Ageing and the aggregating proteoglycans of
... constant with age (Fig. 1).In the human [50-551, such age changes are characterized by (a) a decrease in the size and number of chondroitin sulphate chains, together with an increase in 6-sulphation relative to 4-sulphation, (b) an increase in the size and number of keratan sulphate chains, and a co ...
... constant with age (Fig. 1).In the human [50-551, such age changes are characterized by (a) a decrease in the size and number of chondroitin sulphate chains, together with an increase in 6-sulphation relative to 4-sulphation, (b) an increase in the size and number of keratan sulphate chains, and a co ...
USGENE - Chemical Abstracts Service
... The GETSIM and BLAST run packages are available to search the USGENE database for protein and nucleotide sequence data by similarity (homology). BLAST is provided in USGENE with the permission of the National Center for Biotechnology Information (NCBI) of the National Library of Medicine (NLM). GETS ...
... The GETSIM and BLAST run packages are available to search the USGENE database for protein and nucleotide sequence data by similarity (homology). BLAST is provided in USGENE with the permission of the National Center for Biotechnology Information (NCBI) of the National Library of Medicine (NLM). GETS ...
Combining docking and molecular dynamic simulations in drug design
... The three dimensional (3-D) structure of both ligand and protein are necessary for the application of docking techniques. While the manifold of conformational structures of small molecules may be relatively easy to predict, the lowest energy conformation obtained may not correspond to that of the bo ...
... The three dimensional (3-D) structure of both ligand and protein are necessary for the application of docking techniques. While the manifold of conformational structures of small molecules may be relatively easy to predict, the lowest energy conformation obtained may not correspond to that of the bo ...
Soybean Meal – An Exceptional Protein Source
... poultry. In fact, soybean meal is sometimes referred to as the "gold standard" because other protein sources are often compared to it. Soybean meal is rich in highly digestible protein, and the protein is composed of a superior blend of amino acids, the building blocks of body protein for livestock ...
... poultry. In fact, soybean meal is sometimes referred to as the "gold standard" because other protein sources are often compared to it. Soybean meal is rich in highly digestible protein, and the protein is composed of a superior blend of amino acids, the building blocks of body protein for livestock ...
Comparative Sequence Analysis of the Genomic Segment 6 of Four
... the Western blot technique monoclonal antibodies specific for VP6 epitopes shared by all rotaviruses were observed to react with both monomeric and trimeric forms of VP6, while monoclonal antibodies specific for a subgroup I or II epitope reacted only with the trimeric form of VP6. This observation ...
... the Western blot technique monoclonal antibodies specific for VP6 epitopes shared by all rotaviruses were observed to react with both monomeric and trimeric forms of VP6, while monoclonal antibodies specific for a subgroup I or II epitope reacted only with the trimeric form of VP6. This observation ...
Functional analysis of the human CDC5L complex
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
Studies on the key amino acid residues responsible for the alkali
... carried out by using three pairs of primer (primers 1 and 4, primers 2 and 3, primers 1 and 2, respectively). The mutant fragments were then cloned into plasmid pUC19 and more than 300 recombinants, which might included all 20 kinds of different amino acids substitutions of Asn-71, were selected and ...
... carried out by using three pairs of primer (primers 1 and 4, primers 2 and 3, primers 1 and 2, respectively). The mutant fragments were then cloned into plasmid pUC19 and more than 300 recombinants, which might included all 20 kinds of different amino acids substitutions of Asn-71, were selected and ...
Plant toxic proteins and their current significance for warfare and
... glycoprotein with carbohydrate side chains in the form of mannose-rich N-linked oligosaccharides and particularly binds to mannose receptors of cells of the reticuloendothelial system. The specific sites with potential for binding of high mannose carbohydrate chains of ricin are at asparagines 10 an ...
... glycoprotein with carbohydrate side chains in the form of mannose-rich N-linked oligosaccharides and particularly binds to mannose receptors of cells of the reticuloendothelial system. The specific sites with potential for binding of high mannose carbohydrate chains of ricin are at asparagines 10 an ...
CP1 Domain in Escherichia coli Leucyl
... On the basis of their conserved amino acid sequences and crystal structures, aaRSs are divided into two major classes, class I and II, each with characteristic sequences and structural motifs that form the substrate binding sites and catalytic sites (12-15). The 10 class I enzymes share HIGH and KMS ...
