Gene clusters for β-lactam antibiotics and control of their expression
... second ring in β-lactam molecules, the precursors and biosynthetic pathways of clavams, carbapenems and monobactams differ from those of penicillins and cephalosporins. These last two groups, including cephamycins and cephabacins, are formed from three precursor amino acids that are linked into the ...
... second ring in β-lactam molecules, the precursors and biosynthetic pathways of clavams, carbapenems and monobactams differ from those of penicillins and cephalosporins. These last two groups, including cephamycins and cephabacins, are formed from three precursor amino acids that are linked into the ...
A Bayesian network model for protein fold and remote homologue
... were shown to be three times more effective than pairwise methods at detecting remote protein homologies. Further improvement in the sensitivity of remote homologue classification has been obtained by Jaakkola et al. (1999) who have used sequence-based HMMs to develop a discriminative model, based o ...
... were shown to be three times more effective than pairwise methods at detecting remote protein homologies. Further improvement in the sensitivity of remote homologue classification has been obtained by Jaakkola et al. (1999) who have used sequence-based HMMs to develop a discriminative model, based o ...
Full text in pdf - International Microbiology
... second ring in β-lactam molecules, the precursors and biosynthetic pathways of clavams, carbapenems and monobactams differ from those of penicillins and cephalosporins. These last two groups, including cephamycins and cephabacins, are formed from three precursor amino acids that are linked into the ...
... second ring in β-lactam molecules, the precursors and biosynthetic pathways of clavams, carbapenems and monobactams differ from those of penicillins and cephalosporins. These last two groups, including cephamycins and cephabacins, are formed from three precursor amino acids that are linked into the ...
FTIR Analysis of Protein Structure
... This leads to an intermediate level of protein structure called secondary structure. The types of secondary structure includes the αhelices and βsheets, which allow the amides to hydrogen bond very efficiently with one another. Both are periodic structures. In an α-helix the polypeptide backbone is ...
... This leads to an intermediate level of protein structure called secondary structure. The types of secondary structure includes the αhelices and βsheets, which allow the amides to hydrogen bond very efficiently with one another. Both are periodic structures. In an α-helix the polypeptide backbone is ...
AN ABSTRACT OF THE THESIS OF Sara R. Fassio for the degree of
... transcription factors AphA and AphB, which positively regulate transcription of the twocomponent regulatory system tcpPH. The membrane-bound protein complex TcpPH works with the membrane-bound protein complex ToxRS to activate transcription of toxT, which encodes an AraC-type transcriptional factor ...
... transcription factors AphA and AphB, which positively regulate transcription of the twocomponent regulatory system tcpPH. The membrane-bound protein complex TcpPH works with the membrane-bound protein complex ToxRS to activate transcription of toxT, which encodes an AraC-type transcriptional factor ...
Spermatogenesis-preventing substance - Development
... Male eels were kept in 500 l circulating fresh-water tanks at 23°C. A single injection of human chorionic gonadotropin (hCG) dissolved in saline (150 mM NaCl) was administered to the eels intramuscularly at a dose of 5 IU per body weight. Fish were either sacrificed immediately or at 1, 3, 6, 9, 12, ...
... Male eels were kept in 500 l circulating fresh-water tanks at 23°C. A single injection of human chorionic gonadotropin (hCG) dissolved in saline (150 mM NaCl) was administered to the eels intramuscularly at a dose of 5 IU per body weight. Fish were either sacrificed immediately or at 1, 3, 6, 9, 12, ...
ZRII ACHIEVE™ PRODUCT INFORMATION
... Soy is naturally high in protein, rich in complex carbohydrates, high in fiber, and free of lactose, making it a good protein choice for many people. Achieve uses only soy protein from certified non-GMO soybeans, which means our soy is free of genetically modified organisms. Soy also has an impressi ...
... Soy is naturally high in protein, rich in complex carbohydrates, high in fiber, and free of lactose, making it a good protein choice for many people. Achieve uses only soy protein from certified non-GMO soybeans, which means our soy is free of genetically modified organisms. Soy also has an impressi ...
SPINDLY, a tetratricopeptide repeat protein involved in
... but not completely epistatic to gal-2. spy-4 gal-2 double mutants do not require exogenous GA for seed germination, petal development, and male fertility; however, spy-4 gal-2 double mutant plant height is less than that of spy-4 single mutants. We have also found that, similar to the spy-i gal-2 mu ...
... but not completely epistatic to gal-2. spy-4 gal-2 double mutants do not require exogenous GA for seed germination, petal development, and male fertility; however, spy-4 gal-2 double mutant plant height is less than that of spy-4 single mutants. We have also found that, similar to the spy-i gal-2 mu ...
