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energy - Bide Sport
energy - Bide Sport

... – Glucomannan drink with choline to support the fat metabolism – With choline to support the fat metabolism – Enriched with plant extracts from green tea, coffee, melon – Glucomannan contributes to weight reduction. Daily intake of 3 x 1 g konjac glucomannan in combination with water Flavours: B ...
Slides
Slides

... and (due to the evolutionary connection) have similar function The sequence alignment problem is an optimization problem: produce the best alignment according to a scoring function A scoring function provide numeric values for each possible symbol pairing and for gaps in an alignment. ...
Homology Modeling via Protein Threading - lmm
Homology Modeling via Protein Threading - lmm

... Let I0 be the maximum number of gap symbols placed before the first residue of S0 in any of the alignments A(S0; S1); : : : ;A(S0; SN). Let IS0j be the maximum number of gaps placed after the last character of S0 in any of the alignments, and let Ii be the maximum number of gaps placed between chara ...
Optimizing cofactor availability for the production of recombinant
Optimizing cofactor availability for the production of recombinant

... production landscapes from microscale cultivations using 1 mM FeSO4-supplemented medium. Error bars are standard deviations from all measured clones of a landscape. Average activity from strains producing HRP alone was set to 100%. In addition to strong constitutive co-overexpression of either HEM1 ...
Resistance to the Pseudomonas syringae Effector
Resistance to the Pseudomonas syringae Effector

... The Pseudomonas syringae-Arabidopsis (Arabidopsis thaliana) interaction is an extensively studied plant-pathogen system. Arabidopsis possesses approximately 150 putative resistance genes encoding nucleotide binding site (NBS) and leucine-rich repeat (LRR) domain-containing proteins. The majority of ...
Localization of Protein-Protein lnteractions between Subunits of
Localization of Protein-Protein lnteractions between Subunits of

... Rr value as a relative measure of each fusion protein's dimerization coefficient. Segments encoding pieces of the carboxy-terminal half of oat phytochrome were excised from pCIB315 (Thompson et al., 1989) and inserted into pME10. Initially, an Xmnl fragment encoding amino acids V623-E1048, nearly al ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... • Five genes code for the polypeptides in the enzymes of tryptophan synthesis • The trp operator lies wholly within the trp promoter • High tryptophan concentration is the signal to turn off the operon • Presence of tryptophan helps the trp repressor bind to its operator ...
Localization of Protein-Protein lnteractions between Subunits of
Localization of Protein-Protein lnteractions between Subunits of

... Rr value as a relative measure of each fusion protein's dimerization coefficient. Segments encoding pieces of the carboxy-terminal half of oat phytochrome were excised from pCIB315 (Thompson et al., 1989) and inserted into pME10. Initially, an Xmnl fragment encoding amino acids V623-E1048, nearly al ...
Development of the Ruminant Digestive Tract
Development of the Ruminant Digestive Tract

... • Decrease in activity after birth is diet dependent – In ruminant calves, activity drops to mature levels by 8 weeks of age – In pre-ruminant calves, activity at 8 weeks is 10x greater than ...
Predicted function of the vaccinia virus G5R protein
Predicted function of the vaccinia virus G5R protein

... each protein associated with the intracellular mature virion using mass spectrometry were unable to detect G5R within the intracellular mature virion (Yoder et al., 2006; Chung et al., 2006). When we employed a hidden Markov model (HMM) comparison search tool (HHsearch) to screen for the functions o ...
Identification and functional analysis of a prokaryotic-type
Identification and functional analysis of a prokaryotic-type

... The availability of complete genome sequences in plants allowed us to identify single-copy genes in Arabidopsis and rice with similar sequence to the unusual AAT identified in pine, which were named AtAAT and OsAAT respectively. No similar genes were found in animal genomes, suggesting that this aty ...
Purification and some properties of UDP
Purification and some properties of UDP

... chromatography on peptide-Sepharose. The affinity ligand is a synthetic dodeca peptide described by Weilke et al. (1997) modified by replacement of two C-terminal lysine residues by glycine (Table II, peptide 2). Compared with the initially described peptide, no specific interaction with proteins ot ...


