagenomic samples Linköping studies in science and technology
... virology. Fueled by the reduction in sequencing costs and the unbiased nature of shotgun sequencing, viral metagenomics has rapidly become the strategy of choice for this exploration. This thesis mainly focuses on improving key methods used in viral metagenomics as well as the complete viral charact ...
... virology. Fueled by the reduction in sequencing costs and the unbiased nature of shotgun sequencing, viral metagenomics has rapidly become the strategy of choice for this exploration. This thesis mainly focuses on improving key methods used in viral metagenomics as well as the complete viral charact ...
幻灯片 1
... Triglycerides combine with cholesterol and specific proteins to form chylomicrons(CM, 乳糜 微粒) for transport to other tissues. The fate of dietary phospholipids is similar to that of triacylglycerols. Pancreatic phospholipases secreted into the intestine catalyze the hydrolysis of phospholipids, which ...
... Triglycerides combine with cholesterol and specific proteins to form chylomicrons(CM, 乳糜 微粒) for transport to other tissues. The fate of dietary phospholipids is similar to that of triacylglycerols. Pancreatic phospholipases secreted into the intestine catalyze the hydrolysis of phospholipids, which ...
Chapter 1
... • Small amounts of acetoacetate spontaneously lose CO2 to produce acetone – This process can result in “acetone breath” often associated with uncontrolled diabetes mellitus ...
... • Small amounts of acetoacetate spontaneously lose CO2 to produce acetone – This process can result in “acetone breath” often associated with uncontrolled diabetes mellitus ...
Nutritional Application of Proportions - hansen
... Proportion is also useful in solving measurement problems that have to do with amounts of sodium, calories, fat, and protein in food or an amount in a drug dosage. The proportion will use the information in a scenario to solve for the unknown quantities in a specific amount. Example ...
... Proportion is also useful in solving measurement problems that have to do with amounts of sodium, calories, fat, and protein in food or an amount in a drug dosage. The proportion will use the information in a scenario to solve for the unknown quantities in a specific amount. Example ...
Identification and temporal expression of putative circadian clock
... values (transcripts per million) were calculated following Wagner, Kin & Lynch (2012). Quantitative PCR methods for TPM validation are given in File S1 and primers in Table S1. All data files for time-course RNAseq can be found on the NCBI SRA data base, bioproject Accession No. 297565. ...
... values (transcripts per million) were calculated following Wagner, Kin & Lynch (2012). Quantitative PCR methods for TPM validation are given in File S1 and primers in Table S1. All data files for time-course RNAseq can be found on the NCBI SRA data base, bioproject Accession No. 297565. ...
Phase-I metabolism
... Oxidative Phase-I involving cytochrome P-450 enzymes: • Aliphatic hydroxylation: – Mainly occur on the ultimate (ω) or penultimate (ω-1) carbon atom in the structure. – Also it occurs at an activated carbon atom, that is next to sp , sp2 carbons: ...
... Oxidative Phase-I involving cytochrome P-450 enzymes: • Aliphatic hydroxylation: – Mainly occur on the ultimate (ω) or penultimate (ω-1) carbon atom in the structure. – Also it occurs at an activated carbon atom, that is next to sp , sp2 carbons: ...
Determination of Amino Acids in Wort and Beer by Reverse
... Key words: Amino acids analysis, Beer, High-performance liquid chromatography, Precolumn derivatization, Wort An adequate amino acid content in wort is necessary to promote yeast budding and energetic fermentation. It also influences the biological stability and color of the wort and, to a certain e ...
... Key words: Amino acids analysis, Beer, High-performance liquid chromatography, Precolumn derivatization, Wort An adequate amino acid content in wort is necessary to promote yeast budding and energetic fermentation. It also influences the biological stability and color of the wort and, to a certain e ...
Crystal structure of yeast hexokinase Pl in complex
... Hexokinase (ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1; HK) is a member of the kinase family of tissue-specific isoenzymes. Saccharomyces cerevisiae genome codify for two isoenzymes of hexokinase: PI and PII, which are about 76% similar in their amino-acid sequences.1,2 Isoenzyme PII has a total ...