Non-coding RNAs - Structural Biology Labs
... RNA results in proteins? • Of all RNA, transcribed in higher eukaryotes, 98% are never translated into proteins • Of those 98%, about 50-70% are introns • The rest originate from non-protein genes, including rRNA, tRNA and a vast number of other non-coding RNAs (ncRNAs) • Even introns have been show ...
... RNA results in proteins? • Of all RNA, transcribed in higher eukaryotes, 98% are never translated into proteins • Of those 98%, about 50-70% are introns • The rest originate from non-protein genes, including rRNA, tRNA and a vast number of other non-coding RNAs (ncRNAs) • Even introns have been show ...
Cloning, Sequencing, and Characterization of the Pradimicin
... The angucycline cyclase JadI is responsible for catalyzing the cyclization of the fourth ring (the final ring of jadomycin) [15]. PrmL shares sequence similarity to FdmE (67% identity), GrhS (58% identity), and TcmJ (55% identity). The roles of FdmE, GrhS, and TcmJ are not clearly understood, but th ...
... The angucycline cyclase JadI is responsible for catalyzing the cyclization of the fourth ring (the final ring of jadomycin) [15]. PrmL shares sequence similarity to FdmE (67% identity), GrhS (58% identity), and TcmJ (55% identity). The roles of FdmE, GrhS, and TcmJ are not clearly understood, but th ...
Bioinformatics of proteins: Sequence, structure and the `symbiosis
... by one or more ordered assemblies of molecular functions. Examples of biological process terms are signal transduction or pyrimidine metabolism. It can be difficult to distinguish between a biological process and a molecular function, but the general rule is that a process must have more than one di ...
... by one or more ordered assemblies of molecular functions. Examples of biological process terms are signal transduction or pyrimidine metabolism. It can be difficult to distinguish between a biological process and a molecular function, but the general rule is that a process must have more than one di ...
Extended Detail on Checklist items
... sequences of length ≥9 amino acids. When weaker evidence is offered for a previously unreported protein or a coding element proposed translation product, justify that other peptides cannot be expected. Single-peptide detections simply have too high a chance of being some type of pernicious false pos ...
... sequences of length ≥9 amino acids. When weaker evidence is offered for a previously unreported protein or a coding element proposed translation product, justify that other peptides cannot be expected. Single-peptide detections simply have too high a chance of being some type of pernicious false pos ...
in Graminaceous Plants
... sion vector for yeast, and has the ADH (alcohol dehydrogenase) promoter driving expression of the introduced cDNAs. The cDNA library was screened by colony hybridization using the PCR product (probe I, see Fig. 2). The probe was labeled with [a-32P]dATP using a randomprimer labeling kit (version 2, ...
... sion vector for yeast, and has the ADH (alcohol dehydrogenase) promoter driving expression of the introduced cDNAs. The cDNA library was screened by colony hybridization using the PCR product (probe I, see Fig. 2). The probe was labeled with [a-32P]dATP using a randomprimer labeling kit (version 2, ...
Prediction of mitochondrial proteins of malaria parasite
... malaria. In past, number of methods, including multi-subcellular localization and mitochondria specific, has been developed (Guda et al. 2004; Kumar et al. 2006). It is likely that these methods developed for eukaryotic proteins will also be valid for malaria parasite. Recently, it has been shown in ...
... malaria. In past, number of methods, including multi-subcellular localization and mitochondria specific, has been developed (Guda et al. 2004; Kumar et al. 2006). It is likely that these methods developed for eukaryotic proteins will also be valid for malaria parasite. Recently, it has been shown in ...
Volume 18 - Number 9 September 2014 Atlas of Genetics and Cytogenetics
... contains a proline rich, SH3 domain binding motif, three tyrosine containing SH2 domain binding sites (Xu et al., 2007), of which a YXXM motif is for PI3 kinase subunit p85 binding (Lodyga et al., 2009). In the middle region, there are two pleckstrin homology domains and another tyrosine binding mot ...
... contains a proline rich, SH3 domain binding motif, three tyrosine containing SH2 domain binding sites (Xu et al., 2007), of which a YXXM motif is for PI3 kinase subunit p85 binding (Lodyga et al., 2009). In the middle region, there are two pleckstrin homology domains and another tyrosine binding mot ...
Phosphate binding sites identification in protein
... methods exist for predicting the binding sites of a variety of ligands such as various metal ions or carbohydrates (7–11). However, to the best of our knowledge, no method is yet available for the identification of phosphate binding sites (PbSs) even if the biological relevance of this specific ligand ...
... methods exist for predicting the binding sites of a variety of ligands such as various metal ions or carbohydrates (7–11). However, to the best of our knowledge, no method is yet available for the identification of phosphate binding sites (PbSs) even if the biological relevance of this specific ligand ...
Word - Food Standards Australia New Zealand
... two traits were combined by crossing the two GM cotton lines using conventional breeding to produce cotton line MXB-13. Using two B. thuringiensis derived insecticidal proteins, rather than one, in the same plant improves the spectrum of control, the seasonal efficacy and significantly reduces the c ...