... 18. (8 pts) After correcting the mistake in the DNA for the growth hormone (see previous problem), you find that it is impossible to purify the human grown hormone from the complex mixture of proteins in the bacterial cells. Briefly describe how you would fix this problem by one of the following two ...
Heavy Metal Detoxification in Plants
Heavy Metal Detoxification in Plants

... been, unlike with other organisms, no systematic genetic approach to this problem. Comparisons of naturally derived, heavy metal-tolerant plants or laboratory-selected, metal-tolerant plant cell lines with their sensitive counterparts had also failed to identify the underlying mechanisms. In the lat ...
Diverse Adaptations of an Ancestral Gill: A Common Evolutionary
Diverse Adaptations of an Ancestral Gill: A Common Evolutionary

... tubular tracheae, and spinnerets derive from modified epipods/gills of successive opisthosomal segments. The hypothesis that these structures derive from the opisthosomal book gills of aquatic chelicerates dates back to comparative anatomical studies of the late 19th century [5–8], but until now man ...
Thermostable glycerol kinase from a
Thermostable glycerol kinase from a

... The Pk-glpK gene, which encodes glycerol kinase (GK) from a hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1, was cloned and expressed in Escherichia coli. The amino acid sequence of this enzyme (Pk-GK) deduced from the nucleotide sequence showed 57% identity with that of E.coli GK and 47% i ...
Molecular Characterization of NADH-Dependent
Molecular Characterization of NADH-Dependent

... 1989), and alfalfa (Anderson et al., 1989) has been isolated and characterized, and its activity has been found to increase markedly during development of Np-fixing(effective) nodules (Benny and Boland, 1977; Chen and Cullimore, 1988; Anderson et al., 1989). Plant-controlled, non-N2-fixing (ineffect ...
Compartmentation in plant metabolism
Compartmentation in plant metabolism

... the last 40 years have revealed the extensive compartmentation of plant metabolism. In recent years, new protein mass spectrometry and fluorescent-protein tagging technologies have accelerated the flow of information, especially for Arabidopsis thaliana, but the intracellular locations of the majority ...
Neuronal and glial expression of the adhesion - IMBB
Neuronal and glial expression of the adhesion - IMBB

... Expression of the cell adhesion molecule TAG-1 is down-regulated in adult brain, with the exception of certain areas exhibiting structural plasticity. Here, we present evidence that TAG-1 expression persists also in adult rat spinal cord and dorsal root ganglia (DRG), and can be up-regulated after i ...
The Proteins of the Keratin Component of Bird`s Beaks
The Proteins of the Keratin Component of Bird`s Beaks

... Charge heterogeneity of the proteins was studied by electrophoresis on cellulose acetate in urea at pH 8·9 (Blagrove et al. 1975) and in urea (6 M), citric acid (0·2 M) and sodium chloride (0·03 M) at pH2·9. Electrophoresis of the S-carboxymethyl proteins in sodium dodecyl sulphate (SDS)-polyacrylam ...
DNA-binding proteins
DNA-binding proteins

... • Enzyme induction can also be controlled by a repressor • Addition of inducer inactivates a repressor protein (not same as corepressor substance) , and transcription can proceed (Figure 7.8) ...
Adaptative biochemical pathways and regulatory networks in
Adaptative biochemical pathways and regulatory networks in

... fermentation and EPS production, BAS-10 was cultivated: i) under aerobic conditions on Fe(III)citrate (FEC) as sole carbon source; ii) under anaerobic conditions using FEC or iii) Na(I)-citrate (NAC) as sole carbon source. Then, bacterial proteomes were analyzed using anaerobic FEC growth as pivotal ...
Isolation and Purification of RP2-L, a Nuclear Protein Fraction of the
Isolation and Purification of RP2-L, a Nuclear Protein Fraction of the

... ployed (see "Materials and Methods"). Following a small "breakthrough" peak, a single protein peak was eluted by a gradient increasing to l N formic acid with a maximum at fraction 95. The distribution of isotope in the chromatogram in dicates that at least two protein fractions were eluted by a gra ...
by plasmid
by plasmid

... the 5’ ends. They might use some sort of slippage mechanism, using the terminal protein as either a recombinase or a primer or both. ...
Amino acid sequence of an immunogenic corneal stromal protein.
Amino acid sequence of an immunogenic corneal stromal protein.

... acid sequence of CO-Ag to the Ca2+-binding proteins of the S-100 family. The amino acid identity between CO-Ag and the S-100 proteins ranges from 25% (S100L) to 81% (calgraulin C). The S-100 are a group of low molecular weight (approximately 10 kDa) acidic Ca2+-binding proteins. These proteins are e ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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