The use of deuteration for the structural study of larger proteins
... Weak dependence on secondary structure: 13Cα : –0.5 ± 0.08 α-helical; -0.44 ± 0.08 β-strands No significant shifts for 1HN and 13CO (Δ values are based on HCA II (Venters, R. A.; Farmer, B. T.; Fierke, C. A.; Spicer, L. D. J. Mol. Biol. 1996, 264, 1101-1116.)) ...
... Weak dependence on secondary structure: 13Cα : –0.5 ± 0.08 α-helical; -0.44 ± 0.08 β-strands No significant shifts for 1HN and 13CO (Δ values are based on HCA II (Venters, R. A.; Farmer, B. T.; Fierke, C. A.; Spicer, L. D. J. Mol. Biol. 1996, 264, 1101-1116.)) ...
Protein Sequencing Problems
... sequence. Lys has an NH2 on its side chain. That is why FDNB attacked it. Chain = Gly ~ Carb A – Carb A attacks the AA on the other end of the protein (the carboxylic acid side). Since Ser was released, it must have been the last AA in the chain. Chain = Gly ~~~~~~~~~~~~~~~~~~~~~~~~~~Ser Cyanobromid ...
... sequence. Lys has an NH2 on its side chain. That is why FDNB attacked it. Chain = Gly ~ Carb A – Carb A attacks the AA on the other end of the protein (the carboxylic acid side). Since Ser was released, it must have been the last AA in the chain. Chain = Gly ~~~~~~~~~~~~~~~~~~~~~~~~~~Ser Cyanobromid ...
Chapter 14 Preparing Semisynthetic and Fully Synthetic Histones
... suitable for sensitive biochemical or biophysical characterization of nucleosomes. However, it is the only method by which any combination of PTMs may be introduced across the protein sequence and is limited in scope only by the ability to prepare the appropriate modified peptide segments. This tech ...
... suitable for sensitive biochemical or biophysical characterization of nucleosomes. However, it is the only method by which any combination of PTMs may be introduced across the protein sequence and is limited in scope only by the ability to prepare the appropriate modified peptide segments. This tech ...
Phylogenetic and Conservational Analyses of Dengue Non
... strains of each DENV serotype were grouped into four main geographical locations, Africa, South America, South East Asia and China. DENV 1 and 3 were phylogenetically closer among the four serotypes. They further showed more than 80% conservation between each other. DENV 4 showed a higher deviation ...
... strains of each DENV serotype were grouped into four main geographical locations, Africa, South America, South East Asia and China. DENV 1 and 3 were phylogenetically closer among the four serotypes. They further showed more than 80% conservation between each other. DENV 4 showed a higher deviation ...
Herculex® I Product Overview
... need for spray pesticides. In field tests, corn hybrids with Herculex I insect protection averaged 8.3-13.9 bu/acres more than the same hybrids without protection. This means an increased yield of 5 to 9 percent for farmers. With Herculex I insect protection, farmers can use fewer spray pesticides. ...
... need for spray pesticides. In field tests, corn hybrids with Herculex I insect protection averaged 8.3-13.9 bu/acres more than the same hybrids without protection. This means an increased yield of 5 to 9 percent for farmers. With Herculex I insect protection, farmers can use fewer spray pesticides. ...
University of Groningen Cross-linking of dimeric CitS and GltS
... sequence. The BAD encoding sequence was excised from pSB260 and ligated into the unique NcoI site of pBADCitS situated in between the enterokinase site and CitS encoding sequences yielding plasmid pCitSBAD1. Plasmid pCitS-BAD260 was obtained by replacing a Bpu1102I-XbaI fragment of pBADCitS with the ...
... sequence. The BAD encoding sequence was excised from pSB260 and ligated into the unique NcoI site of pBADCitS situated in between the enterokinase site and CitS encoding sequences yielding plasmid pCitSBAD1. Plasmid pCitS-BAD260 was obtained by replacing a Bpu1102I-XbaI fragment of pBADCitS with the ...
Sense codon emancipation for proteomewide incorporation of
... mitochondrial genomes, clearly indicate that in the context of the smaller (‘minimal’) genome and proteome, such reassignments in the code structure are more likely, as lethal effects are less possible (Budisa, 2005). In other living beings, species-specific reassignments are sometimes employed as b ...
... mitochondrial genomes, clearly indicate that in the context of the smaller (‘minimal’) genome and proteome, such reassignments in the code structure are more likely, as lethal effects are less possible (Budisa, 2005). In other living beings, species-specific reassignments are sometimes employed as b ...
Effects of Amino Acids Replacing Nitrate on Growth - dl.edi
... (Gly), serine (Ser), threonine (Thr), cysteine (Cys), tyrosine (Tyr), asparagine (Asn), and glutamine (Gln). All treatments had the same total 12.5 mmol L−1 N concentration in nutrient solutions. During the experiment, pH in nutrient solutions was maintained at about 6.0 by addition of either 1 mmol ...
... (Gly), serine (Ser), threonine (Thr), cysteine (Cys), tyrosine (Tyr), asparagine (Asn), and glutamine (Gln). All treatments had the same total 12.5 mmol L−1 N concentration in nutrient solutions. During the experiment, pH in nutrient solutions was maintained at about 6.0 by addition of either 1 mmol ...
