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Fatty acid transport proteins: a current view of a
Fatty acid transport proteins: a current view of a

... between one and six transmembrane domains30. The subcellular localization of FATP1 in 3T3L1 adipocytes revealed that the protein is found on the plasma membrane and in small vesicles distributed throughout the cytoplasm14. Similarly, we found FATP4 to be localized primarily to the apical plasma memb ...
DOC
DOC

... measure protein turnover in shrimps, and lobsters. The criteria of the technique [6] were fully satisfied. The amino acid composition of the whole body of the shrimp was measured using the method given by Lyndon et al. [7]. In order to gain some insight into the likely amino acid concentrations occu ...
Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell
Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell

... cultures and 830 mmol C l21 for glucose (anaerobic)-limited cultures (residual glucose concentration ,0.3 mM). Glucose-limited cultures were grown at a dilution rate (D) of 0.05 h21 under anaerobic conditions due to the wash-out of glucose (anaerobic)-limited sfp1D cultures at D50.10 h21. Anaerobios ...
Comparative Visualization of Protein Structure
Comparative Visualization of Protein Structure

... There are many methods for quantifying the similarity of individual amino acids. Some methods compare the amino acid sizes, possible charges, bonding patterns, and other chemical properties. [24] The measures based on evolutionary mutation rates do not specifically take structural information into c ...
nitrogen assimilation and global regulation in escherichia coli
nitrogen assimilation and global regulation in escherichia coli

... in Rhizobium leguminosarum bacteroids, which subsequently secrete alanine to the pea plant (1).] ...
(De)regulation of key enzyme steps in the shikimate pathway and
(De)regulation of key enzyme steps in the shikimate pathway and

... Prephenate dehydratase (PDT), chorismate mutase (CM) and 3-deoxy-D-arabino-7-heptulosonate 7-phosphate (DAHP) synthase are key regulatory enzymes in aromatic amino acid biosynthesis in the actinomycete Amycolatopsis methanolica. Deregulated, feedback-control-resistant mutants were isolated by incuba ...
Over-expression of a putative poplar glycosyltransferase gene
Over-expression of a putative poplar glycosyltransferase gene

... several individual poplar glycosyltransferase genes have also been characterized. For example, Zhou et al. (2007) cloned two poplar glycosyltransferase genes, PoGT8D and PoGT43B (belonging to the GT8 and GT43 families, respectively) and found that they were associated with the secondary wall and inv ...
A Mutation in the Anticodon of a Single tRNA Is
A Mutation in the Anticodon of a Single tRNA Is

... supplemented with 2 mM sirtinol (F), 250 nM 2,4-D, or 2 mM of the ethylene precursor ACC (G). Later stage phenotypes are shown in I and J, wild type on the left, heterozygous mutant in the center, and heterozygous recapitulation line on the right. I, Fully formed rosette leaf stage. J, Mature plants ...
Acquisition of Retinoic Acid Signaling Pathway and
Acquisition of Retinoic Acid Signaling Pathway and

... for seeking “difference” between vertebrates and invertebrates, as carefully pointed out by Holland and GarciaFernàndez (1996). In addition, cephalochordate amphioxus and urochordate ascidians belong to the phylum Chordata, together with vertebrates (Kowalevsky, 1866, 1867; Garstang, 1928; Katz, 198 ...
Principles of BIOCHEMISTRY
Principles of BIOCHEMISTRY

... Box 22.1 Some Antibiotics Inhibit Protein Synthesis • Some antibiotics prevent bacterial growth by inhibiting the formation of peptide bonds • Puromycin (next slide) resembles the 3’ end of an aminoacyl-tRNA, and can enter the A site of a ribosome • The peptidyl-puromycin formed is bound weakly in ...
THE PROTEIN NON-FOLDING PROBLEM: AMINO ACID
THE PROTEIN NON-FOLDING PROBLEM: AMINO ACID

... data (Table 1) An argument against use of homology is that such sequences are correlated and so adding sequences by this method does not increase the information content very effectively. However, for many proteins with disordered regions, the disordered parts show significantly less sequence simila ...
www.iplantcollaborative.org
www.iplantcollaborative.org

... BGI – SAGE Analysis of Super Hybrid Rice • Serial Analysis of Gene Expression (SAGE) – 465k tags • “Most of the down-regulated genes in the hybrid were found related to protein processing (maturation and degradation).” ...
Characterization of novel canine bocaviruses and their association
Characterization of novel canine bocaviruses and their association