... On the basis of their conserved amino acid sequences and crystal structures, aaRSs are divided into two major classes, class I and II, each with characteristic sequences and structural motifs that form the substrate binding sites and catalytic sites (12-15). The 10 class I enzymes share HIGH and KMS ...
Studies on the structure and function of 16S ribosomal RNA using
... (iii) An unexpectedly stable structure has been identified in the region between positions 109 and 279, where many residues remain unreactive even at 90°C in EDTAcontaining buffer. This region may correspond to a structural 'core' that is important for early events in ribosome assembly (Garrett et a ...
... (iii) An unexpectedly stable structure has been identified in the region between positions 109 and 279, where many residues remain unreactive even at 90°C in EDTAcontaining buffer. This region may correspond to a structural 'core' that is important for early events in ribosome assembly (Garrett et a ...
Document
... growth conditions) and becomes the NMR-visible component. The other protein is expressed in an unlabeled form, after switching the medium to non-labeled growth conditions and thus remains NMR-invisible. Once the two proteins encounter each other in the cytoplasm of the expression host, the resulting ...
... growth conditions) and becomes the NMR-visible component. The other protein is expressed in an unlabeled form, after switching the medium to non-labeled growth conditions and thus remains NMR-invisible. Once the two proteins encounter each other in the cytoplasm of the expression host, the resulting ...
Production of recombinant EMA-1 protein and its
... respectively. The production of the recombinant histidine-tagged protein (His6-EMA1) was first evaluated in a small scale (50 mL) assay using 2YT medium containing 0.2% glucose and 30 μg.mL–1 kanamycin. The production of His6-EMA1 (34.2 kDa) was performed at 250 rev min–1 and both temperature of gro ...
... respectively. The production of the recombinant histidine-tagged protein (His6-EMA1) was first evaluated in a small scale (50 mL) assay using 2YT medium containing 0.2% glucose and 30 μg.mL–1 kanamycin. The production of His6-EMA1 (34.2 kDa) was performed at 250 rev min–1 and both temperature of gro ...
GC-content of synonymous codons profoundly influences amino
... Amino acids are typically encoded by multiple synonymous codons that are not used with the same frequency. Codon usage bias has drawn considerable attention, and several explanations have been offered including variation in GC-content between species. Focusing on a simple parameter - combined GC-pro ...
... Amino acids are typically encoded by multiple synonymous codons that are not used with the same frequency. Codon usage bias has drawn considerable attention, and several explanations have been offered including variation in GC-content between species. Focusing on a simple parameter - combined GC-pro ...
Structure and mechanism of ATP-dependent phospholipid transporters
... Fig. 1. General structure of ABC transporters and P-type ATPases. (A) Secondary structure of ABC transporters. The ABC transporter exists both as a monomer full transporter and as a dimer consisting of two half transporters. In either case, this type of transporter contains two transmembrane domains ...
... Fig. 1. General structure of ABC transporters and P-type ATPases. (A) Secondary structure of ABC transporters. The ABC transporter exists both as a monomer full transporter and as a dimer consisting of two half transporters. In either case, this type of transporter contains two transmembrane domains ...
Molecular identification of three Arabidopsis thaliana mitochondrial
... So far, several plant mitochondrial carriers have been identified by their high similarity to orthologues in other organisms [8–10], their transport properties upon heterologous expression and reconstitution into liposomes [11–15] and by functional complementation of yeast mutants [13,16,17]. Howeve ...
... So far, several plant mitochondrial carriers have been identified by their high similarity to orthologues in other organisms [8–10], their transport properties upon heterologous expression and reconstitution into liposomes [11–15] and by functional complementation of yeast mutants [13,16,17]. Howeve ...
... 3. (10 pts) The amino acid sequence for secondary structures that are found at the exterior of water soluble proteins have a well defined distribution, or order, of polar and non-polar residues. i) What is that distribution, for both -helices and -sheets. Briefly justify your answer with reference ...
HRP - WordPress.com
... Gel-Filtration Column This reduced volume of concentrated sample was further purified using a gel-filtration column. Gel-filtration separates proteins based on size and the path each protein takes as it passes through the resin bed. Large proteins will pass quickly down the column without entering t ...
... Gel-Filtration Column This reduced volume of concentrated sample was further purified using a gel-filtration column. Gel-filtration separates proteins based on size and the path each protein takes as it passes through the resin bed. Large proteins will pass quickly down the column without entering t ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.