The Activity of a Developmentally Regulated Cysteine Proteinase Is
... release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and characterized: CWP1 (26 kDa) and CWP2 (39 kDa). Expression ...
... release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and characterized: CWP1 (26 kDa) and CWP2 (39 kDa). Expression ...
The Incorporation of Glycerol and Lysine into the Lipid Fraction of
... fraction of a number of species of bacteria contains, in addition to neutral lipid, phospholipids of which, in Gram-positive organisms, di- and mono-phosphatidylglycerol are the main components. Later studies (Macfarlane, 1962b, 1964) showed that in Clostridium welchii and Staphylococcus aureus (Mic ...
... fraction of a number of species of bacteria contains, in addition to neutral lipid, phospholipids of which, in Gram-positive organisms, di- and mono-phosphatidylglycerol are the main components. Later studies (Macfarlane, 1962b, 1964) showed that in Clostridium welchii and Staphylococcus aureus (Mic ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... study indicate that the different sizes of these transcripts ...
... study indicate that the different sizes of these transcripts ...
eg1
... that includes multiple forms of all three classes when grown on crystalline cellulose (Cai et al. 1994, 1999). In addition to EG1, four other CMC-hydrolysing proteins were separated in lower yields from culture fluids of V. volvacea grown on crystalline cellulose using a combination of hydrophobic i ...
... that includes multiple forms of all three classes when grown on crystalline cellulose (Cai et al. 1994, 1999). In addition to EG1, four other CMC-hydrolysing proteins were separated in lower yields from culture fluids of V. volvacea grown on crystalline cellulose using a combination of hydrophobic i ...
Structural Basis for Bivalent Smac-Mimetics Recognition in the IAP
... composed of eight BIR3 molecules (XIAP residues 253 ± 2 through 351 ± 5) and eight compound 3 molecules, each of which has an inhibitory head bound to BIR3 and the other devoid of any contact to the protein. The eight asymmetric unit copies of the XIAP-BIR3/compound 3 complex can be divided into two ...
... composed of eight BIR3 molecules (XIAP residues 253 ± 2 through 351 ± 5) and eight compound 3 molecules, each of which has an inhibitory head bound to BIR3 and the other devoid of any contact to the protein. The eight asymmetric unit copies of the XIAP-BIR3/compound 3 complex can be divided into two ...
Sample pages 1 PDF
... (Fig. 2.4). In bacteria, the 30S subunit directly binds mRNA in the vicinity of the start codon. This process is mediated by the Shine–Dalgarno sequence, a unique feature of bacterial mRNAs that is located upstream of the start codon (Shine and Dalgarno 1974). This sequence interacts with a compleme ...
... (Fig. 2.4). In bacteria, the 30S subunit directly binds mRNA in the vicinity of the start codon. This process is mediated by the Shine–Dalgarno sequence, a unique feature of bacterial mRNAs that is located upstream of the start codon (Shine and Dalgarno 1974). This sequence interacts with a compleme ...
Protonation States and pKa
... Protein is positively charged (pI = 11) between pH 1 to pH 7 (titration range). This results in an overall decrease in the stability of the positively charged histidine residues and increase in the stability of the negatively charged Asp and Glu residues. Therefore, a decrease in the pKa values is ...
... Protein is positively charged (pI = 11) between pH 1 to pH 7 (titration range). This results in an overall decrease in the stability of the positively charged histidine residues and increase in the stability of the negatively charged Asp and Glu residues. Therefore, a decrease in the pKa values is ...
Cyclophilin
... CypA is the most famous one which has a beta barrel structure with two alpha helices and a betasheet. Cyclophilin is not born to bind to CsA Cyclophilin A is a cytosolic and abundant protein (0.1% of total cellular protein). Possesses a peptidyl prolyl isomerase(PPIase) activity, which catalyzes the ...
... CypA is the most famous one which has a beta barrel structure with two alpha helices and a betasheet. Cyclophilin is not born to bind to CsA Cyclophilin A is a cytosolic and abundant protein (0.1% of total cellular protein). Possesses a peptidyl prolyl isomerase(PPIase) activity, which catalyzes the ...
Interactions of liposomes and lipid-based carrier systems with blood
... majority of plasma proteins carry a net negative charge at physiological pH. Anionic lipids have been incorporated into liposomes to increase the trapped volume and improve the loading of various molecules and drugs, such as plasmids. In our studies the incorporation of 20 mol% negative surface char ...
... majority of plasma proteins carry a net negative charge at physiological pH. Anionic lipids have been incorporated into liposomes to increase the trapped volume and improve the loading of various molecules and drugs, such as plasmids. In our studies the incorporation of 20 mol% negative surface char ...
The proposed channel-enzyme transient receptor potential
... ADPRases, with turnover rates sufficient to cause complete – or near-complete – conversion of ADPR into AMP and R5P within this time frame (Figure 4A and B; R5P is invisible on the TLC). In contrast, Chi3S, 4S and NUDT9H (which contain the NUDT9H-type Nudix box, Figure 3A) all failed to hydrolyze th ...
... ADPRases, with turnover rates sufficient to cause complete – or near-complete – conversion of ADPR into AMP and R5P within this time frame (Figure 4A and B; R5P is invisible on the TLC). In contrast, Chi3S, 4S and NUDT9H (which contain the NUDT9H-type Nudix box, Figure 3A) all failed to hydrolyze th ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.