... bioinformatics analyses and predictions, these observations require experimental validation in subsequent studies. To determine CBoV’s appropriate phylogenetic classification and genetic relatedness to other known parvovirus species, at least one representative virus, as well as the reference genome ...
Projection Structure of a Plant Vacuole Membrane Aquaporin by
Projection Structure of a Plant Vacuole Membrane Aquaporin by

... ®tness and survival (Sanhewe & Ellis, 1996a,b). Immediately after germination, a-TIP may regulate the rehydration of PSVs, osmoregulate the cytoplasm during nutrient export from the PSVs, or adjust vacuolar volume as the central vacuole reforms (reviewed by Maurel et al., 1997). The major intrinsic ...
Identification of genes from flat oyster Ostrea
Identification of genes from flat oyster Ostrea

... quantitative real time PCR. Quantitative real time PCR is commonly used to study gene expression due to high sensitivity, specificity, reproducibility and broad dynamic range [3-5]. Level of gene expression is determined by quantifying the relative amount of target mRNA between different conditions, ...
Proteins
Proteins

... Enzymes represent the widest class of proteins (more than 3000 individual enzymes in the Enzyme Nomenclature and Enzyme Classification). Enzymes act as biological catalysts that accelerate the rates reactions inside the living organism. Each Enzymes are specific in their action and modulate individu ...
Chapter 1 Non-Canonical Amino Acids in Protein Engineering
Chapter 1 Non-Canonical Amino Acids in Protein Engineering

... constitutively or with a promoter orthogonal to the promoter used to express the recombinant protein under study (the “test protein”). Since induction of the test protein occurs in medium supplemented with a non-canonical amino acid, non-orthogonal induction schemes will result in incorporation of t ...
AtPex14p maintains peroxisomal functions by determining protein
AtPex14p maintains peroxisomal functions by determining protein

... exclusively degraded in glyoxysomes (i.e. not in mitochondria) during germination and post-germinative growth (Beevers, 1982). In contrast, leaf peroxisomes are found widely in cells of photosynthetic organs. It has been shown that some of the enzymes responsible for photorespiration are localized i ...
ABCA17P - BMC Molecular Biology
ABCA17P - BMC Molecular Biology

... (A) Structural organization of the human ABCA17P/ABCA3 gene locus on chromosome 16p13.3. Both genes are organized in head-to-head orientation on opposite strands and overlap at their 5' ends. Exons are represented by black (ABCA17P) and gray boxes (ABCA3), respectively, and numbered in 5' to 3' orde ...
University of Groningen Structure and mechanism of the ECF
University of Groningen Structure and mechanism of the ECF

... proteins (BioY, NikM and CbiM) were very different. A link between BioY, FolT and ThiT was made when an orthologue of the bioY gene was found in the L. casei genome, but without the additional bioMN (ecfAT) genes. Instead, an operon encoding BioMNhomologues was found at a different location in the g ...
Additional Methods_Office2004
Additional Methods_Office2004

... reference, by the methods and instruments described in 7. The extinction coefficient of the red form 570 was found by measuring the initial change in optical density (OD) of the red and green forms of protein following irradiation with 385 nm light, assuming in an incremental time period the loss i ...
Know Your Feed Terms - Alberta Agriculture and Forestry
Know Your Feed Terms - Alberta Agriculture and Forestry

... Probiotics – a probiotic can be a live (viable) culture of microbial species, a dead (non-viable) product of microbial fermentation or an extract of plant origin. The function of a probiotic is to improve the growth and development of the normal, desirable microbial population in the gut, allowing t ...
PDF - BMC Genomics
PDF - BMC Genomics

... in protein domain boundary prediction, transductive support vector machine (SVM) and multi-layered perceptron (MLP). Second, in the model comparison, the effectiveness of hydrophobicity information presented in Evolutionary and Hydrophobicity profile (EH-profile) is thoroughly tested and compared wi ...
Lipids 44:
Lipids 44:

... ARA constitutes about 60% of total fatty acids (TFA) and over 95% of cellular ARA is deposited in TAG in cytoplasmic lipid bodies [12]. The biosynthesis of VLC-PUFAs in algae follows various pathways, initiating from oleic acid exported from the chloroplast and employing polar extraplastidial lipids ...
Nutrition for Strength/Power Athletes
Nutrition for Strength/Power Athletes

... • There are a large number of research studies indicating that intake of protein is advantageous for athletes when muscle hypertrophy is required. ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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