... two traits were combined by crossing the two GM cotton lines using conventional breeding to produce cotton line MXB-13. Using two B. thuringiensis derived insecticidal proteins, rather than one, in the same plant improves the spectrum of control, the seasonal efficacy and significantly reduces the c ...
The Arabidopsis stearoyl-acyl carrier protein-desaturase
... et al. 2001; Kachroo et al. 2001). We have previously shown that a reduction in 18:1 levels is the likely cause of altered defense signaling in ssi2 plants (Kachroo et al. 2001, 2003a, b, 2004, 2005). The altered morphology, as well as defense-related phenotypes in ssi2, can be restored by elevating ...
... et al. 2001; Kachroo et al. 2001). We have previously shown that a reduction in 18:1 levels is the likely cause of altered defense signaling in ssi2 plants (Kachroo et al. 2001, 2003a, b, 2004, 2005). The altered morphology, as well as defense-related phenotypes in ssi2, can be restored by elevating ...
Side-chain hydrophobicity scale derived from transmembrane
... to the total thermodynamic stability of transmembrane proteins (1, 2). A hydrophobicity scale that ranks the water-to-bilayer transfer energies of the twenty natural amino acid side chains could allow the identification of genes that code for membrane proteins (3–6) and aid the understanding of how ...
... to the total thermodynamic stability of transmembrane proteins (1, 2). A hydrophobicity scale that ranks the water-to-bilayer transfer energies of the twenty natural amino acid side chains could allow the identification of genes that code for membrane proteins (3–6) and aid the understanding of how ...
food derived from insect-protected, glufosinate ammonium
... two traits were combined by crossing the two GM cotton lines using conventional breeding to produce cotton line MXB-13. Using two B. thuringiensis derived insecticidal proteins, rather than one, in the same plant improves the spectrum of control, the seasonal efficacy and significantly reduces the c ...
... two traits were combined by crossing the two GM cotton lines using conventional breeding to produce cotton line MXB-13. Using two B. thuringiensis derived insecticidal proteins, rather than one, in the same plant improves the spectrum of control, the seasonal efficacy and significantly reduces the c ...
Tubulin Subunit Carboxyl Termini Determine Polymerization Efficiency
... acidic proteins, electrode buffer (0.1%) was lauryl sulfate (Sigma) or equivalent (14). but the fl subunit is slightly more acidic than a! (apparent PI Following electrophoresis, gels were stained with Coomassie Brilliant Blue R-250, destained, and air-dried (15). The migration of tubulin = 5.3 (p), ...
... acidic proteins, electrode buffer (0.1%) was lauryl sulfate (Sigma) or equivalent (14). but the fl subunit is slightly more acidic than a! (apparent PI Following electrophoresis, gels were stained with Coomassie Brilliant Blue R-250, destained, and air-dried (15). The migration of tubulin = 5.3 (p), ...
Sequence and transcription analysis of the Petunia mitochondrial
... upstream of the translation start site. The 5' terminus of the longest transcript maps to the sequenoe ATATAGTA, which is nearly identical to the yeast mitochondrial transcription initiation site ATATAAGTA. Primer extension analysis indicates that these two shorter transcripts are not due to splioin ...
... upstream of the translation start site. The 5' terminus of the longest transcript maps to the sequenoe ATATAGTA, which is nearly identical to the yeast mitochondrial transcription initiation site ATATAAGTA. Primer extension analysis indicates that these two shorter transcripts are not due to splioin ...
Effects of Transition Mutations in the Regulatory
... carry only the mutation at position 732; but even though we can calculate that several of these should have arisen we have been unable to find any recombinants with a distinctive Spo phenotype (M. D. Yudkin, unpublished work). Thirdly, genetic mapping unequivocally places spo-69 on the lys-distal si ...
... carry only the mutation at position 732; but even though we can calculate that several of these should have arisen we have been unable to find any recombinants with a distinctive Spo phenotype (M. D. Yudkin, unpublished work). Thirdly, genetic mapping unequivocally places spo-69 on the lys-distal si ...
Transcriptional activity and role of plasmids of
... Approximately 64% of the Pc34434568 genome was significantly DE (SDE; P-adjusted < 0.1) during growth in 35 BU hop extract, whereas only 23% of the Lb464 genome was SDE during growth in 50 BU hop extract (Table 1). Furthermore, a higher number of highly SDE genes was seen for Pc344 than for Lb464, w ...
... Approximately 64% of the Pc34434568 genome was significantly DE (SDE; P-adjusted < 0.1) during growth in 35 BU hop extract, whereas only 23% of the Lb464 genome was SDE during growth in 50 BU hop extract (Table 1). Furthermore, a higher number of highly SDE genes was seen for Pc344 than for Lb464, w ...