... Hexokinase (ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1; HK) is a member of the kinase family of tissue-specific isoenzymes. Saccharomyces cerevisiae genome codify for two isoenzymes of hexokinase: PI and PII, which are about 76% similar in their amino-acid sequences.1,2 Isoenzyme PII has a total ...
Introduction to Carbohydrates
... • In contrast, the essential amino acids cannot be synthesized (or produced in sufficient amounts) by the body and, therefore, must be obtained from the diet in order for normal protein synthesis to occur. • Genetic defects in the pathways of amino acid metabolism can cause serious disease. ...
... • In contrast, the essential amino acids cannot be synthesized (or produced in sufficient amounts) by the body and, therefore, must be obtained from the diet in order for normal protein synthesis to occur. • Genetic defects in the pathways of amino acid metabolism can cause serious disease. ...
Free amino acids as phagostimulants in cricket nuptial gifts: support
... Figure 1. Millimolar concentrations of free amino acids in the spermatophylax (mean with 95% confidence interval). Essential amino acids (Dadd 1985) are marked with an asterisk. A mixture of proline (40.9% of the amino acid mixture), glycine (24.5%), arginine (26.5%) and alanine (8.1%) was incorpora ...
... Figure 1. Millimolar concentrations of free amino acids in the spermatophylax (mean with 95% confidence interval). Essential amino acids (Dadd 1985) are marked with an asterisk. A mixture of proline (40.9% of the amino acid mixture), glycine (24.5%), arginine (26.5%) and alanine (8.1%) was incorpora ...
Aspects of Reductive Explanation in Biological Science: Intrinsicality
... applicable if not also relevant to current research. The philosophical task is to explicate the reasoning in particular areas of biology and understand the diverse standards used by scientists to assess whether reductive explanations are successful, not to be for or against reductionism per se. Redu ...
... applicable if not also relevant to current research. The philosophical task is to explicate the reasoning in particular areas of biology and understand the diverse standards used by scientists to assess whether reductive explanations are successful, not to be for or against reductionism per se. Redu ...
Final Thesis Solid-phase bio-organic synthesis to create intelligent surfaces Patrik Nygren
... and still is, a major research field. Well-defined surfaces can be used to investigate biological systems (protein chips), to create nano-devices (nano-electronics), to design new materials etc. the applications are almost endless. Examples in the literature include the growth of inorganic crystals ...
... and still is, a major research field. Well-defined surfaces can be used to investigate biological systems (protein chips), to create nano-devices (nano-electronics), to design new materials etc. the applications are almost endless. Examples in the literature include the growth of inorganic crystals ...
Molecular Characterization of NADH-Dependent
... NAD(P)H-GOGATare highly unstable and occur in Iow abundance. Root nodule NADH-GOGAT from lupine (Benny and Boland, 1977), common bean (Chen and Cullimore, 1988, 1989), and alfalfa (Anderson et al., 1989) has been isolated and characterized, and its activity has been found to increase markedly during ...
... NAD(P)H-GOGATare highly unstable and occur in Iow abundance. Root nodule NADH-GOGAT from lupine (Benny and Boland, 1977), common bean (Chen and Cullimore, 1988, 1989), and alfalfa (Anderson et al., 1989) has been isolated and characterized, and its activity has been found to increase markedly during ...
HIS-Select Nickel Affinity Gel (P6611) - Technical - Sigma
... The HIS-Select Nickel Affinity Gel is stored in 30% ethanol. The ethanol must be removed just prior to use. Thoroughly resuspend the affinity gel with gentle inversion and remove an appropriate aliquot for use. Take only the amount of affinity gel that is necessary for the purification to be done. T ...
... The HIS-Select Nickel Affinity Gel is stored in 30% ethanol. The ethanol must be removed just prior to use. Thoroughly resuspend the affinity gel with gentle inversion and remove an appropriate aliquot for use. Take only the amount of affinity gel that is necessary for the purification to be done. T ...
Enzymes
... which enzyme acts. Substrate binds with the enzyme's active site and enzyme-substrate complex formed. More substrate in a solution, the greater the rate of the reaction Enzymes have more product with which to react. B-3.03 -- Enzymes ...
... which enzyme acts. Substrate binds with the enzyme's active site and enzyme-substrate complex formed. More substrate in a solution, the greater the rate of the reaction Enzymes have more product with which to react. B-3.03 -- Enzymes ...
nmr.evilia.190303
... In structure work, J coupling is critical to measuring the bond angles and dihedral angles between nuclei. The Karplus equation is used to calculate the dihedral angles for 3 bond or vicinal coupling: J = A + B cosq+ C cos2q Where A, B, C are coefficients that depend on the nuclei ...
... In structure work, J coupling is critical to measuring the bond angles and dihedral angles between nuclei. The Karplus equation is used to calculate the dihedral angles for 3 bond or vicinal coupling: J = A + B cosq+ C cos2q Where A, B, C are coefficients that depend on the nuclei ...
Development of Biocatalysts for Production of Fine Chemicals
... (K245R). In A8-39, the amino acid position 271 had aspartic acid substituted for asparagine (N271D), and in T1-99, there were two amino acid substitutions such ...
... (K245R). In A8-39, the amino acid position 271 had aspartic acid substituted for asparagine (N271D), and in T1-99, there were two amino acid substitutions such ...
Analysis of expressed sequence tags from Cryptomeria japonica
... Total RNA was isolated from pollen grains as described by Sone et al. (1994) and from other plant organs as described by Shinohara and Murakami (1996). Genomic DNA was extracted from current needles as described by Wagner et al. (1987). DNA and RNA gel blot analyses were performed as described previ ...
... Total RNA was isolated from pollen grains as described by Sone et al. (1994) and from other plant organs as described by Shinohara and Murakami (1996). Genomic DNA was extracted from current needles as described by Wagner et al. (1987). DNA and RNA gel blot analyses were performed as described previ ...
Amino Acids - faculty at Chemeketa
... Solution Match the end products of digestion with the types of food: 1. amino acids 2. fatty acids and glycerol 3. glucose A. fats B. proteins C. carbohydrates ...
... Solution Match the end products of digestion with the types of food: 1. amino acids 2. fatty acids and glycerol 3. glucose A. fats B. proteins C. carbohydrates ...
- Gastroenterology
... different trypsin activities in the pancreas were not due to different expression levels of trypsinogen because Ctsb⫺/⫺, Ctsl⫺/⫺, and wild-type mice had comparable trypsinogen levels in the pancreas under resting conditions (not shown). The observation was made even more puzzling by the fact that re ...
... different trypsin activities in the pancreas were not due to different expression levels of trypsinogen because Ctsb⫺/⫺, Ctsl⫺/⫺, and wild-type mice had comparable trypsinogen levels in the pancreas under resting conditions (not shown). The observation was made even more puzzling by the fact that re ...
Comparative Analysis of Prothrombin Activators
... the venom of P. textilis, have been isolated and the genes cloned (Rao, Swarup, and Kini 2003, 2004). Pseutarin C comprises approximately 40% of the total dry weight of the venom of P. textilis and its injection results in massive disseminated intravascular coagulation within the body of its prey, u ...
... the venom of P. textilis, have been isolated and the genes cloned (Rao, Swarup, and Kini 2003, 2004). Pseutarin C comprises approximately 40% of the total dry weight of the venom of P. textilis and its injection results in massive disseminated intravascular coagulation within the body of its prey, u ...
The Hypothesis that the Genetic Code Originated in Coupled
... for respiration [44]. NO produced in the atmosphere could have undergone reactions leading to accumulation of nitrite and nitrate in water [45]. If primitive cells were leaky to ions, nitrite and nitrate could diffuse across membranes and be reduced to ammonia using Fe(II) or FeS [46–48]. Ethanethio ...
... for respiration [44]. NO produced in the atmosphere could have undergone reactions leading to accumulation of nitrite and nitrate in water [45]. If primitive cells were leaky to ions, nitrite and nitrate could diffuse across membranes and be reduced to ammonia using Fe(II) or FeS [46–48]. Ethanethio ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.